Zahraa K Zedan’s scientific contributions

What is this page?


This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.

Publications (5)


Generation of hepatocyte from mouse mesenchymal stem cells in Vitro Council for Innovative Research
  • Article
  • Full-text available

May 2020

·

44 Reads

·

Zahraa K Zedan

·

Shalal M Hussain

·

This study was designed to generate hepatocyte cells from mesenchymal stem cells (MSCs) isolated from mouse bone marrow via using culturing protocols for this goal. Cells of MSCs were isolated by collecting the thigh bone of 50 albino mice, both femur and tibia were collected and cells were flushed from bones and MSCs have been isolated based on the ability of MSCs to adhere on plastic surfaces. MSCs reactivity to CD45, CD90, CD34 and CD105 were tested by immunocytochemistry. Isolated MSCs exhibited positive reactivity towars CD105 and CD90 cell surface markers. MSCs were negative for the hematopoietic surface markers including CD34 and CD45. Differentiation into hepatocyte was induced by adding hepatogenic specific growth factors (Hepatocytes growth factors (HGF), Fibroblast growth factor4 (FGF4), oncostatin M and dexamethasone) to the differentiation medium. Cells were examined after differentiation protocol and characterized using immunocytochemistry analysis for Albumin and alpha fetoprotein. Positive results were observed indicating the potential ability of the isolated bone marrow MSCs to differentiate into Hepatocytes.

Download

Figure (2): Immunocytochemistry analysis of isolated BM-MSCs: shows the positive reaction of cells towards CD105 and CD90 markers of cell surface and the negative reaction for CD34 and CD45 cell surface markers.
Figure (4): Immunocytochemical analysis of cytochrome oxidase p450 (CYP3A44 p450) in hepatocytes like cells. Positive reaction in (A) in induced hepatocytes like cells, while (B) shows the negative reaction of cells against mouse antihuman CYP3A4 p450. Elisa assay of CYP3A4 in differentiated cells lysate Results revealed that a significant increase in CYT3A4 concentration in hepatocyte lysate at 21 day (65.27± 0.52) ng/ml in comparison with 14 and 7 33.0 ±0.0.72 and 16.73±0.92) ng/ml respectively (Figure 5).
Identification of Cytochrmoe oxidase p450 in Heptocytes generated from in vitro differentiation of mouse mesenchymal stem cells Council for Innovative Research

May 2020

·

72 Reads

This study was designed to identify the presence of cytochrome oxidase p450 (CYP3A4), in the hepatic cell lines which was generated from the in vitro differentiation of mouse bone marrow mesenchymal stem cells. Bone marrow meenchymal stem cells were first isolated by collecting both the femur and tibia of the mouse thigh bone, then exess tissue were removed and the cells were flushed from the bones and cultured under highly aseptic conditions. The isolated cells were characterized as a mesenchymal stem cells via using immunocytochemistry analysis. The characterized hepatocytes were subjected to a differentiation protocol in which the differentiation medium was contained a spesfic growth factor including (Hepatocytes growth factor (HGF), Fibroblast growth factor4 (FGF4), Oncostatin M and Dexamethasone, All theses growth factor was used for directing the BM-MSCs twards the hepatic line of cells. After three weeks of the differentiation protocol the diffrentation cells were examined for the presence of Cytochrome oxidase p450 (CYP3A4), which consider as one of the specific markers of hepatocytes. Identification eas done via two different methods first, via using the immunocytochemical analysis for the antihuman mouse CYP3A4 antibody and second by ELIZA test. The results showed a posotive reactivity of the differentiated cells towards the mouse antihuman CYP3A4 antibody as well as the eliza results showed a significant increase in the enzyme level of the cell lysate during the weeks of the differentiation experiment.


Immune Effect of Viable and Heat Killed Lactobacillus acidophilus in Mice Infected with Salmonella typhimurium

December 2012

·

21 Reads

·

2 Citations

Journal of Al-Nahrain University-Science

This study was designed to evaluate the immunological effect of viable and heat killed Lactobacillus acidophilus in mice infected with Salmonella typhimurium. The immunological parameters were total and absolute counts of leucocytes and phagocytic index of peritoneal cells. Twelve mice were divided into four groups. Each group consists of 3 mice. Group 1 was used as a negative control. Group 2 was infected with Salmonella typhimurium and use as positive control. Group 3 was fed with viable Lactobacillus acidophilus culture, and infected with culture of Salmonella typhimurium. Group4 was fed with heat killed Lactobacillus acidophilus culture, and infected with Salmonella typhimurium. Results indicated that treatment mice with viable Lactobacillus acidophilus were effective in enhancing the immune responses against Salmonella typhimurium as compared to negative (distilled water) and positive (Salmonella typhimurium) controls. While, mice fed with heat killed Lactobacillus acidophilus showed no enhancing immune effect on mice infected with Salmonella typhimurium.


