Sung In Jeong's research while affiliated with Korea Atomic Energy Research Institute (KAERI) and other places
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Publications (53)
Conductive polymers, including polypyrrole (PPy), have been extensively explored to fabricate electrically conductive biomaterials for bioelectrodes and tissue engineering scaffolds. For their in vivo uses, a sterilization method without severe impairment of original material properties and performance is necessary. Gamma-ray radiation has been com...
Introduction
Although numerous studies have been conducted with the aim of developing drug-delivery systems, chemically synthesized gene carriers have shown limited applications in the biomedical fields due to several problems, such as low-grafting yields, undesirable reactions, difficulties in controlling the reactions, and high-cost production ow...
Bacterial cellulose (BC) is an excellent biomaterial with many medical applications. In this study, resorbable BC membranes were prepared for guided bone regeneration (GBR) using an irradiation technique for applications in the dental field. Electron beam irradiation (EI) increases biodegradation by severing the glucose bonds of BC. BC membranes ir...
Hydrogels consisted of β-glucan, poly(vinyl alcohol) (PVA), polyvinyl pyrrolidone) (PVP), K-carrageenan (kC) and glycerin were prepared by gamma-ray irradiation for damaged tissue regeneration. Irradiation doses of 25, 50, and 75 kGy were exposed, respectively, to the β-glucan hydrogel to evaluate the effect of irradiation dose on physical properti...
Nanofibrous scaffolds for tissue regeneration need to be three-dimensional and highly porous to support uniform cell attachment and proliferation, and need to have an interconnected and permeable pore network to promote nutrient and waste exchange. Our laboratory recently developed heparin and 2-aminoethyl methacrylate (AEMA) immobilized bacterial...
This study introduces the effect of the thickness of a bacterial cellulose membrane by comparing the bone regeneration effect on rat skulls when using a collagen membrane and different thicknesses of resorbable bacterial cellulose membranes for guided bone regeneration. Barrier membranes of 0.10 mm, 0.15 mm, and 0.20 mm in thickness were made using...
Polycaprolactone (PCL) has been considered a useful material for orthopedic devices and osseous implants because of its biocompatibility and bone-forming activity. However, PCL-based scaffolds have hydrophobic surfaces that reduce initial cell viability. In this study, we fabricated surface-modified PCL nanofibers for tissue engineering using radia...
In this study, we examined whether porcine articular cartilage (PAC) is a suitable and effective anti-adhesive material. PAC, which contained no non-collagenous tissue components, was collected by mechanical manipulation and decellularization of porcine knee cartilage. The PAC film for use as an anti-adhesive barrier was easily shaped into various...
Purpose:
This study was to evaluate the effects of bacterial cellulose (BC) membranes as a barrier membrane on guided bone regeneration (GBR) in comparison with those of the resorbable collagen membranes.
Materials and methods:
BC membranes were fabricated using biomimetic technology. Surface properties were analyzed, Mechanical properties were...
Bacterial cellulose (BC) has been shown to have a high-burst pressure, high-water contact, and ultrafine highly nanofibrous structure similar with that in a natural extracellular matrix (ECM). In the present study, we developed a BC-based functional scaffold for tissue engineering using radiation technology. BC was generated by Gluconacetobacter ha...
The goal of this study was to develop a novel hydroxyapatite (HA) coated bacterial cellulose (BC) scaffold for bone tissue regeneration. HA-coated BC was prepared by immersing in 30 mL of 5× simulated body fluid at 37°C for 12 h. The resulting HA-coated BC scaffolds were characterized by scanning electron microscopy (SEM), attenuated total reflecta...
In this work, we present a biomimetic fibrous scaffold containing two biomolecules. Biocompatible poly (L-lactide-co-ɛ-caprolactone) mesh was fabricated by an electrospinning method, and then acrylic acid was grafted on the mesh to introduce a carboxyl group through γ-ray irradiation. Subsequently, epidermal growth factor (EGF) and gelatin were cou...
