Vitor A. C. Pavinato's research while affiliated with The Ohio State University and other places

Publications (19)

Article
Mating has profound physiological and behavioral consequences for female insects. During copulation, female insects typically receive not only sperm, but a complex ejaculate containing hundreds of proteins and other molecules from male reproductive tissues, primarily the reproductive accessory glands. The post-mating phenotypes affected by male acc...
Preprint
Disentangling the effects of selection and drift is a long-standing problem in population genetics. Theoretical works based on simulations show that the signal of selection may bias demographic inference when it is pervasive. Ideally, interactions between selection and demography should be considered in the estimation of parameters of demographic a...
Preprint
Full-text available
The sequencing of whole or partial (e.g. reduced representation) genomes are commonly employed in molecular ecology and conservation genetics studies. However, due to sequencing costs, a trade-off between the number of samples and genome coverage can hinder research for non-model organisms. Furthermore, the processing of raw sequences requires fami...
Article
Human-mediated changes in landscapes can facilitate niche expansion and accelerate the adaptation of insect species. The interaction between the evolutionary history of the sugarcane borer, Diatraea saccharalis Fabricius, and historical and modern agricultural activity in Brazil shaped its spatial genetic structure, facilitating ecological divergen...
Article
Full-text available
The genus Helicoverpa (Lepidoptera: Noctuidae) includes phytophagous and polyphagous agricultural insect pests. In the Americas, a native pest, Helicoverpa zea (Boddie), and an invasive pest, Helicoverpa armigera (Hübner), are causing severe damage in vegetable and agronomic crops. The population structure of both species in South America is poorly...
Article
Aphids are emerging as model organisms for both basic and applied research. Of the 5000 estimated species, only three aphids have published whole genome sequences: the pea aphid Acyrthosiphon pisum, the Russian wheat aphid, Diuraphis noxia, and the green peach aphid, Myzus persicae. We present the whole genome sequence of a fourth aphid, the soybea...
Article
We present the development of a genomic library using RADseq (restriction site associated DNA sequencing) protocol for marker discovery that can be applied on evolutionary studies of the sugarcane borer Diatraea saccharalis, an important South American insect pest. A RADtag protocol combined with Illumina paired-end sequencing allowed de novo disco...
Conference Paper
In the current work, we are interested in studying population genomics of a phytophagous insect species Diatraea saccharalis, which is a hazardous pest for sugarcane and maize in Brazil. The species exhibits insecticide resistance and potentially presents host-associated interbreeding populations. We expect to access the consequences of geographica...
Article
Full-text available
We present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, Diatraea saccharalis (Fabricius). We isolated 16 microsatellite loci through an enriched genomic library protocol. After characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on...
Article
Substantial progress was made in the last decade in understanding the diversity in Dioscorea species. However, most of the studies so far concentrated in the ‘yam belt in Africa’. We present a genetic diversity and structure analysis among commercial and local varieties of water yam (Dioscorea alata L.) in Brazil using microsatellite and morphologi...
Article
We developed six microsatellite markers for the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). The SSR loci were isolated with enriched genomic library protocol by using native individuals as a genome source for markers. These loci were characterized in 48 individuals and they were tested for the ability to identify candidate migrant...
Article
Full-text available
The applicability of mitochondrial nad6 sequences to studies of DNA and population variability in Lepidoptera was tested in four species of economically important moths and one of wild butterflies. The genetic information so obtained was compared to that of cox1 sequences for two species of Lepidoptera. nad6 primers appropriately amplified all the...
Article
Full-text available
This article documents the addition of 229 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Acacia auriculiformis × Acacia mangium hybrid, Alabama argillacea, Anoplopoma fimbria, Aplochiton zebra, Brevicoryne brassicae, Bruguiera gymnorhiza, Bucorvus leadbeateri, Delphacodes dete...

Citations

... The functions of Sfps are largely unknown in the Mexican fruit fly, Anastrepha ludens [4,5]. This species is an important pest of mango and citrus, hindering commerce and food security [6]. ...
... Time-series studies do not require larger sample sizes than those employed in other population genomics studies of invasive species, (e.g., Kreiner et al., 2019;You et al., 2020) and are perfectly compatible with approaches such as Pool-Seq that can better facilitate large individual WGR sample sizes. By collecting and storing tissue soon after introduction, and at regular intervals over the course of invasive spread, WGR can be a powerful tool with which to determine the relative influence of pre-and post-introduction adaptation (e.g., Pavinato, De Mita, Marin, & de Navascués, 2021). Although RRS can also be informative when collected over a time-series, (e.g., Vandepitte et al., 2014) allele frequencies will go unmeasured at the vast majority of loci, potentially leading to an incomplete picture of the adaptive and demographic history of the invasion. ...
... Despite its benefits, GT-seq is not yet widely used outside of salmonids. Early applications to non-model organisms, however, have shown great promise for this method's versatility, including the ability to reveal dispersal and mating patterns in a complex environment (Baetscher et al., 2019), provide insight to the ecological and evolutionary dynamics of secondary contact (Reid et al., 2020), and understand population diversity in systems that are heavily influenced by climate change (Pavinato et al., 2019). Pedigree analysis in wild populations relies particularly heavily on the ability to genotype large sample sizes to increase the likelihood of detecting kin relationships, toward which GT-seq is ideally suited. ...
... The high genetic variation and a wide plant host range have also supported the success of this pest in colonizing regions with different environmental conditions, and human migration may likely be a major factor that contributed to its spread throughout the Americas (Francischini et al., 2019). Furthermore, the great increase of genetic diversity of the sugarcane borer in Brazil coincides with the expansion of agricultural production of sugarcane and maize crops in the Brazilian landscape (Pavinato et al., 2018). This makes D. saccharalis an interesting organism to study a wide range of questions including ecological adaptation and evolutionary dynamics. ...
... To determine the specific identity of the parents, two pairs of H. armigera and two of H. zea were identified by genital morphology, 28 complemented by microsatellite amplification using primers designed for each species. 29,30 2.1.1 Adult stage Moths were maintained in cylindrical plastic cages (15 cm in diameter and 15 cm high). The cages were closed at the top with brown voile and at the bottom with polypropylene Petri dishes. ...
... As one of the main pests of soybean, soybean aphids have drawn attentions of researchers all over the world. Several researches have focused on aphid resistant soybean germplasm screening (Fox et al. 2014;Natukunda et al. 2019), mapping of aphid resistance genes (Hanson et al. 2018), classification of aphid biotypes (Todd et al. 2016;Bhusal et al. 2021), analysis of the aphid genome (Giordano et al. 2020;Wenger et al., 2020), and molecular mechanism of soybean resistance to aphids Yates-stewart et al. 2020). However, the researches on soybean aphids in China were relatively laggard and most of them were related to the perspective of insects and plant protection, including the biological characteristics and population distribution of aphids, the yield and quality loss caused by soybean aphids, and the control of aphids. ...
... Compared with other molecular markers, SNPs are widely distributed in genomic DNA sequences (Edwards et al. 2007). Furthermore, SNPs show high polymorphism, genetic stability, and accuracy and resolution (Pavinato et al. 2017;Wang et al. 2017;Zhou et al. 2015). With the development of high-throughput sequencing technology, SNP markers have been widely used in grape genetic linkage map construction (Barba et al. 2014;Guo et al. 2015b;Hyma et al. 2015;Sapkota et al. 2019;Smith et al. 2018;Teh et al. 2017;Tello et al. 2019;Wang et al. 2017;Zhu et al. 2018). ...
... Breeding programs of Chinese yam mainly focused on direct selection within species . Thus, in addition to the possibility of similar environments, it was in all probability the frequent exchange of germplasms between farmers in the nearby areas (Mwirigi et al. 2009;Siqueira et al. 2014). It is worth noting that there was a close Euclidean distance between Anyuan L No.2 and Yudu L No.2, Ruijin L and Shanggao L, Yudu L No.2 and Zhouning L ( Fig. 3; Table S5), which may be resulted from the confusion of various records and nomenclatures in neighboring areas. ...
... Microsatellite genotyping. The microsatellite loci used in the study were provided by the Laboratory of Conservation Genetics and Genomics, Agribusiness Technological Development of São Paulo, Brazil and were developed by Pavinato [57]. Details and characteristics of the microsatellites are shown in S1 Table. ...
... Microsatellites have previously been identified in FAW using populations from Texas, Mississippi, Puerto Rico and Brazil 17,18 . These microsatellites were variable enough to distinguish between three genetically distinct populations and were able to identify migrants between those populations 18 . ...