William E. Hull's research while affiliated with German Cancer Research Center and other places

Publications (131)

Article
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Several natural drugs (termed prodrugs) when administered orally undergo transformation by intestinal bacteria, producing metabolites, which may be more active than the parent compound. Mangiferin (I) is reported to have very low bioavailability in the upper gastrointestinal tract and reaches the large intestine, where it may be metabolized by the...
Article
Full-text available
Pits of representative green olive drupes from Italy and Greece were evaluated for their content of phenolic compounds. The major purified polyphenols quantitated in mg/kg in the Italian and Greek pit varieties, respectively, were tyrosol (157 and 523), hydroxytyrosol (3 386 and 1723), vanillin (151 and 83), vanillic acid (58 and 101), phloretic ac...
Article
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Here we show that glioblastoma express high levels of branched-chain amino acid transaminase 1 (BCAT1), the enzyme that initiates the catabolism of branched-chain amino acids (BCAAs). Expression of BCAT1 was exclusive to tumors carrying wild-type isocitrate dehydrogenase 1 (IDH1) and IDH2 genes and was highly correlated with methylation patterns in...
Article
Urea-based inhibitors of the prostate-specific membrane antigen (PSMA) represent low-molecular-weight pepidomimetics showing the ability to image PSMA-expressing prostate tumors. The highly efficient, acyclic Ga(III) chelator N,N'-bis [2-hydroxy-5-(carboxyethyl)benzyl] ethylenediamine-N,N'- diacetic acid (HBED-CC) was introduced as a lipophilic sid...
Article
Longan (Dimocarpus longan Lour, syn. Euphoria longan Lam.) represents an important fruit in Northern Thailand and has significant economic impact. The fruit is either consumed fresh or as commercially prepared dried and canned products. The canning industry in Thailand produces considerable quantities of waste products, in particular Longan seeds....
Article
Methylation of cytidine at dCpdG sequences regulates gene expression and is altered in many chronic inflammatory diseases. Inflammation generates lipid peroxidation (LPO) products which can react with deoxycytidine, deoxyadenosine, and deoxyguanosine in DNA to form pro-mutagenic exocyclic etheno-nucleoside residues. Since 5-methyl-2'-deoxycytidine...
Article
A two-stage pilot intervention study with walnuts was conducted in human volunteers (n = 4) to establish the bioavailability of urolithins which are the terminal end-products of ellagitannin metabolism by the faecal flora. Biosamples (blood, faeces and urine) were evauated for their cancer chemopreventive potential in a range of in vitro assays (DP...
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The EUROCarbDB project is a design study for a technical framework, which provides sophisticated, freely accessible, open-source informatics tools and databases to support glycobiology and glycomic research. EUROCarbDB is a relational database containing glycan structures, their biological context and, when available, primary and interpreted analyt...
Article
Thirty-four polyphenolic substances in methanol extracts of the fruits of Terminalia bellerica, Terminalia chebula and Terminalia horrida, three plants used in Egyptian folk medicine, were initially identified by HPLC-ESI-MS and quantitated by analytical HPLC after column chromatography on Sephadex LH-20. After purification by semi-preparative HPLC...
Article
Anisophyllea dichostyla R. Br. (Anisophylleaceae), is a small shrub which grows widely in regions of the Democratic Republic of Congo (DRC) where its root barks are used in folk medicine for the treatment of many debilitating diseases. In a previous work [Khallouki, F., Haubner, R., Hull, W.E., Erben, G., Spiegelhalder, B., Bartsch, H., Owen, R.W.,...
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There are considerable differences between bacterial and mammalian glycans. In contrast to most eukaryotic carbohydrates, bacterial glycans are often composed of repeating units with diverse functions ranging from structural reinforcement to adhesion, colonization and camouflage. Since bacterial glycans are typically displayed at the cell surface,...
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Supplementary Table 5: Comprehensive list of mammalian disaccharide fragments and their relative abundances.
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Supplementary Table 2: Abundance of basetypes for bacterial and mammalian carbohydrates.
