Xiaoping Chen’s research while affiliated with Yangzhou University and other places

What is this page?


This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.

Publications (5)


Fig. 1. Study flow diagram. VT, tidal volume.
Table 6 .
Table 8 .
Drive Pressure-Guided Individualized Positive End-Expiratory Pressure in Traumatic Brain Injury Surgery: A Randomized Controlled Trial
  • Article
  • Full-text available

December 2024

·

8 Reads

Xiaoping Chen

·

Zi Wang

·

Yali Ge

·

[...]

·

Liuqing Yang

AIM: Intraoperative lung-protective ventilation strategies (LPVS) have been shown to improve lung oxygenation and prevent postoperative pulmonary problems in surgical patients. However, the application of positive end-expiratory pressure (PEEP)-based LPVS in emergency traumatic brain injury (TBI) has not been thoroughly explored. The purpose of this study is to evaluate the effects of drive pressure-guided individualized PEEP on perioperative pulmonary oxygenation, postoperative pulmonary complications, and recovery from neurological injury in patients with TBI. METHODS: A total of 111 TBI patients who met the inclusion criteria at Northern Jiangsu People's Hospital were randomized into three groups: group A (0 PEEP, 50% inhaled oxygen concentration, and 6 mL/kg tidal volume), group B (5 cmH2O PEEP, 50% inhaled oxygen concentration, and 6 mL/kg tidal volume), and group C (individualized PEEP guided by driving pressure, 50% inhaled oxygen concentration, and 6 mL/kg tidal volume). The primary endpoints were lung ultrasound score (LUS), optic nerve sheath diameter (ONSD), and serum levels of neuron-specific enolase (NSE) and High mobility group box 1 protein (HMGB1). Secondary endpoints included intraoperative hemodynamic and respiratory mechanics parameters, postoperative pulmonary complications, and clinical lung infection scores. RESULTS: Eighty-nine patients completed the final analysis. LUS was significantly lower in group C compared to group A at T4 (least square mean [95% confidence interval (CI)]: 2.50 [1.35, 3.65] vs. 5.25 [4.10, 6.40], p < 0.05). Although ONSD increased gradually in group C, it did not differ substantially from group A postoperatively (least square mean [95% CI]: 5.09 [4.90, 5.27] vs 5.16 [4.97, 5.34] mm, p > 0.05). Serum NSE levels in group C were significantly lower on postoperative days 1 (4.40 [3.89, 4.41] vs. 10.95 [10.44, 11.46], p < 0.05) and 3 (2.79 [2.28, 3.30] vs. 10.95 [10.44, 11.46], p < 0.05). Additionally, serum HMGB1 levels in group C were significantly reduced on postoperative days 1 (229 [200, 258] vs. 662 [633, 691], p < 0.05) and 3 (166 [137, 195] vs. 662 [633, 691], p < 0.05). CONCLUSIONS: Individualized PEEP guided by driving pressure can improve perioperative pulmonary oxygenation and reduce the incidence of postoperative pulmonary complications. Furthermore, this strategy did not significantly elevate intraoperative intracranial pressure (ICP) and promoted recovery from postoperative neurological injury, likely by reducing the inflammatory response. CLINICAL TRIAL REGISTRATION: https://www.chictr.org.cn/ (clinical trial no. ChiCTR2200066795).

Download

Decoding neuronal genes in stroke-induced pain: insights from single-nucleus sequencing in mice

