Yanling Huang's research while affiliated with Wuhan Institute Of Virology and other places

Publications (5)

Article
Full-text available
The novel phage lysin PlySs2, is reported to be highly active against various bacteria, including staphylococci, streptococci and Listeria. However, the molecular mechanisms underlying its broad lytic spectrum remain to be established. In the present study, the lytic activity of the catalytic domain (CD, PlySc) and binding specificity of the cell w...
Article
Full-text available
Developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing the catalytic domain (Ply187N) of the bacteriophage lysin Ply187 with the cell binding domain (146-314aa, V12C) of the lysi...
Article
Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for a large number of chronic infections due to its ability to form robust biofilms. Herein, the authors evaluated the anti-biofilm activity of a Staphylococcus specific chimeric lysin ClyH on MRSA biofilms. ClyH is known to be active against planktonic MRSA cells in vitro and in viv...
Article
Full-text available
The treatment of infections caused by methicillin-resistant Staphylococcus aureus (MRSA) is a challenge worldwide. In our search for novel antimicrobial agents against MRSA, we constructed a chimeric lysin (named as ClyH) by fusing the catalytic domain of Ply187 (Pc) with the non-SH3b-like cell wall binding domain of phiNM3 lysin. Herein, the antim...

Citations

... The use of confocal microscopy and specific staining on biofilms would allow the exploration of the composition of the biofilm and thus to match the composition with the heterogeneous responses obtained in our study. Another hypothesis is related to the mechanism of action of exebacase, which cleaves the interpeptide bridge of peptidoglycan (15,20,(36)(37)(38). Compared to S. aureus, the interpeptide bridge structure in S. epidermidis is variable (39,40), thus changes in the amino acid composition could lead to a change of activity of exebacase, as observed with lysostaphin (41), another cell wall hydrolase, or to a decreased exebacase affinity, as exebacase recognizes and binds to a specific interpeptide bridge pattern (42). ...
... Phage endolysin has more extensive host explicitness contrasted with lytic bacteriophages . Chimeric endolysins have as of late been created by melding enzymatic spaces to elective cell-dividers restricting determinants, therefore changing endolysin conduct and host go (51). These are used as natural preservatives, singly or in combination with other antimicrobials such as bacteriocin (52) CONCLUSION Thus from this study, it was observed as phage therapy of Bacteriophages had potentially used many antibiotic-resistant bacteria but the limitation if solved would prove it to be better treatment, and researchers are made through for unraveling the hidden importance and effectiveness of Bacteriophages in Human Pathogens. ...
... As seen in Table 2, S. aureus, especially multi-drug resistant S. aureus (MRSA) is one of the major target bacteria when it comes to research on lysins, as they are common in clinical settings. In studies from both 2014 and 2017, ClyH and ClyF (both chimeric lysins) have been found to have effect against MRSA (Yang et al., 2014(Yang et al., , 2017. Both studies showed a large percentage of biofilm mass reduction when treated with chimeric lysin ClyH or ClyF. ...
... procedures of Hong (Yang et al. 2013). Twenty female mice from Institute for Cancer Research (ICR), aged 4 weeks, were used for intraperitoneal (i.p.) inoculation with different concentrations of Streptococcus suis HA9801 strain to determine the minimum lethal dose (MLD) of PlyARI within 3 days. ...