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Supplementary Table 4: Abundances of all monosaccharide pairs found in bacterial and mammalian carbohydrates.
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Supplementary Table 1: Abundance of carbohydrate sequences for various taxonomical classes, orders and families.
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Supplementary Table 6: Comparison of mammalian and bacterial disaccharide fragments and their relative abundances.
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Supplementary Table 3: Abundances of monosaccharide residues found in bacterial and mammalian carbohydrates.
Article
Cystic echinococcosis is a worldwide disease caused by larval stages of the parasite Echinococcus granulosus (canine tapeworm). In clinical practice, staging of cyst development by ultrasonography (US) has allowed treatment options to be tailored to individual patient needs. However, the empirical correlation between cyst morphology and parasite vi...
Article
The two-pulse COSY revamped by asymmetric Z-gradient echo detection (CRAZED) NMR experiment has the basic form 90 degrees -Gdelta-t(rec)-beta-nGdelta-t(rec)-FID, with a phase-encoding gradient pulse G of length delta applied during the evolution time tau for transverse magnetization, readout pulse beta, rephasing gradient nGdelta, and recovery time...
Article
The contents of secondary plant substances in solvent extracts of various byproducts (barks, kernels, peels, and old and young leaves) in a range of Brazilian mango cultivars were identified and quantitated. The results show that the profiles of secondary plant substances such as xanthone C-glycosides, gallotannins, and benzophenones in different b...
Article
Full-text available
The root bark of Anisophyllea dichostyla R. Br. is traditionally used in the Democratic Republic Congo for the treatment of several conditions such as anorexia, fatigue and intestinal infections. We have identified and quantitated several polyphenol antioxidants in the methanol extract of the root bark (120g). The polyphenol content (3.32g/kg) was...
Article
A new class of intracellular contrast agents (CA) for magnetic resonance imaging has been developed, based on Gd(DTPA) with two positively charged amide-linked substituents. Uptake of Gd(DTPA) into cultured tumor cell lines (B16 mouse melanoma, MH3924A Morris hepatoma) was below the detection limit while CA with the melanin-binding pharmacophore 2-...
Article
A method involving the coupling of high-performance liquid chromatography with electrospray ionisation mass spectrometry (HPLC-ESI-MS) for the quantitative determination of the mammalian lignans enterolactone and enterodiol in human blood and urine has been developed. In contrast to techniques previously published, the method allows direct measurem...
Article
2-Deoxy-2-[18 F] fluoro-D-galactose was prepared using gaseous [18F]acetyl hypofluorite or [18F]F2 and tri-O-acetyl-D-galactal as substrate. The product was obtained in a surprisingly high epimeric purity (>90%), even with [18F]F2 as fluorinating reagent, as demonstrated by HPLC and high-resolution 1H- and 19F-NMR spectroscopy. This is the first re...
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Background: Meningiomas are usually benign tumors and cyto-genetically well-characterized. Most tumors show either mono-somy 22 or a diploid karyotype. Progression of meningiomas is correlated with increasing hypodiploidy and the loss of the short arm of chromosome 1. The aim of this study was to assess intra-tumoral patterns of clonal chromosomal...
Article
Although it is already known that Tamarind (Tamarindus indica L.) seeds contain phenolic substances, the individual components of the seeds have not been fully identified and quantitated, and in the case of Tamarind pericarp not reported. Therefore, major polyphenolic compounds were extracted using organic solvents and the metabolites were isolated...
Article
KTCTL-26 and KTCTL-2 are renal cell carcinoma (RCC) lines with high and low expression of P-170 glycoprotein, respectively. Inherent differences between the two cell lines in terms of phosphate metabolites and growth characteristics in culture were examined for possible association with multidrug resistance (MDR). Differences in response to drug tr...
Article
High-field NMR studies at 11.7 T of newly synthesized diastereomeric 1-aza-2-bis(2-chloroethyl)amino-3-oxa-2- oxo-2-phospha-7-thia-bicyclo[4.4.0]decanes (4, 5) and the corresponding [4.3.0]nonanes (6, 7) were carried out by employing a combination of one- and two-dimensional homo- and heteronuclear methods. Unambiguous 31P, 1H and 13C chemical shif...