Polycaprolactone (PCL) and biphasic calcium phosphate (BCP) have been considered as useful materials for orthopedic devices and osseous implants because of their biocompatibility and bone-forming activity. However, PCL-based scaffolds have hydrophobic surfaces reducing initial cell adhesion or proliferation. To overcome the limitation, we fabricate...
Alginate hydrogel has been used as an attractive scaffold for tissue regeneration. In particular, its simple cross-linking, high water absorption, and biocompatibility have provided its utility in regulating an interaction with cells or organs. However, three-dimensional (3D) networks of alginate hydrogel do not provide fibrous anchorage sites such...
There is a growing interest in bone graft materials for enhancing bone formation. We have focused on bone xenograft using animal by-products. Among animal by-products, the duck beak was specifically used in this study for manufacture of bone graft particles. Harvested duck-beak bone was defatted, deproteinized, and then heat-treated at 350ΰC. Subse...
A significant problem affecting electrospun nanofibrous tissue scaffolds is poor infiltration of cells into their three-dimensional (3D) structure. Environmental and physical manipulation, however, can enhance cellular infiltration into electrospun scaffolds. In this work, RGD-modified alginate mats with increased thickness and porosity were achiev...
Bacterial cellulose(BC) scaffolds are generated from gel by gluconacetobacter hansenii TL-2C. BC has good properties such as high-burst pressure and the ultrafine highly nanofibrous structure of mimic natural extra celluar matrix(ECM) for tissue engineering. In this study, 2-aminoethyl methacrylate(AEMA) was grafted onto BC surface using gamma-irra...
Over the last decade, bone tissue engineering scaffolds have been advanced owing to the bioceramic incorporation and biomimetic modification. In this report, a dual-functional fibrous scaffold with a bioceramic and biomolecule is developed, and a combined effect of a dual-modification is investigated. Biphasic calcium phosphate (BCP) is incorporate...
Alginate, a linear un-branched polysaccharide derived from seaweed has shown great potential as a cell scaffold for the regeneration of many tissues. However, alginate is not naturally enzymatically degraded in ionically crossliked alginate htdrogels exhibit a remarkably slow degradation rate, which is typically months to years for their complete r...
An artificial construct mimicking the intrinsic properties of the natural extracellular matrix in bones has been considered an ideal platform for bone tissue engineering, as it can present an appropriate microenvironment and regulate cell behaviours. In this report, we introduce biodegradable composite scaffolds consisting of polycaprolactone (PCL)...
One of the interesting strategies for developing the artificial blood vessels is to generate multi-layered scaffolds for mimicking the structure of native blood vessels such as the intima, media, and adventitia. In this study, we prepared dual-layered poly(L-lactide-co-ɛ-caprolactone) (PLCL) scaffolds with micro- and nanofibers as a basic construct...
In this study, polysaccharide-poly(vinyl alcohol) (PVA) hydrogels were prepared by using -ray and evaluated for potential application as an anti-inflammation patch. Ulmus davidiana var. japonica (UD), one of polysaccharides has been particularly used as an oriental remedy for the treatment of inflammation and ulcers. PVA as a biocompatible polymer...
Propionibacterium acnes (P. acnes), one of the anaerobic bacterium,
causes inflammatory acne. To find a novel medication for treating the
inflammation caused by P. acnes, we investigated the anti-bacterial and
anti-inflammatory activities of several herbal extracts against P.
acnes. The aqueous extracts from five dried herbs, Phellodendron
amurense...
Nanofibrous scaffolds are of interest in tissue engineering due to their high surface area to volume ratio, interconnected pores, and architectural similarity to the native extracellular matrix. Our laboratory recently developed a biodegradable, photo-crosslinkable alginate biopolymer. Here, we show the capacity of the material to be electrospun in...
