Tatiana Thouroude's research while affiliated with University of Angers and other places

Publications (48)

Article
Full-text available
Nudix hydrolases are conserved enzymes ubiquitously present in all kingdoms of life. Recent research revealed that several Nudix hydrolases are involved in terpenoid metabolism in plants. In modern roses, RhNUDX1 is responsible for formation of geraniol, a major compound of rose scent. Nevertheless, this compound is produced by monoterpene synthase...
Article
Full-text available
Blooming seasonality is an important trait in ornamental plants and was selected by humans. Wild roses flower only in spring whereas most cultivated modern roses can flower continuously. This trait is explained by a mutation of a floral repressor gene, RoKSN, a TFL1 homologue. In this work, we studied the origin, the diversity and the selection of...
Article
Full-text available
Prickles are common structures in plants that play a key role in defense against herbivores. In the Rosa genus, prickles are widely present with great diversity in terms of form and density. For cut rose production, prickles represent an important issue, as they can damage the flower and injure workers. Our objectives were to precisely describe the...
Article
Reticulation, caused by hybridization and allopolyploidization, is considered an important and frequent phenomenon in the evolution of numerous plant lineages. Although both processes represent important driving forces of evolution, they are mostly ignored in phylogenetic studies involving a large number of species. Indeed only a scattering of meth...
Article
Full-text available
Key message: Two environmentally stable QTLs linked to black spot disease resistance in the Rosa wichurana genetic background were detected, in different connected populations, on linkage groups 3 and 5. Co-localization between R-genes and defense response genes was revealed via meta-analysis. The widespread rose black spot disease (BSD) caused by...
Article
Full-text available
Key message: The genetic determinism of prickle in rose is complex, with a major locus on LG3 that controls the absence/presence of prickles on the rose stem. Rose is one of the major ornamental plants. The selection of glabrous cultivars is an important breeding target but remains a difficult task due to our limited genetic knowledge. Our objecti...
Article
Roses use a non‐canonical pathway involving a Nudix hydrolase, RhNUDX1 , to synthesize their monoterpenes, especially geraniol. Here we report the characterization of another expressed NUDX1 gene from the rose cultivar Rosa x wichurana , RwNUDX1‐2 . In order to study the function of the RwNUDX1‐2 protein, we analysed the volatile profiles of an F1‐...
Article
Black spot disease (BSD), caused by the fungus Diplocarpon rosae Wolf, is one of the most important and widespread disease of roses in outdoor landscaping. Up to now, an efficient control of black spot disease still requires intensive use of fungicides. However, new laws to decrease agrochemical use (Labbé, 2014) have encouraged breeders and resear...
Article
The haploid ascomycete Diplocarpon rosae Wolf is the causal agent of black spot disease on roses, a widespread and devastating disease in the outdoor landscape. In this study, we established an Eurasian collection of 77 monoconidial strains of D. rosae: 50 strains collected on cultivated roses in Europe and Asia, and 27 strains on wild roses in Kaz...
Article
Rose is one of the most successful plants in the ornamental sector for gardens, cutting flowers and flowerpots. This tremendous success can be partially explained by selection of roses that can flower continuously. Rose, genus Rosa, is a good model to study seasonality of blooming, a developmental process which is poorly understood. We have previou...
Article
The rose is the queen of flowers and is widely used as a garden plant and for the cut flower market. Roses are also used for the production of essential oil for the cosmetic and perfume industries. A lot of botanical roses are scented and use their volatiles to attract pollinators. Fragrances in garden roses are very diverse and scent has always be...
Article
Black spot (caused by the fungal pathogen Diplocarpon rosae) is the most severe disease of roses in the outdoor landscape. Most cultivars are susceptible to this disease and its control requires repeated fungicide treatments. New rules on pesticide use encourage breeders to develop roses with a high resistance level to black spot. Growing genetical...
Article
Full-text available
Floral scent is one of the most important characters in horticultural plants. Roses (Rosa spp.) have been cultivated for their scent since antiquity. However, probably by selecting for cultivars with long vase life, breeders have lost the fragrant character in many modern roses, especially the ones bred for the cut flower market. The genetic inheri...
Article
Full-text available
Rose is the world's most important ornamental plant, with economic, cultural and symbolic value. Roses are cultivated worldwide and sold as garden roses, cut flowers and potted plants. Roses are outbred and can have various ploidy levels. Our objectives were to develop a high-quality reference genome sequence for the genus Rosa by sequencing a doub...
Preprint
Rose is the world’s most important ornamental plant with economic, cultural and symbolic value. Roses are cultivated worldwide and sold as garden roses, cut flowers and potted plants. Rose has a complex genome with high heterozygosity and various ploidy levels. Our objectives were ( i ) to develop the first high-quality reference genome sequence fo...
Article
Full-text available
Hybridization with introduced genetic resources is commonly practiced in ornamental plant breeding to introgress desired traits. The 19th century was a golden age for rose breeding in France. The objective here was to study the evolution of rose genetic diversity over this period, which included the introduction of Asian genotypes into Europe. A la...
