Xiping Luo’s research while affiliated with Abbott Northwestern Hospital and other places

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Publications (26)


Image of MRI (A) on the coronal T2‐weighted sequence, the left ovarian tumor (indicated by the white arrow) exhibits cystic and solid changes with mixed high and low T2 signals. Similarly, the right ovarian tumor (indicated by *) also displays cystic and solid changes with mixed high and low T2 signals. The uterus (indicated by the black arrow) is compressed and shifted toward the lower right. (B) Axial T2‐weighted sequence shows cystic and solid changes in the left ovarian tumor (indicated by the white arrow), with mixed high and low T2 signals. Similarly, the right ovarian tumor (*) also exhibits cystic and solid changes with mixed high and low T2 signals. (C) Coronal T1‐weighted enhanced sequence demonstrates significant and inhomogeneous enhancement in the solid portion of the left ovarian tumor (indicated by the white arrow), while the cystic portion shows no significant enhancement. The solid part of the right ovarian tumor (*) also exhibits significant and inhomogeneous enhancement, with no apparent enhancement in the cystic portion. (D) Axial T1‐weighted enhanced sequence reveals significant and inhomogeneous enhancement in the solid part of the left ovarian tumor (indicated by the white arrow), while the cystic part does not show significant enhancement. Similarly, the solid portion of the right ovarian tumor (*) demonstrates significant and inhomogeneous enhancement, with no observable enhancement in the cystic part. The uterine muscle layer enhances uniformly.
Image of MRI on diffusion‐weighted imaging (DWI), the densely populated areas of tumor cells (indicated by the white arrow) exhibit high‐signal intensity, while the cystic and necrotic regions of the tumor (indicated by *) show low signal intensity.
Pathological tumor features (A) gross features: A large, irregular tumor with a grayish‐white solid cut surface accompanied by cystic changes and necrosis. (B–D) Hematoxylin–eosin (original magnification× 100): Microscopically, the tumor cells are arranged in solid sheets, sieve‐like patterns, glands, papillae, trabeculae, and adenoma‐like structures. Locally, the cytoplasm appears transparent with mucinous stroma. The nuclei are moderately atypical, and the number of mitoses is less than 5 per 10 high‐power fields (HPF).
Representative images of immunohistochemical stains. (A) Positive immunostaining for CK‐pan. (B) Positive immunostaining for Vimentin. (C) Positive immunostaining for CD10. (D) Negative immunostaining for a‐inhibin (original magnification× 100 [A–D]).
A Case Report of Giant Bilateral Wolffian Adnexal Tumor
  • Article
  • Full-text available

December 2024

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3 Reads

Ling Huang

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Yan Zhou

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Xiaoshan Hong

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[...]

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Xiaoli Sun

Background Wolffian adnexal tumor is a rare type of tumor that was first discovered and reported by Karim‐inejad in 1973. Wolffian adnexal tumor lacks specific clinical manifestations and its histological morphology is similar to various other tumors, making it highly prone to misdiagnosis. To enhance our understanding of this disease, we hereby report a case of Wolffian adnexal tumor diagnosed and treated in our hospital. Case It is the first report of a giant bilateral Wolffian tumor, with pleural effusion and pericardial effusion as the initial symptoms. Magnetic resonance imaging (MRI) suggested a huge lobulated mass (25.3 × 17.8 × 21.9 cm) in the mid‐lower abdomen and pelvis, involving both ovaries. A diagnosis of “ovarian malignancy” was made before the surgery. Hysterectomy, bilateral adnexectomy, and omentectomy were performed. Postoperative pathology revealed a bilateral Wolffian tumor. Postoperative chemotherapy with a taxol and cisplatin (TP) regimen was administered for six cycles. Follow‐up at 2 months postoperatively showed resolution of pericardial and pleural effusions, and there has been no recurrence during the 3‐year follow‐up period. Conclusion Wolffian adnexal tumor lacks specific clinical manifestations, and its prognosis is good after treatment.

