Zhixiong Lin’s research while affiliated with Guangdong Academy of Agricultural Sciences and other places

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Publications (6)


Figure 4. Total antioxidant activities of the five sweet and five sour wampee varieties obtained by the ORAC assay. Bars with no letters in common are significantly different (p < 0.05).
Figure 5. The cellular activities of the five sweet and five sour wampee varieties obtained by the ORAC assay. Bars with no letters in common are significantly different (p < 0.05).
Titratable acid and total sugar contents of sweet and sour wampee varieties.
Phytochemical components of the sweet and sour wampee varieties (µg/g FW).
Comparative Analysis of Phytochemical Profiles and Antioxidant Activities between Sweet and Sour Wampee (Clausena lansium) Fruits
  • Article
  • Full-text available

April 2022

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65 Reads

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13 Citations

Foods

Xiaoxiao Chang

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Jianping Pan

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Yusheng Lu

As a local medicine and food, wampee fruit, with abundant bioactive compounds, is loved by local residents in Southern China. Titratable acid (TA), total sugar (TS), and total phenolic and flavonoid contents were detected, and phytochemical profiles and cellular antioxidant activities were analyzed by the HPLC and CAA (cellular antioxidant activity) assay in five sweet wampee varieties and five sour wampee varieties. Results showed that the average TS/TA ratio of sweet wampee varieties was 29 times higher than sour wampee varieties, while TA content was 19 times lower than sour wampee varieties. There were much lower levels of total phenolics, flavonoids, and antioxidant activities in sweet wampee varieties than those in sour wampee varieties. Eight phytochemicals were detected in sour wampee varieties, including syringin, rutin, benzoic acid, 2-methoxycinnamic acid, kaempferol, hesperetin, nobiletin, and tangeretin, while just four of them were detected in sweet wampee varieties. Syringin was the only one that was detected in all the sour wampee varieties and was not detected in all sweet wampee varieties. Correlation analysis showed significant positive correlations between TA with phenolics, flavonoids, and total and cellular (PBS wash) antioxidant activities, while there were significant negative correlations between TS/TA with phenolic and cellular (no PBS wash) antioxidant activities. This suggested that the content of titratable acid in wampee fruit might have some relationship with the contents of phenolics and flavonoids. Sour wampee varieties should be paid much attention by breeders for their high phytochemical contents and antioxidant activities for cultivating germplasms with high health care efficacy.

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Transcriptome and Metabolome Analyses Reveal Key Genes potentially relative to sucrose and citrate accumulation in wampee (Clausena Lansium Skeels) Fruit During Fruit Advancement and Ripening

August 2020

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41 Reads

Background: The seedless Clausena lansium Skeels, belonged to the Rutaceae Clausena family, is the main commercial variety in China currently. The high citrate contents in mature fruit have seriously restricted the development of the industry. The dynamic changes of sucrose and citrate, as well as the involved key regulatory genes or metabolism pathways during the fruit development of seedless wampee, however, remain unclear. Results: In this study, we used the seedless wampee fruits at various advancement levels as materials and specified a total of 623 compounds by widely targeted metabolome. Metabolome analysis further revealed that sucrose was gradually accumulated throughout the fruit development, while citrate was accumulated rapidly in the transitionary period from fruit expansion to color conversion, and decreased slowly after color conversion period without significant difference. Moreover, we identified several key differentials expressed genes existed in sucrose as well as citrate metabolism, that mainly encode insoluble acid invertases (IVR), sucrose-phosphate synthase (SPSs), sucrose synthase (SuSy), NAD-dependent malate dehydrogenase (NAD-MDH), trehalose 6-phosphate synthase (TPS), and ATP citrate lyase (ACL), by comparing the transcriptome data of fruit in each stage. Conclusions: We found that sucrose content was mainly regulated by SUS, IVR, SPS, and TPS, the citrate synthesis was mainly regulated by MDH, and the decomposition of citrate was mainly controlled by ACLβ in the acetyl-CoA pathway, suggesting that GABA shunt pathway did not play a decisive role.