November 2024

·

3 Reads

BMC Neurology

Background The role of neurons in central post-stroke pain (CPSP) following thalamic hemorrhage remains unclear. This study aimed to identify key genes associated with post-thalamic hemorrhage pain and to explore their functions in neurons. Single-nucleus RNA sequencing (snRNA-seq) data from a mouse model was used for this analysis. Methods First, snRNA-seq data were analyzed to identify cell types associated with CPSP induced by thalamic hemorrhage. Differentially expressed genes (DEGs) in neurons were then screened between control and model groups, followed by the construction of a protein-protein interaction (PPI) network for the DEGs. CytoNCA was used to assess node connectivity in the PPI network, and the top 5 key genes were identified. Subsequently, transcription factor (TF)-mRNA and miRNA-mRNA networks were constructed, and small-molecule drugs potentially targeting these key genes were predicted. Finally, the expression differences of key genes in neurons were compared between the model and control groups. Results A total of 13 cell clusters were identified, categorized into 8 cell types: T cells, endothelial cells, monocytes, neural progenitor cells (NPCs), microglia, astrocytes, neurons, and oligodendrocytes. A total of 228 DEGs were detected in neurons when comparing the model group with the control group. The PPI network of the DEGs consisted of 126 nodes and 209 edges, identifying the top 5 key genes: Dlgap1, Cacna1c, Gria2, Hsp90ab1, and Gapdh. The miRNA-mRNA network included 68 miRNA-mRNA pairs, 62 miRNAs, and 5 mRNAs, while the TF-mRNA network consisted of 66 TF-mRNA pairs, 56 TFs, and 5 mRNAs. Drug prediction identified 110 small-molecule drugs (e.g., purpurogallin, nifedipine, and novobiocin) potentially targeting these key genes. Additionally, Cacna1c were significantly upregulated in model mice. Conclusion This study identified the role of key genes in thalamic hemorrhage-induced CPSP through snRNA-seq, providing a scientific basis for further exploration of the molecular mechanisms underlying CPSP.


Figure 2. Effect of NLRP3-siRNA on thalamic pain. Baseline behavior tests were performed one day prior to siRNA/scramble injection into the VPL and VPM nuclei. Three days after the administration of siRNA or scramble, Coll IV or saline was microinjected into the VPL and VPM nuclei. Administration of siRNA significantly attenuated the increase in paw withdrawal frequency in response to (A) 0.07 g and (B) 0.4 g von Frey filaments and the decrease in paw withdrawal latency to (C) thermal and (D) cold stimuli at day 1, 3 and 5 following Coll IV microinjection on the contralateral side. (E-G) Administration of siRNA or scramble did not cause behavioral alterations on the ipsilateral side. n=8 mice/group. * P<0.05 vs. saline + scramble at the corresponding time points; # P<0.05 vs. Coll IV + scramble at the corresponding time points. NLRP3, NLR family pyrin domain containing 3; siRNA, small interfering RNA; VPL, ventral posterior lateral; VPM, ventral posterior medial; Coll IV, collagenase IV.
Figure 3. Effect of NLRP3 siRNA on NLRP3 inflammasome activity and the downstream factors IL-18, IL-1β, GFAP and p-ERK1/2. (A) Transfection with NLRP3 siRNA significantly decreased the protein expression levels of caspase-1, ASC and NLRP3. (B) NLRP3 siRNA also significantly reduced IL-18 and IL-1β protein expression levels. (C) After microinjection of NLRP3 siRNA, the protein expression levels of p-ERK1/2 and GFAP significantly decreased. n=3 mice/group. * P<0.05 vs. saline + scramble; # P<0.05 vs. Coll IV + scramble. NLRP3, NLR family pyrin domain containing 3; siRNA, small interfering RNA; GFAP, glial fibrillary acidic protein; p, phosphorylated; ASC, apoptosis-associated speck-like protein containing a CARD.
Figure 4. Effect of miR-223 agomir on thalamic pain. Baseline behavior tests were performed 1 day prior to miR-223 agomir or agomir-scramble microinjection into the VPL and VPM nuclei. Three days after administration of miR-223 agomir/agomir scramble, Coll IV or saline was microinjected into the VPL and VPM nuclei. Administration of miR-223 agomir significantly attenuated the increase in paw withdrawal frequency in response to (A) 0.07 g and (B) 0.4 g von Frey filaments and the significant decrease in paw withdrawal latency to (C) thermal and (D) cold stimuli on day 1, 3 and 5 following Coll IV microinjection on the contralateral side. (E-G) Administration of miR-223 agomir or agomir scramble did not affect behavioral changes on the ipsilateral side. n=8 mice/group. * P<0.05 vs. saline + agomir-scramble at the corresponding time points; # P<0.05 vs. Coll IV + agomir-scramble at the corresponding time points. miR, microRNA; VPL, ventral posterior lateral; VPM, ventral posterior medial; Coll IV, collagenase IV.
Figure 7. NLRP3 inflammasome inhibitor rescues miR-223 antagomir-induced thalamic pain in naïve mice. Tail vein injection of CY 09 (10 mg/kg) significantly attenuated the increase in paw withdrawal frequency in response to (A) 0.07 g and (B) 0.4 g von Frey filaments and the decrease in paw withdrawal latency to (C) thermal and (D) cold stimuli on day 3, 5 and 7 on the contralateral side. (E-G) Injection of CY 09 or vehicle did not cause behavioral changes on the ipsilateral side. n=8 mice/group. * P<0.05 vs. naïve group at the corresponding time points; # P<0.05 vs. naïve + miR-223 antagomir + vehicle group at the corresponding time points. NLRP3, NLR family pyrin domain containing 3; miR, microRNA.
Figure 8. CY 09 affects IL-18, IL-1β, p-ERK1/2 and GFAP protein expression levels. (A) CY 09 injection caused no alterations in miR-223 expression levels. CY 09 injection (B) significantly reduced IL-18 and IL-1β protein expression levels in the naïve + miR-223 antagomir cohort and (C) significantly lowered the protein expression levels of GFAP and p-ERK1/2. n=3 mice/cohort. * P<0.05 vs. naïve group; # P<0.05 vs. miR-223 antagomir + vehicle. GFAP, glial fibrillary acidic protein; p, phosphorylated; miR, microRNA.
miR‑223 ameliorates thalamus hemorrhage‑induced central poststroke pain via targeting NLRP3 in a mouse model

