Zhisheng Kan’s research while affiliated with Capital Medical University and other places

Background and Study Aims To explore the usefulness of a unilateral approach for clipping multiple aneurysms including a contralateral paraclinoid aneurysm. Materials and Methods Between January 2011 and May 2016, the unilateral approach was used to clip a contralateral paraclinoid aneurysm plus additional ipsilateral aneurysms. The records of these patients were analyzed retrospectively. Results A total of 27 bilateral aneurysms in 12 patients were clipped successfully via a unilateral pterional craniotomy. All patients showed a good recovery (Glasgow Outcome Scale score: 5). Postoperative digital subtraction angiography or computed tomography angiography did not show any residual aneurysm. Of the three patients with preoperative visual symptoms, two showed improvement. No visual function impairment was observed among the others. Conclusion The contralateral approach remains a safe and reliable treatment option for selected paraclinoid aneurysms and allows to clip additional ipsilateral aneurysm(s). Feasibility depends on the type of paraclinoid aneurysm and other specific related features. It is an alternative approach for experienced neurosurgeons.

Background: Long non-coding RNA (lncRNA) linc01116 was found to be abnormally expressed in many malignant tumor tissues and involved in cancer progression, but its expression and role in glioma tissue is still unclear. This study was designed to investigate the expression of linc01116 in glioma tissues and the role of linc01116 in glioma cell migration and invasion. Methods: Linc01116 and miR-31 expression was measured in 135 cases of human glioma tissues and normal brain tissues using Real-time quantitative PCR (RT-qPCR). The function of linc01116 in glioma cells was determined by Transwell invasion assays and nude mice metastasis assay. Luciferase reporter system was used to confirm the connection between linc01116 and miR-31, or miR-31 and radixin. Results: Linc01116 is highly expressed in glioma tissue and cells, along with low expression of miR-31, and there was a negative correlation between the expression of linc01116 and miR-31 in glioma tissue. In addition, the expression of linc01116 in glioma patients with metastasis was significantly higher than that in patients without metastasis, while miR-31 was significantly lower. In vitro and in vivo studies shown that linc01116 promoted invasion and migration of glioma cells. The luciferase gene reporter system had confirmed that linc01116 targeted miR-31 and miR-31 targeted radixin in U251 cells. Moreover, radixin was downregulated and decreased E-cadherin protein expression, but increased MMP-9 and vimentin protein expression in U251 cells. Conclusion: LncRNA linc01116 is highly expressed in glioma tissues, and it promotes glioma cell migration and invasion by modulation of radixin targeted by miR-31.

Objective: To compare the pterional and frontolateral approach to determine the most effective route for paraclinoid aneurysm surgery. Methods: Between June 2010 and December 2015, a total of 176 patients with paraclinoid aneurysm underwent surgical clipping, 96 through the pterional and 80 through the frontolateral approach. We analyzed the two groups and compared demographic, radiologic, and clinical variables including age, sex, aneurysm type,intraoperative rupture rates, operative time,anatomical obstacles,outcome and postoperative complications. Results: The 2 groups were comparable with respect to baseline characteristics. The mean operation time was also signifcantly shorter in frontolateral group than in the pterion group (204.3 min vs. 264.1 min, p < 0.05). Furthermore, the mean craniotomy area was much smaller in the frontolateral group (1255.4 mm2vs. 2758.5 mm2, p < 0.05). No patient experienced rebleeding in either group. In the frontolateral group, the exploration allows enough room for intracranial manipulation with maximal protection of the brain and other intracranial structures. Conclusion: The frontolateral approach is a simple, reliable, and efficient procedure. The frontolateral approach could be a good alternative to the classic pterional approach for the treatment of paraclinoid aneurysms.

Failure to properly repair damaged due to myocardial infarction is a major cause of heart failure. In contrast to adult mammals, zebrafish hearts show remarkable regenerative capabilities after substantial damage. To characterize protein dynamics during heart regeneration, we employed an HPLC-ESI-MS/MS approach. Myocardium tissues were taken from sham operated fish and ventricle resected sample at three different time points (2 days, 7 days, and 14 days), dynamics of protein expression were analyzed by an ion current-based quantitative platform. More than two thousand protein groups were quantified in all 16 experiments. Two hundred and nine heart regeneration related protein groups were quantified and clustered into 6 time-course patterns. Functional analysis indicated that multiple molecular function and metabolic pathways were involved in heart regeneration. Interestingly, Ingenuity Pathway analysis revealed that P53 signaling was inhibited during the heart regeneration, which was further verified by Q-PCR. In summary, we applied systematic proteomics analysis on regenerating zebrafish heart, uncovered the dynamics of regenerative genes expression and regulatory pathways, provided invaluable insight to design regenerative-based strategies in human hearts.

Background The clipping of multiple intracranial aneurysms in 1 stage is uncommon. In this case, we report clipping of an ophthalmic aneurysm associated with multiple anterior and posterior circulation aneurysms via the Dolenc approach. Methods The main symptoms of the patient are headache, along with nausea and vomiting. The patient's arteriogram revealed a wide-necked aneurysm of the right ophthalmic artery, an irregular aneurysm of the anterior communicating artery, and a basilar artery aneurysm. The surgical intervention for these aneurysms is a challenge because of the complex anatomical relationship with the surrounding structures. The 3 aneurysms, which were not amenable to a single intervention, were successfully clipped in 1 incision. Results After surgery, the patient reported feeling well. One year after surgery, the patient had no SAH recurrence. Conclusions Occasionally, surgical treatment was used even for aneurysms of the carotid-ophthalmic artery with aneurysms of anterior communicating artery and basilar artery, which are contraindicated for interventional therapy.