Zhu Li’s research while affiliated with Southwest University and other places

Chlorophorus Chevrolat, 1863, one of the most species-rich genera of Clytini, comprises 36 subgenera and 302 species/subspecies, with some species being of significant economic importance. To assess the monophyly and subgeneric system of this genus, we newly obtained mitochondrial genomic data from 21 species of Chlorophorus via high-throughput sequencing and reconstructed the phylogeny of this genus using ML and BI methods. The mitochondrial genomes of all sequenced Chlorophorus species were found to comprise 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one non-coding region (control region, CR), reflecting a highly conserved gene arrangement. The mitochondrial genomes of the 21 Chlorophorus species range from 15,387 bp to 15,779 bp in length, and all exhibited AT bias. Analysis of the non-synonymous and synonymous substitution rates revealed that ATP8 has the highest evolution rate, whereas COI has the lowest. Further, phylogenetic analyses were performed based on different datasets (13 PCGs + 2 rRNAs, 13 PCGs + 2 rRNAs + 22 tRNAs, PCG123, PCG12) using maximum likelihood (ML) and Bayesian inference (BI) methods. The results indicate that Chlorophorus is monophyletic, but the subgenera Humeromaculatus Özdikmen, 2011; Immaculatoides Özdikmen, 2022; Brevenotatus Özdikmen, 2022; and Chlorophorus (s. str.) Chevrolat, 1863, are not monophyletic. Based on the comparison of male genitalia, we found that the sclerites of the endophallus may exhibit phylogenetic signals for the genus.

Chlorophorus fainanensis Pic, 1918 is redescribed. Four species, C. coniperda Holzschuh, 1992, C. diversicolor Holzschuh, 2016, C. orbatus Holzschuh, 1991 and C. pinguis Holzschuh, 1992 are newly reported from China. A new synonymy, Chlorophorus arciferus (Chevrolat, 1863) = Chlorophorus semisinuatus Pic, 1949, syn. nov. is proposed.

The complete mitochondrial genome of Nadezhdiella cantori (Hope, 1843) is 16,049 bp in length, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and an A-T rich region (control region). The gene order is conserved and identical to most other previously sequenced Cerambycidae. Phylogenetic analysis showed that the newly sequenced N. cantori was in a well-supported clade tribe Cerambycini, subfamily Cerambycinae. These results support the currently accepted taxonomy and provide a better understanding of the phylogenetic analysis of the Cerambycidae.

Oberea acuta Gressitt, 1951 is redescribed, the diagnosis of O. notata Pic, 1936 and O. bisbipunctata Pic, 1916 were provided based on examination of type material and additional specimens. Illustrations of habitus and genitalia are provided, and a key to separate these three species is presented. Their distribution is critically examined, notably that of O. bisbipunctata, whose previous records in China are invalidated. Oberea shimomurai Kurihara & N. Ohbayashi, 2007 is considered a junior synonym of Oberea notata Pic, 1936.

In the current work, the following taxonomic changes of genus Oberea Dejean, 1835 are proposed: O. flavescens Breuning, 1947, rest. stat.; O. toi Gressitt, 1939, rest. stat.; O. sylvia Pascoe, 1858, rest. stat.; O. taiwana Matsushita, 1933 = O. taihokuensis Breuning, 1962, syn. n.; O. sumbana Breuning, 1961 = O. antennata Franz, 1972, syn. n.; O. brevithorax Gressitt, 1939 is newly recorded from Vietnam.