Zonghu Han’s research while affiliated with University of Minnesota Duluth and other places

... 101 Several studies have demonstrated that the colloidal and thermal stability of MNPs in VS55 can be maintained though surface coating with resorcinol-formaldehyde resin or silica. [104][105][106][107] Additionally, surface modification with poly(ethylene glycol) has been shown to reduce cellular interactions and thus cytotoxicity. 106,107 Manuchehrabadi achieved nanowarming of porcine arteries and porcine aortic heart valve leaflet tissues using magnetic heating. ...

Reference:

Advances in magnetic nanoparticles for molecular medicine

... 101 Several studies have demonstrated that the colloidal and thermal stability of MNPs in VS55 can be maintained though surface coating with resorcinol-formaldehyde resin or silica. [104][105][106][107] Additionally, surface modification with poly(ethylene glycol) has been shown to reduce cellular interactions and thus cytotoxicity. 106,107 Manuchehrabadi achieved nanowarming of porcine arteries and porcine aortic heart valve leaflet tissues using magnetic heating. ...

Reference:

Advances in magnetic nanoparticles for molecular medicine

... Researchers have effectively used different combinations of these CPAs, particularly EG and DMSO, on a variety of tissues and cells, including amnion-derived MSCs [39], cord blood [40], and embryos [41]. High concentrations of CPAs are necessary for the equilibrium vitrification process, which can potentially be detrimental to cells [42]. Consequently, the vitrification freezing method often requires both careful preparation of the CPA mixture and the gradual injection of the CPA at lower temperatures to ensure maximum safety. ...

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Effects, methods and limits of the cryopreservation on mesenchymal stem cells

... Hence, the minimum CPA concentration for vitrification would be ~62% w/w, which is slightly lower than M22 (~66%w/w which includes carrier solution), where we have shown successful vitrification at 3L. Higher concentrations of CPAs such as VS83 (83% w/w CPA) have even lower CCR and can be more easily vitrified but increase biological toxicity relative to the CPAs chosen here [34]. To remain at a lower concentration of CPA and still achieve vitrification at higher volumes without toxicity, future work can assess the impact of ice recrystallization inhibitors (IRIs), polymers (e.g., polyglycerol-PGL, polyvinyl alcohol-PVA, polyethylene glycol-PEG, x-1000, z-1000, etc.), or other novel cryoprotective agents [35,36]. ...

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Physical vitrification and nanowarming at human organ scale to enable cryopreservation

... A subsequent study demonstrated that vitrified rat kidneys recovered through nanowarming were suitable for transplantation and restored full renal function in nephrectomized recipients. 108 Although nanowarming technology is still in its infancy, these studies show great promise to transform the landscape of organ transplantation. Magnetogenetics. ...

Reference:

Advances in magnetic nanoparticles for molecular medicine

... Therefore warming rate is an important consideration in combatting freezing damage (Gao and Critser, 2000;Waters et al., 2020). In particular, a sample that has undergone vitrification may be especially susceptible to ice recrystallization if the warming rate is too slow (Bojic et al., 2021;Zhan et al., 2022). ...

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Applying soft matter techniques to solve challenges in cryopreservation

... Apoptosis results when electrical pulses that are administered to cancer cells provoke thermal damage to internal structures and cell membranes [4]; it is known as inhibition of Fractal Fract. 2025, 9,34 2 of 20 proliferation on biological cells [5]. Healthy cells that are in the surrounding media are less sensitive to applied electrical pulses, avoiding significant damage. ...

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Fractional Electrodamage in A549 Human Lung Cancer Cells

... Since then, the scope of vitrification has broadened to include applications in reproductive medicine and organ preservation [177][178][179][180]. For example, rat livers have been vitrified and demonstrated preserved tissue architecture after thawing [158], and small animal hearts and kidneys have also been successfully vitrified [156,181]. A notable achievement by Fahy's team was the successful vitrification of a rabbit kidney, which functioned for 48 days posttransplant, despite challenges such as persistently elevated creatinine levels and lethargy [182]. ...

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Beyond the icebox: modern strategies in organ preservation for transplantation

... Islets of Langerhans are mini-organs, cryopreserved islets or islets that have been cultured for a longer period, are usually not suitable for islet transplantation 2,4-8 . Although a great progress has been made in the cryopreservation of human islets 8 , in vitro culture of human islets, which will subject islets to stress, is still a necessary and critical step in islet transplantation, so that the quality and quantity of donor islets can be evaluated, and it also provides additional time to get recipients ready for the operation. In vitro culture of islets will also allow researchers to study their function, to characterize the subtype of cells in human islets, and to study the function of pancreatic genes or subtypes of cells. ...

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Multi-omics analysis of long-term cultured human islets

... In recent years, studies on organ and tissue cryopreservation have highlighted the importance of proper cryoprotectant addition. Contemporary cryoprotectants, like M22, VMP and VS55, contain any combination of the most commonly used cryoprotecting agents, ethylene glycol (EG) and DMSO [27], which have been used extensively in cryoprotection of human oocytes and zygotes [28], ovarian tissue [29], rat and rabbit kidneys [30,31], mouse hearts [32], and lamb cartilage [33]. ...

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Controlling Equilibrium Vitrification Using Electrical Impedance Spectroscopy