Hepatoprotective Effect of Lactobacillus Plantarum Against Salmonella Typhimurium in Mice

December 2011

·

6 Reads

·

9 Citations

Journal of Al-Nahrain University-Science

This study was designed to investigate the ability of Lactobacillus plantarum as hepatoprotective microorganism against Salmonella typhimurium in mice. Twelve albino mice were divided into 4 groups designated as CC, PC, SC and PS. Each group consisted of 3 mice, and was subjected to the following treatments; CC; this group was used as a control. PC; this group was dosed with 0.1ml of 10 9 cfu/ml Lb plantarum culture.SC; this group was dosed with 0.1ml. of 2.5×10 7 cfu/ml Sal. typhimurium culture. PS; this group was dosed with 0.1ml of 10 9 cfu/ml Lb. plantarum culture, and infected with 0.1ml of 2.5×10 7 cfu/ml culture of Sal. typhimurium, then serum levels of Glutamic Pyruvic Transaminase (GPT) and Glutamic Oxaloacetic Transaminase (GOT) of treated mice were measured and histological sections were made from liver to evaluate protective effect of Lb. plantarum. Results showed that mice treated with Lb plantarum exhibited a significant (p≤ 0.05) decrease in serum level of GPT and GOT (43.25 U/L and 95.50 U/L, respectively) in comparison with their levels in serum of control group. Mice infected with Sal. typhimurium showed a significant (p≤ 0.05) increase in serum level of GPT (87.3 U/L), and a significant increase (p≤0.05) in serum level of GOT (227 U/L) in comparison with their levels (55.23 U/L and 113 U/L) in control group. Mice treated with Lb. plantarum and infected with Sal. typhimurium showed a significant (p≤ 0.05) decrease in serum GPT level (47.5U/L) and significant decrease (P≤0.05) in serum GOT level (121 U/L) in comparison with their levels in mice infected with Sa l. typhimurium. Histopathological study showed that infection with Sal. typhimurium caused a necrosis, degenerative changes and inflammatory cells infiltration as compared with control while, treatment with Lb plantarum prevented the histopathological effect of Sal. typhimurium on mice liver.


Protective effect of Lactobacillus plantarum against Salmonella typhimurium in mice Protective effect of Lactobacillus plantarum against Salmonella typhimurium in mice

January 2010

·

24 Reads

·

2 Citations

Journal of the Faculty of Medicine Baghdad

Background: Probiotics are defined as living microbial food supplements that beneficially affect the host by improving its intestinal balance. In recent years there is a great interest for viable microorganisms that promote or support a beneficial balance of microbial population of gastrointestinal tract. Probiotics may be consumed in different forms, comprising foods, mainly in fermented state and pharmaceutical products, mainly as capsules or in microencapsulated forms.Methods: Twelve albino mice were divided into four groups designated as 1, 2, 3 and 4. Each group consists of 3 mice, and subject to the followings treatments: Group 1: This group was used as a control. Group 2: This group was dosed with 0.1ml. of 0.5 × 105 cfu/ml of Lactobacillus plantarum culture. Group 3: This group was dosed with 0.1ml. of 0.5 × 105 cfu/ml Salmonella typhimurium culture. Group 4: this group was dosed with 0.1ml of 109cfu/ml Lactobacillus plantarum culture, and infected with 0.1ml of 0.5 × 105cfu/ml culture of Salmonella typhimurium, then histological sections were made from intestine and examined to evaluate protective effect of Lactobacillus plantarum.Results: Histopathological study exhibited that intestinal sections of mice infected with Salmonella typhimurium showed a necrosis, degenerative changes and inflammatory cells infiltration as compared with normal sections taken from uninfected mice, while treating with Lactobacillus plantarum prevented the histopathological effect of Salmonella typhimurium on mice intestine.Conclusion: Probiotic L. plantarum had a protective effect on intestine sections of mice infected with Salmonella typhimurium

Citations (1)


... Both infiltration of inflammatory cells and congestion were found , they are agree with Zghair (2012) who demonstrated congestion of blood vessels, infiltration of inflammatory cells, infiltration of kupffer cells and other damages of internal organs after mice inoculation with bacteria (Salmonella). Zedan (2011) mentioned to degeneration, necrosis and infiltration of inflammatory cells were histological damages observed in experimentally infection with Salmonella typhimurium. A previous another study by Malik et al (2015) was confirm the histological lesions in liver of Salmonella infection such as necrosis, degeneration of hepatocytes and inflammation. ...

Reference:

Negative Effects on Some Internal Organs of Rats Infected by Salmonella typhimurium
Hepatoprotective Effect of Lactobacillus Plantarum Against Salmonella Typhimurium in Mice

Journal of Al-Nahrain University-Science