Dear All,
Big news flash there,
but it seems to be applicable and a better than TBE and TAE buffers, 10mM Sodium boric acid is used to separate bands up to 5Kbp.
less heat is generated compared to the regular buffers, thus, the voltage and be increased, reducing the time required for separation.
I'm going to imply this in my lab, yet, I'm confused about the ph of the boric acid and the agarose
regarding the latter, a low electroendosmosis agarose should be used, but what is a low EEO agarose anyway, cause the agarose we are using now has an eeo of .09 - .13 compared to an agarose labeled low EEO that its value is 0.1 - 0.15.
So far, I have to get some sodium boric acid, prepare it in a molarity of 10mM (ph ????) and run a gel.
Any more info on the subject is appreciated, I feel this method should be more "out there".
Regards.