Z.-Z. Wang’s research while affiliated with State Key Laboratory of Medical Genetics of China and other places

Objective: To study the main constituents from Gardenia jasminoides. Method In this study, the chemical constituents of enrichment fraction of iridoid glycosides were isolated by various chromatographic techniques and their structures were elucidated by spectroscopic analyses and comparison of NMR data with those reported in literatures. Results: The 60% ethanol extract of G. jasminoides was subjected to HP-20 macroporous adsorption resin CC to yield 30% ethanol fraction (GJ-2, UPLC-Q/TOF-MS method was used to identify the enrichment fraction of iridoid glycosides). Thirty-one compounds were obtained and characterized as 2'-O-trans-coumaroylgardoside (1), 6'-O-trans-sinapoylgardoside (2), 7-deoxygardoside (3), tarenninoside C (4), 2'-O-coumaroylmussaenosidic acid (5), 10-O-caffeoyl deacetyldaphylloside (6), 6'-O-trans-sinapoylgeniposide (7), genipin-1-O-β-D-gentiobioside (8), geniposide (9), 7-deoxy-8-epiloganicacid (10), secologanoside (11), gardenamide A (12), 6'-O-trans-sinapoyljasminoside B (13), epijasminoside A (14), jasminodiol (15), 6'-O-trans-sinapoyljasminoside L (16), 3-(β-D- glucopyranosyl-oxymethyl)-2,4,4-trimethyl-2-cyclohexen-1-one (17), jasminoside C (18), sinapinic acid (19), caffeic acid (20), methyl gallate (21), C-veratroylglucol (22), β-hydroxypropiosyringone (23), 3-hydroxy-1-(4-hydroxy-3-methoxyphenoyl) propan-1-one (24), threo-guaiacylglycerol-8'-vanillic acid ether (25), 1,2-bis-(4-hydroxy-3-methoxyphenyl)-1,3-propanediol (26), trans-2,3,5,4'-tetrahydroxystilbene-2-β-D-glucoside (27), 1-sinapoyl-β-D-glucopyranoside (28), 1,3,5-trimethoxybenzene (29), rutin (30), and glycyrrhisoflavone (31), respectively. Conclusion: Compounds 1-12are iridoid glycosides and 13-18are monoterpenoid glycosides. Compounds 6, 10, 22-29, and 31 were identified from Gardeniae Fructus for the first time. © 2020, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective: To investigate the major active components and potential molecular mechanism of Qiwei Tongbi Oral Liquid in treatment of rheumatoid arthritis. Methods: After protein targets related with rheumatoid arthritis (RA) were collected by mining literature, DrugBank, TTD and OMIM database, the potentional protein targets based on molecular docking were projected into KEGG databases to illustrate the molecular mechanism of Qiwei Tongbi Oral Liquid. Then RAW264.7 cells induced by LPS were employed to test the predict results. Results: Data analysis showed that 107 potential components acted on 116 RA related targets and 237 pathways. Herb-compound-target-pathway network analysis showed that triterpenoid, flavonoids, sterols and alkaloids isolated from Qiwei Tongbi Oral Liquid were the main active ingredients. These compounds could interact with a group of targets and pathways that might participate in anti-inflammatory, analgesic, immune regulation, proliferation, apoptosis, migration and invasion of synovial fibroblasts, osteoclast differentiation, migration and bone resorption. Some compounds against anti-inflammatory activity were verified by in vitro. Conclusion: The active components of Qiwei Tongbi Oral Liquid could regulate multiple biological pathways by acting with multiple target proteins, playing a role in reducing inflammation and swelling of joints, preventing and reducing the destruction of joint bones, and promoting the repair of damaged joint bones. The research results not only reveal its molecular mechanism and pharmacodynamic components, but also provide a theoretical basis for the subsequent experimental research of Qiwei Tongbi Oral Liquid. © 2020, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

