Thomas Van Gansbeke’s research while affiliated with Rousselot and other places

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Publications (5)


Extracellular matrix mechanical modulation of lung mesenchymal stromal cells improves the capacity for supporting tissue repair in COPD
  • Conference Paper

May 2025

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5 Reads

Lei Wang

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Whole Earth Finance: Beyond Bretton Woods Solutions for the 21st Century
  • Preprint
  • File available

April 2025

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51 Reads

How can systemic changes in the international financial architecture accelerate the world's transition to clean energy and address the urgent challenges of the polycrisis? Building on extensive conversations within the Beyond Bretton Woods network, we present a series of causal relationships to diagnose the impairment if not hijacking of modern capitalism. Based on recent discourse about planetary boundaries and on sound applications of economics, we then detail six high-impact recommendations whose time has come: placing ecocentrism at the center of international finance reform; implementing an instantaneous global carbon price; establishing a new debt restructuring mechanism; incorporating the polycrisis into monetary policy; designing a novel nature-based currency; and creating new global governance entities. We conclude with a brief discussion of the means by which civil society can be rallied in support of these systemic changes.

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Library of the mechanical properties of the different GelMA hydrogel formulations and how they affect volumetric printing parameters. A) Schematic representation of the GelMA reaction process and overview of the different parameters involved in the design and tuning of the final bioink or bioresin mechanical properties. Representative (n = 3) photo‐rheology curves for the 90 kDa and the 160 kDa GelMAs with different degrees of modification (40%, 60%, and 80%), at different polymer concentrations (5% w/v, 10% w/v, 15% w/v, and 20% w/v). B) Experimental setup for computational axial lithography, showing a volumetric printed pancreas model. C) Printing light dose comparison between optimal values specific for the different GelMA formulations (5%w/v), and a reference value (90p80 optimal light dose). D) The hydrogel strengths of the various GelMA formulations at different concentrations (values of G’ after 30 seconds of exposure). An increased degree of modification leads to greater hydrogel strength. E) The relationship between hydrogel storage modulus and concentration is different depending on the average molecular weight (values of G’ after 30 seconds of exposure). For the 160 kDa GelMA, with increasing concentration, the storage modulus scales follow a linear trend. Conversely, at lower MW (90 kDa), storage modulus scales follow a power law, as a function of the concentration (n = 3). F) 3D overview of the storage moduli (G’) distribution of the GelMA hydrogels with 160 kDa MW i) and 90 kDa MW, as determined through photo‐rheology (n = 3).
Effect of temperature on volumetric printing parameters, on the final mechanical properties and cross–linking kinetics of different GelMA hydrogels. A) Higher cross–linking temperatures lead to higher optimal printing light doses. Printing light dose comparison between optimal values specific for the different GelMA formulations (5%w/v) printed at room temperature, and a reference value (optimal values specific for the different GelMA formulations printed at 4 °C). B) i) Storage moduli (G’) of 160p60, 90p60, and 90p40 at 5% w/v at 4 °C, 21 °C and 37° (n = 3), showing that photo‐curing at decreased temperature results in increased hydrogel stiffness. ii) Photo‐cross–linking rates represented by the first derivatives of the photo‐rheology curves of 160p60, 90p60, and 90p40 at 5% w/v at 4 °C, 21 °C and 37° (n = 3), showing how with increasing temperature, the curing rates slow down. Photorheology was performed with 10 mW cm⁻², 300 mJ cm⁻² light sources.
Optimizing 3D cell culture environments via tuning of the GelMA properties via molecular weight and degree of functionalization modulation and targeting the mechanical profile of soft tissues. A) storage moduli at the 7 min mark (G′) B) Compressive mechanical properties and C) the stress relaxation % after 2 min for the different GelMA formulations at 5% w/v concentration (n = 4). D) Recapitulating an optimal environment for the iβ‐cell line leveraging 90p60 GelMA mechanical properties. i) Effect of molecular weight of GelMA bioresins (90p60 and 160p60) at 5% w/v concentration (1%w/v LAP, dissolved in PBS 1X) on ii) ability to proliferate and form islet‐like clusters iβ‐cell line and iii) metabolic activity (n = 4). Scale bars: 500 µm E) i) Effect of selected salient macromolecules from the pancreatic ECM on ii) metabolic activity, iii) overall, and iv) fold‐increment normalized luciferase secretion of iβ‐cell line volumetric printed in GelMA 90p60 (n = 4). Scale bar: 300 µm.
Sequential multi‐material volumetric printing. A) Examples of complex structures obtained via volumetric bioprinting of GelMA formulations with different molecular weights, including two interlocked rings, and a model of a hand with pinched fingers. Quantitative printing accuracy 3D maps are provided as a difference between the original STL file and the printed object, showing local variations in the size of the printed features across the whole volume of the objects. Scale bars = 10 mm. B) Graphical overview of the sequential multi‐material volumetric printing process, and demonstration of two printed independent and intertwined hollow cube scaffolds (digitally colored in grey and cyan in the 3D light sheet images to facilitate visualization). Scale bar = 3 mm C) Example of multi‐material prints: i) a dual material printed reinforced gyroid (Scale bar = 4 mm) and ii) a dual material printed screw (Scale bar = 5 mm) with a core and thread printed with formulations embedding iβ‐cells with different stainings (DiL and Did. Vybrant Multicolor Cell‐Labeling Kit, Thermo Fischer Scientific, The Netherlands).
Low molecular weight gelatins enable seamless and omnidirectional extrusion printing as suspension baths, and Embedded extrusion Volumetric Printing (EmVP). A) Fluid bulk support baths made of 90 kDa gelatin and/or GelMA (see Figure S9) show self‐healing‐like properties, whereas 160 kDa GelMA‐based baths show slow recovery and mechanical damage caused by the movement of an immersed extrusion nozzle. B) Gelation over time at room temperature of GelMA 90p60 and 160p60. C) 90p60 GelMA rheological properties make it suitable as a suspension bath for embedded extrusion bioprinting. Self‐healing through low (unshaded, 1% strain, 1 Hz) and high (shaded, 500% strain, 1 Hz) strain cycles (n = 3). D) Comparison between μResin and 90p60‐based support baths: qualitative characterization of the optical properties (light scattering) of the microparticle‐based bioresin i) and of the fluid bulk support bath ii). Qualitative characterization via 3D reconstruction and cross‐section visualization of the embedded filaments extruded in the microparticle‐based bioresin iii) and in the fluid bulk support bath iv). E) Quantitative characterization of the circularity of the cross‐section from the embedded filaments (n = 12). F) Printing accuracy of the two formulations, calculated by measuring the filament width as a function of the nozzle diameter and printhead translational velocity (n = 5). G) Angle accuracy and circularity aspect ratio quantification at different printing speeds of the embedded extruded filament in 90p60 fluid bulk support bath (n = 4). H) Multi‐wavelength approach for calibration and printing during EmVP. 520 nm green light, far from the LAP excitation spectrum, is used for the manual alignment of the vial in the Z axis and XY plane, in order to match the position of the extruded features with the initial angle at which the vial will start to rotate and send, in synchrony, the projections of the object to be overprinted. Subsequently, the volumetric printing process starts using a violet‐blue 405 nm laser line. I) Schematic representation of the EmVP process showing the fabrication of a microfluidic chip (channels diameter: 1 mm) i) via bulk bioink extrusion and centimeter‐scale complex structure (channels diameter: 500 µm) ii) via granular bioink extrusion. Scale bars = 4 mm.
Multi‐material Volumetric Bioprinting and Plug‐and‐play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics

February 2025

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128 Reads

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2 Citations

Volumetric Bioprinting (VBP), enables to rapidly build complex, cell‐laden hydrogel constructs for tissue engineering and regenerative medicine. Light‐based tomographic manufacturing enables spatial‐selective polymerization of a bioresin, resulting in higher throughput and resolution than what is achieved using traditional techniques. However, methods for multi‐material printing are needed for broad VBP adoption and applicability. Although converging VBP with extrusion bioprinting in support baths offers a novel, promising solution, further knowledge on the engineering of hydrogels as light‐responsive, volumetrically printable baths is needed. Therefore, this study investigates the tuning of gelatin macromers, in particular leveraging the effect of molecular weight and degree of modification, to overcome these challenges, creating a library of materials for VBP and Embedded extrusion Volumetric Printing (EmVP). Bioresins with tunable printability and mechanical properties are produced, and a novel subset of gelatins and GelMA exhibiting stable shear‐yielding behavior offers a new, single‐component, ready‐to‐use suspension medium for in‐bath printing, which is stable over multiple hours without needing temperature control. As a proof‐of‐concept biological application, bioprinted gels are tested with insulin‐producing pancreatic cell lines for 21 days of culture. Leveraging a multi‐color printer, complex multi‐material and multi‐cellular geometries are produced, enhancing the accessibility of volumetric printing for advanced tissue models.



Multi-material volumetric bioprinting and plug-and-play suspension bath biofabrication via bioresin molecular weight tuning and multiwavelength optics

September 2024

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106 Reads

Volumetric Bioprinting (VBP), enables to rapidly build complex, cell-laden hydrogel constructs for tissue engineering and regenerative medicine. Light-based tomographic manufacturing enables spatial-selective polymerization of a bioresin, resulting in higher throughput and resolution than what achieved using traditional techniques. However, methods for multi-material printing are needed for a broad VBP adoption and applicability. Although converging VBP with extrusion bioprinting in support baths offers a novel, promising solution, further knowledge on the engineering of hydrogels as light-responsive, volumetrically printable baths is needed. Therefore, this study investigates the tuning of gelatin macromers, in particular leveraging the effect of molecular weight and degree of modification, to overcome these challenges, creating a library of materials for VBP and Embedded extrusion Volumetric Printing (EmVP). Bioresins with tunable printability and mechanical properties are produced, and a novel subset of gelatins and GelMA exhibiting stable shear-yielding behavior offers a new, single-component, ready-to-use suspension medium for in-bath printing, which is stable over multiple hours without needing temperature control. As proof-of-concept biological application, bioprinted gels are tested with insulin-producing pancreatic cell lines for 21 days of culture. Leveraging a multi-color printer, complex multi-material and multi-cellular geometries are produced, enhancing the accessibility of volumetric printing for advanced tissue models.

Citations (1)


... Such a system, when effectively combined with wavelengthspecific photocrosslinking or photodegradation chemistries, can allow in situ photo-bioprinting or photodegradation of multimaterial constructs. [51,52] As a conceptual demonstration of synergistic multi-wavelength projection, we activated each laser wavelength in pulses synchronized with the image sequence displayed on the DMD (illustrated in Figure 8A). For instance, the images for the FaSt-Light and ETH logo were divided into three separate images and synchronized with laser activation at 405, 450, or 520 nm, respectively, to create multicolor image projections ( Figure 8B). ...

Reference:

Structured Light Projection Using Image Guide Fibers for In Situ Photo‐biofabrication
Multi‐material Volumetric Bioprinting and Plug‐and‐play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics