Yao Fu's research while affiliated with Renji Hospital and other places

Publications (61)

Article
Background/Aims This study aimed to describe a cohort of patients with cryptophthalmos, characterize associated oculofacial abnormalities, and expand the classification to summarize surgical strategies for managing cryptophthalmos. Methods A retrospective, interventional case series was conducted on 86 patients (124 eyes) with cryptophthalmos. The...
Article
Persistent corneal epithelial defects (PED) can lead to irreversible blindness, seriously affecting the social function and life quality of these patients. When it comes to refractory PED, such as limbal stem cell deficiency (LSCD), that does not respond to standard managements, stem cell therapy is an ideal method. Oral mucosal epithelium (OME) ab...
Article
Topical drug delivery is critical for treating ocular diseases but suffers from significant defects including low drug availability, long-term frequent administration, and damage to ocular surface, which largely restrict treatment efficacy and influence quality of life. Here, we describe the formation of a durable and protective therapeutic nanocoa...
Article
Full-text available
Purpose: This study aimed to investigate the effect of transcutaneous upper eyelid blepharoplasty on lipid layer thickness (LLT) and blink parameters in young women during the early postoperative period. Methods: This prospective study included 110 eyes of 55 young female patients (age range, 19–31 years) who underwent transcutaneous upper eyelid b...
Article
Full-text available
Eyelid plays a vital role in protecting the eye from injury or infection. Inflammation related eyelid diseases, such as blepharitis, are the most common ocular disorders that affect human's vision and quality of life. Due to the physiological barriers and anatomical structures of the eye, the bioavailability of topical administrated therapeutics is...
Article
Full-text available
Extracellular matrix (ECM) hydrogels provide advantages such as injectability, the ability to fill an irregularly shaped space, and the adequate bioactivity of native matrix. In this study, we developed decellularized cartilage ECM (dcECM) hydrogels from porcine ears innovatively via the main method of enzymatic digestion and verified good biocompa...
Article
The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes. In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP). The CNF coating improved the hydrophi...
Article
Full-text available
Purpose: This study aimed to figure out the association between ophthalmic and pathological features in patients with Linear Nevus Sebaceous Syndrome (LNSS) and in patients with Oculoectodermal Syndrome—Encephalocraniocutaneous Lipomatosis (OES-ECCL). Methods: It is a retrospective, non-consecutive, observational case series. Twenty-seven patients...
Article
Full-text available
Purpose: The present study was designed to observe the curative effect of early intralesional corticosteroid injection to treat unsatisfactory double eyelid. Methods: This retrospective, observational study included 30 female patients (age 22–35 years) receiving intralesional corticosteroid injection after complaining about the unsatisfactory doubl...
Article
Full-text available
Background Sufficient vascular network plays an important role in the repair of bone defects. Bone morphogenetic protein 2 (BMP2) being a key regulator of angiogenesis has attracted the attention of researchers. In addition, evidence has suggested that BMP2 coordinates with microRNAs (miRNAs) to form intracellular networks regulating mesenchymal st...
Article
Full-text available
Purpose: This study aimed to investigate the therapeutic effects and underlying mechanisms of locally delivered regulatory T cells (Tregs) on acute corneal wound healing after alkali burn. Methods: After corneal alkali burn, the mice were injected subconjunctivally with regulatory T cells (Tregs) isolated from syngeneic mice. The wound healing p...
Article
Full-text available
Background Fat grafting, as a standard treatment for numerous soft tissue defects, remains unpredictable and technique-dependent. Human adipose-derived stem cells (hADSCs) are promising candidates for cell-assisted therapy to improve graft survival. As free-living fat requires nutritional and respiratory sources to thrive, insufficient and unstable...
Article
Full-text available
Purpose: The aim of this study was to compare the ocular microbial communities in humans with and without demodex blepharitis in order to elucidate the relationship between ocular microorganisms and demodex infestation. Methods: Bacterial 16S rRNA genes of conjunctival sac samples from 30 demodex blepharitis patients and 14 healthy controls were se...
Article
Purpose: To investigate the proliferation of umbilical cord blood-derived endothelial progenitor cells (UCB EPCs) and the differentiation efficiency toward corneal endothelial cell (CEC)-like cells induced by rho-associated protein kinase (ROCK) inhibitor Y-27632 and to determine the most effective strategy for repairing corneal endothelium injuri...
