Taichi Kashiwagi's research while affiliated with Tokyo Medical and Dental University and other places
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Publications (17)
Background
Tissue stem cells are confined within a special microenvironment called niche. Stem cells in such a niche are supplied with nutrients and contacted by other cells to maintain their characters and also to keep or expand their population size. Besides, oxygen concentration is a key factor for stem cell niche. Adult neural stem/progenitor c...
We describe here a novel assay that determines the total a+ntioxidative activities of known antioxidants and antioxidants in beverages. The method employs the substrate 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS) that yields the colored product 3,5,3’,5’-tetrabromoazobenzene sulfate sodium salt (azo-TBBS). The amounts of azo-TBBS are measured us...
Status epilepticus (SE) is a neurological emergency, and delayed management can lead to higher morbidity and mortality. It is thought that prolonged seizures stimulate stem cells in the hippocampus and that epileptogenesis may arise from aberrant connections formed by newly born cells, while others have suggested that the acute neuroinflammation an...
Aging-related neurodegenerative disorders are closely associated with mitochondrial dysfunction and oxidative stresses and their incidence tends to increase with aging. Brain is the most vulnerable to reactive species generated by a higher rate of oxygen consumption and glucose utilization compared to other organs. Electrochemically reduced water (...
The original article has been published with an error that is corrected below: Under the heading “Mineral waters and medium preparation” in the section of Materials and methods, the sentence “NMWs have the following physical properties: for Hita T. W., pH of 7.29 ± 0.02 and Eh of 410.33 ± 10.41 mV” should be replaced by “NMWs have the following phy...
Insulin-producing cells express limited activities of anti-oxidative enzymes. Therefore, reactive oxygen species (ROS) produced in these cells play a crucial role in cytotoxic effects. Furthermore, diabetes mellitus (DM) development is closely linked to higher ROS levels in insulin-producing cells. Hita Tenryosui Water(®) (Hita T. W., Hita, Japan)...
Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage...
There are few reports on the physiological effects of metal nanoparticles (nps), especially with respect to their functions as scavengers for superoxide anion radical (O2(.-)) and hydroxyl radical (.OH). We tried to detect the scavenging activity of Pt nps using a hypoxanthine-xanthine oxidase system for O2(.-) and using a Fenton and a UV/H2O2 syst...
Vascular endothelial growth factor (VEGF) is a key mediator of tumor angiogenesis. Tumor cells are exposed to higher oxidative stress compared to normal cells. Numerous reports have demonstrated that the intracellular redox (oxidation/reduction) state is closely associated with the pattern of VEGF expression. Electrolyzed reduced water (ERW) produc...
In the two-stage cell transformation theory, cancer cells first receive initiation, which is mainly caused by DNA damage, and then promotion, which enhances transformation. Murine Balb/c 3T3 cells are widely used for transformation experiments because they lose contact inhibition ability when transformed. Electrolyzed reduced water (ERW), which is...
We have proposed an active hydrogen reduced water theory that active hydrogen produced by electrolysis of water is stabilized
in the form of hydrogenated metal nanocolloids in electrolyzed reduced water (ERW) and scavenges intracellular reactive oxygen
species (ROS). Because various brain diseases are caused by oxygen stress, we examined the effec...
Electrolysed-reduced water (ERW) contained Platinum nanocolloids (PtNCs) of 1–10 nm, suggesting that PtNCs in ERW functioned as active hydrogen donors and scavenge intracellular reactive oxygen species (ROS). Electron paramagnetic resonance (EPR) analysis revealed that synthesized PtNCs of about 2 nm scavenged superoxide anion radicals and DPPH rad...
Electrolyzed-reduced water (ERW) produced by electrolyzing water in cathode side has the ability to scavenge reactive oxygen species (ROS). Here, we investigated the effect of ERW on oxidative stress-induced neural cell death by glutamate. When cell viability assay was performed using primary rat cerebral cortical culture as neural model, ERW suppr...
Reactive oxygen species (ROS) cause irreversible damage to biological macromolecules, resulting in many diseases. Reduced water (RW) such as hydrogen-rich electrolyzed reduced water and natural reduced waters like Hita Tenryosui water in Japan and Nordenau water in Germany that are known to improve various diseases, could protect a hamster pancreat...
Citations
... Irrelevance of Alkaline pH in ERW Importantly, although ERW has an alkaline pH [1,71,72], we can logically exclude this property from being a contributing factor to its therapeutic effects because, in addition to the facts mentioned above, in all the in vitro cell culture studies, the alkaline pH is always neutralized [29,33,35,40,43,44,[73][74][75][76][77][78][79][80][81][82]. Otherwise, the high alkaline pH would have injured the cells, since a high pH is incompatible with our cellular structure and function. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/277207952969740-1443102934601_Q64/Takeki-Hamasaki.jpg)
... Since at least May 1985, there have been many documented clinical improvements from the consumption of ERW, which were thought to be miraculous at the time [27]. Around 1997, ERW research increased, and additional studies revealed that ERW has antioxidant effects [28], protects DNA from oxidative damage [17,[29][30][31], promotes GLUT4 translocation [32], protects pancreatic beta cells from alloxan-induced cell damage [33], prevents aspirin-induced gastric mucosal injury [34], suppresses lipid peroxidation [35], and has anti-diabetic [36][37][38][39] and anticancer [40][41][42][43][44] effects. ...
... It suppresses tumor angiogenesis by scavenging intracellular ROS and suppressing the gene expression and secretion of vascular endothelial growth factor (45). ERW suppresses the growth of cancer cells and microorganisms (46,47) and induces apoptosis together with glutathione in human leukemia HL60 cells (48). Hydrogenenriched water suppresses the side effects of anti-cancer drugs (1). ...
... To achieve this goal, the food and pharmaceutical industries require access to detailed data regarding the levels of antioxidants contained in food to maximize the availability of antioxidants in the body [5 , 6] . Therefore, we have been conducting in vitro and in vivo studies to identify the potential beneficial therapeutic effects of antioxidants present in food and drinking water [7][8][9][10][11][12][13][14][15][16] . ...
... ERW, also called "alkaline ionized water", is characterized by alkaline pH, low dissolved oxygen gas concentration, high dissolved hydrogen gas concentration, and a negative oxidation-reduction potential (ORP) [24]. The anti-T1DM effect of ERW has also been observed in an alloxan-induced insulin-dependent diabetic mice model [20]. Increasing evidence demonstrates that the dissolved hydrogen in ERW is exclusively responsible for any observed therapeutic effects [24]. ...
... Investigations of some "curative waters" (e.g., Nordenau, Germany; Tlacote, Mexico; Hita Tenryosui, Japan; Nadone, India, etc.) have been reported to indeed exert therapeutic effects [138][139][140]. Intriguingly, there are reports that some of these waters contain small levels of dissolved molecular hydrogen [138,[140][141][142][143][144]. In the late 1990s and early 2000s, a few procedural observational studies were performed on hundreds of diabetic patients who drank these claimed natural healing waters. ...
... Since at least May 1985, there have been many documented clinical improvements from the consumption of ERW, which were thought to be miraculous at the time [27]. Around 1997, ERW research increased, and additional studies revealed that ERW has antioxidant effects [28], protects DNA from oxidative damage [17,[29][30][31], promotes GLUT4 translocation [32], protects pancreatic beta cells from alloxan-induced cell damage [33], prevents aspirin-induced gastric mucosal injury [34], suppresses lipid peroxidation [35], and has anti-diabetic [36][37][38][39] and anticancer [40][41][42][43][44] effects. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/279951820509213-1443757123611_Q64/Ryuhei-Nishikawa.jpg)
![[object Object]](https://i1.rgstatic.net/ii/profile.image/272361517744132-1441947454116_Q64/Yoshinori-Katakura.jpg)
... Irrelevance of Alkaline pH in ERW Importantly, although ERW has an alkaline pH [1,71,72], we can logically exclude this property from being a contributing factor to its therapeutic effects because, in addition to the facts mentioned above, in all the in vitro cell culture studies, the alkaline pH is always neutralized [29,33,35,40,43,44,[73][74][75][76][77][78][79][80][81][82]. Otherwise, the high alkaline pH would have injured the cells, since a high pH is incompatible with our cellular structure and function. ...
... It is thought that nano-antioxidant therapy can address these challenges. Currently, methods using platinum nanoparticles [51], GSH-transporting nanoliposomes [52], and high-molecular-weight polymers [53] exist. These methods are useful for enhancing the capacity and specificity of antioxidants. ...
