Vadim Ivanov's research while affiliated with Dr. Rath Health Foundation and other places

Publications (55)

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Despite vaccine availability, the global spread of COVID-19 continues, largely facilitated by emerging SARS-CoV-2 mutations. Our earlier research documented that a specific combination of plant-derived compounds can inhibit SARS-CoV-2 binding to its ACE2 receptor and controlling key cellular mechanisms of viral infectivity. In this study, we evalua...
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SARS-CoV-2 pandemic remains a challenge to human health and economy worldwide. Previously we have shown that a combination of active plant-derived compounds and plant extracts can dose-dependently inhibit binding of RBD-spike protein SARS-CoV-2 to the ACE2 receptor and its expression on human alveolar epithelial cells. Here we use eGFP-luciferase-S...
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Background Angiotensin-converting enzyme II or ACE2 is an integral membrane protein present on many types of cells, including vascular endothelial cells and lung alveolar epithelial cells. This receptor serves as the entry point for SARS-coronaviruses (SARS-CoVs), including a novel coronavirus 2019-nCoV. Limited availability of these receptors can...
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Vascular calcification is a pathophysiological process that is associated with coronary atherosclerosis, and is a prognostic marker of cardiovascular morbidity and mortality. The process of arterial wall calcification is triggered and accompanied by pro-osteogenic phenotypical modifications of resident smooth muscle cells (SMC). Vitamin C (ascorbic...
Article
Calcium, sodium and potassium channel blockers are widely prescribed medications for a variety of health problems, most frequently for cardiac arrhythmias, hypertension, angina pectoris and other disorders. However, chronic application of channel blockers is associated with numerous side effects, including worsening cardiac pathology. For example,...
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Degradation of the extracellular matrix (ECM) plays a critical role in the formation of tumors and metastasis and has been found to correlate with the aggressiveness of tumor growth and invasiveness of cancer. Ascorbic acid, which is known to be essential for the structural integrity of the intercellular matrix, is not produced by humans and must b...
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Periodic outbreaks of African swine fever virus (ASFV) infection around the world threaten local populations of domestic pigs with lethal disease and provide grounds for pandemic spread. Effective vaccination may bring this threat under control. We investigated the effectiveness of select peptides mimicking viral proteins in establishing a protecti...
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The dysregulation of renin-angiotensin system-signaling is a contributing factor to the development of atherosclerosis and cardiovascular disease. We studied the progression of angiotensin II (AngII)-induced arterial wall atherosclerosis after its induction. Atherosclerosis was accelerated in ApoE (-/-) C57B6 mice by 4 weeks of continuous osmotic p...
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We studied the effect of nutrient/herbal extract mixture (NM) supplementation prior to angiotensin II (AngII) administration on ApoE KO mice. AngII-mediated injuries, which included increased atherosclerotic lesion size (by 31%) and severity (by 200%), as well as an increased number of necrotic cores (by 44%), were attenuated by NM supplementation,...
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Mammary tumors were developed by intraperitoneal injection of N-methyl-N-nitrosourea (MNU) in 21-day-old, sexually immature female Wistar rats. Injection of MNU was repeated 14 weeks after the first one. When palpable tumors were evident in all of the rats, various dietary treatments were initiated for a period of 8 weeks. The treatments were desig...
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Epigallocatechin gallate (EGCG), a main anticancer component in green tea, has a poor bioavailability in rats and humans due to oxidation, metabolism and its efflux. It was hypothesized that nutrients that address these problems might result in increased bioavailability. Plasma concentrations of EGCG at various time intervals were determined to cal...
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Highly metastatic melanoma is resistant to existing therapies. Our main objective was to investigate the effect of a nutrient mixture (NM) on B16FO tumor growth and hepatic metastasis. Tumor growth was studied in athymic nude male mice, 5-6 weeks old, inoculated with 10(6) B16FO melanoma cells subcutaneously and fed either a regular diet or one sup...
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Monocyte adhesion to endothelium plays an important role in atherosclerosis. We investigated the effects of micronutrients on monocyte-binding properties of extracellular matrix (ECM) produced by human aortic endothelial cells (AoEC). Confluent cultures of AoEC were exposed to ascorbic acid, quercetin, gotu kola extract (10% asiatic acid), green te...
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Numerous outbreaks of avian influenza virus infection (A/H5N1) have occurred recently, infecting domestic birds, chicken and ducks. The possibility of the emergence of a new strain of influenza virus capable of causing a pandemic in humans is high and no vaccine effective against such a strain currently exists. A unique nutrient mixture (NM), conta...