Article
Full-text available
Treating corneal endothelial diseases tends to be challenging as human corneal endothelial cells (CECs) do not proliferate in vivo. The pathogenesis or mechanisms underlying injured CECs need further studies. The abnormal expression of PAX6, which is an essential transcription factor for corneal homeostasis, exhibits corneal endothelial defects. Ho...
Article
Conjunctival restoration is indispensable to help maintain the ocular surface microenvironment by secreting lubricative mucins. However, conventional amniotic membrane transplantation therapy has many limitations in its application due to risks involved with disease transmission and alloreactivity. As decellularized tissues have been frequently use...
Article
Purpose We reported the occurrence of a congenital unilateral huge peripapillary staphyloma in association with craniofacial clefts for the first time. Case report A 1-year-old boy presented with a large defect on his left eyelid, a wide oblique columella nasi and an atypical wedge-shaped extension of the unilateral anterior hairline. Magnetic res...
Article
Background Dry eye symptoms as a complication of double-eyelid blepharoplasty are controversial. This article evaluates tear film dynamics and changes in dry eye symptoms after cosmetic double-eyelid blepharoplasty in young Asian female.Methods One hundred and twenty patients underwent full-incisional double-eyelid blepharoplasty. Subjective and ob...
Article
Full-text available
Purpose: To investigate the role of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) in the regulation of corneal endothelial cell (CECs) focusing on proliferation and migration, and to further evaluate the application of PTEN inhibitors in the treatment of corneal endothelial dysfunction in a rat model. Methods: Expression of PT...
Article
Full-text available
Conjunctival fibrosis is a process of extracellular matrix accumulation and the appearance of myofibroblasts in subconjunctival fibroblasts induced by injury or inflammation, which can significantly reduce the filtration efficiency of glaucoma filtration surgery. In this study, autophagy was confirmed to be involved in regulating the fibrosis of hu...
Article
Full-text available
Severe conjunctival diseases can cause significant conjunctival scarring, which seriously limits eye movement and affects patients' vision. Conjunctival reconstruction remains challenging due to the lack of efficient methods for stem cells enrichment. This study indicated that p75 positive conjunctival epithelial cells (CjECs) were mainly located i...
Article
Full-text available
Purpose: To evaluate episcleral vasculature in corneal limbus with optical coherence tomography angiography (OCTA) in normal controls, port-wine stain (PWS) patients, and Sturge-Weber syndrome (SWS) patients. Methods: Unilateral eyes from 18 normal controls (25.41 ± 4.00 years), 16 PWS patients (21.35 ± 11.05 years), and 8 SWS patients with ipsi...
Article
Full-text available
Tarsal plate regeneration has always been a challenge in the treatment of eyelid defects. The commonly used clinical treatments such as hard palate mucosa grafts cannot achieve satisfactory repair effects. Tissue engineering has been considered as a promising technology. However, tarsal plate tissue engineering is difficult to achieve due to its co...
Article
Full-text available
Purpose: To evaluate changes in corneal sensitivity and subbasal nerve density after pterygium excision. Methods: This prospective trial included 22 eyes with nasal primary pterygium and 18 controls. Corneal sensitivity was evaluated using a Cochet-Bonnet esthesiometer in the nasal, superior, temporal, inferior, and center quadrants of the corne...
Article
Corneal and conjunctival infections are common ocular diseases, sometimes, causing severe and refractory drug-resistant bacteria infections. Fungal keratitis is a leading cause for blindness and traditional medical treatment is unsatisfactory. Thus, there is an urge to develop a new therapy to deal with these cases. In this study, we developed surf...
Article
Purpose: Facial paralysis (FP) leads to diverse periocular complications which threats visual acuity and affects corneal nerve functionally and morphologically. This study aims to summarize the clinical ophthalmic outcomes, corneal sensation, and morphological alterations of subbasal nerve and dendritic cells (DCs) in patients with facial paralysis...
Article
Full-text available
The determination of potential transplantable substrates and substitution cells for corneal endothelium transplantation may compensate for the shortage of cornea donors. Appropriate biodegradable and biocompatible tissue-engineered substratum with seed cells for endothelial keratoplasty has been increasingly studied. In the present study, electrosp...
Article
Neovascularization during ocular tissue repair can cause severe visual loss in the optical axis and is therefore an issue of considerable concern to ophthalmologists. Here, we introduced a cholesterol-modified siRNA delivery system targeting stromal cell-derived factor 1 (SDF-1) to treat ocular angiogenesis in vivo. SDF-1 expression was analyzed in...
