Yang Yang’s research while affiliated with Guizhou Academy of Agricultural Sciences and other places

This study aimed to investigate the impact of dietary soybean oil and probiotics on goat meat quality, total conjugated linoleic acids (TCLA) concentration, and nutritional quality indicators of goats. Thirty‐six male crossbred goats (Anglo‐Nubian♂× Thai native♀), weighing 18.3 ± 2.7 kg, were selected and randomly assigned to six groups in a 2 × 3 factorial design, with six replicates per group. The soybean oil supplementation levels were 25 and 50 g/kg, while the probiotic supplementation levels were 0, 2.5, and 5.0 g/h/day. The results showed that supplementing the diet with 50 g/kg soybean oil significantly improved the average daily gain (ADG) (p = 0.02) and carcass yield (p = 0.05), while reducing the feed conversion ratio (p = 0.05). Additionally, the addition of 2.5 g/h/day of probiotics significantly increased dry matter intake (p(L) = 0.05, p(Q) = 0.03). Notably, supplementation with 50 g/kg soybean oil reduced the Warner–Bratzler shear force (p = 0.05) and a* (p = 0.01) values of the Longissimus thoracis et lumborum. However, 2.5 g/h/day of probiotics significantly improved (p(L) = 0.01, p(Q) = 0.04) the a* value of Longissimus thoracis et lumborum. Soybean oil supplementation at 50 g/kg increased the ether extract composition of Biceps brachii (p = 0.05) and Semimembranosus (p = 0.05). Additionally, it significantly increased TCLA content (p < 0.01) and reduced the n−6/n−3 ratio (p < 0.01). Interestingly, the supplementation of 5.0 g/h/day probiotics significantly reduced the thrombogenic index (p = 0.03). Moreover, supplementing with 50 g/kg soybean oil (p = 0.03) and 5.0 g/h/day probiotics significantly improved the nutritive value index of goat muscle. Collectively, the findings suggest that the optimal supplementation levels of probiotics and soybean oil are 2.5 g/h/day and 50 g/kg, respectively. These levels have a more pronounced effect on improving the growth performance of growing goats, increasing CLA content, and enhancing meat quality.

This study aimed to reveal the effect of traditional Chinese herbal medicine residues (TCHMR) on growth performance, hematology, ruminal microbiota, and economic benefits of Guizhou black male goats through the fermented total mixed ration (FTMR) diet technique. A total of 22 Guizhou black male goats with an initial weight of 21.77 ± 0.85 kg were randomly divided into 2 groups (n = 11), with 11 goats in each group. The control group (CON) was fed a traditional total mixed ration (TMR) diet without the TCHMR. The TCHMR group was fed an FTMR diet containing 40%TCHMR. Compared with the CON group, the results showed that the incorporation of TCHMR into goat diets reduced feeding costs and Feed conversion ratio (FCR). On the contrary, it improved (P < 0.01) feed quality, apparent digestibility of Dry matter (DM), Crude Protein (CP), Neutral detergent fiber (NDF), average daily gain (ADG), and dry matter intake. Interestingly, TCHMR also reduced (P < 0.01) acetate levels in the rumen of goats. Supplementally, TCHMR significantly increased (P < 0.01) the levels of GH, IgM, IgA (p < 0.05), and IFN-γ (P < 0.05), while significantly reducing (P < 0.01) the levels of IL-6, ALT, and AST in serum. Notably, at the phylum level, TCHMR significantly reduced (P < 0.01) the abundance of Bacteroidota and increased (P < 0.01) the abundance of Firmicutes. Moreover. at the genus level, TCHMR significantly reduced (P < 0.01) the abundance of Prevotella, F082, and Bacteroidales_RF16_group, while Muribaculaceae, Proteus, Lachnospiraceae_ND3007_group, and Ruminococcus were increased (P < 0.01). In conclusion, our current findings indicated that 40% TCHMR improved feed quality and the apparent digestibility of nutrients. Additionally, 40% TCHMR improved the growth performance and immunity of Guizhou black male goats, while also reorganizing the composition of ruminal microbiota. So far, under the conditions of this experiment, we have not found any negative effects of 40% TCHMR on goats. This study will be a new idea for developing feed resources, which will reduce environmental pollution and the cost of animal husbandry.

