Yoiner K. Lapiz-Culqui’s research while affiliated with Universidad Nacional Toribio Rordríguez De Mendoza and other places


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Publications (3)


Wild blackberry fruits of (a) R. andicola, (b) R. floribundum, (c) R. andenothallus, and (d) R. webebaueri. The arrow indicates the maturity stage of fruits collected for analysis.
Diameter of antibacterial inhibition of wild blackberry extract against (a) Staphylococcus aureus and (b) Escherichia coli bacteria. The discs are labeled 1 for R. webebaueri, 2 for R. andicula, 3 for R. andenothallus, 4 for R. floribundum, and 5 for distilled water. The center disk includes the antibiotic ampicillin and oxacillin.
Effect of different concentrations of 6-benzylaminopurine on in vitro multiplication of four wild blackberry species. Panels are (a) number of shoots, (b) shoot height, (c) number of leaves, (d) loss rate and (e) in vitro plant regeneration. (e1) R. andicola, (e2) R. floribundus, (e3) R. adenothallus, and (e4) R. weberbaueri.
Study of the physicochemical characteristics, antimicrobial activity, and in vitro multiplication of wild blackberry species from the Peruvian highlands
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February 2024

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98 Reads

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1 Citation

Scientific Reports
Yoiner K. Lapiz-Culqui

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The Peruvian Andes are the natural habitat of several wild blackberry species that are little known and exploited due to the lack of technological and scientific development to support their agricultural potential. In this context, a study was conducted to understand the physicochemical composition, bioactive compounds, antimicrobial activity, and in vitro multiplication of four wild blackberry (Rubus sp.) species from the northern Peruvian highlands. The results indicate that fruits of R. floribundus presented the highest content of total soluble solids (9.58 ± 1.83°Brix) and titratable acidity (1.88 ± 0.07% citric acid). The fruits of R. weberbaueri recorded the highest total phenolic content (415.06 ± 8.69 mg GAE/100 g Ff). The antioxidant capacity determined by the DPPH assay varied significantly among species, with the highest value found in fruits of R. andicola (50.27 ± 0.11 mg TE/100 g Ff). The fruit extracts of R. weberbaueri and R. andicola showed better antimicrobial activity, with Staphylococcus aureus being the most sensitive bacterium. In the in vitro multiplication phase, the results show that BAP (6-Benzylaminopurine) has a significant effect at a dose of 1.5 mg l⁻¹ on shoot number, leaf number, and shoot length. The results may help in the management of genetic resources.

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In Vitro Bulbification of Five Lily Varieties: An Effective Method to Produce Quality Seeds and Flowers

September 2022

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154 Reads

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7 Citations

Lilies are one of the most important, beautiful, and economically valuable flowers in the world. Lilium is regarded as a popular floral trade cut flower, so viable protocols are needed to provide seed production, multiplication, and preservation. In vitro protocols allow for rapid large-scale production and rejuvenation of planting material, but to be a commercially viable multiplication method, the procedure must allow for rapid production of viable, true-to-type plants quickly. The objective was to evaluate the in vitro production of microbulbs of five lily varieties (Lilium “Champion Diamond,” Lilium “Yellow Diamond,” Lilium “Batavus,” Lilium “Hyde Park,” and Lilium sp.) using different concentrations of 6-benzylaminopurine (0, 0.5, 1.0, 1.5, 1.5, and 2.0 mg L−1 BAP) and to determine the commercial quality (flowering) of the plants grown from the seed obtained. Results from the micropropagation phase show Lilium “Batavus” and Lilium “Hyde Park” varieties had better in vitro responses, especially when grown with 1.0 and 1.5 mg L−1 BAP, respectively. Plants (of all varieties) grown from microbulbs showed positive growth and generally resulted in commercially viable flower production. Finally, the results of this study support the use of bulb scales as an alternative for obtaining vegetative seeds with high potential for lily cultivation.


