Tianlai Li’s research while affiliated with Shenyang Agricultural University and other places

What is this page?


This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.

Publications (178)


Genome-Wide Identification and characterization of the Thioredoxin (TRX) Gene Family in tomato (Solanum lycopersicum) and a functional analysis of SlTRX2 under salt stress
  • Article

March 2025

·

14 Reads

Plant Physiology and Biochemistry

Xiaoyu Cui

·

Jiamao Gu

·

Pengkun Liu

·

[...]

·

Tianlai Li


Shannon and richness indexes of root endophytic abundant bacteria (A,E), rare bacteria (B,F), abundant fungi (C,G), and rare fungi (D,H) under different treatments. CKP0, Unfertilized; CKP1, Chemical phosphorus applied; CNP0, Chemical nitrogen applied; CNP1, Chemical nitrogen plus phosphorus; ONP0, Organic manure supply nitrogen; ONP1, Organic manure supply nitrogen plus phosphorus. *p < 0.05, as tested by multiple comparisons using the Kruskal–Wallis test, and only significant differences are displayed. The treatment denotations are the same as those in Figure 1.
Subcommunity composition at the phylum level and non-metric multidimensional scaling (NMDS) plots of root endophytic abundant bacteria (A,E), rare bacteria (B,F), abundant fungi (C,G), and rare fungi (D,H) under different treatments. Unclassified bacterial and fungal OTUs were labeled as “Others.” The treatment denotations are the same as those in Figure 1.
Root endophytic bacterial and fungal subcommunities assembly processes (based on βNTI and RCBray). βNTI of root endophytic abundant bacteria (A), rare bacteria (B), abundant fungi (C), and rare fungi (D) subcommunities. Proportions of deterministic and stochastic assembly processes in governing root endophytic abundant bacteria (E), rare bacteria (F), abundant fungi (G), and rare fungi (H) subcommunities under different treatments.
Co-occurrence networks of bacterial and fungal coexistence communities under different fertilizations (A–F). The networks were established by calculating correlations among abundant, rare, and other OTUs.
Distributions of network roles by analyzing module features, within-module connectivities (Zi) and among-module connectivities (Pi), in the endophytic abundant bacterial (A), rare bacterial (B), abundant fungal (C), and rare fungal (D) co-occurrence networks of root, respectively.

+2

The process of nitrogen-adaptation root endophytic bacterial rather than phosphorus-adaptation fungal subcommunities construction unveiled the tomato yield improvement under long-term fertilization
  • Article
  • Full-text available

January 2025

·

5 Reads

Interactions between endophytes (endophytic bacteria and fungi) and plants are crucial in maintaining crop fitness in agricultural systems, particularly in relation to abundant and rare subcommunities involved in community construction. However, the influence of long-term fertilization on heterogeneous rhizosphere nitrogen and phosphorus environments and how these conditions affect the key subcommunities of root endophytes and their community assembly mechanisms remain unclear. We studied the 26th year of a field experiment conducted in a greenhouse with varying levels of nitrogen and phosphorus (CKP0, CKP1, CNP0, CNP1, ONP0, and ONP1) to assess the composition of tomato root endophytes and their impact on yield. We employed 16S rRNA and fungal ITS region amplicon sequencing to investigate the assembly mechanisms of abundant and rare endophytic subcommunities, network correlations, core subcommunity structures, and key species that enhance crop yield. The results indicated that organic manure and phosphorus fertilizers significantly increased the rhizosphere soil nitrogen content, phosphorus content, and phosphorus availability (labile P, moderately labile P, and non-labile P). These fertilizers also significantly affected the composition (based on Bray-Curtis distance) and community assembly processes (βNTI) of endophytic microbial subcommunities. The assembly of both bacterial and fungal subcommunities was primarily governed by dispersal limitation, with community structures being significantly regulated by the content of rhizosphere soil available nitrogen (AN) and moderately labile P (MLP). Rare bacterial and fungal subcommunities complemented the ecological niches of abundant subcommunities in the co-occurrence network, supporting community functions and enhancing network stability. Nitrogen-adapting abundant and rare bacterial subcommunities provided a stronger predictive correlation for tomato yield than phosphorus-adapting fungal subcommunities. Additionally, three core genera of rare endophytic bacteria such as Arthrobacter, Microbacterium, and Sphingobium were identified as potentially involved in improving crop yield improvement. These findings revealed the distinct assembly mechanisms of endophytic microbial subcommunities affected by fertilization, enhancing our understanding of better management practices and controlling endophytes to improve crop yield in intensive agricultural ecosystems.