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Comparison of LEEP surgical design in patients with different reproductive requirements No Fertility requirements Fertility requirements P-Value
The impact of individualized design of cervical LEEP surgery on endocervical margin status and disease prognosis

February 2024

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5 Reads

Aim This study aimed to explore the relationship between individualized design of cervical loop electrosurgical excision procedure (LEEP ) and endocervical margin status as well as prognosis of cervical lesions. Methods A total of 151 outpatients dignosed with cervical high-grade squamous intraepithelial lesion(HSIL)in Guangdong Women and Children Hospital from January 2015 to December 2019 were included in this study. From the perspective of a patient's fertility requirments, the colposcopist conducted individualized LEEP with them. The pathologic features of the endocervical margins, presence of cervical stenosis or adhesion, and HPV test results within 2 years after the surgery were documented. Results 1.Endocervical margin positive rate of LEEP is13.25%(20/151). There were significant differences observed in design of LEEP between the patients with different reproductive requirements, although no statistically difference was detected in endocervical margin positivity rates (P = 0.979)with them.2.Univariate logistic regression analysis revealed that cervical cytology (P = 0.040), the number of quadrants involved by acetowhite epithelium (P = 0.032), and lesion grade of biopsy (P = 0.028) were significantly associated with endocervical margin .Multivariate logistic regression analysis revealed that quadrants involved by acetowhite epithelium (P = 0.034) and the grade of biopsy(P = 0.020)were independent risk factors for positive endocervical margin. 3.HPV clearance rate is 84.77% in 2 years after surgery.There are no significant differences with different endocervical margin status and HPV recheck results (≤ 6 months, 7–12 months, and 12-24months) (P = 1.000, 0.984, 0.382);4.Approximately 58.9%, 80.1%, and 86.8% of lesions can be removed with cone lengths ≤ 1cm,≤1.5cm, and ≤ 2.0cm, respectively. Conclusion Individualized LEEP surgical design is feasible and effective in clinical practice.Women with fertility requirements will benefit even more.


m⁶A modification-related enzymes in mRNA and their major biological functions.
m⁵C, m¹A, ψ, and A-to-I modifications on RNA.
RNA modifications in gynecological cancer: current status and future directions

January 2024

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23 Reads

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1 Citation

Currently, more than 170 modifications have been identified on RNA. RNA modification mainly regulates RNA splicing, intracellular transport, degradation, translation, and stability. Gynecologic cancer (GC) mainly includes cervical cancer (CCA), ovarian cancer (OC), Endometrial cancer (EMC), among others, is the leading cause of cancer-related death. At present, there is still a lack of effective means to eradicate such diseases, so it is important to conduct more in-depth research on gynecological cancers. Numerous studies have shown that a series of epigenetic changes occur during the development of gynecologic cancer. This article reviews the latest findings on the functional significance of RNA modification in gynecologic cancer and discusses the therapeutic potential of RNA modification-related inhibitors in the treatment of gynecologic cancer.



MYL9 mRNA and protein expression in SCC tissues and cell lines. (a, b) Reverse transcription-quantitative polymerase chain reaction revealed higher MYL9 mRNA expression in SCC tissues than in peritumoral tissues and (c) Western blotting illustrated that MYL9 protein was expressed in SCC cells. MYL9, myosin light chain 9; SCC, squamous cervical cancer.
Transwell and Boyden assays demonstrated that MYL9 knockdown suppresses SCC cell migration and invasion. (a) Knockdown of MYL9 reduced the migration of SCC cells in the Transwell assay and (b) Knockdown of MYL9 reduced cell invasion in SCC cells in the Boyden assay (magnification, ×200; scale bar = 50 µm). MYL9, myosin light chain 9; SCC, squamous cervical cancer.
MYL9 promoted the progression of SCC by enhancing aerobic glycolysis. (a) MYL9 knockdown decreased the Lactate production of SCC. (b) MYL9 knockdown inhibited the expression of GLUT1, HK2, and LDHA and (c) MYL9 knockdown inactivated the JAK2/STAT3 pathway. MYL9, myosin light chain 9; SCC, squamous cervical cancer; GLUT1, glucose transporter 1; HK2, hexokinase II; LDHA, lactate dehydrogenase A.
The detailed information of 36 patients with SCC.
MYL9 promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis

November 2023

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16 Reads

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6 Citations

Objective This study explored the mechanism of squamous cervical cancer (SCC) progression. Methods Reverse transcription-quantitative polymerase chain reaction and western blotting were used to evaluate the expression of myosin light chain 9 ( MYL9) in SCC tissues and cell lines. Furthermore, Transwell and Boyden assays were used to assess the function of MYL9 in SCC progression. In addition, the levels of lactate and aerobic glycolysis were used to explore the detailed mechanism of MYL9 in SCC. Results The mRNA and protein levels of MYL9 were elevated in SCC tissues, and MYL9 knockdown inhibited the migration and invasion of SCC cell lines. A mechanistic study demonstrated that MYL9 promotes SCC migration and invasion by enhancing aerobic glycolysis and increasing the activity of the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway. Conclusions MYL9 was upregulated in SCC, and it enhanced JAK2/STAT3 pathway activity and promoted metastasis and glycolysis in SCC.