March 2022

·

70 Reads

·

13 Citations

Experimental and Therapeutic Medicine

Central poststroke pain (CPSP) is a central neuropathic pain syndrome that occurs following a stroke and mainly manifests as pain and paresthesia in the body region corresponding to the brain injury area. At present, due to the lack of clinical attention given to CPSP, patients suffer from long-term pain that seriously affects their quality of life. Current literature indicates that microRNA (miR)-223 can impede inflammation and prevent collateral damage. The NLR family pyrin domain containing 3 (NLRP3) inflammasome induces IL-18 and IL-1β secretion and maturation and participates in the inflammatory response. Previous evidence has confirmed that miR-223 can negatively regulate NLRP3 in the development of inflammatory responses. However, whether the miR-223 targeting of NLRP3 is involved in CPSP remains unclear. In the present study, the expression of miR-223 was detected by reverse transcription-quantitative PCR analysis. The expression levels of NLRP3, caspase-1, ASC, IL-18, IL-1β, ERK1/2, p-ERK1/2 and GFAP were detected by western blot analysis. The results demonstrated that thalamic hemorrhagic stroke triggered by microinjection of collagenase Ⅳ (Coll IV) into the ventral posterior lateral (VPL) nucleus results in pain hypersensitivity. miR-223 expression level were significantly reduced in the CPSP model. The expression levels of NLRP3, caspase-1, ASC, IL-18 and IL-1β were significantly increased in the CPSP model. The expression level of GFAP was detected to determine astrocyte activation. The results demonstrated that astrocyte activation induced by Coll IV produced a CPSP model. The p-ERK1/2 expression level was demonstrated to be significantly increased in the CPSP model. The introduction of an miR-223 agomir significantly attenuated thalamic pain and significantly decreased the levels of NLRP3, caspase-1, ASC and proinflammatory cytokines (IL-18 and IL-1β). Furthermore, introducing a miR-223 antagomir into the VPL nucleus of naïve mice mimicked thalamic pain and significantly increased the levels of NLRP3, caspase-1, ASC and proinflammatory cytokine levels (IL-18 and IL-1β). These results indicated that miR-223 inhibited NLRP3 inflammasome activity (caspase-1, NLRP3 and ASC), which ameliorated thalamus hemorrhage-induced CPSP in mice via NLRP3 downregulation. In conclusion, these results may determine the mechanisms underlying CPSP and facilitate development of targeted therapy for CPSP.



Effects of transcutaneous electrical acupoint stimulation on quality of recovery during early period after laparoscopic cholecystectomy

