Yoshio Tsuda's research while affiliated with The National Institute of Infectious Diseases and other places
What is this page?
This page lists the scientific contributions of an author, who either does not have a ResearchGate profile, or has not yet added these contributions to their profile.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
Publications (315)
Mosquito collections using dry-ice traps were conducted at Izumo, Shimane, Japan, from May to October 2008. Trap sites with different surrounding environments (reservoir, riverside vegetation, a shrine in rice fields) and a breeding colony of heron were selected. A total of 4,890 mosquitoes of 16 species in 6 genera were collected by dry-ice traps....
[This corrects the article DOI: 10.1371/journal.pntd.0008986.].
The Asian tiger mosquito ( Aedes albopictus ) arrived in the USA in the 1980’s and rapidly spread throughout eastern USA within a decade. The predicted northern edge of its overwintering distribution on the East Coast of the USA roughly falls across New York, Connecticut, and Massachusetts, where the species has been recorded as early as 2000. It i...
Twenty Japanese mattresses with various periods of use were collected from 11 localities in Japan in 2014. Samples of the mattresses including the padding were removed at 5 collection points in each mattress. The samples were divided into 3 layers (upper, middle, and bottom layers), and the fauna and density of house-dust mites were examined. A tot...
Avian malaria is a mosquito-borne disease of birds caused by avian Plasmodium spp. in worldwide scale. Some naïve birds show serious symptoms which can result in death. Surveillance of vectors and parasites are important to understand and control this disease. Although avian malaria has been found in Japan, detailed prevalence and dynamics remained...
The Culex vishnui subgroups, particularly Culex tritaeniorhynchus, are considered the primary vectors of the Japanese encephalitis virus (JEV) in Asia. Recent molecular phylogenetic analyses of JEV isolates from Asian countries have shown that JEVs with diverse genetic variants are present in Asia. Furthermore, some JEV strains have been found to h...
Vector surveillance was conducted at Narita International Airport in 2021. A total of 8,120 adult mosquitoes were collected. The most abundant species was Culex tritaeniorhynchus (7,821 adults, 96% of the total). The second was Cx. pipiens complex (184 adults) and the third was Aedes albopictus (84 adults). Among them, 8,097 female adults were test...
The efficiency of box traps was evaluated by release-capture laboratory experiments of Culex pipiens form molestus. The basic box trap (13L×18W×10H cm) was composed of three parts, a shading-plate, a top board, and a collection box. The shading-plate was attached to the end of the top board at a 45-degree angle. Under the shading-plate there was a...
Thirty years have passed since the last surveillance of the mosquito fauna on the entire Chichi-jima, Ogasawara Islands, Japan. Herein, we report the results of a mosquito study conducted on the Ogasawara Islands in 2015 and 2017. In this study, 2,371 individuals belonging to seven species in three genera were collected, including 262 mosquitoes be...
Background
In Hokkaido, northern island of Japan, at least seven cases of falciparum malaria were reported by 1951. A survey conducted at that time was unsuccessful in implicating any mosquito species as the possible vector. Although active anopheline mosquito surveillance continued until the middle of the 1980s, there is very limited information o...
The preference of ovipositing Megaselia scalaris for dead adult cockroaches was examined experimentally using the following 6 species: Blattella germanica, Periplaneta fuliginosa, P. brunnea, P. japonica, P. americana, and Blatta orientalis. Five female and 1 male M. scalaris were released into an experimental cage, and exposed to dead adult cockro...
We report the results of mosquito inspection on international aircraft and in government-run areas by the Japanese Quarantine Station, Ministry of Health, Labour and Welfare between 2008 and 2018. In total, 22,452 aircraft were inspected and 822 adult mosquitoes (including 145 dead mosquitoes) were collected from 240 (1.07%). The majority of the mo...
Background: In Hokkaido, northern island of Japan, at least seven cases of falciparum malaria were reported by 1951. A survey conducted at that time was unsuccessful in implicating any mosquito species as the possible vector. Although active anopheline mosquito surveillance continued until the middle of the 1980s, there is very limited information...
Background: After World War II in Hokkaido, northern island of Japan, at least seven cases of falciparum malaria were reported by 1951. A survey conducted at that time was unsuccessful in implicating any mosquito species as the possible vector. Although active anopheline mosquito surveillance continued until the middle of the 1980s, there is very l...
Background
Captive populations of penguins outside of their natural distributions are often maintained in outdoor facilities, such as zoos and aquariums. Consequently, such penguins in captivity are constantly exposed to mosquito vectors and risk of avian malarial infection during their active period from spring to autumn, which can be lethal to th...
