Tsuneo Namba's research while affiliated with Toyama Medical and Pharmaceutical University and other places

Publications (334)

Article
Four new phenylpropanoid-substituted flavan-3-ols called apocynins A-D (1-4) have been isolated from the leaves of Apocynum venetum (Apocynaceae), together with two known phenylpropanoid-substituted flavan-3-ols, catechin- [8,7-e]-4α-(3,4-dihydroxyphenyl)-dihydro-2(3H)-pyranone (5) and cinchonain Ia (6), and four known flavan-3-ols, (-)-epicatechin...
Article
Foetidinol (1a), trinor-triterpenoid with a novel carbon skeleton, has been isolated from the rhizoma of Cimicifuga foetida L., and its structure was determined based on the 2D NMR spectroscopy including HMBC.
Article
Salvia deserta SCHANG. (Lamiaceae) is a plant grown in Xinjiang province in China, and its dried roots are called Xinjiang-Danshen. This plant has not been used as a medicine or a food, but recently it was reported that Xinjiang-Danshen is mixed in Danshen (roots of S. miltiorhiza Bunge), a well- known Chinese crude drug, at Xinjiang province when...
Article
The CHCl3 extract of Matteccia orientalis showed very strong hypoglycemic activity in streptozotocin (STZ)-induced diabetic rats. A new C-methyl flavanone derivative, 2'-hydroxymatteucinol (3) was isolated from the hypoglycemic activity bearing fraction, along with two known compounds, demethoxymatteucinol (1) and matteucinol (2). The structures of...
Article
Hot aqueous and methanol extracts of the rhizomes of Woodwardia orientalis were tested for their in vitro antiviral activity against herpes simplex virus type 1 (HSV-1), poliovirus type 1, and measles virus by plaque reduction assay. The aqueous extract of W. orientalis reduced the plaque forming ability of HSV-1 and poliovirus more strongly than d...
Article
Traditional medicines contain various metabolites derived from nucleic acid, protein, and lipid metabolism. Some of these specific metabolites may recognize the differences between viral and host metabolism resulting in anti-viral activity; hence traditional medicines may be useful sources for new antiviral agents. Traditional medicines can be chea...
Article
One new sesquiterpene named thotteodiol (1) with a novel skeleton and one new derivative of aristolochic acid named 7-hydroxyaristolochic acid III methyl ester (2), together with 20 known compounds, were isolated from the stems and roots of Thottea hainanensis. Their structures were determined by spectroscopic methods.
Article
Bezoar Bovis (BB:dried cattle gallbladder stones) has been used empirically in Asia for over 3000 years to treat heart and liver disorders. Yet its therapeutic potential remains unexplored by Western researchers. The aim of this study has been to clarify the actions of BB on cultured cardiomyocytes and to identify its active component(s). BB is a c...
Article
The cytotoxic activity against some tumor cell lines of 16 commonly used species of Asarum was evaluated in this study. All of these plants were widely used in Asian countries as traditional medicines or folk medicines. Their inhibitory activities against four tumor cell lines (HL-60, BGC-823, KB and Bel-7402) were compared. It was observed that 10...
Article
An HPLC method was developed for the simultaneous determination of five aristolochic acids (AAs) and two aristololactams (ALs) in the following six Chinese drugs derived from Aristolochia species. Samples were analyzed on a C(18) column with acetonitrile and 3.7 mm phosphoric acid buffer gradient elution, detected at 260 nm. Assay was linear over t...
Article
Aucubin was transformed to new nitrogen-containing compounds named aucubinines A and B, along with an aglycone, aucubigenin by anaerobic incubation with defined strains of human intestinal bacteria and with faecal flora of humans. Among 24 bacterial strains examined, Klebsiella pneumoniae, Bifidobacterium breve, Bifidobacterium pseudolongum, Peptos...
Article
To identify Radix Scutellariae (Huangqin) of different growth years, to distinguish whether it's wild or cultivated and to provide useful information for the quality control of Huangqin crude drug. By using morphological and histological methods, we studied 87 individuals of 45 specimens from 12 habitats of 5 provinces of China, which grew wild or...
Article
Taking Caulis Aristolochiae Manshuriensis (Guanmutong in Chinese, derived from the stem of Aristolochia manshuriensis) as an example, to study the affection of different preparations on the content of toxic constituents in traditional Chinese medicines. The separation was performed on a zorbax SB-C18 column with mobile phase of acetonitrile-3.7 mmo...
