Suzanne Crowe's research while affiliated with University of Ottawa and other places

Publications (8)

Article
Full-text available
The use of biomaterials and tracking the long-term fate of the transplanted cells is expected to help improve the clinical translation of cell therapies for cardiac regeneration. To this end, reporter gene strategies are promising for monitoring the fate of cells transplanted with or without a delivery biomaterial; however, their application with p...
Article
Circulating angiogenic cells (CACs) are a heterogeneous cell population of bone marrow (BM) origin. These cells are most commonly derived from the peripheral blood, bone marrow, and cord blood, and are one of the leading candidates for promoting vascularization in tissue engineering therapies. CACs can be isolated by culturing peripheral blood mono...
Article
The mechanisms involved in myocardial regeneration and cardiac remodeling were examined by injecting porcine-derived small intestine submucosal extracellular matrix (SIS-ECM), with and without circulating angiogenic cells (CACs), in a mouse model of acute myocardial infarction (MI). Nine- to 10-week-old female C57BL/6J mice had the left anterior de...
Article
Full-text available
Cell transplantation for regenerative medicine has become an appealing therapeutic method; however, stem and progenitor cells are not always freshly available. Cryopreservation offers a way to freeze cells as they are generated, for storage and transport until required for therapy. This study was performed to assess the feasibility of cryopreservin...
Article
In cardiac cell therapy almost every cell type tested experimentally has yielded some benefit. However, there is a lack of studies directly comparing the function of various stem/progenitor cell populations. This study describes the expansion of peripheral blood CD133(+) cells and compares their functional properties with those of other commonly us...

Citations

... The featured reviews present recent progress in assembling cells and matrix into functional tissues by 3D printing [12] , and microfabrication for the purposes of personalization, disease modelling and drug discovery [6,13]. Once the cells are injected or placed with a biomaterial matrix at the desired site in the heart, their fate needs to be tracked in vivo and this special section brings an original paper on in vivo tracking of angiogenic cells transplanted into rodent hearts [14]. Finally, the progress of in vivo pre-clinical studies with engineered cardiac tissues on biomaterial matrices are reviewed [15]. ...
... One good example of this is the circulating angiogenic cells. These are heterogeneous cell populations of bone marrow origin that possess angiogenic properties, and have been demonstrated to promote vascularization in tissue engineering therapies [12,13]. Overall, co-cultures are demonstrated to be important in driving proper cell growth and for generating compacted 3D tissues via direct cell-cell contact, cell-ECM interaction, and signaling with soluble factors, making them an essential component of the biomimetic cardiac microenvironment [14,15]. ...
... Although we have not used native cardiac tissue ECM, we show later that bECM can solve some of the challenges we have raised, and it can be employed as a cell actuating biomaterial beyond cardiac tissue engineering applications. These materials have also been previously reported in the literature for applications in cardiac tissue repair and engineering (Ravi et al., 2012;Toeg et al., 2013). In the case of sisECM, cells were removed by mechanical delamination. ...
... All tissue samples were sectioned and randomly allocated to immediate cell culture (fresh tissue), immediate cryopreservation or delayed cryopreservation after 24 hours of refrigeration (4˚C) in cardioplegia solution (lactated ringers, 2% St Thomas solution, 5 mEq NaHCO3 and 10 mEq KCl; Thermo Fisher Scientific). Tissue samples were cryopreserved to -80˚C within 5% dimethyl sulfoxide, 6% fetal bovine serum within Iscove's Modified Dulbecco's Medium [9]. One month later, cryopreserved tissue specimen vials were recovered in a 37˚C water bath prior to processing. ...
... Taken together, sometimes CD133 phenotypic marker is useful. For example, it could be advantageous for inducing vascular-creation in the ischemic heart cells through transplantation (Zhang et al., 2010). ...