Zu-yan Gan’s research while affiliated with Guizhou University and other places

Somatic embryogenesis is a unique method of in vitro regeneration, which can be used in plant reproduction, germplasm conservation, and molecular-assisted breeding. The results showed that the optimum medium for embryogenic callus induction was MS + 6 mg L⁻¹ 6-BA + 1.5 mg L⁻¹ TDZ + 0.5 mg L⁻¹ NAA + 30 g L⁻¹ sucrose + 7 g L⁻¹ agar, and the induction rate was 47.45%. The best somatic differentiation medium was MS + 2 mg L⁻¹6-BA + 1.5 mg L⁻¹ TDZ + 30 g L⁻¹ sucrose + 7 g L⁻¹ agar, and the induction rate of somatic embryos was 54.45%. The optimum medium for embryoid proliferation was MS + 6 mg L⁻¹ 6-BA + 1 mg L⁻¹ NAA + 0.2 mg L⁻¹ TDZ, and the proliferation rate and the multiplication coefficient reached 46.33% and 7.83, respectively. The mature somatic embryos were put into MS, B5, and 1/2MS medium for seedling culture. In MS medium, true leaves grew, complete plants were obtained, and the seedling rate was 88.00%. At the same time, the survival rate of transplanting seedlings in the mixed matrix (peat: organic fertilizer: soil = 1:1:1) was as high as 98%. Cytological observation showed that the somatic embryos underwent globular, heart-shaped, torpedo, and cotyledon stages. This study established a regeneration system of C. × generalis with excellent somatic embryos, and provided basic technical support for the large-scale commercial propagation and germplasm resources protection. It will lay a foundation for further research on gene function and breeding new varieties and ideal research materials for the study of somatic embryogenesis mechanism and genetic transformation of C. × generalis.

Somatic embryogenesis is a unique method of in vitro regeneration, which can be used in plant reproduction, germplasm conservation, and molecular-assisted breeding. The results showed that the optimum medium for embryogenic callus induction was MS+6 mg L ⁻¹ 6-BA+1.5 mg L ⁻¹ TDZ+0.5 mg·L ⁻¹ NAA+30 g·L ⁻¹ sucrose +7 g·L ⁻¹ agar, and the induction rate was 47.45%. The best somatic differentiation medium was MS+2 mg·L ⁻¹ 6-BA+1.5 mg·L ⁻¹ TDZ+30g·L ⁻¹ sucrose +7g·L ⁻¹ agar, and the induction rate of somatic embryos was 54.45%. The optimum medium for embryoid proliferation was MS +6mg·L ⁻¹ 6-BA + 1 mg·L ⁻¹ NAA +0.2mg·L ⁻¹ TDZ, and the proliferation rate and the multiplication coefficient reached 46.33% and 7.83, respectively. The mature somatic embryos were put into MS, B5, and 1/2MS medium for seedling culture. T In MS medium, true leaves grew, complete plants were obtained, and the seedling rate was 88.00%. At the same time, the survival rate of transplanting seedlings in the mixed nutrient soil with the ratio of original soil (peat: organic fertilizer: soil) =1:1:1 was as high as 98%. Cytological observation showed that the somatic embryos underwent globular, heart-shaped, torpedo, and cotyledon stages. This study established a tissue culture and regeneration system of C. × generalis with excellent somatic embryos, and provide basic technical support for the large-scale commercial propagation and germplasm resources protection. It will lay a foundation for further research on gene function and breeding new varieties and ideal research materials for the study of somatic embryogenesis mechanism and genetic transformation of C. × generalis.