Wenling Wang's research while affiliated with National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention and other places

Publications (70)

Article
Introduction: The first imported case of monkeypox (MPX) from the mainland of China was reported in September 2022. Herein, the study reports the isolation and characterization of MPX virus (MPXV) in this case. Methods: Clinical specimens including skin blister fluid, oropharyngeal and nasopharyngeal swabs, and blood were collected and inoculate...
Article
On September 16, 2022, the first imported monkeypox case was reported in mainland China. Laboratory tests including nucleic acid detection were carried out in Chongqing Nan'an Centre for Disease Control and Prevention and Chongqing Center for Disease Control and Prevention. After that, monkeypox virus was isolated by the Institute for Virus Disease...
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Background BBIBP-CorV and CoronaVac inactivated COVID-19 vaccines are widely-used, World Health Organization-emergency-listed vaccines. Understanding antibody level changes over time after vaccination is important for booster dose policies. We evaluated neutralizing antibody (nAb) titers and associated factors for the first 12 months after primary-...
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Background To mitigate a national shortage of WIBP-CorV COVID-19 vaccine, China’s regulator approved administering BBIBP-CorV after WIBP-CorV for completion of a primary series. In a pragmatic observational study, we compared immunogenicity and safety of a primary series of WIBP-CorV followed by BBIBP-CorV with a primary series of two doses of BBIB...
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The coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a public health emergency of international concern, and an effective vaccine is urgently needed to control the pandemic. Envelope (E) and membrane (M) proteins are highly conserved structural proteins among SARS-CoV...
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Although inoculation of COVID-19 vaccines has rolled out globally, there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries. Here, we report on the development of a highly efficacious mRNA vaccine, SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike...
Article
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has stimulated the search for effective drugs for its prevention and treatment. Natural products are an important source for new drug discovery. Here, we report that, NK007(S,R), a tylophorine malate, displays high antiviral activ...
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Background: Although SARS-CoV-2 infection often causes milder symptoms in children and adolescents, young people might still play a key part in SARS-CoV-2 transmission. An efficacious vaccine for children and adolescents could therefore assist pandemic control. For further evaluation of the inactivated COVID-19 vaccine candidate BBIBP-CorV, we ass...
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Chikungunya fever is an acute infectious disease that is mediated by the mosquito-transmitted chikungunya virus (CHIKV), for which no licensed vaccines are currently available. Here, we explored several immunization protocols and investigated their immunity and protective effects in mice, with DNA- and virus-like particle (VLP)- vaccines, both alon...
Article
The on-going global pandemic of coronavirus disease 2019 (COVID-19) caused by a novel coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been underway for about 11 months. Through November 20, 2020, 51 detection kits for SARS-CoV-2 nucleic acids (24 kits), antibodies (25 kits), or antigens (2 kits) have been approve...
Article
Background: Middle East respiratory syndrome (MERS) remains of global public health concern. Dromedary camels are the source of zoonotic infection. Over 70% of MERS coronavirus (MERS-CoV)-infected dromedaries are found in Africa but no zoonotic disease has been reported in Africa. We aimed to understand whether individuals with exposure to dromeda...
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Background The ongoing COVID-19 pandemic warrants accelerated efforts to test vaccine candidates. We aimed to assess the safety and immunogenicity of an inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine candidate, BBIBP-CorV, in humans. Methods We did a randomised, double-blind, placebo-controlled, phase 1/2 trial at...
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The recent pandemic of Zika virus (ZIKV) infections highlights the urgent need for the development of a safe and efficacious ZIKV vaccine. We previously demonstrated that robust humoral and cellular immunity was elicited in BALB/c mice by ZIKV DNA vaccine encoding the precursor membrane (prM) and envelope (E) proteins while the protective efficacie...
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SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but the underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize the replication dynamics, cell tropism and morphogenesis of SARS-CoV-2 in organotypic human airway epithelial (HAE) cultures. SARS-CoV-2 replicate...
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Background The novel coronavirus (SARS-CoV-2) shares approximately 80% whole genome sequence identity and 66% spike (S) protein identity with that of SARS-CoV. The cross-neutralization between these viruses is currently not well-defined. Methods Here, by using the live SARS-CoV-2 virus infection assay as well as HIV-1 based pseudotyped-virus carry...
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With the development of the COVID-19 epidemic, there is an urgent need to establish a system for determining the effectiveness and neutralizing activity of vaccine candidates in biosafety level 2 (BSL-2) facilities. Previously, researchers had developed a pseudotyped virus system for SARS-CoV and MERS-CoV, based on HIV-1 core, bearing virus spike p...
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Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) caused the corona virus disease 2019 (COVID-19) cases in China and has become a public health emergency of international concern1. Because angiotensin-converting enzyme 2 (ACE2) is the cell entry receptor of SARS-CoV5, we used transgenic mice bearing human ACE2 and infected with SARS-CoV-2 to stu...