Article
1[4-13C]Nicotinate was synthesised and used to support the growth of a nicotinate auxotrophic mutant of Pseudomonas putida. 13C-NMR spectroscopy of the isolated urocanase confirmed the efficient incorporation of 13C into C4 of the nicotinamide ring of the tightly bound NAD+ cofactor.2β-([2′-13C]Imidazol-4-yl)propionate was synthesised according to...
Article
Tumor targeting of glycoconjugated antineoplastic agents is a strategy currently under investigation for cancer chemotherapy. We have synthesized the glucosides and galactosides of the clinically established drug hydroxyurea and of mesylglycol, the reactive moiety of the anticancer drug busulfan. Glycosides of hydroxyurea were obtained by carbamoyl...
Article
A convenient method for the functionalization of proteins with DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) has been developed. For this purpose DOTA was converted into a series of different monoreactive activated phenolic esters. The esters were prepared in a single step from commercially available DOTA, using 1-ethyl-3-[3-(dime...
Article
A Sprague-Dawley rat model with DS sarcoma transplanted in the thigh was used to compare transcatheter locoregional i.a. and systemic i.v. administration of 5-fluorouracil (FU) at 12 dose-rate schedules: 25, 50 and 100 mg/kg; bolus, 1, 5 and 24 h infusions. In experiment A tumor (62/67 animals) as well as liver and kidney (56/67 animals) were excis...
Article
Although it is already known that carob fibre contains several classes of polyphenolic substances, a comprehensive analysis of these has not been conducted to date. Therefore, the major polyphenolic compounds were extracted with organic solvents, and, following fractionation by normal-phase column chromatography on silicic acid, their structures we...
Article
Following an i.p. dose of 150 mg x kg(-1) 5-fluorouracil (5-FU), drug uptake and metabolism over a 2-h period were studied by in vivo (19)F magnetic resonance spectroscopy (MRS) for the murine colon carcinoma lines C26-B (5-FU-insensitive; n=11) and C26-10 (5-FU-sensitive; n=15) implanted s.c. in Balb/C mice. Time courses for tumour growth, intrace...
Article
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Because olives represent an important component of the Mediterranean diet, it is necessary to establish unequivocal identification and quantitation of the major potential antioxidant phenolic compounds they contain. The major phenolic antioxidants in two types of brined olives were isolated and purified by semi-preparative high performance liquid c...
Article
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The rat prostate tumour cell line R3327 AT-1 was transfected with a gene coding for a fusion protein comprised of cytosine deaminase (CD from E. coli) and thymidine kinase (TK from Herpes simplex virus, HSV-1). The resulting AT-1/CDglyTK cell line was sensitive to the prodrug 5-fluorocytosine (IC(50) = 78 microM, 96-h incubation) via CD and to ganc...
Article
Targeted fluorescent dyes are of substantial value for the intraoperative delineation of primary tumors and metastatic lesions. For this purpose long-wavelength red light (lambda=550-650 nm) offers advantages because of good tissue penetration and direct visibility. Since somatostatin receptors (SSTR) are overexpressed in a number of tumors, a seri...
Article
5-fluorouracil (5-FU), widely used for chemotherapy of colorectal carcinoma, requires intracellular anabolic conversion to cytotoxic nucleotides and exhibits a narrow therapeutic range with dose-dependent and concentration-dependent effects. 5-FU undergoes extensive metabolic degradation to several catabolites, which are excreted mainly by the kidn...
Article
A series of potential inhibitors of the human DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) were synthesized, characterized in detail by NMR, and tested for their ability to deplete MGMT activity in vitro. The new compounds, omega-[O(6)-R-guan-9-yl]-(CH(2))(n)-beta-d-glucosides with R = benzyl or 4-bromothenyl and omega = n = 2...