The construction and analysis of tricarboxylic acid cycle related prognostic model for cervical cancer

March 2023

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55 Reads

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5 Citations

Introduction: Cervical cancer (CC) is the fourth most common malignant tumor in term of in incidence and mortality among women worldwide. The tricarboxylic acid (TCA) cycle is an important hub of energy metabolism, networking one-carbon metabolism, fatty acyl metabolism and glycolysis. It can be seen that the reprogramming of cell metabolism including TCA cycle plays an indispensable role in tumorigenesis and development. We aimed to identify genes related to the TCA cycle as prognostic markers in CC. Methods: Firstly, we performed the differential expressed analysis the gene expression profiles associated with TCA cycle obtained from The Cancer Genome Atlas (TCGA) database. Differential gene list was generated and cluster analysis was performed using genes with detected fold changes >1.5. Based on the subclusters of CC, we analysed the relationship between different clusters and clinical information. Next, Cox univariate and multivariate regression analysis were used to screen genes with prognostic characteristics, and risk scores were calculated according to the genes with prognostic characteristics. Additionally, we analyzed the correlation between the predictive signature and the treatment response of CC patients. Finally, we detected the expression of ench prognostic gene in clinical CC samples by quantitative polymerase chain reaction (RT-qPCR). Results: We constructed a prognostic model consist of seven TCA cycle associated gene (ACSL1, ALDOA, FOXK2, GPI, MDH1B, MDH2, and MTHFD1). Patients with CC were separated into two groups according to median risk score, and high-risk group had a worse prognosis compared to the low-risk group. High risk group had lower level of sensitivity to the conventional chemotherapy drugs including cisplatin, paclitaxel, sunitinib and docetaxel. The expression of ench prognostic signature in clinical CC samples was verified by qRT-PCR. Conclusion: There are several differentially expressed genes (DEGs) related to TCA cycle in CC. The risk score model based on these genes can effectively predict the prognosis of patients and provide tumor markers for predicting the prognosis of CC.


Study population. LLETZ: large loop excision of the transformation zone; UE: uterus expiration; CIN: cervical intraepithelial neoplasia; ASCUS: atypical squamous cells of unknown significance; No CIN: no cervical intraepithelial neoplasia or normal cervix/cervicitis; HSIL: high-grade squamous intraepithelial lesion; LSIL: low-grade squamous intraepithelial lesion; CC: cervical cancer; hrHPV: HPV31, 33, 35, 39, 45 51, 52, 56, 58, 59, 66 and 68.
The PAX1 and SEPT9 methylation levels were determined by using a qMS-PCR assay. Methylation scores of every sample were calculated using the above formula. In the scatter plots, the lines represent the 25th median and 75th percentiles.
Comparison of PAX1 and SEPT9 methylation levels in different histological lesions.
Comparisons of methylated Septin 9 and PAX1 in different severities of cervical lesions.
PAX1 and SEPT9 methylation analyses in cervical exfoliated cells are highly efficient for detecting cervical (pre)cancer in hrHPV-positive women

