August 2024
·
2 Reads
This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.
August 2024
·
2 Reads
August 2024
·
5 Reads
August 2024
·
3 Reads
December 2023
·
78 Reads
·
1 Citation
Bergenia ciliata (Haw.) Sternb. is an important herb predominantly found in the Indian Himalayan Region. It is widely used in medicines, healthcare systems, cosmetics, fodder, and ornamental purposes. The Illumina sequencing and de novo transcriptome assembly were carried out in B. ciliata to develop and identify simple sequence repeat markers. A total of 18 226 simple sequence repeats (SSRs) were identified wherein di-nucleotides were found to be abundant (47.88%), followed by mono-nucleotide (35.03%) and tri-nucleotide (15.88%) repeats. A total of 11 839 EST-SSR primers were designed, of which 96 primer pairs were commercially synthesized. Finally, 17 primer pairs revealed clear, distinct polymorphic bands, and these primers were validated with 40 diverse B. ciliata accessions. The present study revealed moderate level of genetic diversity (Ho = 0.389, He = 0.542, and PIC = 0.513). Furthermore, the transcriptome data and EST-SSR markers generated during the present investigation could be an important genetic resource for functional genomics, population studies, and conservation genetics of the genus Bergenia.
September 2023
·
49 Reads
August 2023
·
90 Reads
·
1 Citation
Nucleus (India)
Abstract Termitomyces are found associated with termites and show an exo-symbiotic relationship with termites. Both termites and Termitomyces are important in forest ecosystems for wood degradation. The morphological diversity of Termitomyces mushrooms as observed in Assam is high similar to other forest-rich regions of India. But the genetic diversity and species diversity from the region is not yet documented well. In the present study, DAMD and ISSR markers were applied for genetic diversity estimation and nrDNA ITS marker was applied for the phylogenetic study of the Termitomyces mushrooms collected from diferent localities of Assam. Besides, morphological characters were compared to the existing species monographs to identify the species. Based on morpho-molecular studies, the species identifed are T. heimii, T. microcarpus, and T. eurrhizus. Five morphotypes of Termitomyces diferent from the existing species descriptions are recorded and are also sub-clustered distinctly and distantly in a phylogenetic tree generated based on nrDNA ITS sequences. The presence of novel species of Termitomyces is very much likely in the region which needs further taxonomic engagements to establish the neo species through valid publication.
October 2022
·
147 Reads
Bergenia ciliata (Haw.) Sternb. is an important herb predominantly found in Indian Himalayan Region (IHR). It is widely used in medicines and healthcare system, cosmetics, food, fodder, and ornamental purposes. Illumina sequencing and de novo transcriptome assembly were carried out in B. ciliata to develop and identify simple sequence repeat markers for genetic diversity and conservation studies. The assembled data generated a total of 65,010 unigenes that showed significant similarities when compared with seven functional databases including 53,577 (Non-Redundant Protein Sequence Database: 82.41%), 44,297 (Nucleotide Sequence Database: 68.14%), 42,287 (Swiss Prot: 65.05%), 15,027 (Eukaryotic Orthologous Groups: 23.11%), 22,540 (KEGG Orthology: 34.67%), 29,477 (Gene Ontology: 45.34%) and 20,609 (Pfam: 31.7%) unigenes. In this study, a total of 18,226 SSRs and 14,497 SSR containing sequences were identified. Dinucleotides were found to be abundant (47.88%) in B. ciliata followed by mononucleotides (35.04%), and trinucleotides repeat (15.90%). AG/CT was the most common di-nucleotide repeat (40.33%). A total of 11,839 EST-SSR primers were designed, of which 96 primer pairs were synthesized randomly. Finally, 18 primer pairs were selected that revealed clear, distinct polymorphic bands when examined in eight diverse B. ciliata accessions. Furthermore, the transcriptome data and the EST-SSR markers will be an important resource for investigating genetic diversity in B. ciliata and other species of the family Saxifragaceae.
... 32 On the other, Rana et al. observed 0.513 value of PIC which was higher than observed by us in current study. 33 A high level of genetic diversity was reported in another Himalayan herb Rheum australefrom western Himalayan region. 34 Pant et al. also recorded 0.46 value of PIC which was almost equal to the value detected by us herein. ...
December 2023