Japanese encephalitis virus (JEV) is maintained in an enzootic cycle between swine, water birds, and mosquitoes. JEV has circulated indigenously in Asia, with Culex tritaeniorhynchus as the primary vector. In some areas where the primary vector is scarce or absent, sporadic cases of Japanese encephalitis have been reported, with Aedes japonicus jap...
Nagano Prefecture, an inland mountain area of Japan, extends widely north and south, with elevation that varies greatly by location. Mosquitoes transmitting infectious disease have a diversity of habitats in Nagano, and many species can be expected there. However, there have been few reports about mosquito fauna in this region; in particular, littl...
Tabanid flies (Tabanidae: Diptera) are common hematophagous insects known to transmit some pathogens mechanically or biologically to animals; they are widely distributed throughout the world. However, no tabanid-borne viruses, except mechanically transmitted viruses, have been reported to date. In this study, we conducted RNA virome analysis of sev...
The development of sequencing technologies, in recent years, gives novel insights into the diversity of viruses in arthropods. Human pathogenic or possible pathogenic arthropod-borne viruses (arboviruses) including novel viruses from mosquitoes and ticks have been found by RNA virome analysis using a high-throughput sequencer. However, virome studi...
The introduction of exotic disease vectors into a new habitat can drastically change the local epidemiological situation. During 2012–2015, larvae and an adult of the yellow-fever mosquito, Aedes aegypti, were captured alive at two international airports serving the Greater Tokyo Area, Japan. Because this species does not naturally distribute in th...
Nagano and Yamanashi prefectures of Japan comprise an inland mountain area that extends widely north and south, with elevation varying greatly by location. Mosquitoes transmitting infectious disease have a diversity of habitats in Nagano and Yamanashi, and many species can be expected there. However, there have been few reports on mosquito fauna; i...
Japanese encephalitis (JE) remains a public health concern in several countries, and the Culex mosquito plays a central role in its transmission cycle. Culex mosquitoes harbor a wide range of viruses, including insect-specific viruses (ISVs), and can transmit a variety of arthropod-borne viruses (arboviruses) that cause human and animal diseases. T...
Cemeteries are suitable habitats for Aedes albopictus. The dispersal of adults emerging at a cemetery was studied by a mark-release-recapture experiment during the period July 11–15, 2016 in Okayama, Japan. Larvae and pupae of Ae. albopictus were collected from artificial containers found in the cemetery, reared to the adult stage, and used for the...
IntroductionAedes albopictus (Skuse) is an important vector of arboviral diseases, including dengue, chikungunya and Zika virus disease. Monitoring insecticide resistance and mechanisms by which the mosquito develops resistance is crucial to minimise disease transmission.AimTo determine insecticide resistance status and mechanisms in Ae. albopictus...
We found a species of Lutzia Theobald (1903) (Diptera: Culicidae) in Chiang Mai Province and other provinces in northern Thailand which bears morphological and DNA sequence differences from the three species of Lutzia, subgenus Metalutzia Tanaka, previously recorded from Thailand, namely Lt. fuscana (Wiedemann), Lt. halifaxii (Theobald) and Lt. vor...
Mosquito collection was carried out monthly using dry-ice traps at Yonago Waterbirds Sanctuary, Tottori Prefecture, Japan, and Culex inatomii was found to be present at a high density. One gynandromorph specimen of Cx. inatomii was collected in July 2017 and the morphology of the head, abdomen, and anterior claws of the foretarsi and midtarsi was e...
In 2014 in Japan, 162 autochthonous dengue cases were reported for the first time in nearly 70 years. Here, we report the results of the detection and isolation of dengue virus (DENV) from mosquitoes collected in Tokyo Metropolis in 2014 and 2015. The phylogenetic relationship among DENV isolates from mosquitoes and from patients based on both the...
Avian malaria parasites of wild birds and their vector mosquitoes have been studied intensively in recent years mainly because of the development of molecular techniques to detect and identify genetic lineage of parasites. This review focused on field studies of vector mosquitoes of avian malaria parasites and examined geographic distributions of v...
Culex (Culiciomyia) spiculothorax was described from Thailand based on the presence of spiculation on the thorax of larvae. Adult females are characterized but are indistinguishable from those of related species, such as Cx. pallidothorax. Phylogenetic analysis of mitochondrial oxidase subunit I (COI) sequences revealed that specimens identified as...
