Xixun Yu’s research while affiliated with Sichuan University and other places

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Publications (81)


Photocrosslinked methacrylated carboxymethyl chitosan/oxidized locust bean gum double network hydrogel for cartilage repair
  • Article

October 2023

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10 Reads

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8 Citations

Journal of Materials Chemistry B

Can Cheng

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Xu Peng

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Yihao Luo

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[...]

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Xixun Yu

Repairing articular cartilage defects is a great challenge due to the poor self-regenerative capability of cartilage. Inspired by active substances found in the natural cartilage extracellular matrix, we used methacrylated carboxymethyl chitosan (MA-CMCS) and oxidized locust bean gum (OLBG) as the hydrogel backbone, and prepared a photocrosslinked dual network hydrogel containing allicin and decellularized cartilage powder (DCP). The rheological, swelling and water retention capacities of MA-CMCS@OLBG-Allicin/DCP (MCOAC) hydrogels were investigated to confirm the successful preparation of hydrogels suitable for cartilage repair. The MCOAC hydrogels showed good antibacterial ability to kill S. aureus and E. coli and anti-inflammatory properties due to the introduction of allicin. Furthermore, MA-CMCS@OLBG-Allicin/DCP hydrogels presented good cytocompatibility due to the addition of DCP, which could promote chondrocyte proliferation and promote the differentiation of BMSCs to chondrocytes. Further studies in vivo demonstrated that the DCP-contained MCOAC hydrogel exhibited superior performance in promoting cartilage tissue growth and wound healing in articular cartilage defects. Thus, the MCOAC hydrogel is a promising cartilage repair hydrogel with potential for clinical use.



X-ray images in the 6th week and 20th week.
Corrigendum: Research on the inhibition for aseptic loosening of artificial joints by Sr-doped calcium polyphosphate (SCPP)in vivo(2021Biomed. Mater.16065017)
  • Article
  • Publisher preview available

September 2023

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6 Reads

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dECM based dusal-responsive vascular graft with enzyme-controlled adenine release for long-term patency

May 2023

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16 Reads

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4 Citations

International Journal of Biological Macromolecules

Rapid occlusion is the culprit leading to implantation failure of biological blood vessels. Although adenosine is a clinical-proven drug to overcome the problem, its short half-life and turbulent burst-release limit its direct application. Thus, a pH/temperature dual-responsive blood vessel possessed controllable long-term adenosine secretion was constructed based on acellular matrix via compact crosslinking by oxidized chondroitin sulfate (OCSA) and functionalized with apyrase and acid phosphatase. These enzymes, as adenosine micro-generators, controlled the adenosine release amount by "real-time-responding" to acidity and temperature of vascular inflammation sites. Additionally, the macrophage phenotype was switched from M1 to M2, and related factors expression proved that adenosine release was effectively regulated with the severity of inflammation. What's more, the ultra-structure for degradation resisting and endothelialization accelerating was also preserved by their "double-crosslinking". Therefore, this work suggested a new feasible strategy providing a bright future of long-term patency for transplanted blood vessels.


Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory

April 2023

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8 Reads

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3 Citations

International Journal of Biological Macromolecules

Acellular porcine aorta (APA) is an excellent candidate for an implanted scaffold but needs to be modified with appropriate cross-linking agent to increase its mechanical property and storage time in vitro as well as to give itself some bioactivities and eliminate its antigenicity for acting as a novel esophageal prosthesis. In this paper, a polysaccharide crosslinker (oxidized chitosan, OCS) was prepared by oxidizing chitosan using NaIO4 and further used to fix APA to prepare a novel esophageal prosthesis (scaffold). And then the surface modification with dopamine (DOPA) and strontium-doped calcium polyphosphate (SCPP) were performed one after another to prepare DOPA/OCS-APA and SCPP-DOPA/OCS-APA to improve the biocompatibility and inhibit inflammation of the scaffolds. The results showed that the OCS with a feeding ratio of 1.5:1.0 and a reaction time of 24 h had a suitable molecular weight and oxidation degree, almost no cytotoxicity and good cross-linking effect. Compared with glutaraldehyde (GA) and genipin (GP), OCS-fixed APA could provide a more suitable microenvironment for cell proliferation. The vital cross-linking characteristics and cytocompatibility of SCPP-DOPA/OCS-APA were evaluated. Results suggested that SCPP-DOPA/OCS-APA exhibited suitable mechanical properties, excellent resistance to enzymatic degradation/acid degradation, suitable hydrophilicity, and the ability to promote the proliferation of Human normal esophageal epithelial cells (HEECs) and inhibit inflammation in vitro. In vivo tests also confirmed that SCPP-DOPA/OCS-APA could diminish the immunological response to samples and had a positive impact on bioactivity and anti-inflammatory. In conclusion, SCPP-DOPA/OCS-APA could act as an effective, bioactive artificial esophageal scaffold and be expected to be used for clinical in the future.