Article
In previous papers, we anatomically confirmed the botanical origins of the Chinese "Xuelianhua" and related crude drugs. They were shown to be derived from plants of subgenera Eriocoryne and Amphilaena of the genus Saussurea (Compositae). However, some samples circulated in the market were damaged by insects and/or shaking during transportation, an...
Article
The description of "Yinchenhao" in Chinese and Japanese medical and pharmaceutical literature, Bencao, was historically examined.Yinchenhao was consistently considered an indispensable medicine for treating jaundice. It was estimated that in China, a shrubby Artemisia plant just like the Artemisia capillaris, or a related plant, was utilized as Yin...
Article
To identify the botanical origin of the Guijiu in Shosoin of Japan from Tang Dynasty, and trace its medicinal history. Anatomical characteristics of the underground parts of Guijiu in Shosoin were compared with those of Hosta plantaginea and H. ventricosa, and research on the medicinal history of Guijiu was made based on its original identification...
Article
Eugeniin exhibits antiviral activity against acyclovir and phosphonoacetic acid (PAA)-resistant herpes simplex virus type 1 (HSV-1) as well as the wild-type HSV-1 in vitro. In this study, we characterized the biological activity of eugeniin in cutaneously HSV-1-infected mice and its interaction with HSV-1 DNA polymerase. The oral and intraperitonea...
Article
Swertiarum Herbae are used widely in the system of traditional medicines or folk medicines from India through Tibet to China and Japan. In order to clarify the botanical origin of "Zhangyacai" from Hunan Prov. and "Qingyedan" from Yunnan Prov., which are one of the common folk medicines in each province, respectively, an anatomical study was carrie...
Article
To identify the origin of a drug, "koku-oren" in Shosoin, 90% acetone extracts of "koku-oren" and of the underground parts of Picrorhiza kurroa and P. scrophulariiflora were analyzed by GC-MS. A characteristic peak was detected at 10.8 min in the gas chromatograms of "koku-oren" and of the underground parts of P. scrophulariiflora, which gave two c...
Article
An aqueous extract of Apocynum venetum leaves and its constituents inhibited thiobarbituric acid reactive substances (TBARS) and conjugated-diene formation in the Cu(2+)-induced oxidation of low density lipoprotein (LDL) in vitro. The TBARS formation was most strongly inhibited by chlorogenic acid with an IC(50) value of 1.9 microM, but other const...
Article
During a chemical study of Indonesian medicinal plants, we examined the constituents of fruits of Helicteres isora L. (Sterculiaceae), one of the famous Jamu medicines. From a H2O extract of the fruits, we isolated six new neolignans, the helicterins A – F (1 – 6), and elucidated their structures by spectral analyses. Helicterins A – F (1 – 6) are...
Article
Previously, 185 ribosomal RNA gene and matK gene sequences of Chinese herbal medicines, Ginseng Radix, Panacis Japonici Rhizoma and Panacis Quinquefolli Radix were shown to correspond with those of the original plants, Panax ginseng, P. japonicus and P. quinquefolius, respectively, with the species-specific sequences especially for 18S rRNA gene se...
Article
Effects of aqueous extracts of Apocynum venetum leaves (Luobuma extracts) on the blood pressure were evaluated in hypertensive animal models, such as spontaneously hypertensive rats (SHR), renal hypertensive rats and NaCl-induced hypertensive rats. In SHR, administration of Luobuma (heat-processed and unprocessed leaves) extracts at a dose of 70 mg...
Article
Essential oil composition of the fruit oils of Piper chaba, P. longum and P. nigrum were investigated by GC and GC/MS. Unlike P. nigrum oil, P. chaba and P. longum oils were found to contain few monoterpene hydrocarbons, a moderate content of sesquiterpenes and high content of aliphatic hydrocarbons. The content of caryophyllene oxide (7.4%) was th...
Article
The absorption, distribution and excretion of butylidenephthalide after dermal application to hairless mouse have been examined with [8-14C]butylidenephthalide. By the investigation of the whole body autoradiogram and liquid scintillation analysis, it was indicated that the transdermally applied butylidenephthalide quickly permeate into peripheral...