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Rhabdomyosarcoma, the most common pediatric soft tissue sarcoma of mesenchymal origin, has metastasized in ∼25% of all patients at time of diagnosis. Though current treatment strategies have achieved some success, they are associated with severe adverse effects. We investigated the effect of a nutrient mixture (NM), which has shown antitumor effect...
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Extracellular matrix (ECM) function and structure are severely compromised at atherosclerotic lesion sites, contributing to initiation and progression of the disease. This study investigated whether ECM biological properties would be beneficially affected by exposure to nutrients essential for collagen synthesis and posttranslational modification....
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Standard multimodality therapy of gliomas is associated with poor patient survival and significant toxicity. Abnormal expression of matrix metalloproteinases is associated with tumor growth and invasion. Based on reported antitumor properties, we investigated the effect of a combination of natural compounds (NM), primarily composed of lysine, proli...
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Certain drastic behavioral modifications by arterial wall smooth muscle cells (SMC) have been considered key steps in the formation of atherosclerotic lesions: massive migration of SMC from the media to the intima layer of the vessel, dedifferentiation of SMC to proliferating phenotype, and increased secretion of inflammatory cytokines as a respons...
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Current treatment of testicular cancer is associated with secondary malignancy, infertility, and cytotoxicity. Based on reported antimetastatic potential, we investigated the effect of a nutrient mixture (NM) containing lysine, proline, arginine, ascorbic acid, and green tea extract on human testis cancer cell line NT 2/DT by measuring cell prolife...
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Five-year survival is limited to 60% in renal cancer patients at diagnosis. Due to the cancer's resistance to conventional treatments and associated high morbidity, we investigated the antimetastatic effects of a specific nutrient mixture (NM) containing lysine, proline, arginine, ascorbic acid and green tea extract on human renal adenocarcinoma ce...
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The authors investigated the effect of a nutrient mixture (NM) on lung metastasis by B16F0 melanoma cells in C57BL/6 female mice. Mice were divided into equal groups (1 to 6) and injected via tail vein with B16F0 cells (groups 1 to 4), B16FO cells pretreated with NM (group 5), or saline (group 6). Groups 1, 3, 4, 5, and 6 were fed the control diet...
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The high incidence of lung cancer and ineffective toxic action of current mono and doublet chemotherapy approaches result in poor patient survival. Further, matrix metalloproteinases (MMPs) are implicated in neoplastic invasion and metastasis. Based on this, the authors investigated the effect of a dietary micronutrient mixture (NM) containing lysi...
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Malignant mesothelioma (MM), an asbestos-associated cancer with no known cure, is a highly aggressive tumor causing profound morbidity and nearly universal mortality. Extracellular matrix (ECM) matrix metalloproteinases (MMPs) produced by tumor and stromal cells play a key role in tumor invasion and metastasis. Prevention of ECM degradation by MMP...
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Based on the poor prognosis associated with ovarian cancer and reported anticancer properties of specific nutrients, we investigated the effect of a nutrient mixture (NM) containing lysine, proline, arginine, ascorbic acid and epigallocatechin gallate on human ovarian cancer cells SK-OV-3 by measuring: cell proliferation, modulation of matrix metal...
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Bladder cancer, the fourth highest incident cancer in men and tenth in women, is associated with a high rate of recurrence, even when treated in situ, and prognosis is poor once the cancer metastasizes to distant sites. Based on anticancer properties, we investigated the effect of a mixture of lysine, proline, arginine, ascorbic acid, and green tea...
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Abnormal uterine myometrial contractility causes preterm delivery, contributing to perinatal morbidity and mortality. Disturbances in hormonal regulation and inflammation-related processes have been attributed a role in the pathophysiological mechanisms of uterine contractility. We investigated the effects of natural nutrients on uterine tissue con...
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Structural changes in the extracellular matrix (ECM) are necessary for cell migration during tissue remodeling. MMPs, VEGF, Ki-67 (proliferative protein), and constituents of ECM play a critical role in angiogenesis and underlie neoplastic invasion and metastasis. This prompted us to investigate the effect of a diet containing lysine, proline, argi...
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Current treatment of fibrosarcoma, an aggressive cancer of the connective tissue, is generally associated with poor prognosis. Matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and constituents of the extracellular matrix (ECM), such as fibronectin, play a critical role in angiogenesis and underlie neoplastic invasion and...