February 2023

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49 Reads

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4 Citations

Studies have investigated PAX1 and SEPT methylation were closely associated with cervical cancer. For this study, we verified the expressions of PAX1 and SEPT9 methylation in 236 hrHPV women cervical exfoliated cells by using quantitative methylation-specific PCR and we further explored their diagnostic value in cervical (pre)cancer detection. Our results identified that the methylation rates and levels of PAX1 and SEPT9 increased with cervical lesion severity. For a diagnosis of cervical (pre)cancer, the area under the curve (AUC) of PAX1 methylation was 0.77 (95% CI 0.71–0.83) and the AUC of SEPT9 methylation was 0.86 (95% CI 0.81∼0.90). Analyses of the PAX1 and SEPT9 methylation statuses alone or combined with commonly used tests can efficiently identify cervical (pre)cancer. In particular, SEPT9 methylation might serve as an effective and powerful biomarker for the diagnosis of cervical (pre)cancer and as an alternative triage test in HPV-based cervical (pre)cancer screening programs. Impact Statement What is already known on this subject? This subject showed that PAX1 and SEPT9 methylation were closely associated with cervical cancer. The methylation rates and levels of PAX1 and SEPT9 increased with cervical lesion severity and reached a peak in cervical cancer exfoliated cells. We further assessed the diagnostic performances of PAX1 and SEPT9 methylation in cervical cancer screening. In detecting cervical (pre)cancer, the sensitivity values of PAX1 and SEPT9 methylation were up to 61.18% and 82.35%, respectively, and the specificity values of PAX1 and SEPT9 methylation were up to 95.36% and 86.75%, respectively. Moreover, the ROC curve analysis showed AUC values of 0.77 for PAX1 methylation and 0.86 for SEPT9 methylation tests, which were significantly superior to other commonly used tests. These findings suggest that PAX1 and SEPT9 methylation detection may have great clinical potential in cervical cancer screening. What the results of this study add? The rates and levels of PAX1 and SEPT9 methylation increased with the severity of the cervical lesions. For a diagnosis of cervical (pre)cancer, the area under the curve (AUC) of PAX1 methylation was 0.77 (95% CI 0.71–0.83), and the sensitivity and specificity values were 61.18% and 95.36%, respectively. The AUC value of the SEPT9 methylation was 0.86 (95% CI 0.81 ∼ 0.90), and the sensitivity and specificity values were 82.35% and 86.75%, respectively. Compared with the various tests we conducted, the PAX1 methylation showed the highest specificity (95.36%), and the SEPT9 methylation demonstrated the highest accuracy(86.00%). What the implications are of these findings for clinical practice and/or further research? The methylation levels of PAX1 and SEPT9 had a certain predictive effect on the severity of cervical lesions in hrHPV-positive women. In addition, SEPT9 methylation analysis performs better than PAX1 methylation analysis and commonly used tests in cervical exfoliated cells for detecting cervical (pre)cancer in hrHPV-positive women. SEPT9 methylation analysis merits consideration as an effective and objective, alternative triage test in HPV-based cervical (pre)cancer screening programs.


Methylation scores median of Septin9 with increasing severity of cervical lesions.
In this Box-and-Whisker plot, the median lines represent median values; the upper and lower lines represent the 25th and 75th percentiles, respectively.
The diagnostic power of Septin9 methylation, cytology and HPV16/18 genotyping.
(A) ROC of Septin9 methylation, cytology, HPV16/18 genotyping and Septin9 methylation+HPV16/18 in differentiating cervical cancer patients from non-cancerous patients.
(B) ROC of Septin9 methylation, cytology, HPV16/18 genotyping and Septin9 methylation+HPV16/18 in differentiating ≥ HSIL patients from ≤ LSIL patients.
Comparison of Septin9 methylation with different severity of cervical lesions.
Septin9 DNA methylation as a promising biomarker for cervical cancer

