Y. Hirota's research while affiliated with Nagoya University and other places

Publications (5)

Article
Full-text available
A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilit...
Article
The influence ofKlebsiella oxytoca andEnterobacter cloacae inoculation on dinitrogen fixation by the rice-bacteria association was examined in pots in a greenhouse. For inoculation,K. oxytoca NG13 isolated from a Japan paddy soil,E. cloacae E26 isolated from a China soil and following three strains were employed.K. oxytoca NG1389 is a mutant from N...
Article
Nâ fixation by Klebsiella oxytoca strain NG13, isolated from the rhizosphere of a rice (Oryza sativa L.) plant, in association with Indica type rice strain, C5444 is reported. The CâHâ-reduction assays, biomass measurements, N content determinations, and ¹⁵N isotopic dilution assays indicated that inoculation of C5444 with NG13 resulted in a 6%...
Article
THE discovery of nitrogen-fixing activity associated with the rhizosphere of non-leguminous plants such as tropical grasses, rice and maize has provoked considerable interest1–9. Discrepancies in the measurements of this nitrogen-fixing activity by the acetylene reduction assay in different conditions prompted us to develop a modified assay system....

Citations

... In rhizosphere soils where root exudates are excreted as carbon and energy sources, the N 2 -fixing activity by free-living diazotrophs such as Beijerinckia, Azotobacter, and Klebsiella is enhanced. (Yoo et al. 1986) Enterobacter cloacae (Ladha et al. 1983) Endophytic, in the root tissues Azospirillum spp. (Hill et al. 1983) Alcaligenes faecalis (You and Zou 1989) Burkholderia spp. ...
... Significant differences in rice-associated ARA have been found among five rice varieties in both the wet and dry seasons (Ladha et al., 1986). Sano et al. (1981) found that the ARA of cultivated rice cultivars was higher than that of wild cultivars. By measuring the natural abundance of 15 N, Watanabe et al. (1987) observed statistically significant differences in grain 15 N abundance among rice varieties grown during the wet season, but not the dry season. ...
... Biological N 2 fixation is also affected by root C input. Higher N 2 fixation rates have been observed during early rice growth when the root exudation is most active (Yoshida and Ancajas, 1973;Ladha et al., 1986) and in the rhizosphere of cultivars with large root systems (Hirota et al., 1978). Additionally, increased abundance and activity of diazotrophs were observed in free-air CO 2 -enrichment experiments (Guo et al., 2015;Hoque et al., 2001). ...
... The loss of chlorophylls and carotenoids are the primary responses associated with the unavailability of Fe in plants (Belkhodja et al., 1998). Inoculation studies have been commonly used to elucidate the plant probiotic features of microbes (Fujii et al., 1987;Radzki et al., 2013;Young et al., 2013). Sharma & Johri (2003) proved that the siderophore-producing Pseudomonas strain GRP3 significantly increases chlorophyll contents and decreases chlorotic symptoms in Vigna radiate. ...
... The fhuF gene region was cloned on a 3-kb EcoRV DNA fragment from λ phage 8D1 (672) of the Kohara collection [12] in the high-copy-number vector pSU18 and recloned into vector pT7-5, resulting in plasmid pKF7572. To obtain an insert coding [12] Vector pT7Ϫ5 Amp R phage T7 Φ10 promoter [16] pACYC184 ...