Article
In the Mediterranean basin, olive oil, along with fruits, vegetables, and fish, is an important constituent of the diet, and is considered a major factor in preserving a healthy and relatively disease-free population. Epidemiological data show that the Mediterranean diet has significant protective effects against cancer and coronary heart disease....
Article
Several radioiodinated N-(dialkylaminoalkyl)benzamides have been used for planar scintigraphy and single-photon emission computed tomography (SPECT) of melanoma metastases. In a quest for improved melanoma uptake and tissue selectivity, structure-activity studies for N-(2-diethylaminoethyl)benzamides with variation of phenyl substituents were perfo...
Article
The aim of this study was to evaluate the phenolic antioxidant and squalene content in a range of olive and seed oils. A mean of 290 +/- 38 (SEM) mg squalene/100 g was detected. However, while there was a weak significant difference between extra virgin (424 +/- 21 mg/kg) and refined virgin (340 +/- 31 mg/100 g; P<0.05) olive oils, highly significa...
Article
Because olive oil is an important component of the Mediterranean diet, it is necessary to establish unequivocal identification of the major potential antioxidant phenolic compounds it contains. The major phenolic antioxidants in extra virgin olive oil were isolated and purified. Structural analysis was conducted using several spectroscopic techniqu...
Article
In our ongoing studies on the chemoprevention of cancer we have a particular interest in the health benefits of the Mediterranean diet, of which olive oil is a major component. Recent studies have shown that extravirgin olive oil contains an abundance of phenolic antioxidants including simple phenols (hydroxytyrosol, tyrosol), aldehydic secoiridoid...
Article
A [2+2] cycloaddition cap-to-cap C70 dimer with C2h molecular symmetry was synthesized in high yield by pressure treatment of polycrystalline C70 at 1 GPa and 200 °C. It was separated from unreacted monomers by chromatography and characterized by 13C NMR, Raman, and infrared spectroscopy, and other methods. Remarkably, only one isomer was produced...
Article
A series of (hydroxyalkyl)cobalamins, i.e., 1a – d, (HO−(CH2)n−Cbl, n=2 – 5), two diastereoisomeric (2,3-dihydroxypropyl)cobalamins, i.e., 2a,b ([(R)- and (S)-[(HO)2pr]-Cbl) and their diastereoisomeric `base-off' analogues, the (Coβ-2,3-dihydroxypropyl-[1′-O-(p-tolyl)cobamides]) 3a,b ([(R)- and (S)-(HO)2pr]-PTC) were prepared and characterized by t...
Article
Fluoropyrimidine chemotherapy relies on the intracellular anabolic conversion of 5-fluorouracil and the corresponding nucleosides to cytotoxic fluorinated nucleotides (F-Nuctd), such as 5-fluorouridine-5'-triphosphate (FUTP) or 5-fluoro-2'-deoxyuridine-5'-monophosphate (FdUMP), which can be detected by 19F-NMR spectroscopy. We have made 19F-NMR sig...
Article
Fluoropyrimidine chemotherapy relies on the intracellular anabolic conversion of 5-fluorouracil and the corresponding nucleosides to cytotoxic fluorinated nucleotides (F-Nuctd), such as 5-fluorouridine-5′-triphosphate (FUTP) or 5-fluoro-2′-deoxyuridine-5′-monophosphate (FdUMP), which can be detected by 19F-NMR spectroscopy. We have made 19F-NMR sig...
Article
A first-principles quantum molecular dynamics (QMD) method and a bond polarizability model, whose parameters were optimized on the basis of C60 data, have been used to calculate theoretical Raman spectra for four possible low-energy isomers of the odd-numbered dimeric fullerene C119 produced by thermolysis of C60 oxides. Comparison of the calculate...
Article
C120OS has been synthesised by thermal reaction of C120O and sulfur, isolated by HPLC, and characterised by MALDI-TOF-MS, LT-FAB-MS, XPS, Raman, UV-VIS, IR and 13C-NMR spectroscopy; all data are consistent with a molecule of Cs symmetry with the fullerene cages linked by furan- and thiophene-like bridging units.
