Vl Singleton's scientific contributions

Citations

... The total phenolic content was determined using Folin-Ciocalteu reagent according to the method described by Singleton & Rossi (1965), with some modifications to adapt to 96 well microplate. Plant hydro-alcoholic extracts (25 µL) were mixed with 125 µL of Folin-Ciocalteu reagent (0.2 N) and 100 µL of 7.5% Na2CO3, and the absorbance was measured at 760 nm after 2 h of incubation at room temperature. ...