The formation of 3D electrospun mat structures from alginate-polyethylene oxide (PEO) solution blends is reported. These unique architectures expand the capabilities of traditional electrospun mats for applications such as regenerative medicine, where a scaffold can help to promote tissue growth in three dimensions. The mat structures extend off th...
Blends of PAni and PLCL are electrospun to prepare uniform fibers for the development of electrically conductive, engineered nerve grafts. PC12 cell viability is significantly higher on RPACL fibers than on PLCL-only fibers, and the electrical conductivity of the fibers affects the differentiation of PC12 cells; the number of cells positively-stain...
Electrospun Nanofiber sheets have been shown to mimic the structure of extracellular matrix (ECM). Although these nanofibers have shown great potential for use as tissue engineering scaffolds, it is difficult for the electrospun nanofiber based sheets to be shaped into the desired three-dimensional structure. In this study, poly(L-lactic acid) (PLL...
A significant problem that affects tissue-engineered electrospun nanofibrous scaffolds is poor infiltration of cells into the three-dimensional (3D) structure. Physical manipulation can enhance cellular infiltration into electrospun scaffolds. The porosity of electrospun nanofibers was highly enlarged by ultrasonication in an aqueous solution. The...
We report here preparation of nanofibers containing alginate using two different molecular weights (MWs): 37kDa and 196kDa. Low MW alginates are attractive for in vivo tissue scaffolds where degradation and clearance from the body are desirable, whereas higher MW alginates are amenable for topical use as wound coverage because of its better mechani...
Electrospun natural biopolymers are of great interest in the field of regenerative medicine due to their unique structure, biocompatibility, and potential to support controlled release of bioactive agents and/or the growth of cells near a site of interest. The ability to electrospin chitosan and alginate to form polyionic complexed nanofibrous scaf...
Alginate, a natural polysaccharide that has shown great potential as a cell scaffold for the regeneration of many tissues, has only been nominally explored as an electrospun biomaterial due to cytotoxic chemicals that have typically been used during nanofiber formation and crosslinking. Alginate cannot be electrospun by itself and is often co‐spun...
Novel porous scaffolds composed of gelatin/κ-carrageenan (GC) were fabricated by freeze-drying followed by chemical cross-linking with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
(EDC). The morphology of the insoluble GC sponges was examined by field emission scanning electron microscopy (FE-SEM). The
porosity of the GC sponge increased with incr...
In conclusion, we developed a DES with PLCL and paclitaxel. After expansion with a balloon, there was no evidence of cracks
or peelings in the PLCL/paclitaxel coated stent. Paclitaxel was releasedin vitro up to 96% relative to the total loading weight over 50 days in a PLCL concentrationdependent manner and elutedin vivo up to 97.3% for 30 days. Fr...
Developing biomaterial scaffolds to elicit specific cell responses is important in many tissue engineering applications. We hypothesized that the chemical composition of the scaffold may be a key determinant for the effective induction of differentiation in human mesenchymal stem cells (hMSCs). In this study, electrospun nanofibers with different c...
Cardiac tissue engineering has been proposed as an appropriate method to repair myocardial infarction (MI). Evidence suggests that a cell with scaffold combination was more effective than a cell-only implant. Nevertheless, to date, there has been no research into elastic biodegradable poly(lactide-co-epsilon-caprolactone) (PLCL) scaffolds. The aim...
The effect of NBM incorporation in PLA nanofibers on their mechanical properties and the differentiation and mineralization of osteoblasts was studied. At 20% NBM, the Young's modulus of the nanofibers was 37.78 ± 4.23, significantly larger than that of pure PLA nanofibers. MC3T3‐E1 pre‐osteoblasts attached to both types of nanofibers and developed...
Tissue engineering has become an alternative method to traditional surgical treatments for the repair of bone defects, and an appropriate scaffold supporting bone formation is a key element in this approach. In the present study, nanofibrous organic and inorganic composite scaffolds containing nano-sized demineralized bone powders (DBPs) with biode...
