Wolfgang Guggemos's research while affiliated with Ludwig-Maximilians-University of Munich and other places

Publications (26)

Article
Full-text available
Significance The highly attenuated vaccinia virus MVA is licensed as smallpox vaccine; as a vector it is a component of the approved adenovirus-MVA–based prime-boost vaccine against Ebola virus disease. Here, we provide results from testing the COVID-19 candidate vaccine MVA-SARS-2-S, a poxvirus-based vector vaccine that proceeded to clinical evalu...
Preprint
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The severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) has emerged as the infectious agent causing the pandemic coronavirus disease 2019 (COVID-19) with dramatic consequences for global human health and economics. Previously, we reached clinical evaluation with our vector vaccine based on vaccinia virus MVA against the Middle East...
Article
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An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Article
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Coronavirus disease 2019 (COVID-19) is an acute respiratory tract infection that emerged in late 20191,2. Initial outbreaks in China involved 13.8% cases with severe, and 6.1% with critical courses³. This severe presentation corresponds to the usage of a virus receptor that is expressed predominantly in the lung2,4. By causing an early onset of sev...
Article
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Background This is a report on the high incidence of olfactory dysfunction in COVID-19 patients in the first cohort of COVID-19 patients in Germany (Webasto cluster).Methods Loss of sense of smell and/or taste was reported by 26 of 63 COVID-19 patients (41%), whereas only 31% of the patients experiencing hyposmia had simultaneous symptoms of rhinit...
Preprint
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Background: In coronavirus disease 2019 (COVID-19), current case definitions presume mainly lower respiratory tract infection. However, cases seen outside the epicenter of the epidemic may differ in their overall clinical appearance due to more sensitive case finding. Methods: We studied viral load courses by RT-PCR in oro- and nasopharyngeal swabs...
Article
HintergrundDeutschland verzeichnete 2015 einen Zuwachs des Flüchtlingsstroms auf etwa 1 Mio. Menschen. Die medizinische Versorgung dieser Patientengruppe birgt einige Besonderheiten, die sich von der medizinischen Versorgung der einheimischen Bevölkerung grundlegend unterscheiden. Katastrophale hygienische Bedingungen während der Flucht sowie Mange...
Article
Hintergrund | Rückfallfieber wird in Läuserückfallfieber (louse-borne-relapsing-fever = LBRF) und Zeckenrückfallfieber (tick-borne-relapsing-fever = TBRF) unterteilt. Es wird über 25 Flüchtlinge aus Ost-Afrika mit LBRF berichtet, die an unserem Krankenhaus diagnostiziert und behandelt worden sind. Patienten und Methode | Gemeinsame klinische Aspekt...
Article
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We report 15 imported louse-borne relapsing fever (LBRF) cases in refugees in Bavaria, Germany. One patient died. Epidemiological findings confirmed that all were young males from the Horn of Africa (12 from Somalia), who had similar migration routes converging in Sudan continuing through Libya and Italy. The majority likely acquired their infectio...
Article
Cowpox viruses are orthopoxviruses that may survive in the environment for years. Rodents are regarded as the primary hosts, but transmission to other species has been reported. This report describes a cowpox virus infection in a cat with subsequent transmission to its owner leading to protracted, atypical and severe clinical signs. A young cat pre...
Article
Full-text available
During the last two decades human infections with Plasmodium knowlesi are increasingly diagnosed in South East Asia and have also been reported in travellers. A severe case of imported P. knowlesi infection in a 73-year old German is presented, who had been travelling through Myanmar and Thailand for three weeks. Microscopy showed a parasitaemia of...
Article
History and clinical findings: A 50-year-old man with HIV infection (first diagnosed > 20 years ago) presented at our hospital with fulminant oral mucositis. Antiretroviral therapy (tenofovir, emtricitabine, raltegravir) had been started 2 months ago. Previously he had no opportunistic infections and no other pre-existing illnesses. He had not tra...
Article
Background: The Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus involved in cases and case clusters of severe acute respiratory infection in the Arabian Peninsula, Tunisia, Morocco, France, Italy, Germany, and the UK. We provide a full description of a fatal case of MERS-CoV infection and associated phylogenetic analys...
Article
Zusammenfassung Ein 63-jähriger Rentner wurde 2 Tage nach Rückkehr von einem Indien/Nepal-Urlaub wegen wässriger Diarrhö, Erbrechen sowie Fieber und Zephalgie stationär aufgenommen. Bei der klinischen Untersuchung fiel ein feinfleckig-papulöses Exanthem an Rumpf und Stirn auf. Laborchemisch zeigten sich eine Thrombo- und Leukopenie sowie ein erhöht...
Article
A 63-year-old patient presented to our emergency unit two days after returning from India suffering from watery diarrhea, nausea, fever and headache. On admission we found a maculopapular rash on his trunk and forehead. Laboratory findings revealed a leuko-thrombopenia and elevated levels of CRP and procalcitonin. We started treatment with ciproflo...
Article
A 71-year-old woman had suffered for 6 weeks from fatigue, dry cough and fever. Five years previously breast cancer had been diagnosed and had been successfully treated with subtotal mastectomy and postoperative radiotherapy, as well as aromatase inhibitor until shortly before the present admission. There was no history of travel to tropical countr...
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Anamnese und klinischer Befund: Eine 71-jährige Rentnerin stellte sich wegen seit 6 Wochen zunehmender Abgeschlagenheit mit Reizhusten und subfebrilen Temperaturen vor. Vor 5 Jahren war sie wegen eines Mamma-Karzinoms behandelt worden (subtotale Mastektomie mit postoperativer Radiatio). Bis 3 Monate vor dem aktuellen Beschwerdebeginn erfolgte eine...
Article
A 24-year-old HIV-positive patient was admitted to hospital on account of increasing headache. On admission, a patient with severe headache, nausea and vomiting but without neurologic deficiencies was seen. The diagnosis of a cryptococcal meningoencephalitis could be confirmed by direct detection of cryptococci in the liquor. The patient was treate...
Article
History and Clinical Findings: A 24-year-old HIV-positive patient was admitted to hospital on account of increasing headache. Investigations: On admission, a patient with severe headache, nausea and vomiting but without neurologic deficiencies was seen. The diagnosis of a cryptococcal meningoencephalitis could be confirmed by direct detection of cr...
Article
History: A 40-year-old teacher fell ill one week after returning from a two weeks back-packing trip to Thailand and Laos. He developed high fever, severe headache, myalgias and a conjunctivitis. Investigations: CRP and liver enzymes were elevated. The patient developed acute renal failure. Total leucocyte count was normal but the differential co...
Article
A 40-year-old teacher fell ill one week after returning from a two weeks back-packing trip to Thailand and Laos. He developed high fever, severe headache, myalgias and a conjunctivitis. CRP and liver enzymes were elevated. The patient developed acute renal failure. Total leucocyte count was normal but the differential count showed an extreme left s...
Article
A 49-year-old travel guide fell ill during his return from a 6-week-stay in Vietnam, Myanmar and Thailand. He developed high fever and severe headache. On admission, the body temperature was at 39.5 degrees C with relative bradycardia. A black, crusted skin lesion of approximately 5 mm diameter was noted on the chest wall and was interpreted as an...

