Tatiana I. Gromovykh's research while affiliated with Moscow Witte University and other places

Publications (7)

Article
This research is dedicated to the studies of the microscale morphology of bacterial cellulose (BC) obtained by means of static cultivation of Gluconacetobacter hansenii GH-1/2008. We found that the microscale morphology depended on the BC production rate that was varied by using different glucose concentrations in the cultivation medium. It was rev...
Article
The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task. The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-frien...
Article
Bacterial cellulose (BC) is a natural polymer that has a number of unique properties that determine the need to synthesize large amounts of it and to search the ways to increase the productivity of strains and to optimize the nutritive media. It is known that the choice of the producer for BC synthesis has an impact on its final properties and on t...
Article
In this paper, we perform a systematic analysis of the structural organization of bacterial cellulose (BC). We report four types of organization of the BC mass, produced by Gluconacetobacter hansenii that occur depending on cultivation conditions. Two of those, particularly, plywood type one and layers of micro-sized tubes were observed and describ...
Article
In this paper, we report our attempts to elaborate the method of purification and simultaneous sterilization of bacterial cellulose films by means of processing them under high pressure, including supercritical conditions for carbon dioxide, for future application in medicine. Bacterial cellulose films were produced by Gluconacetobacter hansenii. T...
Article
Full-text available
The cytotoxic and antitumor effect of liposomal form of silibinin was studied. It was shown that the liposomal silibinin reveals more pronounced cytotoxic activity in vitro against HeLa (cervix carcinoma), HT1080 (fibrosarcoma), and MCF-7 (breast adenocarcinoma) human tumor cell lines compared with non-liposomal silibinin. In vivo experiments demon...
Article
Full-text available
The liposomal form of silibinin was obtained, and its antihepatotoxic activity in mice was studied using a model of acute toxic hepatitis caused by injection of carbon tetrachloride or paracetamol. It was shown that the use of the drug in therapy or prevention regimens leads to normalization of levels of transaminases and total protein in the blood...

Citations

... Most of the research published so far is dedicated to the best-studied strain Acetobacter xylinus that is believed to have the highest productivity (Hassan et al., 2015;Lee et al., 2015;Mohammadkazemi et al., 2015;Tabaii & Emtiazi, 2016;Wang et al., 2018). Still, there are several publications covering G. hansenii cultivation on HS medium (Park et al., 2003), modified HS media (Hutchens et al., 2007;Kiselyova et al., 2021;Rani & Appaiah, 2011), corn steep liquor (Costa et al., 2017), coffee cherry husk (Rani & Apaiah, 2013), waste beer yeast (Lin et al., 2014), frutose (Lazarini et al., 2018), polysaccharide fermentation wastewater (Zhao et al., 2018), sisal juice (Lima et al., 2017), xylose (Ishihara et al., 2002). Meanwhile, most of authors compare different carbon sources rather than the concentration of the carbon source for a given strain (Hutchens et al., 2007), or even vary both (Mohammadkazemi et al., 2015). ...
... In this paper, we study the impact of BC cultivation conditions, particularly the concentration of carbon source, on the microscale morphology and the mechanical properties of the BC mass obtained by static condition fermentation of Gluconacetobacter hansenii. Our previous studies (Gromovykh et al., 2020) indicate that the formation of layered structures is mediated by the rate of BC synthesis and accumulation. Therefore, in the present work, our main goal was to find further proof of this hypothesis and to reveal the correlation between the BC films structure and the productivity of G. hansenii under the given cultivation conditions, since the latter is proportional to the average accumulation rate, provided the incubation time is the same. ...
... Researchers point out that supercritical CO 2 processing and treatment with carbonic acid under high pressure are sufficient purification procedures for microbial cellulose that will be used in biomedical applications. Recent experiments indicate that treatment in biphasic systems is more effective in terms of maintaining the main structure of the cellulose network [59]. ...
... Nowadays, large amounts of silibinin powders can be easily prepared. However, recent reports have demonstrated that silibinin can also adopt conformations that are more complex such as liposomal forms [23] and emulsomes [24]. Furthermore, it can be confined in oil nanocapsules [25], adsorbed on magnetic nanoparticles [26], and form silibinin-metal ion complexes with interesting properties [27]. ...