Download

A regulatory network involving calmodulin controls phytosulfokine peptide processing during drought-induced flower abscission

January 2025

·

23 Reads

The Plant Cell

Drought stress substantially decreases crop yields by causing flowers and fruits to detach prematurely. However, the molecular mechanisms modulating organ abscission under drought stress remain unclear. Here, we show that expression of CALMODULIN2 (CaM2) is specifically and sharply increased in the pedicel abscission zone (AZ) in response to drought and plays a positive role in drought-induced flower drop in tomato (Solanum lycopersicum). Due to partial functional redundancy with SlCaM6, we generated the Slcam2 Slcam6 double mutant, which showed minimal flower drop under drought. SlCaM2 and SlCaM6 interacted with the transcription factor Signal responsive 3L (SlSR3L), with the three proteins operating in the same pathway, based on genetic data. We identified Protease inhibitor26 (SlPI26) as a target gene of SlSR3L by DNA affinity purification sequencing (DAP-Seq) and transcriptome analysis. SlPI26 specifically inhibited the activity of the phytaspase SlPhyt2, hence preventing the generation of active phytosulfokine peptide and negatively regulating drought-induced flower drop. SlCaM2 and SlCaM6 enhanced the repression of SlPI26 expression by SlSR3L, promoting drought-induced flower drop. In addition, the Non-phototropic hypocotyl3 (SlNPH3)–Cullin3 (SlCUL3) complex, which relies on auxin, interacted with SlSR3L to induce its degradation. However, under drought conditions, SlNPH3–SlCUL3 function is compromised due to lower auxin concentration. These results uncover a regulatory network that precisely controls floral drop in response to drought stress.


Study on a transversal–longitudinal ventilation structure for the caged chicken house

November 2024

·

38 Reads

In the trend toward low-carbon urban development, optimizing the ventilation systems of agricultural facilities is crucial for reducing energy consumption and pollutant emissions. In this study, a combination of transversal and longitudinal ventilation was proposed to improve the uniformity and stability of airflow in caged chicken houses in winter and minimize cold stress in chickens. Meanwhile, the synergistic coupling between the structural and ventilation parameters of the chicken house was thoroughly analyzed to address the inefficiency of the airflow path. Innovative optimization of fan height, eave height, ridge angle and vent angle was carried out through a combination of experimental measurements and numerical simulations, scaled physical models and CFD experiments. By evaluating the ventilation efficiency, pollutant emission efficiency, relative energy savings, temperature and humidity variations, and airflow uniformity, the study shows that the optimized configuration significantly improves ventilation performance and energy efficiency. The optimized configuration resulted in energy savings of 17.85% for fan height, 27.97% for vent angle, 26.05% for eave height and 15.49% for ridge angle. Unlike previous studies focusing on a single parameter or ventilation method, this study analyzed multiple parameters and ventilation methods in a synergistic and coupled manner. This research approach provides new perspectives on the design of ventilation systems for caged chicken houses and contributes to the environmental sustainability of poultry farming. By reducing energy consumption and pollutant emissions, this study provides valuable insights and practical references for sustainable urban development. Graphical Abstract Ventilation Design in Caged Chicken Houses




Manipulation of artificial light environment improves plant biomass and fruit nutritional quality in tomato