Getah virus (GETV; genus Alphavirus, family Togaviridae) is a mosquito-borne virus known to cause disease in horses and pigs. In 2014, for the first time in ∼30 years, a sudden GETV outbreak occurred among racehorses in Ibaraki, Japan. Two years before this outbreak, we obtained multiple GETV isolates from Culex tritaeniorhynchus mosquitoes collect...
Japanese encephalitis virus (JEV) is the most frequent cause of mosquito-borne encephalitis in Asian countries. Several culicine species are potential vectors. The primary JEV vectors feed mainly on cows (a dead-end host for JEV), pigs (an amplifying host), and, occasionally, humans (a dead-end host). It is essential to determine blood-feeding patt...
A total of 512 mosquito specimens, collected in 2013 and 2014 from 18 study sites from Hokkaido to Kagoshima, Japan, were analyzed for DNA barcoding based on the nucleotide sequence of cytochrome c oxidase subunit I (COI) region, following which the gene sequences of 240 mosquitoes from 45 species in 11 genera were registered in GenBank. Aedes mosq...
The mosquito, Aedes aegypti (L.) originated in Sub-Saharan Africa as a dark form sylvan species (A. aegypti formosus). Evolution of A. aegypti aegypti type form as a human commensal facilitated its colonization of most semitropical and tropical areas. We investigated the
genetic basis for abdominal white scale presence that represents the diagnosti...
The first confirmed dengue outbreak in nearly 70 years in Japan was reported in 2014 with 162 autochthonous dengue cases. The largest number of confirmed cases were reported in August and continued to be reported through October. Majority were associated with Yoyogi Park, a forested park popular with international and local visitors and an ideal si...
Annual changes in the distribution and abundance of mosquitoes were studied in rice field areas of southern Miyagi and Minamisouma in Fukushima, Japan, where urban and rural environments were destroyed by the Tsunami caused by the Great East Japan Earthquake in 2011. A total of 16 species and species groups of mosquitoes were collected by dry-ice t...
A nationwide survey of mosquito distribution in Japan was carried out in 2013 and 2014, in order to determine the current distribution of vector mosquitoes. Forty-two study sites located either in coastal or inland areas were selected from Hokkaido to Kyushu, and mosquitos were collected using CDC-like traps with 1 kg of dry ice (dry ice traps), a...
The aftereffects of the Tsunami on the chironomid fauna were studied in the suburban Sendai area in Miyagi Prefecture and Minami-Souma area in Fukushima Prefecture, Japan during June 4–6 in 2013. A total of 31 individuals of 7 species were collected at 13 sampling stations. At the suburban Sendai area, we could mainly collect the larvae of Chironom...
Background:
Malaria infection in mosquitoes is traditionally detected by microscopic examination for Plasmodium oocysts and sporozoites. Although PCR is now widely used, the presence of parasite DNA in a mosquito does not prove that sporogony is achieved. Thus, detection of sporozoites by microscopy is still required to definitively identify vecto...
Global warming threatens to increase the spread and prevalence of mosquito-transmitted diseases. Certain pathogens may be carried by migratory birds and transmitted to local mosquito populations. Mosquitoes were collected in the northern Philippines during bird migration seasons to detect avian malaria parasites as well as for the identification of...
An autochthonous dengue patient was confirmed on 8 September 2014 in Chiba City, Japan and emergency vector control of dengue fever against Aedes albopictus was implemented in the residential area where the case was found. We examined the distribution of human dwellings and areas with trees and bushes in the residential area by using an aerial phot...
A total of 160 autochthonous dengue cases transmitted by Aedes albopictus were reported from August to October 2014 in Tokyo Metropolis, Japan. Ae. albopictus is a medically important vector of dengue virus which has been expanding its geographic distribution in temperate regions. The understanding of the distribution and density of biting Ae. albo...
This paper evaluated the risk or probability that Aedes albopictus infect a human with dengue virus based on the results of human bait-sweep net collection of Ae. albopictus in Yoyogi area during the dengue outbreak in September 2014. The risk of dengue infection was defined by using the formula describing the probability of collecting at least one...
Knowing the genetic variation that occurs in pathogen populations and how it is distributed across geographical areas is essential to understand parasite epidemiology, local patterns of virulence, and evolution of host-resistance. In addition, it is important to identify populations of pathogens that are evolutionarily independent and thus ‘free’ t...
