Tatsuro Miyake’s research while affiliated with Osaka Dental University and other places

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Publications (31)


Clinical analysis of antibiotic prophylaxis and surgical site infection in third molar surgery
  • Article

December 2024

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1 Read

Journal of Oral and Maxillofacial Surgery Medicine and Pathology

Keita KANO

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Kahori KAWAMURA

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Hideki YOSHIMATSU

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[...]

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Tatsuro MIYAKE


Derivation of the study population from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) cohort
Association between tea types and number of teeth: a cross-sectional study of the Chinese Longitudinal Healthy Longevity Survey
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  • Full-text available

February 2024

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47 Reads

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1 Citation

BMC Public Health

Background Previous studies have suggested that tea consumption may have a positive impact on oral health. However, the effects of different tea types on oral health remain unclear. Therefore, this study aimed to determine the association between residual teeth and consumption habits of different types of tea (green tea, black tea, oolong tea, and scented tea) in older adults. Methods We conducted a secondary analysis using data from the Chinese Longitudinal Healthy Longevity Survey in 2018. In a sample of 6,387 older adults, we performed logistic regression analysis to examine the relationship between persistent tea consumption and oral health according to sex and brushing frequency. The indices for particularly healthy oral health and relative health were set at more than 20 teeth and more than 10 teeth, respectively. Results The study included 2,725 males and 3,662 females, both aged 65 and older. Among individuals with more than 20 teeth, drinking green tea significantly improved oral health in men (adjusted odds ratio [ORs]: 1.377; 95% confidence interval [CI]: 1.082–1.752) and drinking black tea significantly improved the oral health of women (ORs: 2.349, 95%CI: 1.028–5.366). In the daily brushing group, green tea had a significant beneficial effect on increasing the number of teeth in men and black tea had a significant beneficial effect in women. Among individuals with more than 10 teeth, drinking green tea significantly improved oral health in men (ORs: 1.539; 95% CI: 1.209–1.959) and drinking green tea and scented tea significantly improved the oral health of women (ORs: 1.447, 95%CI: 1.052–1.991; ORs: 1.948, 95%CI: 1.137–3.340). In the daily brushing group, consumption of green tea and black tea had significant beneficial effects on increasing the number of teeth in men, whereas that of green tea, black tea, and scented tea had significant beneficial effects in women. Conclusion Long-term green tea consumption in males and black tea consumption in females were significantly associated with maintaining functional dentition (≥20 teeth). Similarly, long-term green tea consumption in males and green tea and scented tea consumption in females were associated with avoiding severe tooth loss (≥10 teeth). Furthermore, in the daily tooth brushing group, long-term consumption of black tea was associated with avoiding severe tooth loss in both sexes. However, tea consumption alone had no effect on oral health without good brushing habits.

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Schedule of nasal vaccination and saliva sampling. Groups of C57BL6/N mice (N = 5 mice) were nasally immunized with HSPs-derived peptide Ags with or without dDA 4 times at weekly intervals for four consecutive weeks. Saliva was collected from the mice in each at 7 days after the final immunization
Salivary SIgA Ab responses to stat23 or prp21 peptide in mice. (A) Salivary SIgA Ab responses to stat23 or prp21 in mice given nasally single Ags with/without dDA. (B) Salivary stat23 or prp21 specific-SIgA Ab in mice given nasally double Ags with/without dDA. The levels of anti-peptide SIgA Abs in saliva were determined by the respective peptide Ag-specific ELISA assay. The values are presented as the median and interquartile range (the first quartile—1.5 IQR, the 3rd quartile + 1.5 IQR) of 15 mice in each group and were compared via two-tailed non-paired Mann–Whitney U-tests. *p < 0.05, **p < 0.01 vs. mice immunized with peptide Ag alone without dDA (dDA (-))
The inhibition assay for binding of live Pg cells to sHAPs incubated with SIgA Ab-enriched saliva from mice administered nasally stat23 with/without dDA (A) or to pHAPs with SIgA Ab-enriched saliva from mice administered nasally prp21 with/without dDA (B). SIgA Ab-enriched saliva (100 µL) (i.e., saliva from which IgM and IgG Abs were removed using affinity columns chromatography) was added to sHAPs (A) or pHAPs (B) in siliconized glass tubes; the resulting mixtures were incubated for 3 h at room temperature. The HAPs were washed three times with 500 µL of KCl buffer. Subsequently, Pg ATCC 33,277 (1 × 10⁸ cells in 200 µL of KCl buffer) was added to each tube. After incubation for another 3 h at room temperature, the HAPs were washed three times with 500 µL of KCl buffer and then transferred to white 96-well plates. The levels of ATP (indicative of live Pg cells bound to the HAPs) were determined using a luciferase assay and luminometer. Values are represented as the means ± SE from three replicates. Comparisons were performed by two-tailed unpaired Student’s t-test. **p < 0.01
Inhibition of the binding of live Pg cells to wsHAPs by SIgA Ab-enriched saliva in wild-type (IgA+/+) or by saliva from IgA−/− mice given nasally both stat23 and prp21 with/without dDA. An aliquot (100 µL) of SIgA Ab-enriched saliva (i.e., saliva from which IgM and IgG Abs had been removed using affinity columns chromatography) or IgA−/− mice saliva samples were added to wsHAPs in the was added to wsHAPs in siliconized glass tubes; the mixtures then was incubated for 3 h at room temperature. Subsequently, the wsHAPs were washed three times with 500 µL of KCl buffer and Pg ATCC 33,277 (1 × 10⁸ cells in 200 µL of KCl buffer) was added to each tube. After incubation for another 3 h at room temperature, the HAPs were washed three times with 500 µl of KCl buffer and then transferred to white 96-well plates. The levels of ATP (indicative of live Pg cells bound to the wsHAPs) were determined using a luciferase assay and luminometer. Values are presented as the mean ± SE from three replicates. Comparisons were performed by two-tailed unpaired Student’s t-test. *p < 0.05
Human salivary protein-derived peptides specific-salivary SIgA antibodies enhanced by nasal double DNA adjuvant in mice play an essential role in preventing Porphyromonas gingivalis colonization: an in-vitro study

February 2023

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40 Reads

BMC Oral Health

Background We previously showed that fimbriae-bore from Poryphyromonas gingivalis (Pg), one of the putative periodontopathogenic bacteria specifically bound to a peptide domain (stat23, prp21) shared on statherin or acidic proline-rich protein 1 (PRP1) molecule of human salivary proteins (HSPs). Here, we investigated whether the nasal administration of DNA plasmid expressing Flt3 ligand (pFL) and CpG oligodeoxynucleotide 1826 as double DNA adjuvant (dDA) with stat23 and prpr21 induces antigen (Ag)-specific salivary secretory IgA (SIgA) antibodies (Abs) in mice. Further, we examined that stat23- and prpr21-specific salivary SIgA Abs induced by dDA have an impact on Pg-binding to human whole saliva-coated hydroxyapatite beads (wsHAPs). Material and methods C57BL/6N mice were nasally immunized with dDA plus sta23 or/and prp21 peptide as Ag four times at weekly intervals. Saliva was collected one week after the final immunization and was subjected to Ag-specific ELISA. To examine the functional applicability of Ag-specific SIgA Abs, SIgA-enriched saliva samples were subjected to Pg binding inhibition assay to wsHAPs. Results Significantly elevated levels of salivary SIgA Ab to stat23 or prp21 were seen in mice given nasal stat23 or prp21 with dDA compared to those in mice given Ag alone. Of interest, mice nasally given the mixture of stat23 and prp21 as double Ags plus dDA, resulted in both stat23- and prp21-specific salivary SIgA Ab responses, which are mediated through significantly increased numbers of CD11c⁺ dendritic cell populations and markedly elevated Th1 and Th2 cytokines production by CD4⁺ T cells in the mucosal inductive and effector tissues. The SIgA Ab-enriched saliva showed significantly reduced numbers of live Pg cells binding to wsHAPs as compared with those in mice given double Ags without dDA or naïve mice. Additionally, saliva from IgA-deficient mice given nasal double Ags plus dDA indicated no decrease of live Pg binding to wsHAPs. Conclusion These findings show that HSP-derived peptides-specific salivary SIgA Abs induced by nasal administration of stat23 and prp21 peptides plus dDA, play an essential role in preventing Pg attachment and colonization on the surface of teeth, suggesting a potency that the SIgA may interrupt and mask fimbriae-binding domains in HSPs on the teeth.


