Xiaowei Fan’s research while affiliated with Beijing Tiantan Hospital and other places

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Publications (1)

Flow of the participants during the study. ¶ indicates physical discomfort during the scan. ⧫ indicates poor data quality.
The GABA level of the right thalamus exhibited a significant decrease in the is-MCI patients. (A) and (B) demonstrates the voxel placement in the left and right thalamus. (C) Multiple comparisons analysis of least significant difference showed that GABA/water ratio in IS-MCI group was lower than that in HC, IS and MCI groups (* show p < 0.05, versus HC; △show p < 0.05, versus IS; + show p < 0.05, versus MCI). (D) Post hoc analysis showed that GABA/Cr ratio in IS-MCI group was lower than that in HC, IS and MCI groups (* show p < 0.05, versus HC; △show p < 0.05, versus IS-NC; + show p < 0.05, versus MCI). (E, F) Glx/water and Glx/Cr ratios were not significantly different among groups.
Correlation analysis revealed glx levels of the right thalamus were linked with cognition. (A, B) Lower Glx/water and Glx/Cr ratios in the right thalamus were associated with lower score of the MoCA scale after adjusting for confounding factors such as age, gender, education, sleep quality (PSQI), depression (PHQ-9) and anxiety (GAD-7), but these associations were not signification. (C, D) Working memory (DST-F) was weak associated with Glx levels in the right thalamus after adjusting for above confounding factors. (E, F) There is no significant association between information processing speed (DSST) and Glx levels in the right thalamus after adjusting for above confounding factors.
Proton magnetic resonance spectroscopy in hippocampi of rats revealed a change in relative metabolite ratios. (A, B) Representative pictures of ¹H-MRS in the left and right hippocampus. The morphological T2-weight MR images illustrated the position of the CSI grid covering both lateral hippocampus (green grid and blue boxes on MRI). (C) There are displayed representative MRS-spectra of the selected voxel in hippocampus. (D-F) Concentration maps of NAA, Cho and Glx from a representative rat in Con, SD, Aβ and SA groups respectively. (G) The graph of NAA/Cr ratio (% of con) demonstrated a severe reduction in right side of hippocampus in SA group, whereas, there was no statistically difference in the left side. (H) The Cho/Cr ratio (% of Con) decreased in bilateral hippocampus of SA group. (I) The Glx/Cr ratio (% of Con) showed an opposite change tendency in SD group; revealed a difference between bilateral hippocampus in SA group. (J, K) The correlation analysis between metabolite concentration in left and right hippocampus and discrimination ratio (DI) of NOR. * versus Con, **p < 0.01, ***p < 0.001; △ versus SD, △△p < 0.01, △△△p < 0.001; ⁺ versus Aβ, ⁺⁺⁺p < 0.001; Paired samples t test between bilateral hippocampal betweenness centrality in four groups. ##p < 0.01.
Sleep deprivation aggravates aβ-induced synaptic impairment and activation of astrocyte. (A) The representative electron microscopy images of bilateral hippocampus synapses in four groups; Scale bar = 500 nm. (B) Quantitative analysis of synaptic gap width (% of Con) showed that Aβ induced the increase of synaptic gap, especially on the right side. (C) Quantitative analysis of PSD thickness (% of Con) showed that sleep deprivation could induce a decrease in PSD thickness, which was more significant on the right side of SA group than on the left. (D) Quantitative analysis of vesicle spacing (% of Con) in the active region showed a significant decrease in the right side of SD group. (E) Quantitative analysis of the number of vesicles in the active zone (% of Con) showed that sleep deprivation and Aβ induced a decrease in the number of vesicles in the active zone. (F) Minimum vesicle adjacent distance (% of Con) quantitative analysis showed that sleep deprivation could induce the increase of minimum adjacent distance under the condition of Aβ. (G) Quantitative analysis of vesicle proportion of active region (% of Con) showed that Aβ could induce an increase in the proportion, especially in the right side. (H) Immunofluorescence staining of GFAP suggested that sleep deprivation aggravated astrocytic reactivation induced by Aβ; Scale bar = 150 μm. (I, J) Sholl analysis revealed an increased number of intersections of bilateral hippocampus astrocytes. * versus Con, **p < 0.01, ***p < 0.001; △ versus SD, △△p < 0.01, △△△p < 0.001; ⁺ versus Aβ, ⁺⁺⁺p < 0.001; Independent sample t test between bilateral hippocampal betweenness centrality in four groups. ##p < 0.01.

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Sleep disturbance impaired memory consolidation via lateralized disruption of metabolite in the thalamus and hippocampus: A cross-sectional proton magnetic resonance spectroscopy study
  • Article
  • Publisher preview available

November 2024

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22 Reads

Xiaowei Fan

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Xin Mao

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Ping Yu

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[...]

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Chunxue Wang

Background Memory consolidation in sleep-dependent individuals involves the circuitry connections of cortex, thalamus and hippocampus, regulating via neural metabolites. However, the disruption of metabolic pattern in thalamus and hippocampus remains unclear. Objective We aim to explore the disruptive effects of insomnia on the metabolites during memory consolidation, particularly the underlying neurometabolic mechanisms in comorbidity of failed memory consolidation. Methods This study integrates clinical research with animal experiment. In clinical research, 49 participants were divided into four groups: healthy controls (HC, n = 11), insomnia with normal cognition (IS, n = 14), mild cognitive impairment without insomnia (MCI, n = 10), and insomnia with mild cognitive impairment (IS-MCI, n = 14). Magnetic resonance spectroscopy (MRS) was used to evaluate the neural γ-aminobutyric acid (GABA) and glutamate–glutamine (Glx) in bilateral thalamus. In experimental studies, the rat model of sleep deprivation combined with amyloid-β (Aβ) injection was established, after behavior testing, the levels of Glx, choline (Cho) and N-acetyl aspartate (NAA) in the bilateral hippocampus were evaluated with MRS. Results The patients in the IS-MCI group exhibited significantly lower GABA level than IS, MCI and HC groups. Results from rat studies showed that sleep deprivation exacerbated asymmetric alterations in Aβ-induced bilateral hippocampal metabolite abnormalities, which correlated with cognition. These neuro-metabolite disruption accompanied with synaptic loss and activation of astrocytes. Conclusions The lateralized decrease in GABA levels of thalamus and NAA, Cho, and Glx levels of hippocampus under conditions of sleep disturbance with cognitive decline may provide evidence for the neural metabolic mechanisms underlying the disruption of memory consolidation.

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