V.G. Rebrov’s research while affiliated with Saratov State Medical University and other places

Materials and methods: water solution of Blemaren medication with pH=6,15 in concentrations corresponding to single portion of medication (1 tablet m=3,5282 g per 250 ml of water) and uroliths (oxalates, urates including uncommon xanthine calculi). Composition of calculi was determined by means of X-ray tests and IR spectroscopy. Photometry of Blemaren with saluted portion of calculi was conducted. Results: it was established that in the Blemaren solutions which had been previously exposed to 2 Hz alternating magnetic filed during one hour the solution process is 1.92 - 2 times more effective than in common water solutions. Discussion - in control solutions pH values increased 5.65 - 6.8 times in the course of time, whereas in the Blemaren solutions exposed to alternating magnetic field pH values remained virtually unaltered during the whole experiment. Conclusion: there were detected significant differences in pH values of Blemaren solutions and its solvent properties between solutions which were exposed to alternating magnetic filed and those which were not.

A 27% increase in solubility of urolith substance was observed in water pretreated with alternating magnetic field (22 Hz, 25 mT) for 60 min compared to untreated water. Changed density of distilled water without concrement in response to the impact of alternating magnetic field does not influence qualitatively on solubility of urolith substance in the urolith placement in the pretreated water. Shift in wave length of maximums in spectra of solution absorption evidences for more intensive solution of organic components of uroliths in water pretreated with alternating magnetic field than in untreated water.

In recent years it has been discovered that a long-term exposure to low frequency magnetic fields leads to changes in activity of biological systems both in vivo and in vitro. Molecular mechanisms of this phenomenon are not clear. The present work uses infrared (IR) spectroscopy to study the effect of alternating magnetic field on a structural state of purified proteins. It was revealed that a 1-h exposure of aqueous solution of bovine serum albumin (BSA) and gluten isolated from wheat to 5.75Hz magnetic field with maximum amplitude of 25mTl resulted, respectively, in a ∼1.5- and 2-fold increase of the width of the band related to the vibrations of valent bonds in the range of 3500-2750cm(-1) (p<0.05). Unlike aqueous solutions, the desiccated BSA films did not exhibit any effect of magnetic field on parameters of IR-spectra. It is suggested, that low frequency magnetic fields induce the broadening of bands in IR spectra due to changes in structural organization of delocalized protein-bound water molecules thereby affecting macromolecules and related cell reactions.