Steven Janvier's research while affiliated with Vlaams Instituut voor Biotechnologie and other places

Publications (14)

Article
Full-text available
Intrinsically disordered proteins (IDPs) lack well-defined 3D structures and can only be described as ensembles of different conformations. This high degree of flexibility allows them to interact promiscuously and makes them capable of fulfilling unique and versatile regulatory roles in cellular processes. These functional benefits make IDPs widesp...
Article
At the end of 2017 and 2018 two different unknown suspicious preparations were encountered and were subjected to a plethora of different analyses in order to identify, if present, any bioactive compound. It turned out that these samples contained the assumedly cognitive enhancing research peptides Selank and Semax, which, to our knowledge, have not...
Article
The aim of the present study was to monitor the consumption of foods containing intense sweeteners present on the Italian food market and to investigate whether the Italian general population (aged >3-65+) was at risk for exceeding the Acceptable Daily Intake (ADI) of 10 intense sweeteners. A food label survey was performed in Rome (Italy), using m...
Data
Supplement Fig. 1. Total ion chromatograms of (A) insulin detemir, (B) insulin degludec. The black chromatogram corresponds to the untreated insulin and the red chromatogram corresponds to the acid and heat treated form. The TIC shown in (C) show that the later eluting peaks of detemir and degludec are not an artifact due to the treatment since the...
Article
Full-text available
The occurrence of illegal medicines is a well-established global problem and concerns mostly small molecules. However, due to the advances in genomics and recombinant expression technologies there is an increased development of polypeptide therapeutics. Insulin is one of the best known polypeptide drug and illegal versions of this medicine have alr...
Article
Full-text available
This study determines the occurrence and concentration levels of artificial low calorie sweeteners (LCS) in food and food supplements on the Italian market. The analysed sample set (290 samples) was representative for the Italian market and comprised of beverages, jams, ketchups, confectionery, dairy products, table-top sweeteners and food suppleme...

Citations

... A recent paper on the subject can be found in reference Biesterbos et al. 13 of which were discarded during drug discovery studies due to their side effects/disadvantageous benefit-risk ratio, 16 or molecules that are still under clinical investigation or that failed clinical trials. [17][18][19][20][21] Needless to say, the use of unapproved medicines, for which no or very little toxicological or clinical data is available, potentially represents a high health risk for the patient or consumer. Furthermore, when using dietary supplements, herbal medicines, cosmetics and so forth, the consumer is very often not aware that they are taking a medicine, and especially not a non-approved medicine. ...
... The SF medicine problem cannot be narrowed down just to sexual-and other performance-enhancing medications. Several other therapeutic categories and even life-saving medicines are affected (e.g., antibiotics and antimalarials, oncotherapeutics, biological drugs, and cardiovascular medicines) (Kelesidis and Falagas, 2015;Janvier et al., 2018;Ozawa et al., 2018;Venhuis et al., 2018;Eberle et al., 2020;Tesfaye et al., 2020;Świeczkowski et al., 2022). Therefore, the health effects or harms associated with SF medicines are also showing a wide range of possibilities, such as treatment failure, toxicity, and antimicrobial resistance, making it even harder to identify. ...
... World Health Organization (WHO) defines a counterfeit drug as one "which is deliberately and fraudulently mislabeled with respect to identity and/or source" [29]. In the developing world illegal actions concentrate on lifesaving medicines, such as anti-malaria drugs, whereas in high income countries, performance and image enhancing drugs (PIEDs), including lifestyle drugs, such as phosphodiesterase type-5 (PDE-5is) inhibitors for erectile dysfunction (ED), are targeted [30]. The analysis of multiple isobaric peaks by LCMS QTOF proved to be highly efficient in prediction of the API origin and was suggested as a promising tool in tracing drug sources [35]. ...
... Numerous reports demonstrate the popularity of these peptides, as reviewed by Janvier et al. 1 These products are mainly used to improve sports performance or to strive to comply to the current cultural and societal ideals (e.g. the use of weight loss enhancers and the use of tanning peptides in Western and Northern Europe). The danger of the illegal usage of these polypeptides resides in the fact that these products are not necessarily produced under a controlled environment and consequently could contain the wrong ingredient, the wrong dosage, pathogenic bacteria, heavy metals, etc., [1][2][3][4] which may result in severe health issues. Furthermore, for many of these polypeptide drugs, there is no, or limited, knowledge of their effects and/or side-effects (long and short term) hence clinical studies have not even been initiated, are ongoing, or were given a negative advice due to the overall potential adverse health effects. ...
... Additionally, linearity was assessed for TeNT concentrations ranging from 0.25 to 25 Lf/ml by applying least squares regression analysis. Adequate linearity was achieved when the regression coefficient (r) ≥ 0.99 and non-linearity was tested with a Mandel's fitting test as described in [35,36]. implies that 95 % of the results will fall within the TE limit, given a Gaussian distribution. ...
... Studies on dietary exposure to steviol glycosides in Europe are limited (24)(25)(26)(27) . A global review of dietary intake of lowcalorie sweeteners was recently conducted by Martyn et al. (28) . ...
... The most abundantly detected peptides for the light chain (GLDIYYK) and heavy chain (VGYNAPGIPLYK) respectively were retained. Moreover, we confirmed that these peptides were indeed present in 3 independent tryptic digest experiments of the purified TeNT that were performed according to the previously described settings [47]. Accordingly, heavy isotope labeled versions of those peptides, GLD(I*)YYK and VGYNAPG(I*)PLYK, were synthesized and used as the internal standards (IS). ...
... According to Vanhee et al. [14], insulin demand is crucially increasing, which has led to finding alternative ways to meet it illegally, such as having counterfeit insulin obtained from illegal markets. Other reasons for counterfeiting insulin are the increasing number of diabetic patients in an uncontrollable manner, which has resulted in a lower supply and higher demand, as well as the growth in the market revenue for insulin in the last few years, which has incentivized counterfeiters seeking illegal profits. ...
... Three studies that published data on concentrations of neohesperidine dihydrochalcone (E 959) in representative food samples were for the Spanish (Lorenzo et al., 2015), Italian (Janvier et al., 2015) and Polish (Zygler et al., 2012) markets. In all these studies, neohesperidine dihydrochalcone (E 959) was quantified in only a limited number of samples. ...
... In the last decades, the market of biopharmaceuticals, including protein-, peptideand oligonucleotide-based therapeutics, has experienced an impressive increase due to the biological properties of this new class of molecules being potentially promising for medical applications [1][2][3][4]. Recent advancements in the production strategies, such as in genomics, proteomics recombinant strategies and peptide synthesis, have pushed even further the development of these new drugs [5][6][7][8]. Compared to traditional small molecules, biopharmaceuticals exhibit higher specificity and potency, which derive from their complex three-dimensional structures [9,10]; in addition, their action results are effective even at very-low concentrations [11]. ...