November 2024

·

54 Reads

·

1 Citation

Journal of Advanced Research

Introduction: The yield and quality of tomato (Solanum lycopersicum. L) are often decreased when plants suffer from low light intensity and short-photoperiod in winter. Manipulation of the artificial light environment is a feasible technology to promote off-seasonal production and improve fruit nutritional quality in the greenhouse. Objectives: Here we aim to investigate the appropriate balance between red (R) and blue (B) light to improve tomato yield and quality traits. Methods: Biochemical, molecular and genetic analysis were used to study the photosynthetic traits, pigments, sugar and volatile accumulation pathway genes. Results: Tomato plants subjected to R1B0.8, a ratio between monochromatic red (R) and blue (B) light, for 16 h photoperiod showed significantly increased chlorophyll and biomass accumulation compared to white (W) light treatment. R1B0.8 light treatment enhanced electron transfer efficiency and photosynthetic capacity by improving the light energy utilization rate and inducing photosystem core subunit genes (SlPsaC, SlPsaB, SlPsaA) and light-harvesting complex genes (SlLHCB/A). Compared to W light, R1B0.8 light also induced carotenoid accumulation and accelerated fruit ripening, which was associated with the upregulation of carotenoid biosynthesis genes (SlPSY1, SlPDS) and ethylene biosynthesis genes (SlACS2, SlACO2) in tomato fruits. Moreover, fruits exposed to R1B0.8 light treatments significantly improved fructose and glucose accumulation and the expression of the volatile-related gene (SlAADC1a) and flavor-related gene (SlGORKY). Conclusion: Our results showed that R1B0.8 light with a 16 h photoperiod could prominently promote photosynthetic traits, pigments, sugar and volatile accumulation in tomato. Our findings on the manipulation of artificial light environments in protected horticulture offer possibilities for enhancing crop yield and quality to meet the increasing global demand for food.




Citations (63)


... For quantitative PCR (qPCR) experiments, stem tips from the five experimental lighting conditions were taken at noon on the 21st day. To determine changes in gene expression over time within a treatment, leaf samples were collected at 14 , with quality assessment performed through agarose gel electrophoresis and analysis with a K2800 nucleic acid analyzer (Kaiao, Beijing, China). Subsequently, the RNA was reverse-transcribed into cDNA using Script III All-in-one RT mix with dsDNase kits (Huaxingbio, Shenyang, China). ...

Reference:

Intermittent Supplementation with Far-Red Light Accelerates Leaf and Bud Development and Increases Yield in Lettuce
Manipulation of artificial light environment improves plant biomass and fruit nutritional quality in tomato

Journal of Advanced Research

... A link between the response to salt stress and heat has been shown previously, as heat shock proteins enhance resistance to salt stress and, conversely, overexpression of salt stress proteins provides resistance to heat stress (Azameti et al., 2024;Chaffai et al., 2024;Chang et al., 2024). The relationship of salt stress response to leaf senescence, hypoxia, water transport, responses to blue, red, and far-red light, reactive oxygen species, dehydration, abscisic acid, salicylic acid, and jasmonic acid has also been demonstrated in experiments (Serraj et al., 1994;Szepesi et al., 2009;Khan et al., 2012;Kumar et al., 2014;Joseph, Jini, 2010;Sharma et al., 2022;Kesawat et al., 2023;Lu, Fricke, 2023;Tan et al., 2023;Peng et al., 2024). ...

Revisiting the role of light signaling in plant responses to salt stress

Horticulture Research

... However, despite repeated tries, we were unable to obtain an effective AsODN to inhibit the expression of CsCSN5 in tea plants. In a recent study, SlCSN5A was involved in regulating the stability of chloroplast proteins and negatively regulated the tolerance of tomato plants in response to low temperature stress at night (Lu et al. 2024). We speculated that CsCSN5 may have similar function, warranting further investigation. ...

Ubiquitin-mediated degradation of SlPsbS regulates low night temperature tolerance in tomatoes
  • Citing Article
  • September 2024

Cell Reports

... Generally, the overexpression of WRKY transcription factor genes can enhance the synthesis of metabolites and bolster resistance to both biotic and abiotic stresses by elevating the expression levels of target genes [30]. Notably, the study demonstrated that the overexpression of the tomato SlWRKY6 gene significantly upregulates the expression of downstream target genes such as CAT1, CAT2, CSD1, and GSH1, which in turn improves the plant's catalase (CAT) enzyme activity and tolerance to heavy metal stress [38]. Furthermore, Chen et al. reported that when tea trees are subjected to anthracnose pathogen attacks, the expression of WRKY proteins is activated, prompting the tea trees to produce substantial amounts of volatile organic compounds in response to the pathogen's assault [39]. ...