Article
Approximately 2 micromol of a novel prokaryotic pheromone, involved in starvation-induced aggregation and formation of fruiting bodies by the myxobacterium Stigmatella aurantiaca, were isolated by a large-scale elution procedure. The pheromone was purified by HPLC, and high-resolution MS, IR, 1H-NMR, and 13C-NMR were used to identify the active sub...
Article
Two products, A and B, with the formula C120O2 have been characterized by HPLC, MALDI-TOF MS, FT-Raman, and 13C NMR spectroscopy. C120O2(A) was found as a byproduct accompanying C120O in the solid-phase reaction at 200 °C of mixtures containing approximately a 4:1 ratio of C60 to C60O and traces of C60O2. Raman spectra indicated that A has a furano...
Article
PHIPA 3-10 [13-(4'-iodophenyl)-3-(p-phenylene)tridecanoic acid] is a p-phenylene-bridged, radioiodinated omega-phenyl fatty acid that has recently been developed to study coronary artery disease or cardiomyopathies. Here, we demonstrate that PHIPA 3-10 exhibits the characteristics of a long-chain fatty acid, including its ability to be efficiently...
Article
Full-text available
(Co beta-5'-Deoxyadenosin-5'-yl)-(p-cresolyl)cobamide (Ado-PCC), an analogue of the base-off form of coenzyme-B12 (CoB12), was prepared by alkylation of (Co alpha/beta-cyano/aqua)-(p-cresolyl)cobamide (PCC) with 5'-chloro-5'-deoxyadenosine. The 500 MHz 1H-NMR spectrum of Ado-PCC in D2O at pH 7.4 was completely analyzed using COSY and NOESY two-dime...
Article
The metastatic ESb-MP murine lymphoma in DBN2 mice has been used as a model for investigating metastatic disease and its cure by adoptive immunotherapy (ADI) as monitored by in vivo multislice spin-echo 1H NMR microimaging at 7 T. Isoflurane inhalation anesthesia facilitated long measurement sessions, and respiratory gating with a fiber-optic senso...
Article
The metastatic ESb-MP murine lymphoma in DBA/2 mice has been used as a model for investigating metastatic disease and its cure by adoptive immunotherapy (ADI) as monitored by in vivo multislice spin-echo 1H NMR microimaging at 7 T. isoflurane inhalation anesthesia facilitated long measurement sessions, and respiratory gating with a fiber-optic sens...
Article
Computational methods are intensively applied to explore the conformational space of oligosaccharides. In this report, the current modelling protocols for the conformational analysis of carbohydrates are reviewed. Approaches which need direct input of experimental data from NMR experiments (distance mapping and restrained molecular dynamics), vario...
Article
Thermolysis of C120O at 550-600°C yielded a soluble product fraction containing C60, C119 and higher-mass species. Using a two-step HPLC procedure two species, both with molecular weight corresponding to C119, were isolated in 3.5% and 2% yield. The 125.76 MHz 13C-NMR spectrum of the latter more stable product obtained from 13C enriched samples pro...
Article
Full-text available
Thermolysis of C120O at 550–600°C yielded a soluble product fraction containing C60, C119 and higher-mass species. Using a two-step HPLC procedure two species, both with molecular weight corresponding to C119, were isolated in 3.5% and 2% yield. The 125.76 MHz 13C-NMR spectrum of the latter more stable product obtained from 13C enriched samples pro...
Article
The development of an effective radiopharmaceutical with affinity for malignant melanoma has been a research goal for some time. The early detection of melanoma metastases would greatly improve the therapy outcome for this disease. This article describes the synthesis of radioiodinated IMBA, N-(2-diethylaminoethyl)-3-[123I/131I]iodo-4-methoxybenzam...
Article
The intradermal ESb-MP murine T-cell lymphoma in syngeneic DBA/2 mice has been used as a model for adoptive immunotherapy (ADI). Cultured ESb-MP cells were characterized in suspension by 31P-NMR spectroscopy (MRS) at 11.7 T, and solid primary tumors were examined by 31P-MRS in vivo at 7.0 Tesla using surface-coil techniques. Growing tumors containe...