A German traveler developed dengue fever in late August 2013, following a direct flight from Germany. Autochthonous dengue virus (DENV) infection has not been reported in Japan. To evaluate the risk of autochthonous DENV transmission in Japan, the authors performed a retrospective search of the five areas visited by the German patient to determine...
An orbivirus was isolated from a sample from the ornithophilic mosquito Culex sasai in Japan. The virus, designated Koyama Hill virus (KHV), replicated to high titer in a mosquito cell line and to a low titer in an avian cell line, but the release of progeny viruses was not observed in mammalian cell lines inoculated with KHV. Electron microscopic...
The occurrence of natural hybridization between the mosquitoes Culex pipiens pallens and Cx. pipiens f. molestus in Northwestern Kyushu, Japan, was confirmed based on morphology and molecular methods. In order to assess the morphological difference in male genitalia of the two forms on the basis of the dorsal/ventral (D/V) ratio, mosquito egg rafts...
Mosquitoes were collected weekly from May 2003 to December 2013 at a pair of sites within the premises of the National Institute of Infectious Diseases, Tokyo, Japan. The mosquito trap was equipped with 1 kg dry ice and battery-operated suction mechanism. A total of 10,546 adults belonging to 7 species in 3 genera were collected in this study. Aede...
The invasion of the yellow fever mosquito Aedes aegypti at Narita International Airport, Japan was detected for the first time. During the course of routine vector surveillance at Narita International Airport, 27 Ae. aegypti adults emerged from larvae and pupae collected from a single larvitrap placed near No. 88 spot at passenger terminal 2 on Aug...
Outbreaks of Culex inatomii occurred widely in disaster areas of the tsunami caused by the Great East Japan earthquake in March 2011. Mosquitoes were collected in southern Miyagi Prefecture and northern Fukushima Prefecture in August and September 2011, respectively. In southern Miyagi Prefecture, the average number of adult Cx. inatomii collected...
Japanese encephalitis virus (JEV) infection in mosquitoes was monitored in Vietnam from 2006 to 2008. A total of 15,225 mosquitoes, identified as 26 species in five genera were collected and 12,621 were grouped into 447 pools for examination of JEV infection by assays for cytopathic effects in C6/36 cells and by reverse transcription-polymerase cha...
An individual marking method by placing spots of paint at five different sites on the thorax of mosquitoes was applied and a short-term ecological study on movement of Ae. albopictus and Armigeres subalbatus was conducted during 18 to 27 March 2013 in a residential area on Ishigaki island, Japan. There were 2 preservation areas with vegetation, hum...
Culex pipiens pallens is a member of the Cx. pipiens complex in Japan. e ecological conditions favorable for overwintering of Cx. p. pallens in urban areas are unknown. In two culverts of an urban environment in Saitama Prefecture, we collected 163 and 65 overwintering Culex adult mosquitoes in February 2005, and 133 and 51 in early March 2006, res...
We investigated for the first time the prevalence of avian haemosporidia of genera Plasmodium, Haemoproteus, and Leucocytozoon among birds and mosquitoes on Tsushima Island of Japan, which is located between Japan and the Korean Peninsula. Of 55 wild birds belonging to 33 species, 16 (29.1%) tested positive for haemosporidia as follows: Plasmodium...
The ecology and geographical distribution of disease vectors are major determinants of spatial and temporal variations in the transmission dynamics of vector-borne pathogens. However, there are limited studies on the ecology of vectors that contribute to the natural transmission of most vector-borne pathogens. Avian Plasmodium parasites are multiho...
We established a continuous cell line from the embryo of the mosquito Culex tritaeniorhynchus Giles (Diptera: Culicidae), a known major vector of the Japanese encephalitis virus (family Flaviviridae, genus Flavivirus) in Asia. The cell line, designated NIID-CTR, was serially subcultured in VP-12 medium supplemented with 10 % heat-inactivated fetal...
The seasonal prevalence of Aedes albopictus (Skuse) and the temporal and spatial variation of biting density were studied in an urban park in Tokyo, Japan. Human landing collections were conducted monthly at 16 collection sites in Rinshino-mori Park from May/June to November during 2003 to 2005. Biting females appeared in May/June and the density p...
To investigate the possible spread of West Nile virus (WNV) into Japan, we carried out entomological surveillance for flaviviruses at migratory bird stopover sites in Hokkaido, Japan, during 2003-2006. A total of 3,826 mosquitoes, identified as 15 species in five genera, were collected and 2,465 of these were grouped into 123 pools that were assaye...