Overexpression of SlWRKY6 enhances drought tolerance by strengthening antioxidant defense and stomatal closure via ABA signaling in Solanum lycopersicum L
  • Citing Article
  • June 2024

Plant Physiology and Biochemistry

... Sharafi et al. 66 reported that ameliorating chilling damage in tomato fruits by melatonin treatment might be attributed to boosting GABA shunt activity represented by higher GAD, GABA-T, and SSADH activity concurrent with boosting phenylpropanoid pathway activity represented by higher PAL expression and activity leading to higher phenols biosynthesis and higher DPPH radical scavenging activity. Wang et al. 68 reported that suppressing THM27 transcription factor expression during cold stress might be crucial for ameliorating chilling damage in tomato plants by activating GAD2 expression and boosting endogenous GABA biosynthesis, which promotes anthocyanins biosynthesis and ROS scavenging activity. Therefore, GABA shunt activity revealed by higher GABA-T expression and activity could ameliorate chilling damage in banana fruits by 150 µM dopamine treatment by avoiding intracellular ROS overaccumulation and supporting intracellular ATP and carbon skeleton availability. ...

SlGAD2 is the target of SlTHM27, positively regulates cold tolerance by mediating anthocyanin biosynthesis in tomato
  • Citing Article
  • April 2024

Horticulture Research

... Additionally, the materials and technologies used in greenhouses allow for the passive manipulation of incident sunlight, promoting the transmission of an ideal light spectrum for crop growth (Feng et al., 2024;Shi et al., 2024). Such a practice not only increases crop yields but also facilitates the management of the internal microclimate of agricultural greenhouses, resulting in reduced energy consumption (Mishra et al., 2023). ...

Performance of Reflective Film on the Light Environment of Chinese Solar Greenhouse

Agronomy

... Many studies have found that WRKY TFs are involved in the regulation of low temperature response in plants. It has been found that CsWRKY46 in cucumber (Cucumis sativus) [18], OsWRKY71 in rice (Oryza sativa) [19], VvWRKY28 in grape (Vitis Vinifera) [20], PmWRKY57 in Prunus mume [21], SlWRKY50 in tomato (Solanum lycopersicum) [22] and ShWRKY55 in wild tomato (Solanum habrochaites) [23] are associated with enhanced cold tolerance. Therefore, we hypothesize that WRKY genes regulating cold tolerance may also be present in elephant grass. ...

ShWRKY55 enhances the cold resistance of wild tomato LA1777 by regulating the expression of the key gene ShSAMDC2 involved in polyamine synthesis
  • Citing Article
  • March 2024

Environmental and Experimental Botany

... The Chinese solar greenhouse (hereafter referred to as 'solar greenhouse') is widely used for vegetable production in northern China with little or no additional heating during the winter months [1,2]. It plays a crucial role in ensuring a consistent vegetable supply throughout this critical period [3][4][5][6][7]. Moreover, heavy snowfall is a major cause of structural damage and failure in solar greenhouse frames [8,9]. ...

Utilization of Surplus Air Thermal Energy by a Water Cycle System in a Chinese-Type Solar Greenhouse

Agronomy

... However, model simulations suggest that this enhancement in Ac from increased canopy height is less evident in densely planted or horizontally uniform canopies [13]. Consequently, many studies have focused on alternative planting strategies, such as modifying planting patterns [14] and adjusting leaf area index [15] to improve Ac, with comparatively less attention given to increasing crop height. In subtropical and tropical regions, taller greenhouses and highwire cultivation are often considered strategies primarily for mitigating excess summer temperatures rather than for enhancing Ac [16][17][18][19]. ...

Estimating the Light Interception and Photosynthesis of Greenhouse-Cultivated Tomato Crops under Different Canopy Configurations

Agronomy

... Morchella exhibits three distinct modes of sexual reproduction: heterothallism, pseudohomothallism, and unisexual reproduction [3]. Morel mushrooms have a delicate flavor and aroma, making them a gourmet product [4,5]. Several studies emphasize their importance as a nutraceutical, with significant bioactive properties [4,6]. ...

Comparative analysis of the nutritional and biological properties between the pileus and stipe of Morchella sextelata