Sonja Schipperges's scientific contributions

Publications (4)

Article
In comprehensive two-dimensional liquid chromatography (LC × LC), solvents of high eluotropic strength are frequently used in the first dimension (1D), which lead to peak broadening in the second dimension (2D). In the majority of the current LC × LC column combinations, analytes are less than optimally refocused upon transfer to the second column,...
Article
Phenolic compounds are an interesting class of natural products because of their proposed contribution to health benefits of foods and beverages and as a bio-source of organic (aromatic) building blocks. Phenolic extracts from natural products are often highly complex and contain compounds covering a broad range in molecular properties. While many...
Article
In this study, the possibilities of temperature responsive × reversed phase liquid chromatography (TRLC × RPLC) are assessed in terms of pharmaceutical impurity analysis. Due to the increased peak capacity per unit time they offer, two-dimensional LC approaches are gaining relevance for the analysis of complex drug formulations. Because the latter...
Article
In this study a heart-cutting 2D-LC method was successfully developed and optimized in order to discriminate and quantitate (S)-propranolol, (R)-propranolol, and its hydroxy metabolites, namely the isomeric (S)-4′‑hydroxy propranolol, (R)-4′‑hydroxy propranolol, (S)-5′‑hydroxy propranolol, (R)-5′‑hydroxy propranolol, (S)-7′-hydroxy propranolol, and...

Citations

... The most commonly used phase systems are RP × RP followed by a combination of RP with hydrophilic interaction liquid chromatography. Although the two-dimensional LC method has not yet been reported for the analysis of polyphenolic compounds in balsamic vinegar samples, it is widely applied for the analysis of polyphenols in grapes and other products of grapes [20,21], for example, wine samples [22,23]. ...
... The use of comprehensive two-dimensional liquid chromatography (LC×LC) has considerably grown in the last decade [1]. Instrumental developments (for instance, modulation interfaces [2]) have facilitated the implementation of multidimensional liquid chromatography in various research fields, such as food [3,4], environmental [5,6], traditional Chinese medicine [7], pharmaceutical [8,9], proteomic [10,11], or metabolomic [12,13] analysis, among many others. However, regardless of the latest improvements in LC×LC, data analysis remains a major bottleneck due to the high complexity and huge size of the generated datasets. ...
... Thus, analysis of the glucuronidation of this substrate by a UGT allows the relevant reaction site of 4-hydroxypropranolol for the same enzyme to be inferred. 4-Methoxypropranolol was synthesized chemically according to a method adapted from Oatis et al. and Harps et al. [33,34] (Supplementary Material). The synthesis was stopped before the final step of demethylation, and thus, 4-methoxypropranolol was obtained. ...