Article
Nitric oxide (NO) is one of the pro-inflammatory molecules. Some phenylethanoids have been previously shown to possess anti-inflammatory effects. Seven phenylethanoids from the stems of Cistanche deserticola, viz. isoacteoside, tubuloside B, acteoside, 2'-O-acetylacteoside, echinacoside, cistanoside A and tubuloside A, were tested for their effect...
Article
The leaves of Apocynum venetum L. are used as a tea material in north China and Japan. A water extract (500 mg/kg/day, one week administration) of the leaves of A. venetum showed protective effects against carbon tetrachloride (CCl4, 30 microliters/mouse) or D-galactosamine (D-GalN, 700 mg/kg)/lipopolysaccharide (LPS, 20 micrograms/kg)-induced live...
Article
The whole plant of Ixeris denticulata f. pinnatipartita and I. sonchifofia respectively afforded a new guaianolide sesquiterpene lactone glucoside named ixerin X, 8(,15-dihydroxy-1(10), 3,11(13)-guaiatriene-12,6-olide-15-O-β-D-glucopyranoside (1), ixerin Z, 1(10),3,11(13)-guaiatriene-12,6-olide-2-one-3-O- β -D-glucopyranoside (2), whose structure a...
Article
The active-oxygen scavenging activity of 70 traditional herbal medicines used in China and Japan as nourishing tonics were evaluated by electron spin resonance (ESR) technique, in order to evaluate their effectiveness for anti-aging and to search for new active-oxygen scavengers from natural resources. Most of the 70 herbal medicines showed scaveng...
Article
We investigated the effect of tetrahydroswertianolin (THS), a hepatoprotective agent from Swertia japonica, on tumor necrosis factor-alpha (TNF-alpha)-dependent hepatic apoptosis induced by D-galactosamine (D-GalN) (700 mg/kg, i.p.) and lipopolysaccharide (LPS) (10 microg/kg, i.p.) in mice. Apoptotic symptoms were observed at the initial stage of l...
Article
Rhus javanica, a medicinal herb, has been shown to exhibit oral therapeutic anti-herpes simplex virus (HSV) activity in mice. We purified two major anti-HSV compounds, moronic acid and betulonic acid, from the herbal extract by extraction with ethyl acetate at pH 10 followed by chromatographic separations and examined their anti-HSV activity in vit...
Article
We previously isolated berberine from aqueous extracts of tsu-kan-gan, a Kampo formula used for the treatment of osteoporosis. Berberine caused an inhibitory effect on parathyroid hormone (PTH)-stimulated bone resorption in neonatal mouse bone. In this report we describe the inhibitory effect of berberine on the formation of osteoclast-like multinu...
Article
During a chemical study of Indonesian medicinal plants, we examined the constituents of fruits of Helicteres isora L. (Sterculiaceae), one of the famous Jamu medicines. From a water extract of the fruits, we isolated three new neolignans, helisterculins A (1) and B (2) and helisorin (3), and elucidated their structures by spectral analyses. Heliste...
Article
In this paper, the botanical origins of the commercial crude drugs, 'sPang-spos' and related ethnomedicines, such as Chinese 'Gansongxiang,' and Ayurvedic 'Jatamansi,' were clarified by anatomical studies of the plant materials, N. chinensis and N. grandiflora, of the family Valerianaceae growing in Tibet and the neighboring countries. These two sp...
Article
From the rhizome of Smilax glabra Roxb., a new flavanone was isolated and named as smitilbin (1), together with 6 known compounds, engeletin (2), astilbin (3), dihydroquercetin (4), eurryphin (5), resveratrol (6), and 5-O-caffeoylshikimic acid (7). These compounds were applied to the assay of liver nonparenchymal cells (NPC) against hepatocytes (HC...
Article
Full-text available
Prolyl endopeptidase (PEP, EC 3.4.21.26) is an enzyme which plays a role in the metabolism of proline-containing neuropeptides, e.g., vasopressin, substance P and thyrotropin-releasing hormone (TRH), which have been suggested to be involved in learning and memory processes. In our systematic screening for PEP inhibitors from traditional Chinese med...
Article
We assessed the effect of acteoside, a naturally occurring antioxidative phenylethanoid, on hepatic apoptosis and the subsequent liver failure induced by D-Galactosamine (D-GalN) and lipopolysaccharide (LPS). A co-administration of D-GalN (700 mg/kg) and LPS (35 microg/kg) to mice evoked typical hepatic apoptosis characterized by DNA fragmentation...