Fig. 1. In vitro propagation of Vaccinium corymbosum L. (a) Parent plant, (b) Explants, (c) Establishment, (d) In vitro multiplication, (e) In vitro rooting, (f) Ex vitro acclimatization, (g) Regenerated plant in nursery stage. To the left = 'Bluecrop', To the Right = 'Biloxi'.
Fig. 3. Blueberry plant height on different substrates during ex vitro acclimatization. (a) 'Bluecrop' and (b) 'Biloxi'.
Fig. 4. Influence of substrates on morphological parameters of blueberry seedlings (varieties 'Biloxi' and 'Bluecrop') during ex vitro acclimatization. (a) Number of shoots, (b) Leaf area, (c) Number of roots, (d) Root length, (e) Fresh weight of the aerial part, (f) Dry weight of the aerial part, (g) Fresh weight of the root system, and (h) Dry weight of the root system. Substrate 1: saw dusk + peat; Substrate 2: burn rice husk + peat; Substrate 3: raw rice husk + peat; Substrate 4 = cocomix® + peat; Substrate 5: perlite + peat.
Fig. 5. Influence of substrates on physiological parameters of blueberry seedlings (varieties 'Biloxi' and 'Bluecrop') during ex vitro acclimatization. (a) Chlorophyll a, (b) Chlorophyll b, (c) Chlorophyll a+b, (d) Carotenoids, (e) SPAD index, (f) Stomatal density, (g) Stomatal index, (h) Stomatal conductance, and (i) Relative water content. Substrate 1: saw dusk + peat; Substrate 2: burn rice husk + peat; Substrate 3: raw rice husk + peat; Substrate 4 = cocomix® + peat; Substrate 5: perlite + peat.
Fig. 6. Correlation analysis among studied the morpho-physiological parameters in blueberry seedlings variety (a) 'Bluecrop' and (b) 'Biloxi' during ex vitro acclimatization. NS: Number of shoots; PH: Plant height; LA: Leaf area; NR: Number of roots; RL: Root length; FWAP: Fresh weight of the aerial part; DWAP: Dry weight of the aerial part; FWRS: Fresh weight of the root system; DWRS: Dry weight of the root system; SI: Stomatic index; SD: Stomatic density; RWC: Relative water content; SC: Stomatic conductance; SPAD: Chlorophyll index; Chl-a: Chlorophyll A; Chl-b: Chlorophyll B; Chl-a+b: Total chlorophyll; Cart.: Carotenoids.
Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

June 2022

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263 Reads

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1 Citation

Journal of Berry Research

BACKGROUND: Blueberry production has generated great commercial expectations, therefore for its agricultural expansion it is necessary to overcome the challenges at the time of mass propagation. OBJECTIVE: Evaluate the effect of a set of factors influencing micropropagation, as well as the influence of substrates on the ex vitro morpho-physiological performance of blueberry seedlings. METHODS: A set of protocols were developed to optimize all stages of micropropagation (aseptic establishment, multiplication, rooting, and acclimatization) of blueberries. RESULTS: Explants immersed in 1.5% NaClO for 8 min and then in 0.1% HgCl2 for 2 min achieved 100% sterility and a viability rate of 86.67% for ‘Biloxi’ and 93.33% for ‘Bluecrop’. At the multiplication stage, the maximum number of shoots of ‘Biloxi’ (3.53) and ‘Bluecrop’ (2.27) were obtained on the medium supplemented with 0.2 and 10 mg L–1 silver nanoparticles (AgNPs), respectively. The percentage of in vitro rooting was significantly improved on media containing activated charcoal, with levels between 80% and 100% . In the acclimatization phase, plants grown in a substrate composed of peat and cocomix® (2:1 ratio) showed greater uniformity and better morpho-physiological behavior. CONCLUSIONS: The present results could be successfully used for large-scale commercial production of blueberries of the varieties ‘Biloxi’ and ‘Bluecrop’.

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Citations (3)


... Regarding tannins, their presence is an added value since they have the ability to avoid the growth of several bacteria, fungi, yeasts, and viruses (Gil-Martínez et al., 2023;Kaczmarek, 2020;Lapiz-Culqui et al., 2024). ...

Reference:

Assessment of chemical composition of blackberries and mulberries from Covilhã region, Portugal
Study of the physicochemical characteristics, antimicrobial activity, and in vitro multiplication of wild blackberry species from the Peruvian highlands

... Lilium "Yellow Diamond," Lilium "Batavus," Lilium "Hyde Park," ve Lilium sp.) in mg.l⁻¹) içeren çeşitli büyüme düzenleyici maddeler kullanmıştır. Çalışmanın sonucunda, en başarılı ortamın 1-1,5 mg/litre BAP içeren ortam olduğu sonucuna ulaşılmıştır[19]. Bu çalışma, farklı zambak çeşitlerinin in vitro soğan oluşumu için etkili bir yöntem geliştirme amacını taşımaktadır. ...

In Vitro Bulbification of Five Lily Varieties: An Effective Method to Produce Quality Seeds and Flowers

... Одновременно с этим, увеличиваются и исследования по развитию плодово-ягодных растений на разных этапах этой технологии. До сих пор мало исследований по доращиванию ex vitro растений рода Vaccinium L. в условиях защищенного грунта [57,58,59,60], поэтому мы провели исследования в данной области. ...

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

Journal of Berry Research