Fig. 2 -Local indicator of spatial autocorrelation (LISA) cluster map for accessibility index rate (AIR) at the geographic level in Japanese municipalities.T a g g e d E n d
Descriptive statistics of caries prevalence adjusted using Bayes estimation and decayed and filled teeth (dft) by the number of municipalities.
GIS-Based Study of Dental Accessibility and Caries in 3-Year-Old Japanese Children

December 2022

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39 Reads

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1 Citation

International Dental Journal

Objective This study aimed to reveal the geographic accessibility of dental clinics for most municipalities in Japan in 2015 and to explore the association between dental accessibility and dental caries status in 3-year-old children. Methods We computed the accessibility index and accessibility index rate for the population outside a 1-km radius of dental clinics using a geographic information system. We also used spatial autocorrelation analysis (Moran's I statistic) to examine the spatial clustering patterns of dental accessibility in Japanese municipalities. In addition, we adjusted the prevalence of dental caries for most municipalities using empirical Bayesian estimation. Finally, we applied multiple linear regression to scrutinise the associations between dental caries status, including the prevalence of dental caries and decayed and filled teeth (dft), and dental accessibility, with adjustments made for other sociodemographic variables. Results The distribution of dental accessibility in Japanese municipalities is relatively unequal. Dental accessibility is decent in the 3 metropolitan areas around Tokyo, Osaka, and Nagoya but poor in the Tohoku and Kyushu regions. In addition, dental accessibility is significantly related to the prevalence of dental caries and dft after adjusting for other sociodemographic variables (P < .005). Conclusions This study suggests that dental accessibility is considerably connected to the dental caries status of 3-year-old children after excluding financial burden. Preschool children in areas with poor dental accessibility are likely to have poor dental caries status. We also verified the inequality of dental accessibility amongst Japanese municipalities. For the future development of primary oral health care, more attention should be paid to people with a disadvantage in terms of dental accessibility.


Figure 2 Cumulative incidence of second primary cancers (SPCs) by site of oral and pharyngeal cancer.
Second primary cancers (SPCs) by site of oral and pharyngeal cancer (including SPCs < 6 months after initial cancer diagnosis).
Incidence of second primary cancers in oral and pharyngeal cancer patients using a large medical claims database in Japan

December 2022

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21 Reads

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1 Citation

Journal of Dental Sciences

Background/purpose As the occurrence of second primary cancers (SPCs) is strongly related to the survival rate of patients with oral and pharyngeal cancers, early detection and treatment are important. Therefore, this study aimed to clarify the incidence of SPCs and their risk factors in patients with oral and pharyngeal cancer. Materials and methods This observational study was conducted using data from the administrative claims database of 21,736 participants with oral and pharyngeal cancer from January 2005 to December 2020. We evaluated the cumulative incidence of SPCs among patients with oral and pharyngeal cancers using the Kaplan–Meier method. The Cox proportional-hazard model was used for multivariate analysis. Results Of the 1633 patients with oral and pharyngeal cancer who qualified for analysis, 388 developed SPCs (incidence rate, 7.994/1000 person-months). The multivariate analysis showed that the risk of developing SPCs was affected by age at diagnosis of oral and pharyngeal cancer, cancer treatment, and anatomical site of the primary cancer. Conclusion Patients with oral and pharyngeal cancers are at a high risk of developing SPCs. The data from this study may be useful in providing accurate information to patients with oral and oropharyngeal cancer.