The aim of this study is to explore the genetic characteristics of Guizhou Black goats and Meigu goats and their relationship to reproductive performance through population structure analysis, genetic diversity assessment, and selection signal analysis. Blood samples of 19 Guizhou Black goats and 11 Meigu goats were collected for whole-genome high-throughput sequencing. Using PCA and ADMIXTURE analyses, their population structure and genetic relationships were revealed. Further genetic diversity analysis showed that although there is significant population differentiation, the levels of genetic diversity are similar. Subsequently, these goats were categorized into high-yield and low-yield groups based on their litter sizes, with 15 goats in each group. Then, a selection signal analysis was performed using FST and π ratios for 33,563 SNP loci. The results identified six candidate genes, including KCNIP4, GFRA2, and DGKH, which are significantly associated with high litter performance. These findings enhanced our understanding of the genetic characteristics and population structure of Guizhou Black goats and Meigu goats. Moreover, they provide an important theoretical foundation and scientific basis for further breeding improvements.

Introduction The primary objective of the current study was to evaluate the effects of Flammulina velutipes mushroom residue (FVMR) in a fermented total mixed ration (FTMR) diet on the fattening effect and rumen microorganisms in Guizhou black male goats. Methods A total of 22 Guizhou black male goats were allocated into two groups using the Randomized Complete Block Design (RCBD) experimental design. The average initial weight was 22.41 ± 0.90 kg and with 11 goats in each group. The control group (group I) was fed the traditional fermentation total mixed ration (FTMR) diet without FVMR. Group II was fed the 30% FVMR in the FTMR diet. Results The results showed that compared with group I, the addition of FVMR in the goat diet could reduce the feed cost and feed conversion ratio (FCR) of group II (p < 0.01). Notably, the apparent digestibility of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and dry matter (DM) were higher in group II (p < 0.01). The levels of growth hormone (GH), immunoglobulin A (IgA), and immunoglobulin M (IgM) in group II were higher than that of group I (p < 0.01), which the level of glutamic oxalacetic transaminase (ALT) and interleukin-6 (IL-6) was noticeably lower than that of group I (p < 0.01). 30% FVMR in FTMR diets had no effect on rumen fermentation parameters and microbial composition at the phylum level of Guizhou black male goats (p > 0.05). However, at the genus level, the relative abundance of bacteroidal_bs11_gut_group, Christensenellaceae_R-7_group and Desulfovibrio in group II was lower than in group I (p < 0.05), and the relative abundance of Lachnospiraceae_ND3007_group was higher than in group I (p < 0.01). Discussion In conclusion, the results of the current study indicated that 30% FVMR in the FTMR diet improves rumen fermentation and rumen microbial composition in Guizhou black male goats, which improves growth performance, apparent digestibility, and immunity.

This study was conducted to examine the effect of a mulberry leaf total mixed ration (TMR) diet on growth performance, apparent digestibility, meat quality and the expression of related meat-quality genes (ADSL, H-FABP) in crossbred black goats. Forty-four Guizhou crossbred black goats (Nubian black goat ♂ × Guizhou black goat ♀), weighing 33.43 ± 0.55 kg, were chosen. The goats were randomly divided into four groups, with 11 test replicates in each group. Group I was the control group and fed with the traditional feeding method of roughage and concentrate supplement without adding mulberry leaf. Group II was fed with a 40% mulberry leaf pellet TMR diet. Group III was fed with a freshly processed 40% mulberry leaf TMR diet. Group IV was fed with a 40% mulberry leaf fermented total mixed rations (FTMR) diet. The results showed that the average daily gain (ADG) of group II was significantly higher than that of group I and III (p < 0.05). The apparent digestibility of group II of ether extract (EE) and neutral detergent fiber (NDF) was significantly higher than that of group I (p < 0.05), and the apparent digestibility of dry matter (DM) and crude protein (CP) was significantly higher than that of group I (p < 0.01). Compared with group I, meat in group II had lower meat color lightness (L*) and yellowness (b*) values (p < 0.01) in the Longissimus thoracis et lumborum. The shear force of group II was significantly lower than that of group I (p < 0.05). The total fatty acids (TFA) of group II was significantly higher than that of groups I and III (p < 0.05), but the total saturated fatty acids (SFA) of group II was significantly lower that than of group I (p < 0.01). Subsequently, the Unsaturated fatty acids (USFA), Monounsaturated fatty acids (MUFA), and Polyunsaturated fatty acids (PUFA) of group II were significantly higher than those in group I (p < 0.01). The contents of total amino acids (TAA), total essential amino acids (EAA), total non-essential amino acids (NEAA) and total of major fresh-tasting amino acids (DAA) of groups II, III and IV were significantly higher than those of group I (p < 0.05), as well as the contents of IMP (p < 0.01). The expression of the H-FABP gene in the arm triceps of group II was significantly higher than that of groups I, III and IV (p < 0.05). The expression of the ADSL gene in the Longissimus thoracis et lumborum and biceps femoris of group II was significantly higher than that of group I (p < 0.05). Collectively, the results of the current study indicated that the mulberry leaf TMR diet improved the growth performance, apparent digestibility and expression of related meat-quality master genes (ADSL, H-FABP) in crossbred black goats, which promoted the deposition of intramuscular fat (IMF) and inosinic acid (IMP) and improved the composition of fatty acids and amino acids in the muscles.

