Xinye Wang’s research while affiliated with Qinghai Normal University and other places

Carbon-sequestering microorganisms play an important role in the carbon cycle of wetland ecosystems. However, the response mechanism of carbon-sequestering microbial communities to wetland type changes and their relationship with soil carbon remain unclear. To explore these differences and identify the main influencing factors, this study selected marsh wetlands, river wetlands and lakeside wetlands around Qinghai Lake as research subjects. High-throughput sequencing was employed to analyze the functional gene cbbM of carbon-sequestering microorganisms. The results revealed that the alpha diversity of cbbM carbon-sequestering microorganisms mirrored the trend in total carbon content, with the highest diversity observed in marsh wetlands and the lowest in lakeside wetlands. The dominant bacterial phylum was Proteobacteria, with prevalent genera including Thiothrix, Acidithiobacillus, and Thiodictyon. Acidithiobacillus served as a biomarker in lakeside wetlands, while two other genera were indicative of marsh wetlands. The hierarchical partitioning analysis indicated that the diversity of cbbM carbon-fixing microorganisms was primarily influenced by the total nitrogen content, while the community structure was significantly affected by the soil total carbon content. Moreover, an increased soil temperature and humidity were found to favor the carbon fixation processes of Thiomicrospira, Thiomonas, Polaromonas, and Acidithiobacillus. In summary, changes in wetland types seriously affected the characteristics of cbbM carbon sequestration in microbial communities, and a warm and humid climate may be conducive to wetland carbon sequestration.

Global climate change has altered the frequency of soil freeze–thaw cycles, but the response of soil microorganisms to different elevation gradients during the early freeze–thaw period remains unclear. So far, the influence of the altitudinal gradient on the microbial community and metabolic characteristics in the early freeze–thaw period of the Qinghai Lake Basin remains unclear. To this end, we collected soil at different elevations in the early freeze–thaw period of the Qinghai Lake Basin and investigated the influence of the elevation gradient on soil microbial community characteristics and soil metabolic processes as well as the corresponding environmental driving mechanism by high-throughput sequencing and LC–MS (Liquid Chromatograph-Mass Spectrometer) nontargeted metabolite determination. The results showed that Proteobacteria were the dominant microflora in the Qinghai Lake Basin. The dominant phyla associated with carbon and nitrogen are Proteobacteria and Firmicutes, both of which are significantly affected by elevation. The soil physicochemical factors jointly affected the soil microbial communities and metabolism. Total phosphorus nitrate nitrogen and pH were the main driving factors of the microbial community, and metabolites were sensitive to changes in chemical factors. In short, the microbial community structure and function, soil physicochemical factors and soil metabolic processes were significantly affected by the altitudinal gradient in the early freeze–thaw period, while the microbial community diversity showed no significant response to the altitudinal gradient. Additionally, a high potassium content in the soil may promote the growth and reproduction of bacteria associated with carbon and nitrogen cycling, as well as the production of metabolites.

The temperature, moisture, and salt content of soil in alpine regions are sensitive to changes in climatic factors and are important indicators of ecosystem functions. In this study, we collected soil moisture, temperature and electrical conductivity data at different depths at a sampling site on Bird Island in Qinghai Lake during winter using a continuous soil temperature, moisture and salt content monitoring system and analyzed their variations and influential factors. The variation in soil moisture showed an obvious ‘V-shaped’ pattern from 00:00 to 23:00 and an upward trend with soil layer depth. From 00:00 to 23:00, the overall soil temperature data fitted a ‘unimodal’ curve and showed a clear and continuous upward trend with soil layer depth at a rate of 0.684 (p < 0.001). Soil electrical conductivity data also exhibited a distinct ‘V-shaped’ pattern from 00:00 to 23:00 and a continuous increase with increasing soil depth. The correlation between soil temperature, moisture, and conductivity and the spatial distribution of five climate factors indicated that climate factors accounted for 53.6% of the changes in soil temperature, moisture, and salinity. Climate factors showed a significant positive correlation with soil temperature, moisture, and conductivity (p < 0.001), and air temperature was the most important factor influencing soil temperature and soil moisture changes, whereas wind direction was the most important factor influencing soil conductivity. (Wind direction and wind speed affect soil evapotranspiration, and then affect soil moisture and solute transport process). The results of this preliminary study reveal the characteristics associated with soil temperature, moisture, and salinity changes in winter within the wetlands of Bird Island on Qinghai Lake in the context of climate change, and they can be used as valuable reference data in further studies investigating associated changes in ecosystem functions.