Zuoyi Zhu's research while affiliated with Zhejiang Academy of Agricultural Sciences and other places

Publications (29)

Article
In this study, a high performance liquid chromatography-electrochemical detection (HPLC-ECD) method has been developed for the sensitive determination of 22 phenolic compounds in extra virgin olive oil (EVOO). Chromatographic separation was performed on an Eclipse XDB-C18 column with a gradient methanol/aqueous phosphoric acid elution, and the ECD...
Article
Extra-virgin olive oil (EVOO) is an important imported commercial product in China. This study comprehensively evaluated the quality of EVOOs imported from different countries into China based on chemical parameters and sensory attributes. Multivariate statistics were used to authenticate their geographical origins. 71.8% of the oils failed to meet...
Article
Asteris Radix et Rhizoma (AR) has been widely used as a herbal medicine for treating various symptoms and possesses a number of bioactivities. A neutral polysaccharide ARP-1 was isolated from AR with weight-average molecular weight of 214 kDa. The heteropolysaccharide ARP-1 was composed of fucose, arabinose, galactose, glucose and mannose with a mo...
Article
Full-text available
Black apple is a new elaborated product obtained from whole fresh apple through fermentation at controlled high temperature (60~90°C) and humidity (relative humidity of 50~90%). The appearance, color, texture, and taste of black apple changed dramatically compared with those of fresh apple. In this study, changes in the physicochemical and phytoche...
Article
Full-text available
To screen several peptides that could possibly inhibit the biosynthesis of cholesterol, we selected peptides with high 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGCR) inhibitory activity from silkworm pupae protein hydrolysates (SPPHs). SPPH was obtained by hydrolysing the proteins with neutral proteinase, and filtered to <3, 3–5 and >5 kD...
Article
Full-text available
Silkworm pupae are a kind of insect resource food that rich of good quality protein. To identify the peptide with high antioxidant activity from silkworm pupae protein hydrolysates, and provide the basis for the application of silkworm pupae protein hydrolysates and antioxidant peptides in functional foods, silkworm pupae proteins were hydrolysed b...
Article
Schematic of the procedure for structural characterisation and antioxidant activity of melanoidins from high‐temperature fermented apple. High‐temperature fermented apple is a new fermented product with characteristic black appearance, which is prepared through natural fermentation of whole fresh apple at controlled high temperature (60–90 °C) and...
Article
An on-line two-step pyrolytic methylation technique combined with gas chromatography was developed for rapid analysis of free and bonded fatty acids in Polysorbate 80 respectively. Free fatty acids in Polysorbate 80 were transformed to their corresponding fatty acid methyl esters in the presence of tetramethylammonium acetate (TMAAc) in the first s...
Article
A novel solid phase microextraction coupled with flash evaporation gas chromatography method was applied to the determination of phthalate esters (PAEs). Polysulfone (PSF) hollow fiber at 1cm length was employed as extraction element to adsorb PAEs directly. Predominant parameters including stirring velocity, salt concentration, extraction time and...
Article
In this work, a simple sugaring-out assisted liquid-liquid extraction (SULLE) method coupled with high performance liquid chromatography-electrochemical detection (HPLC-ECD) has been developed for rapid and sensitive determination of 17 phenolic compounds in honey. To achieve the maximum extraction efficiency of target analytes, several parameters,...
Article
An ion chromatography coupled with pulsed amperometric detection (IC-PAD) method was developed for the determination of sugars in royal jelly (RJ). The method enables quantification of 19 sugars with higher sensitivity than HPLC method. Good linearity was observed in the range of 0.02–500 mg L –1 with coefficients of determination (R ² ) ≥ 0.9994....
Article
A method was developed for the simultaneous determination of anserine, homocarnosine and carnosine in meat samples using ion chromatography (IC) with integrated pulsed amperometric detection (IPAD). The samples were separated on a high performance anion exchange AminoPac PA10 column (250 mm×2 mm) using 100 mmol/L NaOH as the eluent. The flow rate w...
Article
An approach for preparation of a novel composite anion exchanger composed of polystyrene/divinylbenzene (PS/DVB) beads and quaternized nanodiamods (QND) were proposed. Oxidized nanodiamonds (OND) were quaternized by the condensation polymerization between methylamine (MA) and 1,4-butanediol diglycidyl ether (BDDE), which were characterized by Fouri...