Article
What is already known on this topic?: A novel human coronavirus, known as SARS-CoV-2 or 2019-nCoV, is the causative agent of the coronavirus disease 2019 (COVID-19). We have released the primers and probes of real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for the laboratory detection of COVID-19 infection. What is adde...
Article
What is already known on this topic? Coronavirus disease 2019 (COVID-19), a disease caused by a novel human coronavirus named the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or COVID-19 virus, was reported in December 2019. Complete genomes of the COVID-19 virus from clinical samples using next generation sequencing (NGS) have bee...
Article
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) threatens global public health. The development of a vaccine is urgently needed for the prevention and control of COVID-19. Here, we report the pilot-scale production of an inactivated SARS-CoV-2 vaccine candidate (BBIBP-CorV) tha...
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Background Since December 2019, an outbreak of the Corona Virus disease 2019 (COVID‐19) caused by severe acute respiratory syndrome coronavirus (SARS‐CoV‐2) in Wuhan, China, has become a public health emergency of international concern. The high fatality of aged cases caused by SARS‐CoV‐2 was a need to explore the possible age‐related phenomena wit...
Preprint
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously designated as 2019-nCoV) outbreak has caused global concern1. Currently, there are no clinically approved specific drugs or vaccines available for this virus. The viral polymerase is a promising target for developing broad- spectrum antiviral drugs. Here, based on t...
Article
An epidemic of respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began in China and has spread to other countries.¹ Real-time reverse transcriptase–polymerase chain reaction (rRT-PCR) of nasopharyngeal swabs typically has been used to confirm the clinical diagnosis.² However, whether the virus can be detecte...
Preprint
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Background: The 2019 novel coronavirus (2019-nCoV or SARS-CoV-2) has spread more rapidly than any other betacoronavirus including SARS-CoV and MERS-CoV. However, the mechanisms responsible for infection and molecular evolution of this virus remained unclear. Methods: We collected and analyzed 120 genomic sequences of 2019-nCoV including 11 novel ge...
Preprint
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2019-nCoV caused pneumonia cases in China has become a public health emergency of international concern (PHEIC). The first priority for prevention and treatment of the disease is to find the pathogenicity of 2019-nCoV in vivo. Weight loss and virus replication were detected in infected-hACE2 mice. The typical histopathology was interstitial pneumon...
Article
Background: In late December, 2019, patients presenting with viral pneumonia due to an unidentified microbial agent were reported in Wuhan, China. A novel coronavirus was subsequently identified as the causative pathogen, provisionally named 2019 novel coronavirus (2019-nCoV). As of Jan 26, 2020, more than 2000 cases of 2019-nCoV infection have be...
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In December 2019, a cluster of patients with pneumonia of unknown cause was linked to a seafood wholesale market in Wuhan, China. A previously unknown betacoronavirus was discovered through the use of unbiased sequencing in samples from patients with pneumonia. Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV,...
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The Middle East respiratory syndrome (MERS) is a lethal zoonosis caused by MERS coronavirus (MERS-CoV) and poses a significant threat to public health worldwide. Therefore, a rapid, sensitive, and specific serologic test for detecting anti-MERS-CoV antibodies in both humans and animals is urgently needed for the successful management of this illnes...
Article
Zika virus (ZIKV) causes rash, moderate fever, conjunctivitis, and arthralgia, and has serious connection with neurological complications; therefore, it is a major threat to public health. A rapid and supersensitive method for detecting anti-ZIKV antibodies in humans and animals is thus urgently required. Here, we report an NS1-based luciferase imm...
Article
Conventional influenza vaccines need to be designed and manufactured yearly. However, they occasionally provide poor protection owing to antigenic mismatch. Hence, there is an urgent need to develop universal vaccines against influenza virus. Using nucleoprotein (NP) and extracellular domain of matrix protein 2 (M2e) genes from the influenza A viru...
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Monkeypox virus is a zoonotic disease endemic to Africa. In 2017, we confirmed a case of human monkeypox virus in Sierra Leone by molecular and serologic methods. Sequencing analysis indicated the virus belongs to the West African clade and data suggest it was likely transmitted by wild animals.
Article
Currently, there is no approved therapy to treat coronavirus infection; therefore, broad-spectrum inhibitors of emerging and endemic CoVs are needed. Based on our high-throughput screening assay using a compound library, we identified seven compounds with broad-spectrum efficacy against the replication of four CoVs in vitro . Additionally, one comp...
Article
The Zika virus (ZIKV) and poxvirus infection are considered as public health emergencies, necessitating the development of effective vaccines. Here, we report novel recombinant DNA-based and non-replicating vaccinia virus (NTV)-based vaccine candidates that express the precursor membrane-envelope (prME) or envelope (E) glycoproteins of ZIKV. After...