Conclusions In this study, we demonstrate that cyclic strain increased the proliferation of HDFs and subsequent expression of ECM proteins.
Specifically, HDFs engineered under the cyclic stretching exhibited higher proliferation and up-regulated expression of collagen
type IV and fibronectin. These cellular features could allow the engineered HDF t...
In this work, electrically conductive polyaniline (PAni) doped with camphorsulfonic acid (CPSA) is blended with poly( L ‐lactide‐ co ‐ ε ‐caprolactone) (PLCL), and then electrospun to prepare uniform nanofibers. The CPSA‐PAni/PLCL nanofibers show a smooth fiber structure without coarse lumps or beads and consistent fiber diameters (which range from...
The production of nanofibrous PLA/HA composite scaffolds is described. The morphological, mechanical, surface, and thermal properties of the composites were extensively investigated. The results show that the mixture of PLA and HA formed smooth nanofibers without lumps. The incorporation of HA increased the mechanical strength of the nanofibers and...
Recently, much attention has been given to the fabrication of tissue-engineering scaffolds with nano-scaled structure to stimulate cell adhesion and proliferation in a microenvironment similar to the natural extracellular matrix milieu. In the present study, blends of gelatin and poly(L-lactide-co-epsilon-caprolactone) (PLCL) (blending ratio: 0, 30...
Novel tubular scaffolds of marine source collagen and PLGA fibers were fabricated by freeze drying and electrospinning processes for vascular grafts. The hybrid scaffolds, composed of a porous collagen matrix and a fibrous PLGA layer, had an average pore size of 150+/-50 microm. The electrospun fibrous PLGA layer on the surface of a porous tubular...
Blood vessels are subjected in vivo to mechanical forces in a form of radial distention, encompassing cyclic mechanical strain due to the pulsatile nature of blood flow. Vascular smooth muscle (VSM) tissues engineered in vitro with a conventional tissue engineering technique may not be functional, because vascular smooth muscle cells (VSMCs) cultur...
Tubular scaffolds were fabricated from very elastic poly(L-lactide-co-epsilon-caprolactone) (PLCL, 50:50). The scaffolds were seeded with smooth muscle cells (SMCs) and implanted in nude mice to investigate the tissue compatibility and in vivo degradation behavior. Histological examination of all the implants with haematoxylin and eosin staining, m...
Very elastic PLCL [poly(L-lactide-co-epsilon-caprolactone), 50:50] copolymers were synthesized and extruded into porous tubular scaffolds (pore size 150 +/- 50 microm, porosity 90%) for the application to tissue engineering. The copolymers were basically random and amorphous. However, two T(g)'s (glass transition temperatures) were observed in dyna...
A soft and very elastic poly(lactide-co-epsilon-caprolactone) (PLCL)(50:50, Mn 185 x 10(3)) was synthesized. Tubular scaffolds were prepared by an extrusion-particulate leaching method for mechano-active vascular tissue engineering. The copolymer was very flexible but completely rubber-like elastic. Even the high porous PLCL scaffolds (90% salt wt)...
Cyclic mechanical strain has been demonstrated to enhance the development and function of engineered smooth muscle (SM) tissues, and it would be necessary for the development of the elastic scaffolds if one wishes to engineer SM tissues under cyclic mechanical loading. This study reports on the development of an elastic scaffold fabricated from a b...
Citations
... In addition, they are commonly used for the irradiation of foods to improve their quality and extend shelf life. Also, gamma-ray irradiation is commonly applied in sterilizing medical products due to its simple sterilization without heat generation [25]. A COVID-19 rapid letter stated by Venkatraman et al. [26] proposed an idea of how gamma rays are used to breakthrough COVID-19 to manage its outbreak. ...
... The overall classifications were tabulated with their relevant references, and the class percentage data was graphically presented. The applications, which have very rare publications, such as gene delivery [49,50], were ignored, although they might be appealing for preparation with other methods falling beyond the scope of this article. ...