Cascade Regulation of the Proliferation, Recruitment, and Differentiation of Stem Cells to Prevent Aseptic Loosening by GNPs/ECPP Particles Responding to Macrophages: In Vitro and In Vivo

March 2023

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8 Reads

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2 Citations

ACS Biomaterials Science & Engineering

Modulating both inflammation and stem cells by designing an artificial joint material to obtain the continuous prevention and control on aseptic loosening (AL) is a novel strategy. In this paper, graphene/europium-doped calcium polyphosphate (GNPs/ECPP) particles were obtained by ultrasound method and spark plasma sintering (SPS) method. The prepared particles were used to modulate the inflammatory response and further obtain cascade regulation on the proliferation, recruitment, and differentiation of stem cells. The results showed that particles obtained by SPS had a stronger effect on promoting the proliferation and differentiation of stem cells, while by ultrasound method more stem cells were recruited. Besides, the graphene and Eu3+ contained in the particles obtained by SPS method could effectively play a synergistic role on the differentiation of stem cells. In vivo experiment results demonstrated that the composite particles effectively suppress the inflammatory response, recruit stem cells, and prevent AL by inhibiting the secretion of inflammatory factors.


Cross-linking porcine peritoneum by oxidized konjac glucomannan: a novel method to improve the properties of cardiovascular substitute material

February 2023

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71 Reads

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4 Citations

Journal of Leather Science and Engineering

The use of natural polysaccharide crosslinkers for decellularized matrices is an effective approach to prepare cardiovascular substitute materials. In this research, NaIO 4 was applied to oxidize konjac glucomannan to prepare the polysaccharide crosslinker oxidized konjac glucomannan (OKGM). The as-prepared crosslinker was then used to stabilize collagen-rich decellularized porcine peritoneum (DPP) to construct a cardiovascular substitute material (OKGM-fixed DPP). The results demonstrated that compared with GA-fixed DPP and GNP-fixed DPP, 3.75% OKGM [1:1.5 (KGM: NaIO 4 )]-fixed DPP demonstrated suitable mechanical properties, as well as good hemocompatibility, excellent anti-calcification capability, and anti-enzymolysis in vitro. Furthermore, 3.75% OKGM [1:1.5 (KGM: NaIO 4 )]-fixed DPP was suitable for vascular endothelial cell adhesion and rapid proliferation, and a single layer of endothelial cells was formed on the fifth day of culture. The in vivo experimental results also showed excellent histocompatibility. The current results demonstrted that OKGM was a novel polysaccharide cross-linking reagent for crosslinking natural tissues featured with rich collagen content, and 3.75% OKGM [1:1.5 (KGM: NaIO 4 )]-fixed DPP was a potential cardiovascular substitute material. Graphical Abstract


Dialdehyde xanthan gum and curcumin synergistically crosslinked bioprosthetic valve leaflets with anti-thrombotic, anti-inflammatory and anti-calcification properties

February 2023

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11 Reads

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12 Citations

Carbohydrate Polymers

Currently commercial glutaraldehyde (GA)-crosslinked bioprosthetic valve leaflets (BVLs) suffer from thromboembolic complications, calcification, and limited durability, which are the major stumbling block to wider clinical application of BVLs. Thus, developing new-style BVLs will be an urgent need to enhance the durability of BVLs and alleviate thromboembolic complications. In this study, a quick and effective collaborative strategy of the double crosslinking agents (oxidized polysaccharide and natural active crosslinking agent) was reported to realize enhanced mechanical, and structural stability, excellent hemocompatibility and anti-calcification properties of BVLs. Dialdehyde xanthan gum (AXG) exhibiting excellent stability to heat, acid-base, salt, and enzymatic hydrolysis was first introduced to crosslink decellularized porcine pericardium (D-PP) and then curcumin with good properties of anti-inflammatory, anti-coagulation, anti-liver fibrosis, and anti-atherosclerosis was used to synergistically crosslink and multi-functionalize D-PP to obtain AXG + Cur-PP. A comprehensive evaluation of structural characterization, hemocompatibility, endothelialization potential, mechanical properties and component stability showed that AXG + Cur-PP exhibited better anti-thrombotic properties and endothelialization potential, milder immune responses, excellent anti-calcification properties and enhanced mechanical properties compared with GA-crosslinked PP. Overall, this cooperative crosslinking strategy provides a novel solution to achieve BVLs with enhanced mechanical properties and excellent anti-coagulation, anti-inflammatory, anti-calcification, and the ability to promote endothelial cell proliferation.