Article
Therapeutic vaccination is a promising strategy for controlling chronic hepatitis B virus (HBV). Here, we tested whether several novel vaccination strategies could be used to induce HBV-specific adaptive immune responses and control/eradicate HBV in a mouse model. Robust HBV antigen-specific antibody responses were elicited by several vaccination s...
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Background: The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory infection with a high (~35%) mortality rate. Neutralizing antibodies targeting the spike of MERS-CoV have been shown to be a therapeutic option for treatment of lethal disease. Methods: We describe the germline diversity and neutralizing activity of...
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The persistent public health threat of infection with Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the need for an effective and safe MERS-CoV vaccine. In this study, we prepared and vaccinated mice with either a Spike (S) protein or inactivated whole MERS-CoV (IV) with a combined adjuvant (alum+CpG) as a vaccine formulation....
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Emerging Microbes and Infections (EMI) is a new open access, fully peer-reviewed journal that will publish the best and most interesting research in emerging microbes and infectious disease.
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Middle East respiratory syndrome coronavirus (MERS-CoV) has caused fatal infections, some through hospital-acquired transmission, in affected regions since its emergence in 2012. Although the virus is not pandemic among humans, it poses a great threat to public health due to its zoonotic origin. Thus, both preventative and therapeutic countermeasur...
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The first imported case of Middle East respiratory syndrome (MERS) in China recently occurred, allowing for the characterization of antibody titers in a series of the patient’s sera using the following methods based on recombinant viral structural antigens: inactivated MERS coronavirus (MERS-CoV) enzyme-linked immunosorbent assay (ELISA), recombina...
Article
We aimed to develop a novel laboratory assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) infection .Several novel multiplex real-time RT-PCR assays were developed using the upE,ORF1 band N2genes of MERS-CoV as targets; the novel assays were compared with previous monoplex real-time RT-PCR assays. For validation, we...
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Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel and highly pathogenic human coronavirus and has quickly spread to other countries in the Middle East, Europe, North Africa and Asia since 2012. Previous studies have shown that MERS-CoV ORF4b antagonizes the early antiviral alpha/beta interferon (IFN-α/β) response, which may signifi...
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Unlabelled: The Middle East respiratory syndrome coronavirus (MERS-CoV) causes a severe acute respiratory tract infection with a high fatality rate in humans. Coronaviruses are capable of infecting multiple species and can evolve rapidly through recombination events. Here, we report the complete genomic sequence analysis of a MERS-CoV strain impor...
Article
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On 26 May 2015, an imported Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in Guangdong Province, China, and found to be closely related to the MERS-CoV strain prevalent in South Korea. The full genome of the ChinaGD01 strain was sequenced and analyzed to investigate the epidemiology and evolution of MERS-CoV circulating in...
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To investigate the genetic character and origin of the first imported infection case of middle East respiratory syndrome coronavirus (named as MERS-CoV_China GD01), RNA was extracted from swabs of this patient followed by RT-PCR amplification. All coding gene of structural (S, E, M, E) and accessory (ORF3, ORF4a, ORF4b, ORF5, ORF8b) proteins were s...
Article
The conventional HA and NA-based influenza vaccines need to be updated most years, and are ineffective if the glycoprotein HA of the vaccine strains is a mismatch to that of the epidemic strain. Universal vaccines targeting conserved viral components might provide cross protection, and thus complement and improve conventional vaccines. In this stud...
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The highly conserved internal nucleoprotein (NP) is a promising antigen to develop a universal influenza A virus vaccine. In this study, mice were injected intramuscularly with Escherichia coli-derived NP protein alone or in combination with adjuvant alum (Al(OH)3), CpG or both. The results showed that the NP protein formulated with adjuvant was ef...
Article
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Background Immunity to conserved viral antigens is an attractive approach to develop a universal vaccine against epidemic and pandemic influenza. A nucleoprotein (NP)-based vaccine has been explored and preliminary studies have shown promise. However, no study has explored the immunity and cross-protective efficacy of recombinant NP derived from Es...
Data
The study of the immunogenicity of NM2e in pre-production in mice. The pre-production fermentation and purification of NM2e were finished in SINOVAC BIOTECH CO.,LTD., Beijing, China. The concentration of endotoxin in NM2e protein was decreased to 250 EU/mg after the purification. Mice were immunized with 10 µg of NM2e (G3), 10 µg of NM2e formulated...
Data
Protective efficacy of immunization with the formulation of NM2e in pre-production in mice. Immunized mice (n = 15) were challenged with 30 LD50 of influenza virus A/Brisbane/59/2007(H1N1)-like (MA) on day 38, then they were monitored daily to detect morbidity (left) and mortality (right). *, p≤0.05; **, p≤0.01; ***, p≤0.001. (TIF)
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The 23-amino acid extracellular domain of matrix 2 protein (M2e) and the internal nucleoprotein (NP) of influenza are highly conserved among viruses and thus are promising candidate antigens for the development of a universal influenza vaccine. Various M2e- or NP-based DNA or viral vector vaccines have been shown to have high immunogenicity; howeve...