Article
Full-text available
31P magnetic resonance spectroscopy has been used to determine phosphate metabolite profiles in five human tumor cell lines in culture and as solid tumor xenografts in nude mice. Significant differences between cell lines, in particular in their phospholipid metabolite levels, were observed. The largest differences between metabolite profiles in vi...
Article
Full-text available
β-D-Glucosylisophosphoramide mustard (β-DGlc-IPM) is a new, potential chemotherapeutic agent currently under investigation. Its pharmacokinetics in plasma and elimination of the parent drug and its metabolites via urine, bile, and exhaled air were studied in female Sprague-Dawley rats after bolus injection of 315 mg/kg. Typically, the drug’s dispos...
Article
A cellular cancer therapy is described with unique efficiency even in late-stage disease. in situ activated tumor-immune T cells, induced in allogeneic, tumor-resistant, MHC identical but superantigen different donor mice (B10.D2) could transfer strong graft-versus-leukemia (GvL) effects accompanied by only mild graft-versus-host (GvH) reactivity....
Article
Previous investigations using low-field 31P-NMR of the isolated perfused kidney demonstrated sequential changes in phosphate components during ischemia. The purpose of this study was: 1) to use high-field 31P-NMR spectroscopy (202 MHz at 11.7 Tesla) of the rat kidney ex vivo to quantitate renal pH and NTP and other phosphate metabolite levels durin...
Chapter
Aufgabe der Zentralbibliothek ist die Sammlung, Erschließung und Bereitstellung der für das Deutsche Krebsforschungszentrum relevanten wissenschaftlichen Literatur. Daneben betreut sie die acht den Forschungsschwerpunkten zugeordneten dezentralen Bibliotheken.
Article
A convergent synthesis of the five novel analogues 1a–e of (5′-deoxyadenosin-5′-yl)cobalamin ( = ‘adenosylcobalamin’) is described. The analogues 1a–e carry oligomethylene chains (C3–C7) inserted between the central Co-atom and the 5′-O-atom of the adenosine moiety and are thought to mimick the transition-state geometry in coenzyme-B12-catalyzed re...
Article
Full-text available
High-resolution 31P NMR spectroscopy at 11.7 T was used to examine the influence of medium formulation (medium and serum type, and concentrations of glucose and inositol) on the cellular phosphate metabolism of CX-1 cells, a human colon cancer cell line derived from HT-29 cells. Striking differences in the 31P spectra of harvested CX-1 cells were o...
Article
Two peptides from the C-terminal region of the major beta-tubulin isotype (400-436 and 400-445) that include the critical areas for interaction with MAP2 and tau were examined to determine their conformations in aqueous solution. Despite a high theoretical potential for alpha-helix formation, CD spectroscopy showed that these peptides consisted pri...
Article
This report describes a noninvasive 1H-NMR method for measuring absolute temperatures (+/- 0.2 degrees C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature-independent shift of one of the components of the biological medium...
Article
The application of 31P magnetic resonance (MR) to provide a noninvasive evaluation of renal phosphorus metabolism is well established [7, 13, 14]. In a variety of 31P MR studies of excised or perfused kidneys initial attention has been given to determining the time course of nucleotide (NTP + NDP) depletion (sum of all nucleoside 5’-tri- and diphos...
Book
The purpose of this book is to provide some basic information regarding the fundamentals of magnetic resonance (MR) for all those who are less familiar with the technique and interested in MR spectroscopy and its possible applications in research and clinical practice. It will stimulate the reader to look more closely at the various topics covered...
Chapter
The purpose of this introductory chapter is to provide some basic information regarding the fundamentals of magnetic resonance (MR) for those readers who are less familiar with the technique. Emphasis here is on MR spectroscopy and its possible applications in research and clinical practice, hopefully stimulating the reader to look more closely at...