The Tsunami caused by the Great East Japan Earthquake on 11 March 2011 in north-eastern Japan destroyed urban and rural environments completely, including buildings, dwellings, roads, bridges, agricultural fields and natural vegetation. The after-effects of the Tsunami on the distribution and abundance of mosquitoes were studied in rice field areas...
The 2003-2004 H5N1 highly pathogenic avian influenza (HPAI) outbreaks in Japan were the first such outbreaks in 79 years in Japan. Epidemic outbreaks have been occurring in Southeast Asia, with the most recent in 2010. Knowledge of the transmission route responsible for the HPAI outbreaks in these countries remains elusive. Our studies strongly sug...
During a mosquito collection, a female of the pigeon louse fly, Pseudolynchia canariensis (Diptera: Hippoboscidae), was collected by a mosquito trap baited with dry ice in Ishigaki-jima, Yaeyama Islands, Japan. This is the 1st record of P. canariensis from Yaeyama Islands.
We studied the prevalence of avian Plasmodium in 509 mosquitoes of 9 species collected from the Ishigaki and Iriomote islands in the Yaeyama Archipelago, located southwest from the mainland of Japan. Two identical avian Plasmodium lineages were detected from Culex (Culiciomyia) nigropunctatus. Detected lineages were phylogenetically classified into...
Among members of the order Mononegavirales, RNA splicing events have been found only in the family Bornaviridae. Here, we report that a new rhabdovirus isolated from the mosquito Culex tritaeniorhynchus replicates in the nuclei of infected cells and requires RNA splicing for viral mRNA maturation. The virus, designated Culex tritaeniorhynchus rhabd...
In Japan, the prevalence of avian Plasmodium in birds and mosquitoes has been partially examined in the temperate and subtropical zones; however, mosquitoes in the Japanese subarctic zone have not been adequately investigated. In this study, mosquito collections and avian Plasmodium detections from the mosquito samples were carried out to demonstra...
Several species of captive and wild birds have been found to be infected with various avian blood protozoa in Japan. We investigated the prevalence and transmission of avian malaria parasite and determined the bloodmeal hosts of mosquitoes collected in a zoological garden in Tokyo, Japan, by using the polymerase chain reaction. In total, 310 unfed...
Heterogeneity in the transmission of mosquito-borne pathogens is determined largely by distribution patterns of mosquito bites among wild animal populations. Although mosquitoes are crucial for transmission of avian malaria parasites, little is known about the ecology of natural vectors. We examined bloodmeal and parasite incidence in Culex pipiens...
A laboratory colony of the mosquito Aedes japonicus japonicus, which has recently invaded the United States and is recognized as a highly competent vector of West Nile virus, was established from larvae collected in Narita, Japan. The mosquitoes were maintained with induced insemination, blood-feeding on humans, and oviposition in water provided fr...
Species E of the Anopheles minimus sibling species complex is diagnosed and formally named An. yaeyamaensis Somboon & Harbach, sp. n. Information is provided on the morphology, genetics and bionomics of the species, which has a restricted distribution in the Yaeyama and Miyako Islands located at the southern end of the Ryukyu Archipelago of Japan.
Internal transcribed spacer regions of ribosomal DNA were sequenced, and new species-specific primers were designed to simplify the molecular identification of five morphologically related subgenus Stegomyia mosquito species--Aedes aegypti, Ae. albopictus, Ae. riversi, Ae. flavopictus, and Ae. daitensis--found in the Ryukyu Archipelago, Japan. Each...
Two infectious agents were isolated from Culex species mosquitoes in Japan and were identified as distinct strains of a new RNA virus by a method for sequence-independent amplification of viral nucleic acids. The virus designated Omono River virus (OMRV) replicated in mosquito cells in which it produced a severe cytopathic effect. Icosahedral virus...
A severe dengue outbreak occurred in East Timor in 2005. The dengue virus genome was detected by TaqMan RT-PCR in 40 serum samples, as follows: dengue virus type-3 (DENV-3) in 37 samples, DENV-2 in 2 samples, and DENV-1 in one sample. One DENV-1 genome, one DENV-2 genome, and 5 DENV-3 genomes were sequenced, and these specimens were aligned with th...
To evaluate the vectorial capacity of mosquitoes for viruses in Japan, the host-feeding habits of the mosquitoes were analyzed by sequencing polymerase chain reaction-amplified fragments of the cytochrome b and 16S ribosomal RNA regions of the mitochondrial DNA of 516 mosquitoes of 15 species from seven genera that were collected from residential a...