Article
A two-dimensional ion chromatography system was developed for the determination of γ-hydroxybutyrate (GHB) in human urine samples. Ion exclusion chromatography was used in the first dimensional separation for elimination of urine matrices and detection of GHB above 10mgL(-1), ion exchange chromatography was used in the second dimensional separation...
Article
An on–line pyrolytic butylation approach was proposed to determine methacrylic acid (MA) in food simulants by gas chromatography (GC) without an expensive pyrolyzer. MA in food simulants was converted into butyl methacrylate in the presence of tetrabutylammonium hydroxide (TBAH) without any pretreatment at 330 °C in the injection–port, contributing...
Article
The objectives of this study were to identify peptides that inhibit α-glucosidase using a quantitative structure-activity relationship (QSAR) screening method and a database of silkworm peptides. This study compared the docking characteristics of several peptides with high inhibitory activity against α-glucosidase and summarized the molecular mecha...
Article
An ion chromatographic approach with on-line sample pretreatment column-switching technique was proposed for the determination of anions and cations in biodiesel. By the employment of the column-switching technique combined with a polymer-based reversed-phase column (An IonPac NG1 guard column), organic residues in the aqueous extracts of biodiesel...
Article
An in silico screening method for novel dipeptides from a dipeptide training set was performed. A 3D pharmacophore model with three H-bond acceptor projections (Acc2), and one H-bond donor projection (Don2) was obtained. To validate the pharmacophore model, a test set containing 10 reported dipeptides was screened and all were found to be bioactive...
Article
A novel Cu nanoparticles/reduced graphene oxide-chitosan (CuNPs/r-GO-chitosan) composite film modified glassy carbon electrode (GCE) was fabricated by dispersing CuNPs uniformly on a stable r-GO-chitosan thin film through electrodeposition process. The modified electrode was characterized by cyclic voltammetry, scanning electron microscopy (SEM) an...
Article
Full-text available
The application of ion chromatography with the column-switching technique for the simultaneous analysis of peroxydisulfate and conventional inorganic anions in a single run is described. With this method, conventional inorganic anions were separated by consecutive elution through both the guard column and separation column, but peroxydisulfate that...
Article
Full-text available
A new analytical method has been developed and validated for the simultaneous determination of pesticides from different classes using ion chromatography-online photochemical derivatisation-fluorescence detector (IC-hv-FD). Fluorimetric detection was performed at λex/λem=332 nm/367 nm for imidacloprid and then detector was set at λex/λem=247 nm/470...
Article
A highly sensitive electrochemical sensor was fabricated by electropolymerizing p-aminobenzene sulfonic acid (ABSA) on quaternary amine functionalized multi-wall carbon nanotubes (Q-MWNTs) modified glassy carbon electrode (GCE). Q-MWNTs provide a conductive and stable network for the poly-ABSA (PABSA) immobilization. The voltammetric behaviors of m...
Article
Full-text available
A simple ion chromatographic (IC) method for simultaneous determination of glucose, d-gluconic acid (DGA), 2-keto-d-gluconic acid (2-KDG) and 5-keto d-gluconic acid (5-KDG) was proposed, with pulsed amperometric detection (PAD) and column-switching technique. Using this technique, the four compounds were detected simultaneously in a short time with...
Article
An ion chromatography (IC) system with on-line sample pretreatment using valve-switching technique was developed for the determination of hexavalent chromium (Cr(VI)) in Colla corii asini. Colla corii asini is a complicated sample with organics as main matrix. In this work, a polymer-based reversed-phase column was used as a pretreatment column. Vi...
Article
A simple and sensitive method was developed for simultaneous determination of methotrexate (MTX) and folic acid (FA) by ion chromatography with electrochemical detection (IC-ECD). Quaternary amine functionalized multi-wall carbon nanotubes (q-MWNTs) modified glassy carbon electrode (GCE) was used as an amperometric sensor to determine MTX and FA. T...
Article
A novel composite film modified electrode was prepared by combining the strong electrostatic adsorption ability of positively quaternized carbon nanotubes (q-CNTs) and excellent electroactivity of ionic liquid (1-methylimidazolium trifluoroacetate) doped polyaniline (IL-PANI) for the determination of ascorbic acid (AA). The q-CNTs/IL-PANI/GCE was c...
Article
Full-text available
A simple ion chromatographic method for simultaneous detection of iodide and iodate in a single running was proposed, with columns packed with homemade functionalized polystyrene-divinylbenzene (PS-DVB) resins and column-switching technique. Homemade resins were functionalized with controllable amounts of quaternary ammonium groups. The low-capacit...

