Yew‐Seng Lai's research while affiliated with National Taiwan University and other places

Publication (1)

Article
Silicon nanowire field-effect transistors modified with specific aptamers can directly detect the minute dopamine and neuropeptide Y released from cells. The binding of these molecules to the aptamers results in a conductance change of the transistor biosensor and illustrates the differential releasing mechanisms of these molecules stored in variou...

Citations

... The linker chemistry for binding aptamer probes on the FET surface depends on the available functional groups on the aptamers. In the case of thiolated DNA aptamers [49,91,138], a two-molecule linker is usually used (Fig. 6a): first, an amine-terminated silane monolayer (e.g., APTMS) is formed on the gate oxide; then, MBS is reacted with the amine group to impart maleimide groups to the sensor surface. These maleimide groups, in turn, crosslink with the thiol group of the aptamers, thereby immobilizing the aptamers to the sensor surface. ...