Wen-Jing Sun's research while affiliated with Jiangsu University and other places

Publications (22)

Article
β-1,3-Glucan synthases play key roles in glucan synthesis, cell wall assembly, and growth of fungi. However, their multi-transmembrane domains (over 14 TMHs) and large molecular masses (over 100 kDa) significantly hamper understanding of their catalytic characteristics and mechanisms. In the present study, the 5841-bp gene CMGLS encoding the 221.7...
Article
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β-1,3-Glucans are well-known biological and health-promoting compounds in edible fungi. Our previous results characterized a glucan synthase gene (GFGLS) of Grifola frondosa for the first time to understand its role in mycelial growth and glucan biosynthesis. In the present study, we identified and functionally reannotated another glucan synthase g...
Article
Fungal glucans play a key role to provide energy, support cell structure, and present biological functions. However, the biosynthetic machineries of fungal glucan chains remain to be elucidated. Hence, we aimed to elucidate the biochemical and catalytic characteristics of a novel glucan synthesis-associated enzyme uridine diphosphate (UDP)-glycosyl...
Article
In the present study, effects of maturity stage on structural characteristics and biosynthesis/hydrolysis-associated genes expression of glucans from Volvariella volvacea fruit body were well investigated. Elongation and pileus expansion stages decreased total soluble carbohydrate and protein contents to 17.09 mg/g and 8.33 mg/g, and significantly...
Article
Grifola frondosa polysaccharides, especially β-glucans, showed the significant antitumor, hypoglycemic, and immune-stimulating activities. In the present study, a predominant regulatory subunit gfRho1p of β-1,3-glucan synthase in G. frondosa was identified with a molecular weight of 20.79 kDa and coded by a putative 648-bp small GTPase gene gfRho1....
Article
To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated. Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were construct...
Article
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A two-stage semi-continuous strategy for producing 2-keto-gluconic acid (2KGA) by Pseudomonas plecoglossicida JUIM01 from rice starch hydrolyzate (RSH) has been developed. The initial glucose concentration (140 g/L) was selected for first-stage fermentation due to its highest 2KGA productivity of 7.58 g/(L⋅h), cell weight of 3.91 g/L, and residual...
Article
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The membrane-bound gluconate dehydrogenase (mGADH) is a critical enzyme for 2-keto-d-gluconic acid (2KGA) production in Pseudomonas plecoglossicida JUIM01. The purified native flavin adenine dinucleotide-dependent mGADH (FAD-mGADH) was consisted of a gamma subunit, a flavoprotein subunit, and a cytochrome c subunit with molecular mass of ~ 27, 65,...
Article
Glucan synthase (GLS) gene is known to be involved in the fungal biosynthesis of cell wall, differentiation and growth. In the present study, a glucan synthase gene (GFGLS) in the edible mushroom Grifola frondosa with a full sequence of 5927 bp encoding a total of 1781 amino acids was cloned and characterized for the first time. GFGLSp is a membran...
Article
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The aims of the present study were to optimize the operational parameters to maximize the yield of ultrasound-assisted polysaccharide extraction from Volvariella volvacea (straw mushroom) fruiting bodies by using for the first time one-factor-at-a-time and three-level Box-Behnken factorial designs. A maximum polysaccharide yield of 8.28 ± 0.23% was...
Article
The membrane-bound glucose dehydrogenase (mGDH) is a rate-limiting enzyme for the industrial production of 2-keto-d-gluconic acid (2KGA) from glucose. In this study, mGDH was firstly purified from a 2KGA industrial producing strain Pseudomonas plecoglossicida JUIM01. The purified mGDH exhibited a specific activity of 16.85 U/mg and was identified a...
Article
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Background Mushroom showed pellet, clump and/or filamentous mycelial morphologies during submerged fermentation. Addition of microparticles including Talc (magnesium silicate), aluminum oxide and titanium oxide could control mycelial morphologies to improve mycelia growth and secondary metabolites production. Here, effect of microparticle Talc (45...