Phytochemical profiles of five different chestnut cultivars.
Cellular antioxidant activities of five different chestnut cultivars.
Phytochemical Profiles and Cellular Antioxidant Activities in Chestnut (Castanea mollissima BL.) Kernels of Five Different Cultivars

January 2020

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241 Reads

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17 Citations

Molecules

In this study, the phytochemical profiles, total and cellular antioxidant activities of five different Chinese chestnut (Castanea mollissima BL.) cultivars were analyzed. Phenolics, flavonoids as well as phytochemical compounds in five cultivars of chestnut kernels were determined. Results showed that the free forms played a dominant role in total phenolics, flavonoids and antioxidant activities of all five cultivars of chestnut kernels. The cultivar ‘Fyou’ showed the highest total and free phenolic contents, ‘Heguoyihao’ showed the highest total and free flavonoids contents, and ‘Chushuhong’ showed the highest total and cellular antioxidant activities. Eight phenolic compounds were detected, and chlorogenic acid, gallic acid, and quercetin were shown as three predominant components in all five cultivars. These results provide valuable information which may be a guidance for selection of good chestnut variety to be used as functional food.


Comparative assessment of phytochemical profiles and antioxidant activities in selected five varieties of wampee ( Clausena lansium ) fruits

June 2018

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70 Reads

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30 Citations

Wampee (Clausena lansium) fruit is a good resource for food ingredient because of abundant bioactive compounds. This study investigated phenolics, flavonoids, phytochemical composition and antioxidant activities of five different varieties of wampee fruit. Phytochemical composition was analysed by HPLC, and the antioxidant activities of wampee fruits were detected by ORAC, DPPH, FRAP and PSC assays. Results showed that the variety of ‘YangShanDuHe (YSDH)’ contained the highest contents of total phenolics and flavonoids, and the highest value of three phytochemical compounds: vanillic acid, ferulic acid and rutin. The sweet wampee variety ‘CongChengTianHuangPi (CCTHP)’ showed the lowest values of total phenolics, flavonoids and antioxidant activities. According to the rank of five different varieties by the values of total phenolic and flavonoid contents and antioxidant activities, YSDH was characterised having the highest antioxidant potential, while the least one was CCTHP. These results may provide health guidance for wampee processing and consuming.


Phenolics content in four developmental stages of wampee leaves (mean ± SD, n = 3). Bars with no letters in common are significantly different (p< 0.05).
Flavonoids content in four developmental stages of wampee leaves (mean ± SD, n = 3). Bars with no letters in common are significantly different (p< 0.05).
Total antioxidant activity (ORAC) in four developmental stages of wampee leaves (mean ± SD, n = 3). Bars with no letters in common are significantly different (p< 0.05).
Cellular antioxidant activity (CAA) in four developmental stages of wampee leaves (mean ± SD, n = 3). Bars with no letters in common are significantly different (p< 0.05). A: PBS wash; B: no PBS wash.
Impact of Leaf Development Stages on Polyphenolics Profile and Antioxidant Activity in Clausena lansium (Lour.) Skeels

June 2018

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176 Reads

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22 Citations

Clausena lansium (Lour.) Skeels, commonly known as “wampee,” is an excellent food ingredient of medicinal value. Effects of leaf developmental stages on the composition of phenolics, flavonoids, and antioxidant activity were investigated. Phenolics composition was studied using HPLC-PAD, whereas antioxidant activity was estimated by oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. Increase in bound flavonoids, quercetin, and cellular antioxidant activity was observed in bound and free fractions at different stages of leaf development. Predominantly, quercetin and ferulic acid contents were high in free and bound fractions of old leaves. In addition, phenolic components depicted highly significant positive association ( p < 0.05) with antioxidant activity. Overall, old leaves of wampee have utility value similar to leaf buds, so they could be a more sustainable and economical source of bioactive compounds for commercial application in nutraceutical and pharmaceutical industries.