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A hallmark of renal cell carcinoma (RCC) invasion is its ability to degrade ECM by local production of gelatinase enzymes. Although many studies on RCC have demonstrated the importance of MMPs, very little information is currently known regarding the effect of inducers and inhibitors. We therefore investigated the effect of inducers and inhibitors...
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Matrix metalloproteinases (MMPs) have been recognized as key players in the degradation of the extracellular matrix (ECM) by migration and proliferation of endothelial cells and their subsequent invasion of the underlying stroma. The prevention of ECM degradation through the inhibition of MMP activity has been shown to be a promising therapeutic ap...
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Melanoma, a very serious form of skin cancer, causes the most skin cancer-related deaths, due to metastasis. Structural changes in the extracellular matrix (ECM) are necessary for cell migration during tissue remodeling. MMPs, VEGF, Ki-67 (proliferative protein) and constituents of ECM play a critical role in angiogenesis, and are crucial in neopla...
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Degradation of extracellular matrix (ECM) is a hallmark of tumor invasion, metastasis and angiogenesis. Based on the Rath multitargeted approach to cancer using natural substances to control ECM stability and enhancing its strength, we developed a novel formulation (NM) of lysine, proline, ascorbic acid and green tea extract that has shown signific...
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Colorectal cancer is the second most deadly cancer in the United States. When diagnosed early, current treatments bring a limited success; however, once metastasis occurs, radiation and chemotherapy are generally ineffective. Structural changes in the ECM are necessary for cell migration during tissue remodeling. Matrix metalloproteinases (MMPs), V...
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Current treatment of osteosarcoma is associated with poor prognosis, especially due to the increased risk of developing other cancers with chemotherapy. Therefore, new, safe and effective treatment strategies are needed. We investigated the effect of a unique mixture of nutrients containing lysine, proline, arginine, ascorbic acid, and epigallocate...
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The limited ability of current treatments to control metastasis and the proposed antitumor properties of specific nutrients prompted us to examine the effect of a specific formulation (nutrient supplement [NS]) of lysine, proline, arginine, ascorbic acid, and green tea extract in vivo on the development of N-methyl-N-nitrosourea (MNU)-induced mamma...
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Current treatment of pancreatic cancer is generally associated with poor prognosis, even if diagnosed early, owing to its aggressive rate of metastasis and non-responsiveness to chemotherapy and radiotherapy. Matrix metalloproteinases (MMPs) have received much attention in recent years for their role in various malignancies, and have been implicate...
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Current treatments are generally ineffective once breast cancer has metastasized; median survival is reduced to 2-3 yr. Previous research studies demonstrating potent synergistic antitumor activity of lysine, proline, ascorbic acid, and epigallocatechin gallate prompted us to investigate the in vivo inhibitory effect of a nutrient mixture containin...
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Matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), Ki 67 (proliferative protein) and constituents of ECM play a critical role in angiogenesis, and are crucial in neoplastic invasion and metastasis. Based on the antitumor properties of certain nutrients, we investigated the effect of a diet containing lysine, proline, argin...
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Once prostate cancer has metastasized, current treatment methods are generally ineffective. Due to the reported anti-tumor properties of specific nutrients, we investigated the effect of a unique formulation (NS) of lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on human prostate cancer cell lines: PC-3, DU145 (androgen inse...
Article
Current treatment of osteosarcoma is associated with poor prognosis, especially due to the increased risk of developing other cancers with chemotherapy. Therefore, new safe effective treatment strategies are needed. This prompted us to investigate the synergistic effect of a nutrient mixture (NS) of lysine, proline, arginine, ascorbic acid, and epi...
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Introduction: Matrix metalloproteinases (MMPs) have received much attention in recent years for their role in various malignancies, and have been implicated in tumor invasion, metastasis and angiogenesis. Certain MMPs, such as MMP-2 and MMP-9, have shown a special propensity for promoting cancer. Development of MMP inhibitors has been a recent appr...
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Limitation of current treatment methodologies to con- trol metastasis, as well as the proposed antitumor properties of specific nutrients, prompted us to investigate the effect of a specific formulation (NS) of lysine, proline, arginine, ascorbic acid, and epigallocatechin gallate on human colon cancer cells HCT 116 for viability, MMP expression, i...