Article
To investigate whether bifunctional ligands containing chelating structures other than EDTA and DTPA and metallic radiotracers other than 111In will reduce the non-specific radioactivity uptake in the liver during immunoscintigraphy, we synthetized an isothiocyanato-substituted phenolic polyaminocarboxylic acid (HBED-CI) for labeling of MAbs with 6...
Article
As an adjunct to the monitoring of intracellular high-energy phosphorus metabolites by 31P NMR1 for assessment of renal viability, a study of vasculature of preserved kidneys was performed by Image-Guided Volume Selective 19F MR Spectroscopy. 1H and 19F of the same preserved rat and porcine kidneys were obtained. The technique enabled the analysis...
Article
Full-text available
The effects of 2-deoxy-2-fluoro-D-galactose (dGalF) on N- and O-glycosylation of proteins was studied in rat hepatocyte primary cultures and in human monocytes. In hepatocytes, dGalF at concentrations of 1 mM or higher completely inhibited N-glycosylation of alpha 1-antitrypsin and alpha 1-acid glycoprotein, whereas 4 mM-2-deoxy-D-galactose (dGal)...
Article
Recently established methods as Positron-Emission-Tomography (PET) and Magnetic Resonance Spectroscopy (MRS) enable new approaches to investigate tumor metabolism. PET allows to determine vascular perfusion of tumorous areas and an assessment on a quantitative basis. Furthermore, the accumulation of cytostatic drugs within areas of tumor may be mea...
Article
The influence of infusion time and dose on the anticancer efficacy of 5-fluoro-2'-deoxyuridine (FdUrd) was investigated using a locoregional therapy model: Novikoff hepatoma transplanted i.m. into the thigh of Wistar rats and FdUrd infusion via a catheter implanted in the femoral artery. In experiment A the FdUrd dose (five daily doses of 12, 19 an...
Chapter
31 P NMR is of particular interest for tumor investigations, since various neoplasms can differ from normal cells in pH, energy metabolism, and phospholipid turnover (proliferation rate). Human tumor cells can be studied as cultures in an appropriate matrix or as solid tumors (xenografts) in the nude mouse.
Article
http://deepblue.lib.umich.edu/bitstream/handle/2027.42/47534/432_2005_Article_BF01625409.pdf
Article
1. [4-13C]Nicotinate was synthesised and used to support the growth of a nicotinate auxotrophic mutant of Pseudomonas putida. 13C-NMR spectroscopy of the isolated urocanase confirmed the efficient incorporation of 13C into C4 of the nicotinamide ring of the tightly bound NAD+ cofactor. 2. beta-[( 2'-13C]Imidazol-4-yl)propionate was synthesised acco...
Article
Full-text available
Gastrointestinal malignancy may spread to peritoneal surfaces in the absence of lymphatic or hematogenous metastases. To treat peritoneal carcinomatosis, a uniformly lethal disease process, extensive cytoreductive surgery and i.p. chemotherapy were combined. Early postoperative i.p. chemotherapy was instilled in the first few days after the surgica...
Article
The synthetic d-galactose analog 2-deoxy-2-fluoro-d-galactose (dGalF) offers unique advantages for studies of the d-galactose pathway by non-invasive techniques using ¹⁹F-NMR spectroscopy or positron emission from the ¹⁸F-labeled compound. The metabolism of 2-deoxy-2-fluoro-d-galactose was studied in rodents using the unlabeled, the ¹⁸F-labeled, an...
Article
202 MHz 31P NMR (11.7 T) was used to study the effects of culture medium pH on the levels of phosphate metabolites in three human tumor cell lines (XP29MAmal, a malignant xeroderma pigmentosum; CX-1, a colon carcinoma; KB, a squamous cell carcinoma of the oral cavity). Cells were cultured in Roux flasks in HAM's F-12 medium, and the pH was varied w...
Article
In this study we examined the cytostatic compounds 5 FU and FUDR which are most frequently used in regional chemotherapy for any incidence of hepatobiliary toxicity in animals. For this we compared the intraarterial as well as the intraportal application. Differences between the treatment groups were found in the biliary extraction of these two cyt...