Xiaobo Zhang's research while affiliated with Dalian Medical University and other places

Publications (5)

Article
Objective This present study aims to assess the effect of pirfenidone (PFD) on inhibiting fibroblast proliferation, migration or adhesion in vitro and reducing laminectomy-induced epidural fibrosis in vivo. Methods The effect of PFD on proliferation inhibition was evaluated with flow cytometry, CCK-8, EdU and western-blotting assays. Altered prope...
Article
Post-surgery arthrofibrosis is one of the most restrictive factors in the development of intra-articular surgery and has presented tremendous obstacles for most orthopaedic surgeons. Tanshinone IIA (Tan IIA), a key active ingredient of Den-shen, has been used to treat fibrosis-related diseases, such as pulmonary, hepatic and myocardial fibrosis. In...
Article
Objective: To investigate the effects of everolimus (EVE) on postoperative fibrosis in the knee joint and the potentially relevant signaling pathways. Methods: CCK-8 and flow cytometry assays were used to detect the effect of EVE on human fibroblast viability and apoptosis induction. IF and TEM were used to assess fibroblast autophagy. 3-methyla...
Preprint
Full-text available
Background Excessive fibroblasts proliferation is believed as a major reason in the process of epidural fibrosis, which is known as a troublesome complication of lumbar laminectomy clinically. Mycophenolate mofetil (MMF) is a kind of immunosuppressant, previous studies had shown that it has the function of preventing fibrosis, but the role and mech...
Article
Full-text available
Background: Failed back surgery syndrome (FBSS) is a common complication after the laminectomy. Epidural fibrosis is the major cause of lower back pain and other complications. Numerous studies have shown that apigenin (API) could treat various fibrotic diseases by regulating various signaling pathways, whereas no study has discussed whether API c...

Citations

... Fibrosis is a progressive disease with excessive deposition of extracellular matrix (ECM) composed of fibrillar collagens, EDTA-fibronectin and SMA-stress fiber [27,[36][37][38][39]. Sustained fibroblast proliferation, activation and migration, generally identified as wound healing response, continually happen after tissue injury [40,41]. Healing by epidural fibrosis instead of regeneration may place burden on spinal cord and nerve root after spinal surgery [42]. ...
... Cryptotanshinone inhibited the expression of cyclin D1, and the phosphorylation of Rb in human rhabdomyosarcoma Rh30 cells induced cell cycle arrest at the G0/G1 phase [28]. In addition, previous studies [21,22,29] also showed that tanshinones could inhibit cell migration [30] through a wide range of signaling pathways, i.e., the HIF-1α-EMT pathway [31], STAT3-CCL2-EMT [32], PI3K/AKT signaling [33], and the PI3K and AMPK-mTOR signaling pathway [34]. However, little is still known about the roles and mechanisms of tanshinones, including dihydrotanshinone I in the regulation of cell adhesion and cell migration [33,[35][36][37][38][39]. ...
... RAD001 engages in the regulation of the PI3K/ AKT/mTOR pathway [68][69][70]. Next, we investigated the relationship between RAD001 and RTactivated PI3K/AKT/mTOR pathway by adding RAD001 after RT. The results revealed that treatment of RAD001 diminished the expression levels of PI3K, p-AKT, p-mTOR, p-S6K1, and p62, and elevated the LC3-II/LC3-I ratio and amount of acidic autophagic vesicles in CC cells. ...
... Surgery-induced extensive epidural fibrosis following laminectomy often results in negative effects on outcome and even causes failed back surgery syndrome (FBSS) [1,2]. Meanwhile, fibroblast proliferation plays an important role in the formation of EF [28]. Following surgical laminectomy, fibroblasts in the bone defect site are activated by inflammatory cytokines and growth factors to produce collagen and extracellular matrix. ...