Article
Full-text available
rights: 本文データは和漢医薬学会の許諾に基づき複製したものである
Article
Four water extracts of Kampo formulae (Yi-kkan-sen, Dai-ho-in-gan, Ni-chi-gan, Tsu-kan-gan) were screened for their inhibitory activities on bone resorption induced by parathyroid hormone (PTH) in organ culture using neonatal mouse parietal bones. Among the Kampo formulae, Tsu-kan-gan (TKG) showed the most potent inhibitory activity. We further fra...
Article
We have investigated the anti-metastatic effect of Celosia argentea seed extracts (CAE), which have traditionally been used as a therapeutic drug for eye and hepatic diseases in China and Japan. Intraperitoneal (i.p.) administration of CAE for 7 d before tumor inoculation significantly inhibited liver metastasis caused by intraportal injection of c...
Article
To study the flavonoids in fenugreek (Trigonella foenumgraecum) produced in China. The flavonoids were isolated with silica gel, polyamide and Sephadex LH-20 chromatography, and their structures were identified by physical, chemical properties and spectral analysis. Five flavonoid compounds were isolated from fenugreek seeds and identified as vitex...
Article
Tribulusamides A (1) and B (2), new lignanamides embracing two cinnamic amide parts joined in a cis configuration, were isolated from the fruits of Tribulus terrestris, together with four known compounds, N-trans-feruloyltyramine (3), terrestriamide (4), N-trans-coumaroyltyramine (5), and beta-sitosterol. The structures were elucidated by 2D-NMR sp...
Article
Full-text available
Traditional medicines contain various metabolites derived from nucleic acid, protein, and lipid metabolism. Some of these specific metabolites may recongnize the differences between viral and host metabolism resulting in antiviral activity ; hence traditional medicines may be useful sources for new antiviral agents. Traditional medicines can be che...
Article
Rhus javanica has been shown to exhibit anti-herpes simplex virus (HSV) activity and potentiate the anti-HSV activity of acyclovir in vitro and in vivo. This extract was examined for its suppressive efficacy on recurrent genital infection in guinea pigs. Guinea pigs were primarily infected intravaginally with HSV type 2 (HSV-2). Prophylactic oral a...
Article
Three new phenylethanoid glycoside, named scrosides A-C and a new iridoid glycosides, named picroside IV, have been isolated from the underground parts of Picrorhiza scrophulariiflora, together with 11 known compounds. Their structures were elucidated by the means of 2D NMR spectroscopy and chemical methods.
Article
We previously found that a methanolic extract of the stems of Sambucus sieboldiana inhibited bone resorption in organ culture. In this study, we further fractionated the methanol extract guided by the activity towards bone resorption stimulated by parathyroid hormone (PTH) in vitro. The ethyl acetate fraction (EtOAc Fr.) of the methanolic extract i...
Article
Comparative studies were carried out on the morphology and histology of 5 Clematis species, namely, C. intricata, C. aethusifolia, C. glauca, C. tangutica and C. hexapetala, allegedly used as "Tougucao". New characteristics, a sclerenchymatous ring composed of primary phloem fibers and lignified parenchyma cells, and a parenchymatous tertiary ring...
Article
The aerial parts of Conyza incana yielded three new diterpene acids: the two alicyclic furano diterpenes, namely, E,E and E,Z-incanic acids and an ent-labdane succinate ester. The structures of these compounds were elucidated by spectroscopic methods.
Article
Thirteen novel diarylheptanoids bearing a chalcone or a flavanone moiety (1-13), a new curcumin derivative, 1,2-dihydrobis(de-O-methyl)curcumin (14), and two known flavonoids (15 and 16) isolated from the seeds of Alpinia blepharocalyx K. Schum. were tested for their inhibitory effects on nitric oxide (NO) production in lipopolysaccaride (LPS)-acti...
Article
The whole plant of Ixeris sonchifolia afforded two new guaianolide sesquiterpene lactones named 8-desoxyartelin [3-hydroxy-1(10),3-guaiadiene-12,6-olide-2-one] and ixerin Z [1(10),3,11(13)-guaiatriene-12,6-olide-2-one-3-O-glucopyranoside], along with the known compound 9α-hydroxyzaluzalin C, whose structure and stereochemistry wee determined by spe...