December 2022

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55 Reads

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8 Citations

Aberrant Septin9 methylation in cervical cancer has been rarely studied. We aimed to identify its diagnostic value in cervical cancer using cervical scrapings, and its predictive potential in plasma for pelvic nodal metastasis of cervical cancer. The statuses of methylated Septin9 in fresh cervical lesions and cervical scrapings were first evaluated by using quantitative methylation-specific PCR. Subsequently, the relationship between Septin9 methylation in 113 plasma samples and pelvic nodal metastasis of cervical cancer was evaluated. Methylated Septin9 was detected in all cancerous tissues, but not in cervicitis. The degrees of Septin9 methylation increased with growing severity of cervical lesions in cervical scrapings. The sensitivity of methylated Septin9 was lower than that of cytology, while it yielded a high specificity and area under the curve in detecting high-grade squamous intraepithelial lesion or cervical cancer; and when Septin9 methylation combined with HPV16/18 genotyping, the sensitivity would increase from 70.42% to 82.39%. Plasma-based Septin9 methylation had a high discriminatory power in predicting pelvic nodal metastasis of cervical cancer, with an optimal specificity of 81.48%. In conclusion, we demonstrated methylated Septin9 to be an innovative diagnostic biomarker for cervical cancer and its non-invasive predictive potential in plasma for pelvic nodal metastasis of cervical cancer. Impact statement What is already known on this subject? The occurrence of cervical cancer is related to Septin9 methylation. In fresh specimens and cervical scrapings, we found the degrees of methylated Septin9 increased with growing severity of cervical lesions. Compared with HPV16/18 genotyping and cytological detection, Septin9 methylation had a better specificity and AUC in detecting ≥ HSIL. Furthermore, plasma-based Septin9 methylation also had a high specificity for pelvic lymphatic metastasis prediction. What the results of this study add? Methylation analysis of Septin9 indicated a similar sensitivity, specificity and AUC in detecting ≥ HSIL, relative to HPV16/18 genotyping. Compared with cytological method, Septin9 methylation also yielded a higher specificity and AUC in detecting ≥ HSIL. And we also found plasma-based Septin9 methylation had a high discriminatory power in predicting pelvic nodal metastasis of cervical cancer, with an optimal specificity of 81.48%; additionally an increasing sensitivity from 50% to nearly 80% was found when combined with SCCAg. What the implications are of these findings for clinical practice and/or further research? This study aimed to evaluate the relationship between Septin9 methylation and cervical cancer, and to explore the value of methylated Septin9 in the detection of cervical (pre)cancerous lesions. Moreover, we would explore plasma-based ctDNA biomarkers for pelvic lymphatic metastasis prediction of cervical cancer, to improve non-invasive predictive accuracy of pelvic nodal metastasis and reduce the complications caused by pelvic lymphadenectomy.


Figure 1. NODAL and ALK7 expression levels are decreased in EC cell lines. (A) Bioinformatics analysis was performed to predict the expression of NODAL and ALK7 in EC cells. ALK7 and NODAL (B) mRNA and (C) protein expression levels in EC cells. * P<0.05, ** P<0.01 and *** P<0.001 vs. ESC. NODAL, nodal growth differentiation factor; ALK7, activin A receptor type 1C; EC, endometrial cancer.
Figure 2. NODAL overexpression inhibits EC cell proliferation, invasion and migration. NODAL (A) mRNA and (B) protein expression levels following NODAL overexpression in EC cells. (C) Cell proliferation was assessed by performing a Cell Counting Kit-8 assay. (D) Representative images of the wound healing assay (magnification, x100). (E) Quantification of cell migration. (F) Representative images of the Transwell assay (magnification, x100). (G) Quantification of cell invasion. (H) MMP2, MMP7 and MMP9 protein expression levels were assessed via western blotting. *** P<0.001 vs. Ov-NC. NODAL, nodal growth differentiation factor; EC, endometrial cancer; MMP, matrix metallopeptidase; Ov, overexpression; NC, negative control; OD, optical density.
Figure 3. NODAL overexpression promotes endometrial cancer cell apoptosis. (A) Cell apoptosis was assessed by performing a TUNEL assay (magnification, x200). (B) Expression levels of apoptosis-related proteins were assessed via western blotting. *** P<0.001 vs. Ov-NC. NODAL, nodal growth differentiation factor; Ov, overexpression; NC, negative control.
Figure 4. ALK7 inhibition reverses NODAL overexpression-mediated effects on endometrial cancer cell proliferation. (A and B) ALK7 protein expression levels in transfected cells were measured via western blotting. *** P<0.001 vs. Ov-NC; ## P<0.01, ### P<0.001 vs. shRNA-NC. (C) ALK7 expression levels in co-transfected cells were measured via reverse transcription-quantitative PCR. ** P<0.01 and *** P<0.001 vs. Ov-NODAL-NC. (D) Cell proliferation was assessed by performing Cell Counting Kit-8 assays. (E) Representative images of the wound healing assay (magnification, x100). (F) Quantification of cell migration. (G) Representative images of the Transwell assay (magnification, x100). (H) Quantification of cell invasion. (I) MMP2, MMP7 and MMP9 protein expression levels were measured via western blotting. *** P<0.001 vs. Ov-NC; ## P<0.01 and ### P<0.001 vs. Ov-NODAL-NC; ∆∆ P<0.01 and ∆∆∆ P<0.001 vs. Ov-NODAL. ALK7, activin A receptor type 1C; NODAL, nodal growth differentiation factor; Ov, overexpression; NC, negative control; MMP, matrix metallopeptidase; shRNA, short hairpin RNA; OD, optical density.
Figure 5. ALK7 inhibition reverses NODAL overexpression-mediated promotion of endometrial cancer cell apoptosis. (A) Cell apoptosis was assessed by performing TUNEL assays (magnification, x200). (B) Expression levels of apoptosis-related proteins were measured via western blotting. *** P<0.001 vs Ov-NC; ### P<0.001 vs. Ov-NODAL-NC; ∆∆ P<0.01 and ∆∆∆ P<0.001 vs. Ov-NODAL. ALK7, activin A receptor type 1C; NODAL, nodal growth differentiation factor; Ov, overexpression; NC, negative control.
Biological effects of NODAL on endometrial cancer cells and its underlying mechanisms