Study flow diagram
Variables in the propensity score model
Dental and medical utilization and cost between smokers and non-smokers
Association between smoking habits and dental care utilization and cost using administrative claims database and specific medical check-up data

September 2022

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20 Reads

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2 Citations

BMC Oral Health

Background This study aims to evaluate the association between smoking habits and dental care utilization and cost in individuals registered with the Japan Health Insurance Association, Osaka branch. Methods We used the administrative claims database and specific medical check-up data and included 226,359 participants, who visited dental institutions, underwent dental examinations, and underwent specific medical checkups, with smoking data from April 2016 to March 2017. We calculated propensity scores with age, gender, exercise, eating habits, alcohol intake, and sleep. We also compared dental care utilization with the total cost of each procedure. Results According to propensity score matching, 62,692 participants were selected for each group. Compared to non-smokers, smokers were younger, and a higher proportion were men. Smokers tended to skip breakfast, have dinner just before bed, and drink alcohol. After adjusting for potential confounding factors with propensity score matching, the mean annual dental cost among smokers was significantly higher than non-smokers. The prevalence of pulpitis, missing teeth, and apical periodontitis were higher among smokers than non-smokers, while inlay detachment, caries, and dentine hypersensitivity were higher among non-smokers. Conclusion This study suggests that smokers have higher dental cost consisted of progressive dental caries, missing teeth, and uncontrolled acute inflammation that necessitated the use of medications. It is suggested that smokers tend to visit the dentist after their symptoms become severe.


Clinical parameters and applicable of metabolic syndrome
Association between smoking habits and dental care utilization and cost using administrative claims database and specific medical check-up data

April 2022

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7 Reads

Background This study aims to evaluate the association between smoking habits and dental care utilization and cost in individuals registered with the Japan Health Insurance Association, Osaka branch. Methods We used the administrative claims database and specific medical check-up data included 226,368 participants who visited dental institutions, underwent dental examination and underwent specific medical check-up with smoking data from April 2016 to March 2017. We calculated propensity scores with age, gender, exercise, eating habits, alcohol intake, and sleep. According to propensity score matching, 62,573 participants were selected for each group. We also compared dental care utilization with the total cost of each procedure. Results Compared to non-smokers, smokers were younger, with a higher proportion of males. Smokers tended to skip breakfast, have dinner just before bed, and drink alcohol. After adjustment for potential confounding factors with propensity score matching, the median annual dental costs (38,790 (JPY) (Interquartile range (IQR): 19,220 − 71,065)) were significantly higher among smokers than non-smokers (34,530 (JPY) (IQR: 17,960 − 61,420)) (P < 0.001). We calculated the total number of disease codes, and differences between smokers and non-smokers Pulpitis, missing teeth, and periapical periodontitis were coded in smokers, while inlay detachment, caries, and dentine hypersensitivity were coded in non-smokers. Conclusion The annual dental cost was significantly higher among smokers than non-smokers. The disease codes in smokers consisted of progressive dental caries, missing teeth, and uncontrolled acute inflammation that necessitated the use of medications. It is suggested that smokers tend to visit the dentist after their symptoms become severe.


A nasal double DNA adjuvant system induces atheroprotective IgM antibodies via dendritic cell-B-1a B cell interactions