Article
Four phenylethanoids isolated from the stems of Cistanche deserticola, acteoside (1), 2'-acetylacteoside (2), isoacteoside (3) and tubuloside B (4), significantly suppressed NADPH/CCl4-induced lipid peroxidation in rat liver microsomes. Addition of them to primary cultured rat hepatocytes efficiently prevented cell damage induced by exposure to CCl...
Article
The dry root and rhizome of Salvia miltiorhiza (Lamiaceae) are used as a crude drug Danshen, while those of S. deserta (Xinjiang-Danshen) are mixed in Danshen at Xinjiang province when the former is in short supply. The water and MeOH extracts of S. deserta showed strong aldose reductase (AR) inhibitory activity, and their active constituents were...
Article
The effect of spikelets of Miscanthus sinensis Andersson (M. sinensis) on IgE-mediated biphasic cutaneous reactions was investigated in BALB/c mice. Mice were passively sensitized by an intravenous (i.v.) injection of monoclonal antidinitrophenol IgE antibody (anti-DNP IgE mAb), or actively by an intraperitoneal (i.p.) injection of DNP-derivatized...
Article
Four unprecedented diarylheptanoids-calyxin H (1) and epicalyxin H (2), possessing a diarylheptanoid unit and a chalcone moiety, and blepharocalyxins A (3) and B (4), possessing two diarylheptanoid units and a chalcone moiety-were isolated from the seeds of Alpiniablepharocalyx. The structures of 1-4, including absolute stereochemistry, were elucid...
Article
The extracts of dried leaves of Apocynum venetum collected in different places in China (Luobuma I and II), and two processed leaves (Luobuma III and IV), were investigated on serum lipid and hepatic total cholesterol levels in cholesterol-fed rats. The serum total cholesterol levels were significantly reduced on day 40 in rats given the roasted le...
Article
Calyxins A (1), E (2) and F (3), 6-hydroxycalyxin F (4), Calyxin G and epicalyxin G (5 and 6), novel diarylheptanoids having a chalcone or a flavanone moiety, were isolated from Alpinia biepharocalyx K. Schum. and their structures, including the corrected one of calyxin A (1), were elucidated by spectroscopic methods.
Article
Effect of the long-term treatment with a Dan-Shen (Salviae miltiorrhizae radix) methanol extract (DME) or its major ingredient lithospermate B (LSB) on memory and learning in scenescence-accelerated mouse (SAM) was investigated by means of Morris's water maze task. DME or LSB treatment significantly decreased the escape latency in the P8 strain of...
Article
To investigate the permeability of natural compounds through hairless mouse skin, compounds having a range of lipophilicity, i.e., ginsenoside-Re (1), baicalein (2), glycyrrhizin (3), baicalein (4), wogonin (5), honokiol (6), magnolol (7), bergapten (8), shikonin (9) and sinomenine (10) were used. These compounds permeated through the skin a little...
Article
In order to develop convenient and reproducible methods for the identification of Ginseng drugs at a DNA level, PCR-Restriction fragment length polymorphism (PCR-RFLP) and Mutant allele specific amplification (MASA) analyses were applied, based on differences of the 18S rRNA gene sequence among three Panax species. The PCR product of each species o...
Article
Full-text available
The constituents of the MeOH extract of Salvia miltiorhiza BUNGE, which showed strong aldose reductase (AR) inhibitory activity, were examined, and two new abietane-type diterpenoids, danshenol A (1) and danshenol B (2), were isolated together with six known ones: dihydrotanshimme I (3), cryptotanshinone (4), tanshinone I (5), tanshinone IIA (6), (...
Article
Celosian, an acidic polysaccharide from the seeds of Celosia argenteo (Amaranthaceae) was found to be a potent antihepatotoxic agent for chemical and immunological liver injury models in animals. The immunomodulating action of celosian was studied to clarify the preventive mechanism of celosian on liver injuries. Celosian induced tumor necrosis fac...
Article
Rabdosia trichocarpa is used in a popular home-made remedy for gastric and stomachic complaints in Japan. A diterpene, trichorabdal A from R. trichocarpa, showed a very strong in vitro antibacterial activity against Helicobacter pylori. With regard to the antibacterial activity of the extract and constituents of R. trichocarpa against H. pylori, th...