February 2021

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45 Reads

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3 Citations

Experimental and Therapeutic Medicine

Activin A receptor type 1C (ALK7) and its ligand nodal growth differentiation factor (NODAL) serve numerous roles in cancer cells, including regulating cancer invasion, migration and apoptosis. NODAL promotes breast cancer cell apoptosis by activating ALK7; however, ALK7 and NODAL expression in endometrial cancer (EC), as well as their effects and underlying mechanisms in EC cells, are not completely understood. The present study aimed to characterize the expression of NODAL and ALK7 in EC, as well as the underlying mechanisms. The expression levels of ALK7 and NODAL were detected via reverse transcription-quantitative PCR and western blotting. Cell transfection was performed to overexpress NODAL or interfere ALK7. Cell proliferation, invasion and migration were detected via Cell Counting Kit-8, Transwell and wound healing assays, respectively. Flow cytometry was performed to detect cell apoptosis and western blotting was conducted to detect the expression levels of apoptosis-related proteins. NODAL and ALK7 expression levels were significantly decreased in EC cell lines compared with normal endometrial cells. NODAL overexpression inhibited EC cell proliferation, invasion and migration, and promoted EC cell apoptosis compared with the overexpression-negative control (Ov-NC) group. Moreover, NODAL overexpression significantly increased ALK7 expression levels in EC cells compared with the Ov-NC group. ALK7 reversed NODAL overexpression-mediated inhibition of EC cell proliferation, invasion and migration, and promotion of EC cell apoptosis. The present study indicated that NODAL inhibited EC cell proliferation, invasion and migration, and promoted EC cell apoptosis by activating ALK7.


Citations (20)


... ADAR1 has been shown to play dual roles in cancer, where its RNA editing promotes tumor growth and immune evasion [174,175]. A high-throughput virtual screening study identified lithospermic acid and regal side B as potential small-molecule inhibitors of ADAR1p150 that target the Zα domain and can inhibit protein translation and arrest tumor cell proliferation and thus exert antitumor effects [177]. Recent research shows that rebecsinib is a potential clinical ADAR1p150 antagonist [178]. ...

Reference:

A Wonderful Journey: The Diverse Roles of Adenosine Deaminase Action on RNA 1 (ADAR1) in Central Nervous System Diseases
High-throughput virtual screening to identify potential small molecule inhibitors of the Zα domain of the adenosine deaminases acting on RNA 1(ADAR1)

European Journal of Pharmaceutical Sciences

... (C) 1. Invasive cancer cells acquire various mechanisms for translocation and penetration through the stromal matrix and myosin light chains MYL5, MLC-2, MLC20, and MYL9 help cancer cells gain invasive properties through mechanotransduction and control of genetic factors [21,24,25,[27][28][29][30]. ...

MYL9 promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis

... Based on the median risk score, the patients were categorized into high-risk and low-risk groups, and the relationships between HRRS and gene expression levels, survival time and survival status were analyzed. Similarly, two previously reported seven-gene signature (ACSL1, ALDOA, FOXK2, GPI, MDH1B, MDH2 and MTHFD1) and the six-gene signature (HSPA5, ANGPTL4, PFKM, GOT1, IER3 and PFKFB4) were calculated according to their mentioned corresponding coefficients [21,22] and the gene expression levels in cisplatin-treated patients (TCGA cohort). Patients were stratified into high-risk and low-risk groups according to the median level of the six-gene signature or seven-gene signature for following survival analysis. ...

