Vaihinger HM's research while affiliated with Ruhr-Universität Bochum and other places

Publications (43)

Book
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Kreißsaalführungsseminar B-O-C-H-U-M: B - BEL-Entbindung (A.Feige) O - Organisation und Dokumentation im Kreißsaal (W. Müller, M. Kumbartski) C - CTG-Kurs und Risikomanagement (R. Berger, J. Middelanis) H - Hands on am Phantom: Glocke-ZangeSchulterdystokie (K. Marschner, B. Holmer, B. Karbowski) U - Untersuchung und Reanimation des Neugebornen (N...
Conference Paper
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Im Spannungsfeld zwischen Qualität und zunehmendem ökonomischem Druck/nötiger Kostenbegrenzung müssen und können Behandlungsprozesse in hohem Maße standardisiert werden. Immer knapper werdende Ressourcen im Personal- und Sachkostenbereich müssen durch Vermeidung redundanter Leistungen, fehlender Absprachen oder von Zeitverlusten durch divergierende...
Conference Paper
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Introduction: The long term aim of this project is the utilisation of human umbilical cord blood (hUCB) stem cells in the regeneration of (perinatal) brain damage and peripheral nerve injury. A major prerequisite for the development of hUCB based cell replacement therapies is the in vitro proliferation and differentiation of neural stem cells. Met...
Conference Paper
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Hypoxic-ischemic cerebral damage is an important contributor to perinatal mortality and morbidity. Experimental evidence has shown that neuroprotective strategies using pharmacologic agents can alleviate perinatal brain damage. However, so far no convincing strategies are available for the regeneration of damaged structures of the perinatal nervous...
Article
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We tested the neuroprotective effects of creatine against hypoxic-ischemic injury in the immature brain. Hippocampal slices were prepared from fetal guinea pigs at 0.9 gestation and incubated in artificial cerebrospinal fluid (aCSF) equilibrated with carbogen. Slices were subjected to oxygen-glucose deprivation (OGD) for 30 or 40 minutes. Two hours...
Article
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Per year, approximately 1,000 children in Germany suffer from brain damage due to hypoxic-ischemic insults during the perinatal period. Based on the severity and localization of the insult, these children develop either spastic pareses, choreoathetosis, ataxia, or sensomotoric dysfunctions. A close cooperation between obstetricians, pediatricians,...
Presentation
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Präsentation auf dem Wissenschaftlichen Symposium ”Adulte Stammzellen” -- Perspektiven für die Therapie am 28. Juni 2003 in Mönchengladbach. Gastgeber Prof. Dr. Hans E. Reis und Dr. med. G. Döhmen, Mönchengladbach. Die Beiträge wurden in der Zeitschrift ’Medizinische Klinik’, Verlag Urbahn & Vogel, Jg. 94, Suppl. II, S. 1-45, am 15. Dezember 2003 p...
Article
Perinatal brain damage is associated not only with hypoxic-ischemic insults but also with intrauterine inflammation. A combination of antenatal inflammation and asphyxia increases the risk of cerebral palsy >70 times. The aim of the present study was to determine the effect of intracisternal (i.c.) administration of endotoxin [lipopolysaccharides (...
Article
Full-text available
Perinatal brain damage is associated not only with hypoxic-ischemic insults but also with intrauterine inflammation. A combination of antenatal inflammation and asphyxia increases the risk of cerebral palsy >70 times. The aim of the present study was to determine the effect of intracisternal (i.c.) administration of endotoxin [lipopolysaccharides (...
Poster
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A MODEL OF NEONATAL CEREBRAL ISCHEMIA IN RATS (‘LEVINE’) TO STUDY NEUROPROTECTION AND NEUROREGENERATION J. MIDDELANIS1, H.-M. VAIHINGER1; Y. GARNIER1, K. GOTTMANN2, H. DINSE3, A. JENSEN1 1 Department of Obstetrics & Gynecology, University of Bochum 2 Institute of Cell Physiology, University of Bochum 3 Department of Neuroinformatics, University of...
Poster
Full-text available
CEREBRAL ISCHEMIA AND UMBILICAL STEM CELL TRANSPLANTATION IN CHRONICALLY PREPARED FETAL SHEEP Y. GARNIER1, H.-M. VAIHINGER1, J. MIDDELANIS1, O. BRÜSTLE2, A. JENSEN1 1Department of Obstetrics & Gynecology, University of Bochum 2Institute of Reconstructive Neurobiology, University of Bonn Objective: Hypoxic-ischemic cerebral damage is an important co...
Data
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First International Meeting of the Stem Cell Network North Rhine Westphalia, Düsseldorf, November 26-27, 2002; 11/2002
Data
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First International Meeting of the Stem Cell Network North Rhine Westphalia, Düsseldorf, November 26-27, 2002; 11/2002
Presentation
Full-text available
Präsentation Klausurtagung Kompetenznetzwerk Stammzellforschung NRW

