Subramanium Vennila’s research while affiliated with King Institute of Preventive Medicine and other places

Background: Diabetes mellitus patients thrive very hard to reduce their glucose levels by using various hypoglycemic agents. Many hypoglycemic agents cause side effects and fail to reduce glucose level, when they use for a long time. Folklore natural medicines will successfully act on many diseases without causing any side effects when it was consumes prolonged. Patients practicing yoga and exercise with the traditional medicines contribute a significant efficacy in management of diabetes in very short period. Main Observations: We report the cases of 40 to 60 year old type-2 diabetic patients were evaluated for the efficacy of Helicteres isora concoction 5 mg/5 ml concentration with practicing yoga and exercise. The patients were observed for the glucose levels and body weight before evaluating with cohort study. The results from the cohort study showed that the group 1M and 1O had significant reduction in glucose levels when compared to control groups treated for four weeks. Conclusions: Type-2 Diabetic Patients administered orally with H.isora concoction in practicing yoga reveals that they were significantly reduced in blood glucose levels and body weight. Thus the study provides a novel hypoglycemic agent which is very cheaper that ails to the management of diabetes mellitus.

Insulin receptor (IR) proteins were essential intracellular signaling peptides in the insulin action cascade. Insulin receptor substrate proteins (IRS-1and IRS-2) serve and regulate the insulin level in the normal insulin action. The broad role of IRS-1 and IRS-2 in cell growth and survival reveals a common regulatory pathway linking development, somatic growth, fertility, neuronal proliferation, and aging to the core mechanisms used by vertebrates for nutrient sensing. Such type of proteins were cyclic adenosine monophosphate-activated protein kinase, this proteins play a key role in the insulin response and regulation. Type -2 Diabetes mellitus occurs during prolonged periods of peripheral insulin resistance due to inactivation of IRS proteins. The compounds isolated from the medicinal plants were safer than synthetic drugs and possess high bio activity. In the present study, four compounds were elucidated from fruits of Helicteres isora. The elucidated compounds were evaluated for the antidiabetic activity using Insilico docking study. The receptor was analyzed for the active site and pocket finder tools. The aminoacids such as Phenylalanine, Lysine, Glutamic acid and Asparigine were predicted as active site binding residues. Docking studies were done through Autodock 4 software. All the compounds from fruits of Helicteres isora showed good docking profiles with AMP Kinase, except compound-3 (1,2,3,4-tetrahydro-1,5,6,8-tetramethyl-7-(2-methylprop-1-enylnaphthalene-4-ylpivalate). Finally the result from the study demonstrates that the HS-1, HS-2 and HS-4 posses potent anti diabetic activity against type-2 diabetes mellitus through drug action on AMP kinase cascade system.