Z B Wang's research while affiliated with Chinese Academy of Sciences and other places

Publications (4)

Article
Protein phosphatase 4 (PPP4) is a protein phosphatase that, although highly expressed in the testis, currently has an unclear physiological role in this tissue. Here, we show that deletion of PPP4 catalytic subunit gene Ppp4c in the mouse causes male-specific infertility. Loss of PPP4C, when assessed by light microscopy, did not obviously affect ma...
Article
Full-text available
Rab family members play important roles in membrane trafficking, cell growth, and differentiation. Almost all components of the cell endomembrane system, the nucleus, and the plasma membrane are closely related to RAB proteins. In this study, we investigated the distribution and functions of three members of the Rab family, Rab3A, Rab27A, and Rab35...
Article
DNA damage has recently been shown to inhibit or delay germinal vesicle breakdown (GVBD) in mouse oocytes, but once meiosis resumes, DNA-damaged oocytes are able to extrude the first polar body. In this study, using porcine oocytes, we showed that DNA damage did not affect GVBD, but inhibited the final stages of maturation, as indicated by failure...
Article
Polar body emission is a specialized cell division throughout the animal kingdom, serving to reduce chromosome ploidy while preserving the egg cytoplasm. Critical to polar body emission are the asymmetric positioning of the meiotic spindle prior to anaphase, with one pole attached to the oocyte cortex, and the simultaneous membrane protrusion durin...

Citations

... Interestingly, PPP4R3C is preferentially expressed in testis in cattle, human and mice [34][35][36], and has been classified as male reproductive tract-specific genes by Roberston et al. [37]. Deficiency of PPP4C results in oligoasthenoteratospermia and sperm tail bending in mice [38]. These elements make PPP43C an interesting candidate gene and the association would confirm the importance of phosphorylation of repair proteins in the DSB repair pathway [33,39]. ...
... RAB3A, with evolutionarily conserved proteins, is essential regulator of membrane tra cking [47]. Researches have found that RAB3A is indispensable in human sperm acrosome reactions and may regulate the quality of oocytes [48,49]. Phospholipase C beta 3 (PLCB3) was considered to be a candidate gene for litter size in Finnsheep, and playing key roles in the development of folliculogenesis and LH signaling [50]. ...
... Strikingly, lower levels of damage, induced by low doses of etoposide, neocarzinostatin or bleomycin, have no or minimal effect on meiotic resumption and maturation [17][18][19][20]; mouse and human oocytes resume meiosis and undergo GVBD. Furthermore, in porcine oocytes, etoposide and bleomycin caused DSBs that did not inhibit GVBD [21,22]. This inability to establish a G2/prophase arrest is primarily attributed to the insufficient activation of the ATM/Chk1 pathway [18,21]. ...
... Manually defolliculated oocytes were injected with mRNA encoding various probes, as described in our previous publications (Leblanc et al., 2011;Shao et al., 2012;Zhang et al., 2008). For sfGFP-ER-3 and GFP-IP 3 R1 plasmids, which lack viral (such as SP6 and T7) promoters for in vitro transcription, purified plasmids were injected into the oocyte germinal vesicle (~3nL of 1mg/mL DNA per oocyte). ...