Article
In this paper, we develop a statistical methodology applied to the characterization of flowering curves using Gaussian mixture models. Our study relies on a set of rosebushes flowering data, and Gaussian mixture models are mainly used to quantify the reblooming behavior of each one. In this regard, we also suggest our own selection criterion to tak...
Article
Rose is the ornamental species with the highest financial impact. Floral traits such as the number of petals and the date of flowering are major characteristics of ornamental plants. Our objective is to study the genetic determinism of floral traits: date of flowering and number of petals, which are a major issue for rose breeders. The study was co...
Article
Understanding the genetic basis of plant architecture is limited for woody plants due to the challenges of assessing the inheritance of their complex architecture. We aimed to evaluate the genetic variability of plant form and stature in a garden rose population, analyse the inheritance of plant architecture and its linkage with flowering behaviour...
Article
In rose, RoKSN, a TFL1 homologue, is a key regulator of continuous flowering. To study the function of this gene in planta, protocols of plant transformation are needed. We complemented tfl1 Arabidopsis mutants and ectopically expressed RoKSN in a continuous-flowering rose. In Arabidopsis, RoKSN complemented the tfl1 mutant by rescuing late floweri...
Article
FT/TFL1 family members have been known to be involved in the development and flowering in plants. In rose, RoKSN, a TFL1 homologue, is a key regulator of flowering, whose absence causes continuous flowering. Our objectives are to functionally validate RoKSN and to explore its mode of action in rose. We complemented Arabidopsis tfl1 mutants and ecto...
Article
The Quantitative Trait Loci (QTL) mapping of plant architecture is a critical step for understanding the genetic determinism of plant architecture. Previous studies adopted simple measurements, such as plant-height, stem-diameter and branching-intensity for QTL mapping of plant architecture. Many of these quantitative traits were generally correlat...
Article
Light and temperature are two environmental factors that deeply affect bud outgrowth. However, little is known about their impact on the bud burst gradient along a stem and their interactions with the molecular mechanisms of bud burst control. We investigated this question in two acrotonic rose cultivars. We demonstrated that the darkening of dista...
Book
In rose, RoKSN, a TFL1 homologue, is a key regulator of continuous flowering. To study the function of this gene in planta, protocols of plant transformation are needed. We complemented tfl1 Arabidopsis mutants and ectopically expressed RoKSN in a continuous-flowering rose. In Arabidopsis, RoKSN complemented the tfl1 mutant by rescuing late floweri...
Article
Full-text available
The role of gibberellins (GAs) during floral induction has been widely studied in the annual plant Arabidopsis thaliana. Less is known about this control in perennials. It is thought that GA is a major regulator of flowering in rose. In spring, low GA content may be necessary for floral initiation. GA inhibited flowering in once-flowering roses, wh...
Data
Genes differentially expressed during early floral organogenesis in R. chinensis cv. Old Blush. (XLSX)
Data
Genes differentially expressed during flower opening and senescence in R. chinensis cv. Old Blush. (XLSX)
Data
Genes differentially expressed during floral initiation in R. wichurana . (XLSX)
Data
Genes differentially expressed during floral initiation in R. x hybrida cv. Félicité et Perpétue. (XLSX)
Data
Real time quantitative RT-PCR (qPCR) analysis of 18 selected differentially expressed genes during rose floral organogenesis and senescence in R. chinensis cv Old Blush. (TIFF)
Data
List of genes repressed (A) or activated (B) during flower initiation. (XLSX)
Data
Microarray and qRT-PCR results of 25 selected genes with their replicate-level Pearson correlation. (DOCX)
Data
Full array sequences annotation and ontology. (XLSX)
Data
Genes differentially expressed during floral organ elongation in R. chinensis cv. Old Blush. (XLSX)
Article
Full-text available
Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and f...
Article
Flowering is a key event in plant life, and is finely tuned by environmental and endogenous signals to adapt to different environments. In horticulture, continuous flowering (CF) is a popular trait introduced in a wide range of cultivated varieties. It played an essential role in the tremendous success of modern roses and woodland strawberries in g...
Article
Full-text available
The pattern of development of the inflorescence is an important characteristic in ornamental plants, where the economic value is in the flower. The genetic determinism of inflorescence architecture is poorly understood, especially in woody perennial plants with long life cycles. Our objective was to study the genetic determinism of this characteris...
Article
Exhaustive studies on flowering control in annual plants have provided a framework for exploring this process in other plant species, especially in perennials for which little molecular data are currently available. Rose is a woody perennial plant with a particular flowering strategy--recurrent blooming, which is controlled by a recessive locus (RB...
Conference Paper
The transition from vegetative growth to flowering is a key event in plant life, and is finely regulated by endogenous and environmental signals. In rose, the ability to flower continuously represents an important economical and ornamental trait. The continuous flowering (CF) phenotype is due to a mutation of a TFL1 homologue, RoKSN. The insertion...