As a commonly used Chinese materia medica, Artemisia annua mainly contains sesquiterpenoids, diterpenes, phenylpropionic acids, coumarins, flavonoids, volatile oil, and other chemical compositions. Its pharmacological activities are anti-malaria, anti-tumor, anti-microbial, anti-parasitic, antipyretic, anti-inflammatory, immunoregulation and so on. The significantly anti-malarial activity has led to its earlier use in the treatment of malaria. In this paper, the chemical constituents and pharmacological activities of A. annua in recent years are reviewed in order to provide a reference for the further development and rational utilization of this plant resource. © 2019, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Chemical constituents from the ethanol extract of Radix Angelicae Pubescentis was isolated and purified through Diaion HP-20 macroporous, silica gel column chromatography, gel filtration over Sephadex LH-20 and preparative HPLC. Two new sesquiterpenoid derivatives were identified as angesesquid A (1) and angesesquid B (2), and their structures were determined. In vitro degeneration model of primary rat disc chondro-cytes was used to evaluate the anti-inflammatory activity of these two compounds. The results showed that compounds 1 and 2 had no anti-proliferation effect. Both compounds inhibited the release of NO, but had no inhibitory activity for the release of PGE 2 . This finding implies that both of these two new sesquiterpenoids could moderately inhibit the inflammatory reaction to some extent. I

Objective To establish the multi-wavelength fusion HPLC fingerprints of Gardeniae Fructus and to make quantitative analysis of the common peaks by ESI-Q-TOF MS. Methods The Kromasil 100-5 C 18 column was used with a mobile phase of acetonitrile-0.1% phosphoric acid in gradient elution; The flow rate was 1.0 mL/min; The column temperature was 35 ℃; The detection wavelength was 238, 327, and 440 nm. Matlab7.1 was adopted for multi-wavelength fusion of the data in CSV format. Results Twenty chemical constituents were detected in fusion fingerprint, which included more information. Sixteen chemical constituents were compared with reference standards and identified by MS. The similarity of 10 batches of Gardeniae Fructus was over 0.90. Conclusion The systematic quantified fingerprint method based on the technique of multi-wavelength fusion fingerprint and identifying 16 constituents can be used for the fully quality control of Gardeniae Fructus. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective To investigate the effect of Reduning Injection on Vero cells and suckling mice infected by EV71 virus and its mechanism in vitro and in vivo. Methods The anti-virus ability was exhibited in Vero cells which were infected by 33 TCID50 and 25 TCID50 titers of EV71 in vitro. Meanwhile, the antiviral activity in vivo was evaluated on suckling mice model, in which suckling mice were intraperitoneal injected with EV71 at 100 μL. After 2 h infection, Reduning Injection 2.60, 1.30, and 0.65 g/kg and ribavirin 10 mg/kg were followed for treatment. To further understand the antiviral effects of Reduning Injection, weigh of suckling mice, clinical symptoms, expression of IL-1β, IL-6, MCP-1, and TNF-α in skeletal muscle were recorded. Moreover, histological examination of skeletal muscle was examined. Results The results demonstrated that Reduning Injection 5.0 mg/mL could obviously inhibit Vero cells lesion induced by EV71 in vitro. Compared with the model group, suckling mouse model results showed the high and middle dose of Reduning Injection could reduce clinical manifestations scores, increase the weight and improve living conditions of model mice, decrease the expression of IL-1β, IL-6, TNF-α, and MCP-1 in skeletal muscle of suckling mice (P<0.05, 0.01). Histological damages in skeletal muscle were also alleviated with Reduning Injection treatment. Conclusion In a suckling mouse model, Reduning Injection can reduce mortality and obviously prolong the survival time of suckling mice caused by EV71 infection. It can alleviate clinical symptoms and growth inhibition caused by the EV71 virus. Inhibition of IL-1β, IL-6, TNF-α, and MCP-1 levels may be the important mechanism of Reduning Injection in the treatment of hand-foot-and-mouth disease. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective: To establish the model for evaluating the quality of Lonicera japonica in antimicrobial activity. Methods :The active database was established on the basis of antimicrobial activity of 21 batches of L. japonica by using the agar drifting diffusion bioassay. Similarly, the chemical composition database was obtained by detecting the fingerprints of 21 batches of L. japonica from HPLC. The correlation between the activity and chemical composition was analyzed by partial least squares (PLS) method. Then, the YZL interval values were determined to evaluate the quality of L. japonica by normal fitting and probability calculation method. The results were verified by the other 10 batches of L. japonica. Results: Fourteen common peaks were obtained from the fingerprints of 21 batches of L. japonica. The standardized regression coefficients of each common peak were got by establishing regression model of the relationship between peak area and activity by PLS method. After normal fitting and defining YZL values, L. japonica was decided as high quality at YZL value ≥ 2.53, on the contrary, as poor quality at YZL value ≤ 1.79. The results were verified by 10 batches of L. japonica. Conclusion: The YZL values could provide a repeatable evaluation methodology for the quality control of L. japonica in antimicrobial activity. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective To investigate the anti-inflammatory constituents from the ethanol extract of Yaobitong Capsule. Methods The chemical constituents were isolated by repeated silica gel column, Sephadex LH-20, and preparative HPLC. The structures were identified by various spectral data analysis and physicochemical properties. The anti-inflammatory effects were evaluated by in vitro model of LPS-stimulated rat thoracic aortic smooth muscle cells (A7r5). Results Eleven compounds were isolated from the contents of the capsule, including 2 organic acids, 1 alkaloid, 2 senkyunolides, 1 sesquiterpene, and 5 coumarins, which identified as ethyl caffeate (1), ferulic acid (2), corydaline (3), senkyunolide H (4), senkyunolide I (5), ligustiphenol (6), columbianetin (7), ulopterol (8), meranzin hydrate (9), angelitriol (10), and 6-[1(R),2(R)-1,2,3-thihydroxy-3-methylbutyl]-7-methoxycoumarin (11). Compounds 1-6 showed the anti-inflammatory activity in different degrees. Conclusion Compounds 1, 3, 6, and 8-11 are isolated from Yaobitong Capsule for the first time, and organic acids and senkyunolide compounds may be the anti-inflammatory pharmacodynamic material bases of Yaobitong Capsule. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective To investigate the chemical constituents from the ethanol extract of Yaobitong Capsule. Methods The chemical constituents were isolated by repeated silica gel column, dynamic axial compression chromatography, Sephadex LH-20 column, and prep-HPLC. Their structures were identified on the basis of NMR spectral data analysis. Results Sixteen compounds were isolated and identified to be nodakenetin (1), columbianetin (2), ferulic acid (3), umbelliferone (4), bergaptol (5), xanthotoxin (6), isoimperatorin (7), scoparone (8), (Z)-6-hydroxy-7-methoxydihydroligustilide (9), 4-hydroxy-3-butylphthalide (10), 3-(3′-hydroxy)- butylphthalide (11), senkyunolide C (12), senkyunolide H (13), senkyunolide I (14), fraxidin (15), and isofraxidin (16). Conclusion Compounds 1—16 are isolated from Yaobitong Capsule for the first time, and compound 11 is obtained by the means of separation for the first time. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective: To compare the neuroprotective effect of different elution fractions and compounds of Epimedium brevicormon on SH-SY5Y cells injury induced by Aβ25-35 so as to screen pharmacologically fraction and compounds. Methods: The isolation and purification of extract from E. brevicormon were performed on D101 macroporous resin column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative liquid chromatography. Hoechst 33342/PI double staining method was used to screen neuroprotective fractions and components. Results: Compared to model group, all fractions and kaempferol-3-O-[α-L-rhamnopyranosyl (1→6)-β-D-galactoside]-7-O-α-L-rhamnopyranoside, icariin, epimedin A, and epimedin B from E. brevicormon could significantly increase the cells viability, except of 95% ethanol fraction. Conclusion: E. brevicormon show some protective effect against Aβ25-35 induced SH-SY5Y cells injury. The CH2CI2-MeOH (5:1) extract from E. brevicormon has the most pharmacologically activity, followed by 30% ethanol and 50% ethanol extracts from E. brevicormon. © 2017, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.