The construction and analysis of tricarboxylic acid cycle related prognostic model for cervical cancer

... However, not all of these genes are suitable for cervical cancer diagnosis. He et al. (2023) demonstrated a strong association between SEPT9 methylation and PAX1 methylation with cervical cancer. The methylation levels of SEPT9 and PAX1 were found to have predictive value for the severity of cervical lesions in women positive for high-risk human papillomavirus (HR-HPV), with SEPT9 methylation emerging as a more effective biomarker. ...

PAX1 and SEPT9 methylation analyses in cervical exfoliated cells are highly efficient for detecting cervical (pre)cancer in hrHPV-positive women

... Liang et al.'s 130 model, PulmoSeek, which incorporates 100 methylation features, demonstrated high sensitivity in detecting early-stage LC, outperforming existing diagnostic models and positron emission tomography-computed tomography in precision. In addition, Bu et al. 131 evaluated the application of Septin9 gene methylation in the diagnosis of cervical cancer and prediction of pelvic lymph node metastasis, demonstrating its potential as a novel biomarker. Lung EpiCheck, a novel blood test based on six methylation markers, has shown high detection rates for early non-small cell lung cancer (NSCLC) and SCLC in high-risk populations. ...

Septin9 DNA methylation as a promising biomarker for cervical cancer

... It plays a role in physiological processes such as embryonic development, tissue remodeling and stem cell maintenance [60]. However, NODAL are highly expressed in several malignant tumors, such as melanoma, breast cancer, gastric cancer, HCC, etc., and negatively correlates with the degree of differentiation, clinical stage, metastasis, and prognosis of the tumors [61][62][63]. NODAL is an important component of the TGF-β signaling pathway which enhances the proliferation, migration, and invasion of HCC cells by promoting the phosphorylation of Smad3 and the expression of Snail, drug resistance, and epithelial-mesenchymal transition (EMT) [64]. In addition, NODAL has been shown to modulate tumor cell plasticity by promoting the formation of angiogenic mimetic structures [7]. ...

Biological effects of NODAL on endometrial cancer cells and its underlying mechanisms

Experimental and Therapeutic Medicine

... Furthermore, substantial geographical variation exists in the prevalence and distribution of HPV infections [6]. The disease risk associated with HPV infection also varies significantly based on the genotype, persistence, and duration of the infection [7][8][9]. Consequently, identifying the genotype and monitoring the persistence of HPV infection are of considerable clinical importance. ...

Incidence, persistence and clearance of cervical human papillomavirus among women in Guangdong, China 2007–2018: A retrospective cohort study

Journal of Infection and Public Health

... For spontaneous abortion (n = 7), despite most studies having indicated a harmful direction of effect, the evidence lacked consistency, with five studies yielding no statistically significant association for spontaneous abortion 30,[44][45][46][47] , and one study exhibited an inverse U-shaped association 48 . ...

Associations of ambient temperature exposure during pregnancy with the risk of miscarriage and the modification effects of greenness in Guangdong, China
  • Citing Article
  • November 2019

The Science of The Total Environment

... HPV genotypes HPV16 and 18 were identified in 86.2% (1,645/1,908) of the patients, which is consistent with these being the most common oncogenic HPV genotypes worldwide (19), followed by HPV genotypes HPV58, 33, and 52. While, consistent with literature reports on geographical variations in HPV distribution, our study results differ from the predominant HPV genotype distributions reported in other regions of China (12,15,(20)(21)(22)(23)(24). A potential reason for this discrepancy may be our inclusion criteria. ...

Cervical human papillomavirus among women in Guangdong, China 2008-2017: Implication for screening and vaccination

Journal of Medical Virology

... The methylation status of cytosine (C) in CpG dinucleotides in the promoter regions of human genes such as FAM19A4, EPB41L3 or PAX1 has been found to be significantly different in cancerous cervical exfoliated cells, compared to normal cervical exfoliated cells [8][9][10] . However, previous studies usually had small sample sizes and mostly focused on a single gene, resulting in low sensitivity and specificity for diagnosing CC. ...

The clinical significance of FAM19A4 methylation in high-risk HPV-positive cervical samples for the detection of cervical (pre)cancer in Chinese women

BMC Cancer