Tiantao Zhao’s research while affiliated with Chongqing University of Technology and other places

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Publications (52)


Characteristics of Biofilm Layer in a Bio-doubling Reactor and Their Impact on Aerobic Denitrifying Bacteria Enrichment
  • Article

December 2024

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2 Reads

Environmental Research

Tianrui Zhai

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Lijie Zhang

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Fupan Zhang

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[...]

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Tiantao Zhao


Continuous operational performance of MBR. a NH4⁺-N removal; b COD removal; c TN removal
Microbial community structure of MBR
Biodiversity of the biological amplifier after three months of continuous operation
One-time inoculation of TA-1 enhances the MBR. a Inoculation with 2.5% TA-1; b Inoculation with 5% TA-1; c Inoculation with 10% TA-1
Effectiveness of biological amplifier coupled with HN-AD in enhancing MBR for synthetic wastewater treatment a COD removal; b NH4⁺-N removal; c TN removal

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Research on the application of heterotrophic nitrification-aerobic denitrification bacteria in membrane bioreactor (MBR)
  • Article
  • Publisher preview available

September 2024

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6 Reads

Biotechnology Letters

Inoculating heterotrophic nitrification-aerobic denitrification bacteria (HN-AD) to enhance membrane bioreactor (MBR) efficiency may result in the loss of functional bacteria. Therefore, this study compares the application results of enhancing MBR with a self-designed biological amplifier coupled with HN-AD against the performance of conventional MBR. After enhancement, the MBR achieved a removal efficiency of 96.7% for NH4⁺-N (100 mg/L) and 96.4% for COD (400 mg/L) in synthetic wastewater. There was a 33% increase in TN (100 mg/L) removal efficiency. The dominant bacteria in the MBR were Alcaligenes (48.4%) and Thauera (15.2%). Additionally, the abundance of denitrification genes (nirK, norB, nosZ) increased in the enhanced MBR, contributing to improved TN removal efficiency. The use of a biological amplifier effectively solved the problem of HN-AD loss in sewage treatment.

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Research on the Application of Heterotrophic Nitrification-Aerobic Denitrification Bacteria in Membrane Bioreactor (MBR)

March 2024

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22 Reads

The inoculation of heterotrophic nitrification-aerobic denitrification bacteria (HN-AD) in MBR facilitates the simultaneous nitrification and denitrification process. However, many HN-AD will be lost during operation. Therefore, this study compares the application results of enhancing MBR with a self-designed biological amplifier coupled with HN-AD against the performance of conventional MBR. Post-enhancement, the MBR achieved removal efficiency of 96.7% for NH 4 ⁺ -N (100 mg/L), 96.4% for COD(400 mg/L) in synthetic wastewater, with a 33% increase in the removal efficiency of TN(100 mg/L). Before enhancement, the dominant denitrifying genus of MBR was Thauera, with the highest abundance of 56.1%. After enhancement, the dominant bacterial in the MBR were Alcaligenes (48.4%) and Thauera (15.2%). In addition, the abundance of denitrification genes (nirK, norB, nosZ) increased in the enhanced MBR, which contributed to an improved TN removal efficiency. The use of biological amplifier effectively solved the problem of HN-AD loss in sewage treatment.







Kinetic characteristics of degradation of CB under different conditions
Kinetic analysis and Dual biodegradation pathway for chlorobenzenes removal by Serratia marcescens strain TF-1 and its performance in contaminated soil

October 2023

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34 Reads

Microorganisms with specific functions have significant potential for use in the remediation of contaminated sites. In the past, this study investigated the optimal growth conditions and chlorobenzene (CB) degradation capability of the Serratia marcescens strain TF-1, a strain known for its CB degradation abilities, and its application in soil remediation projects. Under laboratory conditions, TF-1 exhibited its highest CB degradation capability and cell density at pH 7, with a 5% inoculum size, and a temperature of 30°C. The results indicated that TF-1 possessed robust pH tolerance and was suitable for use in contaminated soils with varying pH levels. Within a concentration range of 20–200 mg·L − 1 , TF-1 demonstrated a CB assimilation and degradation rate ranging from 0.32 to 1.65 mg·L − 1 ·h − 1 , which was 1–6 times higher than that of known CB-degrading bacteria. Furthermore, the study confirmed that TF-1 could co-metabolically degrade CB and 1,2-dichlorobenzene (1,2-DCB) using sodium succinate and sodium citrate as substrates, with significantly higher co-metabolic degradation rates compared to assimilative degradation rates. The addition of TF-1 and a remediation agent composed of sodium succinate to the contaminated soil enabled the removal of various chlorobenzene pollutants. Comparative diversity analysis before and after remediation demonstrated that TF-1 successfully colonized the contaminated soil and enriched the local microorganisms, such as Azoarcus , in the co-degradation of pollutants. As a novel CB-degrading bacterial strain, TF-1 had been applied for the first time in situ in the remediation of CB-contaminated soil, providing valuable theoretical guidance and practical experience for in situ CB-contaminated soil remediation techniques.


Citations (36)


... CYP450s are classified under the oxygenase group of enzymes, which are capable of transferring electrons to oxygen and facilitating the oxidation of external substances, including heavy metal stress, toxins, and environmental pollutants (Lin et al., 2022;Zhang et al., 2024). When exposed to 1.25 mg/L Cu, a total of 25 CYP450 genes showed significant differences in transcriptional levels (15 were upregulated and 10 were downregulated; Table S7-2) in T. hirsuta AH28-2 compared with the control group. ...