March 2018

·

3 Reads

·

11 Citations

Zhongguo zhen jiu = Chinese acupuncture & moxibustion

Objective: To evaluate the effect of transcutaneous electrical acupoint stimulation (TEAS) on the quality of recovery during the early period after laparoscopic cholecystectomy and the dosage of anesthetic and analgesic. Methods: One hundred patients who received laparoscopic cholecystectomy with gradeⅠand Ⅱ of American Society of Anesthesiologists (ASA) criteria were randomly assigned into an observation group and a control group according to random number table, 50 cases in each group. The patients in the two groups were treated with conventional endotracheal intubation anesthesia, anesthesia induction and maintenance. The patients in the observation group were treated with TEAS (2 Hz/100 Hz, 8 to 12 mA) at bilateral Hegu (LI 4) and Neiguan (PC 6), as well as Zusanli (ST 36) and the non-acupoint 2 cun outboard from Zusanli (ST 36) from 30 min before anesthesia induction to the end of operation. The patients in the control group were applied by stimulation electrode in the corresponding points without electrical stimulation. The dosage of intraoperative remifentanil and the analgesic dosage of dezocine for postoperation were recorded. The recovery time, extubation time, the changes of heart rate (HR) and mean arterial pressure (MAP) during extubation were recorded. The quality of recovery was assessed by the quality of recovery-40 questionnaire (QoR-40) 1 day before surgery (T0),and 4 h (T1), 8 h (T2), 24 h (T3), 48 h (T4) after surgery. The patient's cognitive function was assessed by mini-mental state examination (MMSE) scale at the 5 time points. The incidences of postoperative nausea and vomiting were recorded at T1 through T4. Results: The dosages of intraoperative remifentanil and dezocine in the observation group were less than those in the control group; the recovery time and extubation time were shorter than those in the control group; the HR of extubation was lower than that in the control group (all P<0.05). There was no statistic difference about MAP between the two groups (P>0.05). Compared with T0, the total scores of QoR-40 decreased in the two groups at T1, T2, T3 (all P<0.05), and the total scores in the observation group were higher than those in the control group (all P<0.05). The emotional state, physical comfort, psychological support, self-care ability, pain scores at T1 in the observation group and at T1, T2, T3 in the control group were lower than those at T0 (all P<0.05). The emotional state, physical comfort, psychological support, self-care ability, pain scores in the observation group were higher than those in the control group at T1, T2, T3 (all P<0.05). Compared with T0, the MMSE scores in the two groups decreased at T1 and T2 (all P<0.05). At T1, T2, T3, the MMSE scores in the observation group were higher than those in the control group (all P<0.05). At T1 and T2, the incidence rates of nausea and vomiting were 22.0% (11/50), 12.0% (6/50) respectively in the observation group, which were lower than 32.0% (16/50) and 24.0% (12/50) in the control group (both P<0.05). At T3 and T4, the incidence rates of nausea and vomiting were 6.0% (3/50), 2.0% (1/50) respectively in the observation group, which were not significantly different from 8.0% (4/50) and 4.0% (2/50) in the control group (both P>0.05). Conclusion: TEAS can improve the quality of recovery during the early period after laparoscopic cholecystectomy and reduce the dosage of anesthetic and analgesic.

Citations (3)


... The SAH model was established using a modified single-clamp puncture method (40). Mice were initially anesthetized with 5% isoflurane and then maintained under anesthesia with 2% isoflurane (42). Mice were positioned supine on the operating table, and the depth of anesthesia was confirmed, ensuring no signs of discomfort at the incision site. ...

Reference:

NGR1 reduces neuronal apoptosis through regulation of ITGA11 following subarachnoid hemorrhage
miR‑223 ameliorates thalamus hemorrhage‑induced central poststroke pain via targeting NLRP3 in a mouse model

Experimental and Therapeutic Medicine

... The VCV can maintain a certain amount of ventilation and is easy to operate. However, if the dynamic lung compliance (Cdyn) decreases or the airway resistance (Raw) increases, it will induce high airway pressure and aggravate the degree of lung injury [5]. As one of the auxiliary functions of Zeus anesthesia machine, auto flow combines the advantages of VCV and PCV, and is the expansion and supplement of various constant volume ventilation modes [6]. ...

Effect of perioperative mechanical ventilation strategies on postoperative pulmonary complications in patients undergoing thoracic surgery:a Meta-analysis
  • Citing Article
  • March 2021

Asian Journal of Surgery

... 48 (2021) demonstrated the efficacy of TEAS in alleviating catheter-related bladder discomfort 49 and improving overall postoperative comfort in patients undergoing transurethral resection 50 of the prostate [5]. Moreover, Mi et al. (2018) found that TEAS improved the quality of 51 recovery during the early period after laparoscopic cholecystectomy [6]. These studies col-52 lectively underscore the broad applicability and safety of TEAS in various surgical contexts. ...

Effects of transcutaneous electrical acupoint stimulation on quality of recovery during early period after laparoscopic cholecystectomy
  • Citing Article
  • March 2018

Zhongguo zhen jiu = Chinese acupuncture & moxibustion