Tatiana I. Gromovykh's research while affiliated with Moscow Witte University and other places

Publications (9)

Article
Full-text available
New functional medical materials with antibacterial activity based on biocompatible bacterial cellulose (BC) and Ag nanoparticles (Ag NPs) were obtained. Bacterial cellulose films were prepared by stationary liquid-phase cultivation of the Gluconacetobacter hansenii strain GH-1/2008 in Hestrin–Schramm medium with glucose as a carbon source. To func...
Article
This research is dedicated to the studies of the microscale morphology of bacterial cellulose (BC) obtained by means of static cultivation of Gluconacetobacter hansenii GH-1/2008. We found that the microscale morphology depended on the BC production rate that was varied by using different glucose concentrations in the cultivation medium. It was rev...
Article
Due to unique natural characteristics such as biocompatibility, nontoxicity, mechanical stability, and high moisture content, bacterial cellulose can be efficiently used for the production of new medical materials, including various wound dressings. Bacterial cellulose can be produced by bacteria of the genera Gluconaceto-bacter, Aerobacter, Rhizob...
Article
The functionalization of the bacterial cellulose (BC) surface with a chitosan biopolymer to expand the areas of possible applications of the modified BC is an important scientific task. The creation of such composites in the carbonic acid solutions that were performed in this work has several advantages in terms of being biocompatible and eco-frien...
Article
Bacterial cellulose (BC) is a natural polymer that has a number of unique properties that determine the need to synthesize large amounts of it and to search the ways to increase the productivity of strains and to optimize the nutritive media. It is known that the choice of the producer for BC synthesis has an impact on its final properties and on t...
Article
In this paper, we perform a systematic analysis of the structural organization of bacterial cellulose (BC). We report four types of organization of the BC mass, produced by Gluconacetobacter hansenii that occur depending on cultivation conditions. Two of those, particularly, plywood type one and layers of micro-sized tubes were observed and describ...
Article
In this paper, we report our attempts to elaborate the method of purification and simultaneous sterilization of bacterial cellulose films by means of processing them under high pressure, including supercritical conditions for carbon dioxide, for future application in medicine. Bacterial cellulose films were produced by Gluconacetobacter hansenii. T...
Article
Full-text available
The cytotoxic and antitumor effect of liposomal form of silibinin was studied. It was shown that the liposomal silibinin reveals more pronounced cytotoxic activity in vitro against HeLa (cervix carcinoma), HT1080 (fibrosarcoma), and MCF-7 (breast adenocarcinoma) human tumor cell lines compared with non-liposomal silibinin. In vivo experiments demon...
Article
Full-text available
The liposomal form of silibinin was obtained, and its antihepatotoxic activity in mice was studied using a model of acute toxic hepatitis caused by injection of carbon tetrachloride or paracetamol. It was shown that the use of the drug in therapy or prevention regimens leads to normalization of levels of transaminases and total protein in the blood...

Citations

... To understand surface chemical composition of modified bacterial cellulose, XPS is widely used [53][54][55][56][57][58][59][60][61][62]. We analyzed and compiled literature data related to the C 1s, O 1s and Ag 3d5/2 spectra of BCF, Ag NPs/BCF, SBCB and Ag NPs/SBCB and Ag NPs (Tables 1 and 2). ...
... It was shown [46,47] that during cellulose cultivation, thin primary layers are first formed and then new thicker secondary layers are formed on top, the density of which is not uniform due to a decrease in the concentration of the carbon source over time [48]. It was found that primary layers with a diameter of about 1.3 μm are present in the structures of the obtained BCF, which are at a distance of 4-5 μm from each other, forming areas with a low density of fibrils. ...
... It was found that primary layers with a diameter of about 1.3 μm are present in the structures of the obtained BCF, which are at a distance of 4-5 μm from each other, forming areas with a low density of fibrils. The distance between the primary layers is about 4.5 μm, which is in good agreement with the previously established data [49,50]. Analysis of BCF micrographs showed that there are secondary layers with a thickness of about 13 μm and a distance between them of 12.5 μm. ...
... Fig. 2 (d1) and Fig. 2 (d2) shown the C1s spectra of the CS and CS/GEL/PA-Na respectively. There were four bands at 287.0 eV, 285.9 eV, 284.9 eV and 284.1 eV, corresponding to C-H, C-C, C-N and C-O for CS, respectively (He et al., 2021;Li et al., 2021;Li et al., 2021Li et al., , 2021Novikov et al., 2021). Those bands; however, were shifted and found at 285.2 eV, 284.8 eV, 284.5 eV, 284.1 eV in CS/GEL/PA-Na. ...
... The scanning electron micrographs presented in figure 6 reveal the internal structure on the micron scale: Here, the layers of micro-sized tubes or 'microtunnels' described and rationalized by Gromovykh et al and exhibiting a similar periodicity, were clearly visible, figure 6(b) [20]. We found that these exhibit curvatures, depending on the location within the sample, figure 6(a). ...
... Эффективность используемых методов очистки натуральных мембран БЦ с использованием только NaOH находится на этапе критического осмысления и поиска эффективных подходов для улучшения очистки БЦ. Так, рассматриваются альтернативные решения одновременной очисткой и стерилизации путем обработки мембран БЦ сверхкритическим диоксидом углерода, а также с помощью холодной плазмы [17,18]. В своей работе мы показали, что нативные мембраны БЦ в срок 7 суток после имплантации вызывают местную воспалительную реакцию экссудативного типа, которая проявляется преобладанием в составе периимплантного инфильтрата полинуклеарных лейкоцитов с участием макрофагов, что наблюдалось при всех вариантах подготовки БЦ. ...
... Kurokawa et al. [5] reported that Monascus pigments possess great anticancer activity against murine 4T1 mammary carcinoma cell, and the cell viability reduced from approximately 50% to 5% when MP concentration increased from 300 to 400 µg/mL. Compared with free silibinin, an extraction from Silybum marianum seeds, the liposomal silibinin exhibited stronger cytotoxic activity against cancer cells [40]. The same trends are observed in propolis liposomes, which powerfully enhanced the cytotoxic effect on Hep-2 cells compared with the unencapsulated ones [26]. ...
... Nowadays, large amounts of silibinin powders can be easily prepared. However, recent reports have demonstrated that silibinin can also adopt conformations that are more complex such as liposomal forms [23] and emulsomes [24]. Furthermore, it can be confined in oil nanocapsules [25], adsorbed on magnetic nanoparticles [26], and form silibinin-metal ion complexes with interesting properties [27]. ...