Article
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The present study describes the improved mycelia and exo-polymer production under control of Grifola frondosa morphology by changing the aeration rate and agitation intensity in a 25-L stirred fermentor. The aeration rate of 1.0 vvm yielded a highest mycelia biomass of 24.754 g/L with the lowest pellet percentage of 20.5 %. The maximum exo-polymer...
Article
HEG-5, a novel glycoprotein with hemagglutinating activity, was firstly isolated and purified from the cultured mycelia of Hericium erinaceus CZ-2. SDS-PAGE, Native-PAGE and MALDI-TOF-MS proved that HEG-5 was a single band with the molecular weight of approximately 14.4 kDa. HEG-5 had the protein: polysaccharide ratio of approximately 10: 1 (%/%) a...
Article
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D-isoascorbic acid is a food antioxidant additive and used in accordance with Good Manufacturing Practice (GMP). High solubility in water (about 150 g/L at 25[degree sign]C) reduces its effectiveness in stabilizing fats and oils. Our research group had successfully synthesized D-isoascorbyl palmitate using immobilized lipase Novozym 435 as a biocat...
Article
GFPS1b is an antitumor glycoprotein isolated and purified from the mycelia of Grifola frondosa GF9801. In the present study, freeze drying and hot air drying of GFPS1b were compared according to their drying kinetics, functional/conformational/topographical changes, and anti-proliferative activities for the first time. UV spectroscopy, FT-IR, SEM,...
Article
A continuous fermentation process for 2-keto-gluconic acid (2KGA) production from cheap raw material corn starch hydrolysate was developed using the strain Pseudomonas fluorescens AR4. The dilution rate and feeding glucose concentration had a significant effect on the cell concentrations, glucose utilization and 2KGA production performance. The opt...
Article
A continuous conversion process of rice starch hydrolysate to 2-keto-D-gluconic acid (2KGA) by Arthrobacter globiformis C224 was developed. Its feasibility for industrial application was also evaluated. Results showed that the initial cell concentration exceeding 1.25 g/L met the continuous 2KGA production at a stable dilution rate and media compos...
Article
Full-text available
Isoascorbic acid is a stereoisomer of L-ascorbic acid, and widely used as a food antioxidant. However, its highly hydrophilic behavior prevents its application in cosmetics or fats and oils-based foods. To overcome this problem, D-isoascorbyl palmitate was synthesized in the present study for improving the isoascorbic acid's oil solubility with an...
Article
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Bacteriophages have the destructive damage on the industrial bioprocess. 2-Keto-gluconic acid (2KGA) producing bacteria had also been attacked and lysed by bacteriophages which lowered the glucose consumption and 2KGA yield and even stopped the fermentation process. In this study, we presented the characteristics of a novel virulent bacteriophage s...
Article
2-Keto-gluconic acid (2KGA) was produced in a semi-continuous process using Pseudomonas fluorescens AR4 and rice starch hydrolysate (RSH). The bacterium was cultured in medium with an initial glucose concentration of 170g/L supplied as RSH. Once the glucose level had dropped to 20g/L, 60% of the culture volume was replaced with fresh medium contain...
Article
Full-text available
A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxy-canrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on the HPLC-UVD or LC-MS coupled with a ZORBAX Ecli...

Citations

... The basidiomycete Ustilago maydis causes corn smut disease; an Rho1 homolog of U. maydis is required for vegetative growth and is associated with cell polarity and cytokinesis, and Rho1 loss results in abnormalities in budding and chitin deposition [67]. In another basidiomycete, the edible mushroom Grifola frondosa, loss of Rho1 results in reduced mycelial growth, decreased amount of cell wall polysaccharides, and increased sensitivity to cell wall stress [68]. ...
... UDP-glucose can also be further converted into other glycosyl donors, such as UDP-Gal and UDP-GlcA [31,32]. In addition, fructose-6-phosphate can be converted into UDP-N-acetylglucosamine (UDP-GlcNAc) by UDP-N-acetylglucosamine pyrophosphorylase [33]. ...