Citations

... Rosa based on morphological characteristics (Joly and Brueau 2007;Lewis et al. 2015) and resolved as paraphyletic with the other species in R. sect. Rosa based on molecular evidence (Debray et al. 2019 and2021). ...
... In addition, the null allele of RoKSN, RoKSN null , and the allele RoKSN LTR , which led to occasional re-blooming of the climbing rose mutants, were also found [26,31]. Recently, a new allele, RoKSN A181 , was identified [32]. Roses with the RoKSN A181 allele, which has significantly less expression of RoKSN than OF roses, could rebloom and did not possess both alleles of RoKSN copia or RoKSN LTR [32]. ...
... This has been recently done for MLO genes in various species, including soybean (Deshmukh et al. 2014), Cicer arietinum and Medicago truncatula (Deshmukh et al. 2017), Populus trichocarpa (Filiz and Vatansever 2018), pumpkin (Win et al. 2018), lentil (Polanco et al. 2018), bitter gourd (Chen et al. 2021), for the promotor region of MLO genes in melon and other species (Andolfo et al. 2019), and for other susceptibility genes (Pirrello et al. 2021). In rose overviews have been generated for WRKY genes , NBS-LRR genes (Lopez Arias et al. 2020), the disease resistance gene family Rdr1 (Menz et al. 2020), AP2/ERF transcription factors (Li et al. 2021), and S-RNases and F-box genes involved in self-incompatibility (Vieira et al. 2021). These inventories may be used as a basis to find or generate loss-of-function alleles of relevant MLO genes. ...
... Fig. S26, S27). QTL2 and QTL3 were not identified previously in genetic analyses with F1 populations [14,32], suggesting that populations in different generations might provide different powers in genetic analyses. ...
... Rosa (Roccia et al., 2019;Spiller et al., 2010;Sun et al., 2020), some important sites for regulating floral scent were discovered using QTL mapping, but measuring floral scent of hundreds of accessions by GC-MS is time-consuming and expensive. Rosa also includes floral fragrance analysis based on sensory analysis and GWAS (Schulz et al., 2021), although the accuracy of this fragrance detection method still needs to be improved. ...
... BSD was visually scored for each population two years after planting them in the field and at the peak of disease (between July and September depending on the year). BSD was scored on the lower part of the plant using a visual rating scale from 0 to 5 presented in Supplementary Fig. 1 (Marolleau et al. 2020;Soufflet-Freslon et al. 2019). These scores correspond to a percentage of infected leaves and a degree of defoliation (scores 4 and 5). ...
... This map length is considerably shorter than the integrated consensus map (573.66 cM) generated by Bourke et al. (2017). Despite the difference in length the integrated consensus map generated in this study is thought to be of high quality as the weighted root mean square error for each linkage group was low (Supplementary Material 2), the markers covered most of the physical space for the two R. chinensis assemblies, and a high level of collinearity was observed between the map and each R. chinensis physical assembly (Supplementary Tables S1-S3; Hibrand Saint-Oyant et al., 2018;Raymond et al., 2018). The weighted root mean square error describes the estimated error when modeling the pairwise estimates of recombination frequency versus the multi-point estimate of the recombination frequency. ...
... Indeed, genetic resistance is a critical challenge to successful disease management in an environmentally friendly and cost-effective manner. Researchers have studied the genetic resistance to BSD, helping to identify modern rose varieties and wild species with high levels of resistance (Wiggers et al. 1997;Carlson-Nilsson 2001;Boontiang 2003;Blechert and Debener 2005;Uggla and Carlson-Nilsson 2005;Harp et al. 2009;Schulz et al. 2009;Soufflet-Freslon et al. 2019). However, breeding for BSD resistance is complex due to the development of new pathogenic races and different ploidy levels in the genus Rosa Uggla and Carlson-Nilsson 2005;Whitaker et al. 2007;Debener 2017). ...
... Rosa (Roccia et al., 2019;Spiller et al., 2010;Sun et al., 2020), some important sites for regulating floral scent were discovered using QTL mapping, but measuring floral scent of hundreds of accessions by GC-MS is time-consuming and expensive. Rosa also includes floral fragrance analysis based on sensory analysis and GWAS (Schulz et al., 2021), although the accuracy of this fragrance detection method still needs to be improved. ...
... Therefore, detection and analysis of floral scent characteristics in genetic populations is crucial for crop molecular breeding. Other important ornamental characteristics of flowers, such as flower color (Zhou et al., 2022;Chen et al., 2021;Cheng et al., 2021;Hibrand et al., 2018;Yang et al., 2020a), flower type (Chong et al., 2019;Hibrand et al., 2018;Song et al., 2020), and disease (Fu et al., 2017a;Mekapogu et al., 2021;Zhou et al., 2021a), have been extensively studied genetically, but there have been few studies on the detection and analysis of floral scent characteristics in genetic populations. ...