Citations

... Olive oil is highly appreciated worldwide due to its unique and distinct flavor, recognized nutritional properties, and beneficial health effects. Due to its price and increasing demand by consumers, extra virgin olive oil (EVOO) is one of the most prone to mislabeling [1]. Several fraud practices have been detected in the olive oil market, including the mislabeling of the oil category level or origin (cultivar, region, and production mode), and the deliberated incorporation of other edible oils. ...
... The band at 1642 cm −1 was attributed to the scissoring vibration of bound water [28]. The absorption peaks around 1366 cm −1 were due to the C-H deformation vibration [29]. The peaks around 1200-1000 cm −1 were attributed to C-O-H side groups and C-O-C glycosidic bond vibrations, suggesting the presence of pyran rings [30]. ...
... As reported by Yang et al. [15], fermentation by LAB usually leads to a decrease in pH value of juice. After 48 h of fermentation, the total acid content of apple juice showed an overall upward trend ( Figure 2B), and there was no significant difference between pasteurized and HHP treated cloudy apple juice [25]. LAB uses malic acid for growth and metabolism, converting it to pyruvic acid and then to lactic acid, which enriches the flavor and taste of apple juice. ...
... Medical studies have shown that consumption of silkworm pupae protein may be associated with a reduced risk of many diseases, such as cardiovascular diseases (hypertension and hyperlipidemia) [70][71][72][73][74][75], and cancer (hepatoma and gastric cancer) [76,77]. Additionally, enzymatically produced silkworm pupa hydrolysate is reported to possess several bioactive properties, such as inhibiting angiotensin-I converting enzyme (ACE) activity [70][71][72][73][74], antioxidant activity [78][79][80], immunomodulatory activity [81], improving hypercholesterolemia [75], and anti-tumor activity [76,77]. ...
... It is remarkably appreciated and included in many people's daily diet [1][2][3]. Fruits fermentation produces a bio liquid that contains several functional molecules [4,5] such as organic acids [5,6], polyphenols [7,8], melanoidins [9,10], and tetramethylpyrazine [11,12]. The production technique used has an impact on the quality of vinegar, while the vinegar-making process plays an important role in the removal and/or formation of new components. ...
... Before treatment, all samples were melted and homogenized at 35 °C. Zhu's methods were used to remove sugar from the CH [27]. Then, a 1 g CH sample was mixed with 2 mL 10% NaCl hydrochloric acid solution (pH = 2) and 2 mL acetonitrile. ...
... In the past 10 years, enzymatic hydrolysis has been frequently adopted as a means of producing bioactive peptides from the proteins of edible insects. In such studies, silkworm pupae were relatively popular for the purpose of bioactive peptide discovery [29][30][31][32][33]. Figure 1 shows a general workflow employed by many studies in the discovery of bioactive peptides. employed in such studies are summarized. ...
... The existing methods for the detection of PAEs in edible oil include gas chromatography (GC), gas chromatographymass spectrometry (GC-MS), high performance liquid chromatography (HPLC), and liquid chromatography-mass spectrometry (HPLC-MS) (Cheng and Chang 2016;Sajid et al. 2016;Zhou et al. 2016a;Huang et al. 2020). For PAEs with high boiling point and weak polarity, HPLC is a more suitable analytical method. ...
... sucrose) and trisaccharides (e.g. erlose) (Daniele & Casabianca, 2012;Wytrychowski et al., 2013;Zhu et al., 2019). With these nutritional and functional components, RJ exhibits a wide variety of pharmacological properties such as antiaging, anti-inflammatory, antitumor, and immunomodulating effects (Collazo et al., 2021;Guo et al., 2021;Uversky et al., 2021). ...
... with discernible isolation between fluoride and the water dip and their column efficiencies were in the range of 54,000-79,800 plates m −1 . Compared with recently reported polymeric AEC packings as summarized in Table 2 [7,13,[32][33][34][35][36][37][38][39], the efficiencies and peak symmetry on our column (79,800 N/m and 1.02 for Cl − ) was superior to that on the best polymeric packing known (68,000 N/m and As: 1.3) [35], and the analysis time (< 5 min) for above six inorganic anions excluding PO 4 3− was also shortest. Their basic separation performance was also provably better than those of some commercial latex-agglomerated columns such as Ionpac AS18 and Ionpac AS11-HC-4 μm ( Table 1) (https:// assets. ...