To study the effects of the seleno-chitosan on daily gain, wool yield, and blood parameters in the Chinese merino sheep in the selenium (Se)-deficient pastures. The samples of soils, forages, and tissues had been collected in the Southern Xinjiang of Northwest China. Our findings indicated that the Se contents in soils and forages from affected pastures were remarkably lower than those unaffected by the pastures (P < 0.01). The Se contents in the blood and the wool from affected Chinese merino sheep were extremely lower than those from healthy sheep. Meanwhile, the values of Hb, PCV, and PLT in affected sheep were significantly decreased (P < 0.01). The yield of wool and the growth rate in affected sheep were also remarkably reduced (P < 0.01). The affected Chinese merino sheep were orally treated by seleno-chitosan for 150 days; the Se contents in blood were remarkably increased and reached the healthy range on day 5. The blood parameters soon recovered to a healthy range on day 10. The growth rate of sheep in the treated group was remarkably higher than that in the control animals. The yield of the wool was also significantly increased in the treated group. Consequently, the Se-deprived environment caused a threat to daily gain, wool yield, and blood parameters in the Chinese merino sheep. The seleno-chitosan could not only markedly increase the Se contents blood contents, but also significantly increase the production performance (daily gain and wool yield), and release the symptoms of anemia in the Se-deprived animal.

The study was implemented for exploring influences of nano-K2MoO4 on the daily gain and antioxidant function of Chinese merino sheep in the native pasture, Xinjiang Uygur Autonomous Region, Northwest of China. Eighty of the sheep, weight of (45.56 ± 2.35) kg, were randomly distributed to the tested areas for 90 days, 20 sheep/group. The findings showed that the contents of Mo and N in the forage of applying nano-K2MoO4 were extremely higher than those in the control pastures (P <0.01). The daily gain in the fertilized groups were remarkably lower than that in the control group (P <0.01). The harvest of forage in the fertilized pastures were significantly higher than those in control (P <0.01). The contents of Mo in blood and liver in grazing the Chinese merino sheep were extremely higher than those from the control group (P <0.01). The contents of Cu in blood and liver in grazing the Chinese merino sheep were extremely lower than those in the unfertilized pastures (P <0.01). The levels of hemoglobin (Hb), blood platelet (PLT), and erythrocyte count (RBC) in animals from the fertilized pastures were extremely lower than those in the control pastures (P < 0.01). The activities of SOD, GSH-Px, and CAT in serum were significantly lower than those in group C. The serum MDA levels were significantly higher than those in the control group (P < 0.01). In conclusion, the application of nano-K2MoO4 in pastures can greatly improve the yield of forage, but strikingly decreased the daily gain and antioxidant function in grazing the Chinese merino sheep.

The aims of this study were to investigate the impact of different levels of nano-potassium molybdate (nano-K2MoO4) fertilization on the copper (Cu) metabolism in grazing the Pishan red sheep in the natural pasture. The fertilization and grazing experiments were conducted on the Pishan farm in Southern Xinjiang, China. The natural pastures of 16 hm2 were randomly divided into four groups (4 hm2/group), consisting of group C (no fertilized), group I, group II, and group III. The fertilizing amount of Mo from nano-K2MoO4 was 0, 7, 8, and 9 kg/hm2 for group C, group I, group II, and group III, respectively. The 40 Pishan red sheep were randomly distributed to the tested pastures for 90 days, and the 10 sheep/group. The results showed that the contents of Mo and N in forage from the fertilized pastures were extremely increased (P < 0.01). The yield and dry matter digestibility of forage in fertilized pastures were significantly higher than those in no fertilized pasture (P < 0.01). The values of crude protein (CP) and crude fat (EE) in forage from fertilized pastures were significantly increased (P < 0.01). The Mo contents in the blood and liver in the Pishan red sheep from fertilized pastures were greatly increased (P < 0.01). The Cu contents in the blood and liver in the Pishan red sheep from the fertilized pastures were greatly decreased (P < 0.01). The activities of serum superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in group I, group II, and group III were extremely lower than those in group C (P < 0.01), and the contents of serum malondialdehyde (MDA) in group I, group II, and group III were greatly higher than those from group C (P < 0.01). In summary, the application of nano-K2MoO4 improved the nutritive values and the yield of forage, but overuse will remarkably reduce the Cu contents of blood and greatly interfere with the Cu metabolism, leading to the Cu deficiency and low antioxidant capacity in grazing the ruminants.