... Research also shows the use of new and robust promoters ) and vector modification (Shi et al 2015) to overexpress the enzyme and enhance its level of 2-KGA (14) production. Genetic modifications of other species have also been developed to increase the production of 2-KGA (14) and may contribute with a framework for the improvement of G. oxydans strains (Zeng et al. 2019;Sun et al. 2020). ...
... With the aim of inhibiting the growth of fungal pathogens or screening targets for antifungal chemotherapies and fungicides, some knowledge of fungal glucan biosynthesis pathways has accumulated from many fungal species, including Saccharomyces cerevisiae, Aspergillus fumigatus, and Neurospora crassa ( Sánchez-León and Riquelme 2015; Vishukumar et al. 2017;Wang et al. 2018). On this basis, it has been determined that the deduced glucan synthesis pathway includes transport of glucose into cells as the substrate to form glucose-6-phosphate via catalysis by phosphoglucose isomerase (PGI), followed by synthesis of glucose-1-phosphate by phosphoglucomutase (PGM), synthesis of nucleoside diphosphate glucose (UDP-glucose) by UDP-glucose pyrophosphate phosphorylase (UGP), polymerization of UDP-glucose to glucan with a degree of polymerization up to ~ 1500 by glucan synthase complex near the plasma membrane (PM), and exudation to assemble the cell wall with chitin and mannoproteins (Cui et al. 2019;Cabibet and Arroyo 2013;Gow et al. 2017). Generally, glucan synthases can be classified as α-1,3-glucan synthases and β-1,3-glucan synthases based on their role in the synthesis of α-1,3-glucan and β-1,3-glucan, respectively. ...
... have all been shown to produce 2KGA (Hwangbo et al., 2003;Li et al., 2016). 2KGA-producing Pseudomonas species include P. corrugate, P. fluorescens, P. plecoglossicida, and P. aureofaciens (Trivedi and Sa, 2008;Sun et al., 2013;Umezawa et al., 2015;Wang et al., 2019). ...
... The picture in Figure 2B shows that the yield of polysaccharides increased with the extraction time (5-15 min) and decreased from 15 min. This was might be due to the fact that with the increase in extraction time, the polysaccharide is degraded due to the influence of the high temperature [40]. Therefore, the factor of time was selected at 10-20 min for the next experiment. ...
... While acetic acid can enter the Krebs cycle in the form of acetyl-CoA to be completely oxidized, propanoic acid needs more steps (Devlin 2004) and 1,2,3-Propanetricarboxylic 2-hydroxi acid was potentially generated through carboxylation as one of the intermediates of propanoic acid oxidation. 2-keto-Dgluconic acid abundant in DPs of PP is generally obtained by fermentation of glucose in microorgansims (Sonoyama et al. 1982;Chia et al. 2008;Wang et al. 2018). This metabolite can be readily converted to ascorbic acid (an antioxidant) chemically (Florent 1989), and possible equivalent mechanisms in plants cannot be discarded. ...
... The morphological analysis was based on previous literature [42], with minor modifications as follows. The where R L is the ratio of the loose layer in mycelial pellets, R max is the maximal mean radius of the mycelial pellets, and R core , is the radius of the dense core of mycelial pellets. ...
... These strategies are mainly based on physical and chemical methods, such as controlling the inoculum size, mechanical stress (aeration, stirring), pH, glass beads, surfactants, and other factors [14,15]. The maximum exopolymer yield of 2.33 g/L was achieved in Grifola frondosa when its morphology was controlled by changing the aeration rate and agitation [16]. The concentration of 2-mercaptohistidine trimethyl betaine increased approximately three times due to the change of morphological type in shiitake (Lentinula edodes) [17]. ...
... Moreover, strains of Gluconobacter oxydans (G. oxydans) [6], Pseudomonas [7], Serratia marcescens [8], Arthrobacter globiformis [9], Enterobacter intermedium [10], and Klebsiella pneumoniae [11,12] have also been used to produce 2-KGA. ...