Reference:

Transcriptome analysis reveals the mechanism of tolerance to copper toxicity in the white rot fungus Trametes hirsuta AH28-2
Genetic response analysis of Beauveria bassiana Z1 under high concentration Cd(II) stress
  • Citing Article
  • November 2023

Journal of Hazardous Materials

... After deep fertilization, the distribution of NH 4 + −N and NO 3 − −N in the soil changed, and the contents of NH 4 + −N and NO 3 − −N in shallow soil reduced [9], while the shallow soil was the main area of methane absorption [18]. (2) Plant root exudates can affect the methane absorption process, and methanotrophs show greater methane oxidation capacity in the rhizosphere [35]. After deep fertilization, the nutrients concentration of deep soil increased, which can adjust the vertical distribution of roots, increase the proportion of roots in deep soil [36], improve rhizosphere oxygen utilization, increase soil methane oxidation levels, consume methane, reduce methane emissions, or promote methane absorption [18]. ...

Metabolic diversity shapes vegetation-enhanced methane oxidation in landfill covers: Multi-omics study of rhizosphere microorganisms
  • Citing Article
  • October 2023

Waste Management

... The antiinflammatory, anti-oxidant, and antibacterial activity of the fabricated sample was studied. The wound healing of almost 99.7% was achieved using rat models [31]. Composite of PVA/CMC/hydroxypropyl methylcellulose (HPMC)/Ormocarpum cochinchinense extract was fabricated by solvent casting techniques. ...

Preparation and evaluation of Thesium chinense extract loaded polyvinyl alcohol/sodium alginate hydrogel for wound healing

Polymer Bulletin

... Alternatively, at the genus level, some fungi such as Phoma and Penicillium were found to have pathogenic characteristics that may be associated with fruit spoilage [45,46]. Sphingomonas, Bacillus, and Streptomyces are the dominant genera in soils, which is similar to other studies [47][48][49]. ...

Investigation of microbial community structures in typical landfill cover soils of China
  • Citing Article
  • January 2022

International Journal of Environment and Pollution

... Tan et al. (2020) reported that HNADB played a prominent role in nitrogen removal in two pilot-scale TFCWs, achieving impressive NH 4 + -N and TN removal efficiencies ranging from 70.9 % to 91.8 % and 72.6 % to 95.5 %, respectively. Furthermore, in a study by Chen et al. (2023), HNADB underwent five generations of domestication and demonstrated remarkable efficacy in removing 98 % of NH 4 + -N at an original concentration of 400 mg⋅L − 1 . Therefore, the excellent removal of NH 4 + -N could be due to the prevalence of HNADB in TFCW-S. ...

Domestication and pilot-scale culture of mixed bacteria HY-1 capable of heterotrophic nitrification-aerobic denitrification
  • Citing Article
  • June 2023

Bioresource Technology

... They also display anti-inflammatory, antioxidant, and antipruritic effects, significantly enhancing wound healing. Incorporating low concentrations of notoginsenosides into hydrogels has been shown to enhance biocompatibility, optimize wound conditions, strengthen antibacterial activity, and accelerate wound closure [48,49]. ...

Preparation and characterization of Panax notoginseng saponins loaded hyaluronic acid/carboxymethyl chitosan hydrogel for type o diabetic wound healing
  • Citing Article
  • December 2022

Materials Today Communications

... Alcaligenes faecalis NR exhibited the capacity to eliminate 300 mg/L of NH4 + -N within a 24 h period [52]. Furthermore, Alcaligenes faecalis TF-1 exhibited the ability to remove 52.87 mg/L of NH4 + -N in environments characterized by elevated salinity levels [53]. Furthermore, Alcaligenes faecalis WT14 exhibited the ability to attain a TN removal rate of 99.3% in simulated sewage under optimal conditions [6]. ...

Nitrogen removal and mechanism of an extremely high-ammonia tolerant heterotrophic nitrification-aerobic denitrification bacterium Alcaligenes faecalis TF-1
  • Citing Article
  • July 2022

Bioresource Technology

... This has made nitrogen (N) and phosphorus (P) the most common pollutants in water bodies [2]. These pollutants contribute to eutrophication and severe ecological problems, posing risks to human health and restricting the sustainable development of the aquaculture industry [3]. Therefore, the development of effective, environmentally friendly, and economical methods for removing N and P from aquaculture wastewater remains a significant challenge. ...

Characterization of a novel salt-tolerant strain Sphingopyxis sp. CY-10 capable of heterotrophic nitrification and aerobic denitrification
  • Citing Article
  • May 2022

Bioresource Technology

... The dominance of Pseudomonas was most likely driven by the presence of multiple copies of benzene-degrading genes and genes encoding GST, as well as various proteins that have been predicted to be associated with a high pH preference. 44 It has been documented that GST effectively promotes the oxidation and detoxification of CAHs by conjugating with glutathione, 16 thereby enhancing the resilience and adaptability of Pseudomonas under severely polluted environments. The ability to utilize benzene and the preference for high pH further promoted the growth of Pseudomonas in this cluster. ...

Direct aerobic oxidation (DAO) of chlorinated aliphatic hydrocarbons: A review of key DAO bacteria, biometabolic pathways and in-situ bioremediation potential
  • Citing Article
  • April 2022

Environment International

... Chlorinated hydrocarbons (CHCs) are extensively used solvents in petrochemical industries which accordingly make them common groundwater contaminant [1][2]. Trichloroethane (TCA) is considered one of the most toxic pollutants that is discharged into water sources and affects human health that is known to be carcinogenic if one is subjected to it for long periods [3]. ...

Cometabolic degradation mechanism and microbial network response of methanotrophic consortia to chlorinated hydrocarbon solvents
  • Citing Article
  • January 2022

Ecotoxicology and Environmental Safety