Zuyao Li's research works | Guilin University of Technology and other places

UV‐Vis absorption spectrum of PBI (10 μM). (A) Different metal ions (1.0 eq.). Inset: Photographs of other cations and Cu²⁺ colour changes added to PBI solution under 310 nm light. (B) Add Cu²⁺ (0∼0.5 eq.) in buffer solution.

(A) The histogram shows the changes in fluorescence intensity of PBI (10 μM) in HEPES buffered solution (10 mM, pH 7.4) at the presence of different ions (1.0 eq.).The yellow bar represents the normalized fluorescence intensity of PBI, while the green bar represents the changes in fluorescence intensity in the presence of different metal ions.(B) Fluorescence intensity at 385 nm of PBI (10 μM) with 10 μM various metal ions in the absence of Cu²⁺ (yellow bars) or the presence of Cu²⁺ (green bars).

Fluorescence spectrum of PBI (10 μM). (A) After adding different amounts of Cu²⁺ (0–1.0 eq.), inset: After adding Cu²⁺ (0–0.5 eq.), the emission intensity of PBI at 385 nm. (B) Linear relationship at 385 nm with different amounts of Cu²⁺ (0–0.2 eq.) in buffer solution.

(A) Chromaticity coordinates of fluorescence emission of probe after adding Cu²⁺. (B) Determination of Job′s plot curves of binding stoichiometry for PBI and Cu²⁺ in HEPES buffer solution (10 mM, pH 7.4) with total concentrations of PBI and Cu²⁺ of10 μM, respectively.

(A) the B−H plot between 1/(A−A0) and (1/Cu²⁺) 1/2 concentration(λabs=332 nm). (B) the B−H plot between 1/(F−F0) and (1/Cu²⁺) 1/2 concentration(λem=385 nm).

A Selective and Reversible Fluorescent Probe for Cu and GSH Detection in Aqueous Environments

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March 2023

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Cu²⁺ and Biothiols play a critical role in diverse biological processes in organisms. Herein, we reported using a well‐known ESPT molecule 2‐(2’‐Pyridyl)benzimidazol(PBI) as a fluorescent probe to detect Cu²⁺ and GSH in aqueous solution. PBI presented a rapid fluorescence “ON‐OFF” response on Cu²⁺ over other metal cations, with a low detection limit (39.4 nM), and also been applied in real water samples detection. Through mass spectroscopy, single crystal X‐ray diffraction and DFT calculation, we confirmed that two PBI molecules would form a stable complex with one Cu²⁺. After adding GSH, PBI‐Cu²⁺ complex would release the free probe, and the “on‐off‐on” recognition process to achieve continuous detection of Cu²⁺ and GSH.

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Zuyao Li’s research while affiliated with Guilin University of Technology and other places

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