V. Ghisetti's research while affiliated with Ospedale Amedeo di Savoia and other places

Publications (17)

Article
Full-text available
The aims of this study were to characterize new SARS-CoV-2 genomes sampled all over Italy and to reconstruct the origin and the evolutionary dynamics in Italy and Europe between February and June 2020. The cluster analysis showed only small clusters including < 80 Italian isolates, while most of the Italian strains were intermixed in the whole tree...
Article
In the absence of effective prophylaxis and treatment, therapeutic options in HIV-positive patients with progressive multifocal leukoencephalopathy (PML) are limited to antiretroviral therapy: nevertheless, outcome is poor. We conducted a retrospective study (2009–2015) describing the outcome of 25 HIV-positive patients with detectable cerebrospina...
Article
Background: Lymphoproliferative disorders are frequently diagnosed in HIV-positive patients and severe infections may occur during antineoplastic treatments: the incidence and impact of such events are not well-characterized. Objective: To describe the occurrence and mortality of incident infections in HIV-positive individuals treated for lympho...
Article
Full-text available
Blood brain barrier (BBB) damage is a common feature in central nervous system infections by HIV and it may persist despite effective antiretroviral therapy. Astrocyte involvement has not been studied in this setting. Patients were enrolled in an ongoing prospective study and subjects with central nervous system-affecting disorders were excluded. P...
Article
Dengue fever is growing at a global level both as number of cases and as geographic area of endemicity. Italy is not in endemic area, but the competent vector Aedes albopictus is widespread in this country, so that the possibility of introduction of the infection cannot be ruled out. We retrospectively collected demographic, clinical, and laborator...
Article
Objectives: To evaluate the maintenance of virological suppression (VS) in antiretroviral-treated HIV-1-suppressed patients switching to a tenofovir/emtricitabine/rilpivirine (TDF/FTC/RPV) single-tablet regimen, by considering pre-existent resistance (pRes). Methods: pRes was evaluated according to resistance on all previous plasma genotypic res...

Citations

... PCR is the preferred way of diagnosis, but brain biopsy remains the gold standard when another disease entity such as CNS lymphoma is part of the differential diagnosis. Histopathologic triad of lytic demyelination of oligodendrocytes, bizarre astrocytes, and enlarged oligodendroglial nuclei are characteristic on brain biopsy or autopsy (58)(59)(60). ...
... [6] Risk factors associated to lymphoma's prognosis in PLWHIV include the International Prognosis Index (IPI) for Diffuse Large B-Cell Lymphoma (DLBCL), the Eastern Cooperative Oncology Group (ECOG) Performance Status, histologic subtype, cytomegalovirus (CMV) infection and CD4 low count. [7,8] Prior studies comparing the outcomes between HIV and non-HIV cohorts have reported inconsistent results. Two US studies found an association between low CD4, previous AIDS defining illnesses, and mortality in NHL. ...
... patients who did not respond to SOF-containing regimens, as reported in other studies.27,48 However, it is important to note that the mechanism of L159F and C316N resistance to SOF and other nucleos(t)ide analogue NS5B polymerase inhibitors remains unclear.49 ...
... Tale numero é in Italia veramente minimo, e in molti casi legato ad un errato approccio terapeutico legato alla diagnosi sbagliata del genotipo presente. Capita, ancor oggi, di constatare che circa il 5% dei fallimenti virologici siano legati ad un genotipo sbagliato, esito di una mancanza di ripetizione del test prima dell'inizio della terapia, o di un test che, per qualche ragione, ha dato un risultato non attendibile (7). É ragionevole ritenere che, con il costo odierno delle terapie (ancora intorno ai 10.000 Euro), e con le prospettive di eradicazione effettivamente ottenibili, la ripetizione del test del genotipo, i cui costi sono molto contenuti, rappresenti un elemento ancora necessario in una corretta procedura diagnostico-terapeutica finalizzata all'ottenimento del miglior risultato possibile. ...
... 9,33 This is not surprising given that (unexpectedly) only about half of naïve and one-quarter of ART-experienced study participants had an available GRT at baseline. This could be, at least in part, justified by the difficult recall of retrospective data, but it is worth stressing that RVP should not be used without an available GRT. 34 However, in the multivariable analysis, the unavailability of GRT was not significantly correlated with risk of discontinuation, while a history of previous failure increased the risk of discontinuation by 75%. ...
... Despite only 3 patients (5.5%) showed altered CSF S100beta values, the CSF levels of this astrocytosis marker moderately correlated with the duration in years of plasma viral suppression. This protein is a marker of astrocyte activation and BBB permeability, and it is expected to decrease with viral suppression and its consequent reduction in inflammation (Calcagno et al. 2017). Since the correlation refers to plasmatic HIV-RNA undetectability and not CSF viral load, it might reflect a chronic discrepancy between plasmatic viral control and CSF compartmentalization as already described in previous studies (Letendre et al. 2008). ...