Article
Full-text available
Purpose To investigate the impact of disease duration on the ocular surface during the course of type 2 diabetes mellitus compared with nondiabetic controls. Methods One hundred twenty diabetic patients were divided into three groups according to disease duration: less than 5 years, 5–10 years, and over 10 years. All eyes were imaged using a corne...
Article
Full-text available
Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in tissue engineering. In this study, silk fibroin (SF) and poly(L-lactic acid-co-ε-caprolactone) (PLCL) hybrid scaffolds were successfully prepared by electrospinning. Scanning electron micrographs (SEM) showed that SF/PLCL sca...
Article
Full-text available
Conjunctival injuries are general but intractable ocular surface diseases, the sequelae of which are particularly challenging to treat. A promising therapy for conjunctival injuries is to employ biodegradable scaffolds to deliver conjunctival epithelial cells for repairing damaged or diseased conjunctiva. In the present study, an ultrathin porous n...
Article
Purpose: To develop a new rabbit model of corneal endothelial injury using a neodymium-doped yttrium aluminum garnet (Nd:YAG) laser. Methods: The corneal endothelia of 2 groups of New Zealand white rabbits were treated with an Nd:YAG laser in a uniformly scattered fashion. Rabbits in group A underwent laser burns on the whole corneal endothelium...
Article
Full-text available
The present study aimed to investigate the efficacy of tailored treatment for the management of scleral necrosis following pterygium surgery. A series of nine cases of scleral necrosis following pterygium excision between September 2009 and September 2012 were included. In cases where ischemia was the cause of scleral necrosis, Tenon’s membrane cov...
Article
Aim: The aim of this study was to evaluate the long-term outcome when a free tarsomarginal graft is used to repair a large congenital coloboma in patients with a Tessier number 10 cleft. Materials and methods: This was a retrospective, interventional case series. The medical records were reviewed for five children (six eyes) diagnosed as having...
Article
Full-text available
Aim: To explore the effects of conditioned media on the proliferation of corneal endothelial cells (CECs) and to compare the efficiency of different conditioned media (CM). Methods: Rat CECs, corneal stromal cells (CSCs), bone marrow-derived endothelial progenitor cells (BEPCs), and bone marrow-derived mesenchymal stem cells (BMSCs) were isolate...
Article
Full-text available
Biological scaffolds composed of extracellular matrix (ECM) are often used to facilitate wound healing in a variety of tissues in clinical and preclinical studies. Recently, ECM hydrogels have been widely used because of their advantages, such as a variable composition, good biocompatibility, and the ability to fill an irregularly shaped space. The...
Article
Full-text available
Purpose: To study the feasibility of engineering conjunctival epithelial cell sheets on a temperature-responsive culture dish for ocular surface reconstruction. Methods: Rabbit conjunctival epithelial cells (rCjECs) were cultured in DMEM/F-12 (1:1) medium. The morphology and phenotype of the rCjECs were confirmed with phalloidin staining, period...
Article
Corneal transparency is maintained by the corneal endothelium through its barrier and ionic pump function. However, this function could be compromised with age and variety of diseases and trauma, leading to cornea dycompensation, corneal edema, bullous keratopathy and even loss of visual acuity. So far, a lot of measures have been proposed to solve...
Article
Full-text available
Cornea transplant technology has progressed markedly in recent decades, allowing surgeons to replace diseased corneal endothelium by a thin lamellar structure. A thin, transparent, biocompatible, tissue-engineered substratum with corneal endothelial cells for endothelial keratoplasty is currently of interest. Electrospinning a nanofibrous structure...
Article
Full-text available
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have extensive self-renewal capacity and the potential to differentiate into all tissue-specific cell lineages, including corneal endothelial cells (CECs). They are a promising prospect for the future of regenerative medicine. The method of derivation of CECs from ESCs and iPSCs...
Article
Corneal endothelial dysfunction involves progressive corneal edema and loss of visual acuity, which result in the need for corneal transplantation. The global shortage of donor corneas limits the development of the surgery. Reconstruction of a bioengineered corneal endothelium might resolve this problem. Various scaffolds have been used, but poor b...