Effects of 1-MCP Treatment on the Shelf Life of “Yueyinzaocui” Pear

December 2015

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21 Reads

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2 Citations

"Yueyinzaocui" pear (Pyrus pyrifolia cv. "Yueyinzaocui") is a variety with good quality that is cultivated in the northeast area of Guangdong province in China and harvested from late June to early July, but it only has a 1-week shelf life. For the purpose of extending the shelf life of the "Yueyinzaocui" pear, 1μL/L of 1-MCP (methylcyclopropene) was used to treat postharvest fruit. The results showed that fruit firmness continuously decreased; however, the respiration rate, ethylene production, β-GAL (galactosidase) activity and β-GAL transcript level increased during storage at room temperature. Compared with untreated fruit, the decline of fruit firmness during storage was delayed in 1-MCP-treated fruit. Fruit respiration rate was decreased and the ethylene production peak was postponed by 1-MCP treatment. The activity of β-GAL and transcript level of β-GAL gene was reduced, and the shelf life of the "Yueyinzaocui" pear at room temperature was extended for 10 days by 1-MCP treatment. Practical Applications: The purpose of this research is to extend the shelf life of "Yueyinzaocui" pear, and the results of this study showed that 1-MCP (methylcyclopropene) treatment extended its shelf life by 10 days at room temperature. "Yueyinzaocui" pear will rot rapidly in 1 week because of β-galactosidase activity, which caused sale burden for farmers. So if the farmers use the method studied in this research, 1μL/L 1-MCP treatment, it would reduce their pressure and may increase their income.

Citations (4)


... Both the pulp and the pericarp are edible and known for their high nutritional value and distinct flavor [2]. Previous studies have highlighted the presence of bioactive compounds in Wampee, such as phenolics, alkaloids, coumarins, and volatile oils [2][3][4], which exhibit a wide range of biological activities, including anti-inflammatory, antioxidant, neuroprotective, hepatoprotective, and anti-tumor effects [5][6][7][8][9]. Despite its known medicinal properties, the pericarp and seeds of Wampee fruit are often discarded during the processing of the fruit, leading to a significant loss of valuable resources. ...

Reference:

Study on the Chemical Composition and Anti-Tumor Mechanisms of Clausena lansium Fruit By-Products: Based on LC-MS, Network Pharmacology Analysis, and Protein Target Validation
Comparative Analysis of Phytochemical Profiles and Antioxidant Activities between Sweet and Sour Wampee (Clausena lansium) Fruits

Foods

... Of those, Chlorogenic acid was found as a major compound in all the chestnut cultivars extracts with the content in the range of 9.75-12.22 mg/100 g FW [34]. From the involucres parts of C. mollissima extract, 9 major compounds, including 6 flavonoids and 3 polyphenolic acids were isolated and identified [14]. ...

Phytochemical Profiles and Cellular Antioxidant Activities in Chestnut (Castanea mollissima BL.) Kernels of Five Different Cultivars

Molecules

... Skeels), a tropical fruit from the Rutaceae family, is preferably distributed in Southeast Asian countries and southern China [8]. Scholars have determined the abundant bioactive compounds in wampee fruit, such as phenolics, flavonoids, coumarin, and amides, and evaluated their antioxidant, antibacterial, anti-inflammatory, anti-obesity, and anti-tumor activities [9,10]. However, little work concentrated on its by-product seed which is often discarded directly as waste. ...

Comparative assessment of phytochemical profiles and antioxidant activities in selected five varieties of wampee ( Clausena lansium ) fruits
  • Citing Article
  • June 2018

... Wampee is native to the southern regions of China and has a long history of consumption and medicinal use in the country. The fruit of the wampee is rich in sugars, organic acids, lipids, polyphenols, flavonoids, and other compounds, providing a wealth of nutrients for the human body and possessing health benefits and medicinal value [9,49,50]. The nutritional and medicinal components and their content differences in the three tissues of the wampee fruit-peel, pulp, and seed-directly affect the commercial value and development potential of wampee, especially in areas rich in wampee germplasm resources like Yongxing. ...

Impact of Leaf Development Stages on Polyphenolics Profile and Antioxidant Activity in Clausena lansium (Lour.) Skeels