Citations

... Spastic Paresis. First mention of the concept of amelioration of perinatal brain damage by neuroregeneration using cord blood dates back to 2002, when our preliminary data in chronically prepared fetal sheep showed a highly specific invasion of human umbilical cord blood mononuclear cells (huMNC) into the damaged brain region, that is, "homing, " in a model of cerebral ischemia [13]. This was the beginning of a series of experiments in vitro and in vivo, using a different animal model ("Levine") of perinatal cerebral hypoxic-ischemia in newborn rats to increase effectiveness [14,15]. ...
... First mention of the concept of amelioration of perinatal brain damage by neuroregeneration using cord blood dates back to 2002, when our preliminary data in chronically prepared fetal sheep showed a highly specific invasion of human umbilical cord blood mononuclear cells (huMNC) into the damaged brain region, that is, "homing, " in a model of cerebral ischemia [13]. This was the beginning of a series of experiments in vitro and in vivo, using a different animal model ("Levine") of perinatal cerebral hypoxic-ischemia in newborn rats to increase effectiveness [14,15]. The "Levine" model includes unilateral carotid artery ligation followed by 8% O 2 inhalation for 80 minutes on postnatal day (PN) 7. The neurodevelopment of the rat at PN 7 is equivalent to that of the human brain at birth [16]. ...
... In the past ten years, the combination of infection/inflammation plus hypoxia/ischemia (HI) has been reproduced experimentally in rats at early neurodevelopment stages in rat pups between P1 and P7 equivalent to early and late preterm development of human neonates [10][11][12][13][14]. Lipopolysaccharide (LPS) is an important component of Gram-negative bacteria and has been used to make animal models of inflammation. ...
... When LPS was injected mid-or full gestation into pregnant sheep, there were observable injuries to the fetal brain, particularly in the white matter [125][126][127][128]. Intracerebral LPS exposure showed increased pro-inflammatory markers and caused a loss of oligodendroglial precursors. Intracerebral LPS exposure was also implicated in loss of myelination and bilateral ventricular dilation [129][130][131]. In addition, Oskvig et al. showed that pregnant Sprague Dawley rats exposed or challenged with LPS showed an increase in maternal serum cytokines [132]. ...
... Eight out of 17 studies were performed in perinatal models of HIE (Table 2). Six out of 8 studies were conducted in rodents between postnatal days (P)7 and P20 [18][19][20][21][22][23][24], which is considered comparable to human brain development between near-term/term and late infancy [25,26]. One study was conducted in term spiny mice which in terms of brain development are broadly comparable to the term human [27]. ...
... As we have shown previously in an acute perinatal cerebral hypoxic-ischemia model in newborn rats [8,9], mononuclear cells derived from human cord blood show a highly specific "homing" and migrate into the lesioned region of the brain in large numbers within 24 hours when given intraperitoneally [9,10]. This chemotactic process is mediated in part by stromal-derived factor-(SDF-) 1, a chemokine that is expressed in the lesioned brain, and transplanted human umbilical cord blood cells expressing the SDF-1 receptor CXCR4 migrate to the lesioned site [11]. ...