Citations

... Although several SARS-CoV-2 vaccines based on MVA have been developed and evaluated in animal models for immunogenicity and efficacy 29,59-62 , there is currently no MVA-based SARS-CoV-2 vaccine approved for routine clinical use. COH04S1 and MVA-SARS-2-S, an MVA vector expressing S alone, are currently the only MVA-based SARS-CoV-2 vaccines that are clinically evaluated 62 . In addition, COH04S1 and a recently developed adenovirus vector approach are currently the only clinically evaluated SARS-CoV-2 vaccines that utilize an antigen combination composed of S and N 63 . ...
... The companies ReiThera, Leukocare and Univercells, using the nonreplicative simian adenoviral vector GRAd32, have developed the GRAd-COV-2 vaccine, which contains the coding sequence of the full-length S glycoprotein, to which only an influenza HA tag (YPYDVPDYA) has been added, to facilitate the detection of antigen expression by commercially available HA antibodies (70). Similarly, the University of Munich, using the vector modified vaccinia virus Ankara (MVA), developed the MVA-SARS-2-S vaccine, with the same characteristics in the S glycoprotein, however, MVA-SARS-2-S encodes a stabilized S glycoprotein by replacing the K986P residues and V987P on top of the HR1 central helix of the S2 subunit (71). Pfizer-BioNTech have successfully developed the BNT162b2 mRNA vaccine, which also consists of the full-length S glycoprotein with the K986P and V987P mutation sites (72). ...
... In another small study of 26 samples with positive cultures, there was no growth after 8 days of onset of symptoms and there was a reduction in the odd ratio of positivity of 37% reduction with each day post symptoms [13]. In much smaller study of nine patients, SARS-CoV-2 PCR was positive for 28 days post development of symptoms and no positive viral cultures after 8 days [14,15]. One study compared Delta and Omicron variants and found that the time to culture negativity was a median of 6 days (interquartile 50 range of 4-8 days) [16]. ...
... Data from some various studies suggest that Covid-19 can lead to hyposmia in about 30% of patients. It is also observed in most patients that the olfactory disorder is not associated with nasal obstruction, so the probable sort of this disorder can be considered neurological [13]. ...
... RNA was extracted using 200 µl and the Viral NA Small Volume Kit and the MagNA Pure 96 system (Roche) [10]. Furthermore, viral cell culture was performed to detect the cytopathic effect of the detected SARS-CoV-2 virus material as a proxy for infectiousness as described before [11]. ...
... (Lauer et al., 2020); a mild infection duration of 6 days (5.5-6.5) (Wölfel et al., 2020); a severe infection duration of 4.5 days (3.5-5.5) (Sanche et al., 2020); a critical infection duration of 6.7 days (4.2-10.4); ...
... Hospital testing strategies vary. While some facilities rely on testing of symptomatic patients only, others combine diagnostic testing with screening because SARS-CoV-2 can also be transmitted by asymptomatic and pre-symptomatic individuals [7][8][9][10]. Depending on the setting, patients are either universally screened on admission [11], or screening is applied to specific patient groups, e.g. ...
... While morbidity and mortality of migrants, which are similar to those of the majority population, are discussed as "healthy migrant effects", there are some peculiarities among asylum seekers [6]. Among other things, there are increased rates of infectious diseases such as tuberculosis, HIV or hepatitis, scabies or measles [7][8][9]. ...
... In the present study, we sought to develop and evaluate a point-of-care test for the serodiagnosis of LBRF by utilizing serum samples from clinical-and culture-confirmed LBRF patients collected in the 1990´s in Ethiopia and during the course of the refugee crisis in 2015 (Fekade et al., 1996;Cutler et al., 2010;Hoch et al., 2015;Seilmaier et al., 2016). For pre-screenings, IgM and IgG immunoreactivities of these serum samples were tested by conventional immunoblotting employing whole cell lysate of B. recurrentis. ...
... In 1985, in the Andes of Peru, at altitudes above 3800 m, 60 cases were reported among the inhabitants of villages infested with lice [3]. In the second half of 2015, more than 40 cases of LBRF were imported into central Europe by migrants [153]. ...