Citations

... According to Kumar et al. [18], magnesium ascorbate has been revealed as, among 106 other nutraceticals, the most powerful inhibitor of M PRO /3CL PRO , a key protease in SARS-CoV-2, that as a nonstructural protein participates in the replication and life cycle of the virus inside the host cell. Due to its interaction with ACE2, vitamin C is also mentioned as one of the natural compounds having the inhibitory activity against different variants of coronavirus, such as Alpha-, Beta-, Gamma-, Delta-, Kappa-or Mucoronavirus [19]. As was shown by Ivanov et al. [20], AA and its specific combinations with other natural compounds lower ACE2 expression at the protein level and at the RNA level as was proved in Human Small Airways Epithelial Cells (SAEC) and Human Microvascular Endothelial Cells (HMEC). ...
... Since the emergence of COVID-19, several studies have shown the efficacy of such compounds as inhibitors [14][15][16][17]. Our earlier studies also identified the efficacy of plant-derived compounds on several cellular mechanisms of SARS-CoV-2 infectivity [18][19][20]. In this study, we evaluated the efficacy of a specific combination of micronutrients and plant extracts against newly emerged mutated forms of SARS-CoV-2 and the activity of key enzymes involved in viral processing in human alveolar cells. ...
... Clinically, the correlation between adverse outcomes of respiratory infections and hypertension was indicated and attributed to increased expression of Angiotensinconverting enzyme II (ACE2) [60]. Recently, it has been shown that ascorbic acid administration can play controlling role on cellular expression of ACE2 [61]. On the other hand, administration of low-dose hydrocortisone is recognized as the strongest activator of ACE2 [62]. ...
... Biochemical markers of dysfunction of endothelial alongside inflammation are cellular adhesion molecules. The activity of oxidized-low density lipoprotein (oxLDL) that is responsible for the stimulation of inflammation in the endothelium cells, and causes atherosclerosis can be neutralized by supplementation of vitamin C [77]. Markedly, some researchers suggested that in diabetic patients, administration of vitamin C upgraded the endothelial role. ...
... In this function, vascular deposits could prevent the deadly consequences of blood loss from scurvy [4]. However, with sustained long-term vitamin C deficiency, various pathological conditions develop, leading to atherosclerosis, further aggravated by the prothrombotic and proinflammatory effects of Lp(a) [5,6]. ...
... It acts as a co-factor for the proline and lysine hydroxylases that stabilize the collagen molecule tertiary structure, and it also promotes collagen gene expression. 13 Two previous studies revealed excellent improvement in acne scars with topical application of vitamin c preceded by microneedling. 14,15 The role of topical insulin in wound healing has been under scrutiny in literature since 1960's and 1970's. ...
... Several studies of proline and leucine-rich peptides have demonstrated a variety of biological activities Dolashka Fig. 8 Schematic diagram for anti-MMP-9 action of the peptide from P. lutheri in activated HT1080 fibrosarcoma cells Arai et al. 2012). Furthermore, MMP-9 activity has been shown to be reduced in the presence of proline and leucine-rich peptides (Roomi et al. 2004;Nguyen et al. 2013). Our results support these previous findings, as the proline and leucine-rich peptide we isolated from P. lutheri also inhibits MMP-9 expression. ...
... The effect of NM on apoptosis in rhabdosarcoma cells is shown inFig. 12 [40]. At 100 μg/ml NM, 95% of cells were viable and 5% in apoptosis, at 250 μg/ml NM, 46% were viable, 13% in early apoptosis and 41% in late apoptosis, at 500 μg/ml NM, 28% of cells were viable, 14% in early apoptosis, and 58% in late apoptosis. ...
... We found 5-aminovalerate to be significantly enriched within the arginine and proline metabolism (p-value: 0.00243) and D-arginine and D-ornithine metabolism (p value: 0.0541) pathways. Lysine, proline and arginine have previously shown antitumour effects on cancer cell line HCT 116 by inhibiting MMP expression and invasion [65]. Although it may be thought that their metabolism would be downregulated in cancer if a tumour has been able to develop, a systematic review evaluating the effects of arginine on colorectal cancer found decreased tumour production and crypt cell hyperproliferation during the initiation stage of carcinogenesis, but a stimulation of tumour growth during the promotion stage [66]. ...
... For the hematologic tumors and some solid tumors, such as melanoma, VitC inhibits tumor growth and even reverses malignant transformation through epigenetic regulation of gene expression profiles [11,12]. Melanoma and breast cancer in VitC-deficient mice exhibited a higher propensity for invasiveness and metastasis, while restored VitC inhibited those malignant behaviors [13]. In ovarian cancer, the combination of VitC with carboplatin and paclitaxel suppresses xenograft growth and possesses considerably stronger efficiency than chemotherapy alone. ...