Article
We previous reported the development of novel hepatitis B virus(HBV) vaccine containing the surface antigen(S) plus PreS1 fusion derived from Chinese hamster ovary (CHO) cells system. In this study, we analyzed the impact of different adjuvants on immunogenicity of the HBV particle vaccine in Balb/C mice, including alum alone, CpG oligodeoxynucleot...
Article
邓瑶; 孟昕; 许洪林; 王世峰; 陆柔剑; 王文玲; 谷淑燕; 阮力

Citations

... The cycle threshold value was 31.95 (Table 2). We also detected the virus load in different sample types of one monkey-pox case found in Chongqing, China in 2022 [30]. The cycle threshold value of blister fluid, oropharyngeal swabs, nasopharyngeal swab, and whole blood was 22.39, 29.46, 34.09, 35.45, respectively ( Table 2). ...
... Furthermore, the epitopebased vaccines could help to overcome challenges such as genetic variations, antigenic shift and antigenic drift. Previously, various epitope-based vaccine candidates against pathogens such as bacteria, viruses, fungi, parasites and cancers have been designed using an in silico immunoinformatics approach [23,42,[78][79][80][81][82][83][84][85][86][87][88]. Recently, an MEV showing a T-cell response for Q fever in cynomolgus macaques has been reported [79]. ...
... 28 As the most wellstudied virus, TMV is often used as a model virus to develop other antiviral agents, such as anti-HIV and anti-SARS-CoV-2 agents. 29,30 In previous work, we have found a series of MNPinspired antiviral candidates based on TMV. 31−33 Taking into account the above findings, laurene was selected as the parent structure. ...
... Vaccines are commonly considered as effective agents for preventing the spread of pandemic diseases. Vaccines have been approved and used in many countries, including vaccines based on mRNA 441,442 , recombinant adenoviral vectors 443,444 , and inactivated vaccines 445,446 . Peptide vaccines have certain advantages, such as high specificity, good safety, and easier production, and have thus become an active research area in development of vaccines against SARS-CoV-2 [447][448][449] . ...
... 11.18.517095 doi: bioRxiv preprint In this study, we developed an HBV mRNA vaccine using our well-established platform 7 . The HBsAg-encoded mRNA sequence was designed using a proprietary artificial intelligence-based algorithm to ensure a high translation efficiency 8 . ...
... The peptides were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 50 mg/ml and stored at -80°C. The experiment was conducted as described previously (22). ...
... NAT method exhibits the advantages of promising sensitivity and specificity for viral detection. However, the accuracy of the test also depends on the sample quality, experimental factors, and kit performance [20]. In this study, we compared two commercial kits (Maccura and Sansure) for RT-PCR detection of SARS-CoV-2 nucleic acids. ...
... We collected data through a survey questionnaires containing questions related to socio-demographic characteristics, comorbidities, previous COVID-19 positive tests and dates, the name of the received vaccine, post-vaccination local signs and symptoms (e.g., pain, swelling, redness, tenderness, itch, warmth, and swollen armpit glands), systemic signs and symptoms (e.g., headache, arthralgia, myalgia, chest pain, fatigue, fever, shiver and chills, diarrhoea, rash, leg swelling, palpitation, sleeplessness, nausea and vomiting, anaphylaxis), the onset and lasting time of signs and symptoms and their severity, and the method of their treatment (self-limiting, taking drugs, hospitalization). We constructed this survey questionnaire using previous reports and publications related to COVID-19 vaccine evaluations and clinical trials, and then it was reviewed and assessed by five expert healthcare professionals to confirm its readability and validity [14,22,23]. ...
... In particular, drinking camel urine alone or mixed with milk is a widely renowned practice within the scope of folk medicine [26]. This habit has frequently been described in Arabian countries, especially among Bedouin tribes with a strong heritage from the Muslim religion [27,28], among which the urine of virgin female camels is the most appreciated [29]. ...
... Compared with the PRNT assay, the SFVNT shortened the turnaround time by several days and increased the testing capacity to high throughput. Another attractive surrogate system to measure neutralizing antibodies at BSL-2 laboratories for vaccine and antibody development [4,9] is SARS-CoV-2 pseudovirus that expresses and presents SARS-CoV-2 spike proteins on the surface of a non-coronavirus [10,11], such as human immunodeficiency virus (HIV-1) [12], murine leukemia virus [13], or vesicular stomatitis virus [14]. However, several intrinsic limitations of the SARS-CoV-2 pseudovirus should be considered. ...