Article
Full-text available
Purpose: To investigate the effect of the ATP-P2Y2-PI3K/Akt signaling axis on promoting rabbit corneal endothelial cell (RCEC) proliferation in vitro. Methods: Five concentrations of adenosine triphosphate (ATP; 1, 10, 25, 50 and 100 μM) were added to RCECs, and the cell proliferation was detected using Cell Counting Kit-8 (CCK8) and Ki67 immuno...
Article
To investigate the dynamic changes of tear fluid after cosmetic transcutaneous lower blepharoplasty. Prospective, Observational Case Series study. A total of 60 eyes of 30 patients with transcutaneous lower blepharoplasty were involved in the study. All subjects completed the Ocular Surface Disease Index questionnaire and underwent tear film breaku...
Article
Blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) is a rare autosomal dominant condition characterized by typical eyelid malformations that include blepharophimosis, ptosis, epicanthus inversus, and telecanthus. We retrospectively reviewed 125 consecutive BPES patients who underwent staged surgical intervention from July 2003 to December...
Article
Bone graft substitutes with osteogenic factors alone often exhibit poor bone regeneration due to inadequate vascularization. Combined delivery of osteogenic and angiogenic factors from biodegradable scaffolds may enhance bone regeneration. We evaluated the effects of bone morphogenetic protein 2 (BMP2) and vascular endothelial growth factor (VEGF),...
Article
The global shortage of donor corneas has motivated the development of bioengineered corneas. Although corneal endothelium has been reconstituted using corneal endothelial cells (CEC) and precursor cells with various carrier materials, all of the current options require corneal tissue and are also limited by the scarcity of donor corneas. Here, we e...
Article
Interest in developing tissue-engineered cornea has increased with the decrease in the supply of donor tissue. The aim of the present study was to investigate the feasibility and method of reconstructing corneal equivalents with porcine corneal acellular matrix as the scaffold in a dynamic culturing system. Applying the detergent Triton X-100 (1%)...
Article
To test the effects of VEGF-siRNA-transfected corneal epithelium on corneal neovascularization (CNV). It was an experimental study. Cultured rat corneal epithelial cells and keratocytes were transfected with synthesize VEGF siRNA by lipofectamine 2000. The level of VEGF mRNA was analyzed by real time PCR, and the protein levels were determined by e...
Article
To study the clinical manifestations and surgical treatment of Type II orbital neurofibromatosis. From Jan, 2001, to Oct, 2006, clinical data of 16 cases with type II orbital neurofibromatosis were retrospectively analyzed in the Department of Ophthalmology, Ninth People's Hospital, Medical School of Shanghai Jiao Tong University. All patients had...
Article
To evaluate the security and efficacy of surgical procedure combined with Nd:YAG laser in the management of orbital venous malformation. A total of 17 successive patients with orbital venous malformation were treated with surgical excision in conjunction with Nd:YAG laser from July 2003 to June 2005. General anesthesia was performed in all patients...
Article
To present the clinical manifestations and outcome of the surgical management of subjects with Tessier number 10 clefts. Retrospective, interventional case series. Twelve patients with Tessier number 10 clefts treated at the Department of Ophthalmology of Shanghai Ninth People's Hospital between January of 2002 and December of 2005. All 12 patients...
Article
To investigate the safety and efficacy of surgical management of orbital neurofibroma (NF). Twenty-six patients with orbitotemporal NF underwent tumor resection. And then 12 of the 26 patients that were of Jackson group I, underwent blepharoplasty surgery, including lid plastic and reconstructive surgery, lateral canthal reattachment and ptosis sur...
Article
To analyze the incidence and pattern of lacrimal pathways obstruction after naso-orbital-ethmoid fractures (NOE), and to investigate the relationship between lacrimal obstruction and fracture types. One hundred and one patients with NOE fractures were analyzed retrospectively. These patients were treated in the Department of Ophthalmology, Ninth Pe...
Article
To analyze the outcomes of lacrimal bypass surgery using the high-density porous polyethylene (HDPP) coated tear drain and to evaluate the validity and safety of this procedure. Twenty-six patients (26 eyes) with severe epiphora who could not be cured by canaliculo-anastomosis and dacryocystorhinostomy were treated by the lacrimal bypass surgery wi...
Article
To investigate the method and feasibility of constructing biological cornea by culturing corneal epithelial and endothelial cells on the scaffold of xenogenic corneal acellular matrix (XCAM) in vitro. Porcine cornea was prepared as XCAM by application of detergent 1% Triton X-100 and freeze-drying process. After the carrier has rehydrated, rabbit e...