Sulfonated, oxidized pectin-based double crosslinked bioprosthetic valve leaflets for synergistically enhancing hemocompatibility and cytocompatibility and reducing calcification

September 2022

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11 Reads

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4 Citations

Journal of Materials Chemistry B

Clinically frequently-used glutaraldehyde (GA)-crosslinked bioprosthetic valve leaflets (BVLs) are still curbed by acute thrombosis, malignant immunoreaction, calcification, and poor durability. In this study, an anticoagulant heparin-like biomacromolecule, sulfonated, oxidized pectin (SAP) with a dialdehyde structure was first obtained by modifying citrus pectin with sulfonation of 3-amino-1-propane sulfonic acid and then oxidating with periodate. Notably, a novel crosslinking approach was established by doubly crosslinking BVLs with SAP and the nature-derived crosslinking agent quercetin (Que), which play a synergistic role in both crosslinking and bioactivity. The double crosslinked BVLs also presented enhanced mechanical properties and enzymatic degradation resistance owing to the double crosslinking networks formed via CN bonds and hydrogen bonds, respectively, and good HUVEC-cytocompatibility. The in vitro and ex vivo assay manifested that the double-crosslinked BVLs had excellent anticoagulant and antithrombotic properties, owing to the introduction of SAP. The subcutaneous implantation also demonstrated that the obtained BVLs showed a reduced inflammatory response and great resistance to calcification, which is attributed to quercetin with multiple physiological activities and depletion of aldehyde groups by hydroxyl aldehyde reaction. With excellent stability, hemocompatibility, anti-inflammatory, anti-calcification, and pro-endothelialization properties, the obtained double-crosslinked BVLs, SAP + Que-PP, would have great potential to substitute the current clinical GA-crosslinked BVLs.


Citations (58)


... Glutaraldehyde (GA) cross-linked collagen improved collagen's thermal stability and anti-enzyme activity. Nonetheless, GA posed potential risks of cytotoxicity and calcification [17,18]. The process of cross-linking involving 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) bolstered the mechanical resilience of collagen. ...

Reference:

Engineering a durable BDDE cross-linked collagen filler for skin rejuvenation
Double Crosslinking decellularized bovine pericardium of Dialdehyde Chondroitin Sulfate and Zwitterionic Copolymer for Bioprosthetic Heart Valves with Enhanced Antithrombogenic, Anti-inflammatory and Anti-calcification Properties
  • Citing Article
  • March 2024

Journal of Materials Chemistry B

... The incorporation of MoS 2 into composite materials enhances the mechanical strength of the scaffolds, thus offering crucial structural reinforcement for tissue regeneration. 18 The studies have proven the composites of polycaprolactone-MoS 2 , 19 polycaprolactone-MoS 2 nanofiber, 20 polyacrylonitrile-MoS 2 , 21 MoS 2 nanosheet-polycaprolactone/zein, 18 MoS 2 -hydroxyapatite, 22 alginate-casein-MoS 2 , 23 polycaprolactone-polylactide-co-glycolide-bioactive glass-MoS 2 nanoparticles, 24 MoS 2 -metformin, 25 poly(lactic-coglycolic) acid-simvastatin-mesoporous bioactive glass-MoS 2 , 26 and MoS 2 -bioactive borosilicate glass 27 have shown better biocompatibility, biodegradability, enhanced alkaline phosphatase (ALP), and increased bone mineralization when compared with the control. Hence, the addition of MoS 2 in the composite system significantly increases the overall biocompatibility of the scaffold. ...

3D Molybdenum Disulfide Nanospheres Loaded with Metformin to Enhance SCPP Scaffolds for Bone Regeneration
  • Citing Article
  • December 2023

ACS Applied Materials & Interfaces

... Many hydrogels incorporate bioactive molecules that promote cell proliferation and differentiation, such as growth factors (Jain et al., 2019), or provide physical support to facilitate new cartilage formation (Yang et al., 2023). For example, Cheng et al. (2023) developed a double-network hydrogel with antibacterial and anti-inflammatory properties, enhancing cartilage repair by incorporating allicin and decellularized cartilage powder. Similarly, Chen et al. (2024) designed a nanocomposite hydrogel with poly (gallic acid)-manganese (PGA-Mn) nanoparticles, which strengthens the hydrogel while scavenging ROS, protecting chondrocytes from oxidative stress. ...