... The FTIR spectrum (Figure 4) characteristic of pure BNC is marked by bands in the region of 3345 cm -1 , which according to He et al. [25] , is characteristic of the stretching of the hydroxyl groups present in BNC. In addition, the CH stretching and asymmetric stretching at 2897 cm -1 , CH 2 deformation at 1427 cm -1 , OH deformation at 1315 and 1359 cm -1 , as well as the antisymmetric bridge of the C-O-C stretch at 1109 and 1162 cm -1 and the band in the region of 1056 cm -1 related to the vibrations of the C-O stretch are characteristic of BNC [26,27] . Another exciting band, located around 400 to 700 cm -1 , is characteristic of the torsion of the OH groups. ...
... Among the studied irradiation doses, 50 kGy proved to be an effective dose, allowing the maintenance of excellent antioxidant and functional properties. Further, the physical properties (e.g., gel fraction, compressive strength) of irradiated β-glucans were enhanced by increasing the irradiation dose [41]. Taken together, irradiated β-glucan exhibits: (a) a higher antimicrobial activity compared to the native samples; (b) an enhanced antioxidant, antitumor and antiproliferative activity; and finally, (c) an enhanced response in the immune system, suggesting that smaller molecules of irradiated β-glucans have a higher chance of binding to a receptor. ...
... The thicker membranes were those produced by K. xylinus DSM 46604 from glucose as the sole carbon source (44 ± 2.2 µm) and in the glucose/styrene mixtures (22 ± 1.1 µm). These values are among those reported for BC membranes (20-200 µm) (Peres et al. 2016;Lee et al. 2017;Vázquez et al. 2021). However, considerably lower thickness values were observed for K. xylinus DSM 2325 and K. xylinus DSM 2004 BC produced in the glucose/styrene mixtures (6 ± 0.3 and 7 ± 0.4 µm, respectively), while even lower values were displayed by the BC membranes produced by K. xylinus DSM 2004 grown on styrene as sole carbon source (3 ± 0.2 µm) ( Table 2), which is concomitant with the lower BC production observed in these conditions. ...
... Gamma-ray irradiation can induce various chemical reactions (e.g., polymerization, decomposition, surface modification, and crosslinking) using radical recombination reactions due to their high energy and self-ionization. In addition, it has the advantage of being able to induce reactions in various environments (e.g., oxygen, nitrogen, and vacuum) and states (e.g., solids and liquids) [34,43,44]. Various reactions can be induced by controlling the polymer properties and the conditions of the gamma-ray-irradiation dose and dose rate. ...
... Lee et al., for example, coated HA with b-cyclodextrin (b-CD) to improve the binding of drugs such as adamantane and simvastatin, given that b-CD was shown to mediate the drug-HA interaction. The resulting composite was able to enhance the osteogenic differentiation of adipose-derived stromal cells [84,85]. ...
... Several clinical [46] and experimental studies [47] have demonstrated the effectiveness of this membrane as a biological barrier in significant bone defects-either combined with a biomaterial, or used in isolation. This guided bone regeneration (GBR) technique is gradually being applied in clinical practice to effectively compensate for the lack of bone mass [48]. ...
... Cartilage-derived ECM also known as cartilage acellular matrix (CAM) is complex and similar to that of natural cartilage [29]. It facilitates the regeneration of mature cartilage. ...
... The results show that PDA increases the mechanical strength of the BC membrane, while the PLGAcoated BC membrane improves cell proliferation and collagen accumulation in vivo. Lim et al. have successfully prepared new hydroxyapatite-encapsulated BC scaffolds for bone-tissue engineering (Ahn et al., 2015). New bone formation was observed to be significantly increased by hydroxyapatite-coated BC scaffolds in a rat calvarian defect model compared with those from BC scaffolds. ...