Article
To construct three-dimensional (3D) imaging and computer generated models of complex orbital fractures, and develop a Computer-Aided Design/Computer-Aided Manufacture (CAD/CAM) system to help improve the surgical planning of complex orbital fracture and promote its outcome. A prospective study was carried out on 17 patients with unilateral complex...
Article
To investigate the feasibility of using amniotic membrane (AM) as a carrier for cultured corneal endothelial cells transplantation. Rabbit corneal endothelial cells were cultivated, passaged, and labeled with the fluorescent tracker CM-DiI, then transplanted onto denuded AM. The cell density, morphology, histology and ultrastructure of the cultured...
Article
To evaluate the surgery methods and effectiveness of medial canthal deformities after naso-orbital-ethmoid fractures (NOE) and analysis the long-term results. The clinical data and following-up results from 83 patients (83 eyes), which were treated in the Department of Ophthalmology, Ninth People's Hospital, Medical School of Shanghai Jiao Tong Uni...

Citations

... Previous studies also showed the application of NPs in ocular diseases, such as cerium oxide NPs for aged-related macular degeneration (AMD), gold NPs for uveitis and AMD, and sliver NPs for fungal keratitis (Pereira, Petronilho et al., 2012;Tisi, Passacantando et al., 2019;Singh R et al., 2020;Shi, Ding et al., 2021). Given NPs possess anti-oxidative stress, anti-inflammatory, anti-angiogenic, and anti-bacterial effects, and their strong permeability and biocompatibility, NPs have been applied in ocular disease treatment (Chan C. M. et al., 2019;Tisi, Passacantando et al., 2019;Zheng, Fang et al., 2019;Gharpure, Akash et al., 2020). ...
... For glaucoma studies, rabbits with both normal as well as elevated IOP were used. For DED, rabbits used were pretreated topically with eye drops containing either 0.1% benzalkonium chloride (Huang et al., 2018;Chen et al., 2021) or 1% atropine sulfate in order to induce disease symptoms (Shafaa et al., 2011;Karn et al., 2014;Kang et al., 2016). Ocular inflammation in rabbits was induced with topical application of arachidonic acid (Bucolo et al., 2002;Katara and Majumdar, 2013;Sharma A. K. et al., 2016a;Sharma AK. et al., 2016b;Weng et al., 2018;Katara et al., 2019) or endotoxin (Adibkia et al., 2007a;Adibkia et al., 2007b). ...
... The CD34 immunohistochemical staining of the rat wound sections suggests that compared to the blank group, the PADM and AgNP-PADM hydrogels significantly increased the intraconal capillary production and accelerated granulation tissue growth, thereby promoting wound healing. Various studies have shown that although homologous-tissue-derived ECM is more conducive to cell growth and promotes the production of the original extracellular matrix components [51][52][53], the specific components and mechanisms of this phenomenon are not yet fully understood. ...
... Bacterial cellulose (BC)-CS NPs composite hydrogels fabricated by γ-irradiation (20-60 kGy) showed superb antibiofilm properties, reflected in up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height and was characterized by a higher porosity, a higher wound fluid absorption and faster in vitro healing compared to respective composite hydrogels prepared with CS polymer [239]. LEV-loaded composite scaffolds of PLA electrospun nanofibrous membranes surface-coated by CNFs and/or silk peptide, in which the CNF coating ameliorated the hydrophilicity and silk peptide coating proliferated conjunctival epithelial cells exhibiting effective bactericidal effects; they were able to stimulate structural and functional restoration of conjunctiva after transplant and thus minimize the post-surgery application of antibiotics [240]. Ampicillin-encapsulated BC/PVA hydrogels ensured 30% of the cumulative antibiotic delivery during 120 h, exhibited superior antibacterial activity against S. aureus and E. coli and can be used as wound dressings with sustained antibiotic delivery [241]. ...
... With the development of endoscopy, now the intra-articular injection is convenient [54]. For therapy of inflammation related eyelid diseases, the Rosiglitazone-loaded TG-18 hydrogel can be used for in situ eyelid injection with sustained therapeutics release [55]. In addition, based on the characteristics of the hydrogel depot, the composite can also be applied on the skin or subcutaneous injection like other hydrogel [56]. ...