January 2022

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21 Reads

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5 Citations

Vaccine

We previously demonstrated that the dendritic cell (DC)-targeting nasal double DNA adjuvant system, which consists of a DNA plasmid expressing Flt3 ligand (pFL) and CpG oligodeoxynucleotide 1826 (CpG ODN), elicits specific immune responses to various antigens in the mucosal and systemic compartments. Here, we investigated, using phosphorylcholine (PC)-conjugated keyhole limpet hemocyanin (PC-KLH) as an antigen, whether the nasal double DNA adjuvant system induces protective immunity to atherosclerosis in apolipoprotein E-deficient (ApoE KO) mice. Further, we assessed the molecular and cellular mechanisms in the induction of anti-PC-specific immune responses. Nasal immunization with PC-KLH plus pFL and CpG ODN enhanced induction of PC-specific IgM in plasma, peritoneal fluids, and nasal washes when compared with mice administered PC-KLH alone. Of importance, these antibodies exhibited highly specific binding to the PC molecule, and dose-dependent binding to anti-T15 idiotype (AB1-2). Twelve weeks after the last immunization, the nasal double DNA adjuvant system with PC-KLH resulted in a reduction of atherogenesis in the aortic arch of ApoE KO mice. Therefore, we next assessed immunocytological mechanism to induce these antibodies. The nasal double DNA adjuvant system with PC-KLH resulted not only in significantly increased frequencies of CD11c⁺ DCs in the spleen, peritoneal cavity (PEC), and nasopharyngeal-associated lymphoid tissues (NALT), but also significantly increased expression of a proliferation-inducing ligand and B-cell-activating factor by CD11c⁺ DCs. In addition, the double DNA adjuvant system induced significantly increased numbers of B-1 B cells in the spleen, PEC, and NALT, and increased expression of transmembrane activator and calcium modulator and cyclophilin ligand interactor on CD5⁺ B220⁺ (B-1a) B cells. These findings demonstrated that the nasal double DNA adjuvant system with PC-KLH resulted in the induction of T15-like antibodies in the mucosal and systemic lymphoid tissues through interaction between DCs and B-1a B cells, and inhibited the progression of atherogenesis.


Citations (11)


... The risk for the development of second primary malignant neoplasms (SPMN) is estimated to be 3-7% per year, with a 20-year cumulative rate of 36% [91,92]. Other factors that are considered when determining follow-up intervals include alcohol/tobacco status at diagnosis, with the higher recurrence rates occurring in those with a combination of smoking and alcohol abuse (49%), followed by smoking alone or alcohol alone (13% each), tumor size, and histopathological grade [88,93]. ...

Reference:

Oral Complications from Oropharyngeal Cancer Therapy
Incidence of second primary cancers in oral and pharyngeal cancer patients using a large medical claims database in Japan

Journal of Dental Sciences

... Regarding this concern, a recent study demonstrated that the market-driven location of dental clinics causes an imbalance in the distribution of dental and medical resources between large and small municipalities, resulting in better dental accessibility in Japanese municipalities with larger populations than in municipalities with smaller populations. 34 In China, various studies have shown that there is still a persistent unfair distribution of medical resources. The fairness of medical resources in each province is generally low due to the different economic development levels in eastern, central, and western China as well as the imbalance of urban and rural development. ...

GIS-Based Study of Dental Accessibility and Caries in 3-Year-Old Japanese Children

International Dental Journal

... Hasonló eredményeket tudomásunk szerint sem a hazai, sem a nemzetközi szakirodalomban nem közöltek. Vizsgálataink lényeges korlátja, hogy a teszthelyeken megjelenő betegektől az anamnézis felvétele során nem gyűjtöttünk adatokat sem az esetleges SARS-CoV-2 okozta megbetegedésük, sem a felvett oltások, sem az eredményeket feltételezhetően tovább módosító dohányzás [38] tekintetében. ...

Association between smoking habits and dental care utilization and cost using administrative claims database and specific medical check-up data

BMC Oral Health

... This induced antibodies against OxLDL, but also against PC and ameliorated atherosclerosis significantly [120]. In a recent paper, a nasal double DNA adjuvant system with PC-KLH induced IgM anti-PC and reduction in aortic atherosclerosis in apoE k/o mice [121]. Additionally, passive immunization by IgM anti-PC reduced atherosclerosis in apoE ko mice using a venous graft model and weekly intraperitoneal injections of anti-PC. ...

A nasal double DNA adjuvant system induces atheroprotective IgM antibodies via dendritic cell-B-1a B cell interactions
  • Citing Article
  • January 2022

Vaccine

... Bronchoalveolar lavage fluid (BALF) was collected using 2 ml of PBS as described previously (22). Nasal wash was collected using 1 mL of PBS as described (23). Concentrations of anti-NP IgA or IgG1 in sera, BALF, or nasal wash were determined by ELISA using NP 13.6 -BSA as a coating antigen as described previously (24). ...