Photocrosslinked methacrylated carboxymethyl chitosan/oxidized locust bean gum double network hydrogel for cartilage repair
  • Citing Article
  • October 2023

Journal of Materials Chemistry B

... Decellularization is an emerging biomaterial preparation method in tissue engineering [17], which involves the removal of cellular components from biological tissues or organs to obtain a protein matrix for cell growth [18], which is ideal raw material for the preparation of wound dressings [19]. In many current studies on tissue regeneration, decellularized matrices of cardiac origin are attractive because they retain key molecules for wound healing, such as collagen, elastin, fibronectin and pro-angiogenic factors (ANG-1, ANG-3, CCN1, FGF-1, FGF-2, leptin, MMP-9, NOV, SDF-1 and VEGF -β) [20], which not only accelerates wound re-epithelialization to accelerate wound closure [21], but also improves healing quality by increasing collagen deposition and maturation. ...

A synergistic strategy of dual-crosslinking and loading intelligent nanogels for enhancing anti-coagulation, pro-endothelialization and anti-calcification properties in bioprosthetic heart valves
  • Citing Article
  • October 2023

Acta Biomaterialia

... The result is consistent with the literature. 28,29 The C2-C3 linkage was cleaved in the N-acetyl-glucosamine unit (GlcN) and dialdehyde was formed. ...

Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory
  • Citing Article
  • April 2023

International Journal of Biological Macromolecules

... The hemocompatibility of valve prostheses is crucial for their clinical application. Previous studies have employed numerous modification strategies to mitigate the biofouling of blood constituents and enhance the hemocompatibility of valves [36][37][38][39]. In the present study, we modified dECM with an RBCM coating to enhance its hemocompatibility. ...

Dialdehyde xanthan gum and curcumin synergistically crosslinked bioprosthetic valve leaflets with anti-thrombotic, anti-inflammatory and anti-calcification properties
  • Citing Article
  • February 2023

Carbohydrate Polymers

... The most common method to modify and introduce aldehyde or ketone groups into polysaccharides is via oxidative cleavage of vicinal diols driven by sodium periodate (NaIO 4 ) [117,118]. Muhammad et al. [88] evaluated the effect of reaction time and sodium periodate (NaIO 4 ) concentration on the degree of oxidation of alginate and hyaluronic acid. These polysaccharides can yield in situ gelling hydrogels to be used in drug delivery systems by imine bond formation. ...

Cross-linking porcine peritoneum by oxidized konjac glucomannan: a novel method to improve the properties of cardiovascular substitute material

Journal of Leather Science and Engineering

... Besides, DCDs possess abundant reducing groups, which can scavenge ROS and promote the phenotype switching of macrophages from M1-type to M2-type, which is favorable for suppressing inflammatory responses. 80 Manipulation of BMSCs differentiation is becoming an essential aspect of bone regeneration. Shao and his colleagues demonstrated that CDs can promote the osteogenic differentiation of BMSCs for the first time. ...

A novel multifunctional dexamethasone carbon dots synthesized by one-pot green method for anti-inflammatory, osteogenesis and osteoimmunomodulatory in bone regeneration
  • Citing Article
  • September 2022

Biomaterials Science

... It has been found that RES stimulates the production of collagen III and improves the proliferation and migration of human gingival fibroblasts (72). Oxidized NAR exhibited good cytocompatibility and could promote the secretion of growth factors by fibroblasts (73). ...

Feasibility study of oxidized naringin as a novel crosslinking agent for crosslinking decellularized porcine A chilles tendon and its potential application for anterior cruciate ligament repair
  • Citing Article
  • August 2022

Journal of Biomedical Materials Research Part A

... TA increases the mechanical strength, decreases the excessive swelling rate, and promotes the adherence of the hydrogel to various substrates. According to Cheng et al., the combination of PVA and agar loaded with tannic acid presented promising characteristics in terms of hemostatic, antibacterial activity, cytocompatibility, and rapid wound healing [34]. Finally, the composite hydrogel developed in this study can effectively be applied in crucial areas such as artificial cartilage. ...

An agar-polyvinyl alcohol hydrogel loaded with tannic acid with efficient hemostatic and antibacterial capacity for wound dressing
  • Citing Article
  • August 2022

Food & Function