V L Ponosov’s scientific contributions

CBA, CC57BR, C57B1/6, BALB/c, and outbred white mice were intraperitoneally or subcutaneously (C57B1/6 strain) immunized with sheep red cells in a dose optimal for the development of delayed-type hypersensitivity but subthreshold for antibody production. Seven days later the mice were reimmunized with sheep red cells in various doses subcutaneously (CBA, C57B1/6, BALB/c, outbred mice) or intraperitoneally (CBA, CC57BR, outbred mice), and 5 days after reimmunization the intensity of antibody production and delayed-type hypersensitivity was assessed. Intact mice were controls. The immunization was found to selectively enhance delayed-type hypersensitivity in C57B1/6, CC57BR, and BALB/c mice and to intensify antibody production in CBA mice; both phenomena were observed in outbred mice.

The manifestation of a secondary immune response to intraperitoneal and subcutaneous injection of sheep red cells in various doses was studied in CBA and C57Bl/6 mice. The parameters under study were the delayed-type hypersensitivity reaction and antibody production assessed from the levels of antibody-producing cells of classes M and G in the lymph node and spleen. The manifestation and correlations between delayed-type hypersensitivity and antibody production were found to depend on the route of antigen administration and its first immunizing dose, interval between the two immunizations, and genetic control of the immune response.

In experiments made on noninbred white mice the manifestations of delayed hypersensitivity and the number of antibody-producing cells in regional lymph nodes or the spleen were determined on day 5 after the subcutaneous or intraperitoneal immunization of the animals with sheep red blood cells (SRBC). At the same time the injection of S. aureus antigens simultaneously with SRBC raises the threshold of antigenic sensitivity and decreases the manifestations of cell-mediated and humoral immune response to SRBC. This suppressive effect was even more pronounced in the local immune process and was partially mediated by the hyperproduction of prostaglandins.

In experiments on CBA and C57BL/6 mice the generation of antibody-forming cells respectively either in the popliteal lymph nodes or spleen as well as a rate of delayed type hypersensitivity response (DTHR) on the background of subcutaneous (into foot) or intraperitoneal injection of different doses of sheep erythrocytes (from 10(4) to 10(8)) have been studied. In so doing two types of immune response can be isolated on the dependence upon the sensitivity threshold to antigen of DTHR and humoral immunity. Thus in C57BL/6 mice the antigen threshold for DTHR is of one time (in intraperitoneal immunization) or of a two times (in subcutaneous) lower order than for antibody response. In CBA line mice under subcutaneous immunization there can be seen quite an opposite picture while intraperitoneal immunization causes exact correlation of antigen threshold for cellular and humoral immune response.

UDC 616-056.3-092.9-092:612.017.1]-07