Respiratory FimA-Specific Secretory IgA Antibodies Upregulated by DC-Targeting Nasal Double DNA Adjuvant Are Essential for Elimination of Porphyromonas gingivalis

... Pre-emptive analgesia involves analgesic medication being administered prior to tissue injury, in other words before the reception, transmission, modulation, and nociception of the aggressive stimulus, the objective being to prevent hyperalgesia and the resulting pain-amplifying stimulus 11 . Pre-emptive analgesia has been used as an effective pain control method in pediatric surgeries, and acetaminophen is one of the most commonly used drugs for pre-emptive analgesia in pediatric patients 7,9,[12][13][14] . ...

Effects of preemptive analgesia with intravenous acetaminophen on postoperative pain relief in patients undergoing third molar surgery: A prospective, single-blind, randomized controlled trial

Medicina Oral Patología Oral y Cirugia Bucal

... Vaccines 2024, 12, 619 2 of 17 cavity, many studies focus on mucosal immune pathways [12][13][14], highlighting mucosal immunity as a promising approach for preventing Pg infections. This review delves into the three main factors (antigens, adjuvants, and immunization routes) involved in developing Pg vaccines, outlining the challenges encountered in research. ...

Nasal double DNA adjuvant induces salivary FimA-specific secretory IgA antibodies in young and aging mice and blocks Porphyromonas gingivalis binding to a salivary protein

BMC Oral Health

... In this regard, we have shown that a DNA plasmid expressing the Flt3 ligand (pFL) as a mucosal adjuvant preferentially increases CD8 + dendritic cells (DCs) in the mucosal tissues and subsequently elicits Ag-specific Th2-type mucosal immune responses mediated by IL-4, inducing the production of CD4 + T cells when mice were nasally immunized with a combination of ovalbumin (OVA) or bacterial Ag plus pFL [14,15]. Furthermore, we have shown that nasal administration of a double DNA adjuvant (dDA) consisting of CpG oligodeoxynucleotide 1826 (CpG ODN), a known inducer of Th1-type immune responses, in combination with pFL, elicits mucosal and systemic antibody (Ab) responses to OVA [16], pneumococcal surface protein A (PspA) [17], influenza virus hemagglutinin [18], and recombinant FimA (rFimA) [19], through DCs activation [20]. In addition, we previously have shown that nasal administration of rFimA, in combination with dDA, effectively reduced Pg infection in the respiratory tract; this efficacy was mediated by increased numbers of CD8 + and CD11b + DCs and balanced Th1-and Th2-type cytokine responses in the mucosal tissues [21]. ...

Dendritic cell-targeting DNA-based nasal adjuvants for protective mucosal immunity to Streptococcus pneumoniae: DNA adjuvants for potent mucosal immunity
  • Citing Article
  • May 2017

Microbiology and Immunology

... To address this issue, various surface modifications are applied to Ti and its alloys to improve their biological activity. At present, these modifications include plasma spraying, ion implantation, chemical deposition, and micro-arc oxidation technology, which enhance the bioactivity of Ti by changing the chemical composition, roughness, and morphology of its surface [11][12][13][14][15][16][17]. Among these methods, treatment with a strong alkali solution has been widely investigated because of its operational convenience, practicality, and effectiveness [18,19]. ...

Bioactivity of Titanium Surface Nanostructures Following Chemical Processing and Heat Treatment

Journal of Hard Tissue Biology

... Non-synonymous SNPs (ns SNPs) are present in coding region of genome which is frequently leads to alteration in amino acid residues of gene product. This single lost or additional nucleotide causes a frame shift mutation (30) . This effect changes the protein that is expressed, this possibly will cause a harmful mutation (31) . ...

Single Nucleotide Polymorphisms (SNPs) associated with tooth loss
  • Citing Conference Paper
  • April 2009