Song Yang's research while affiliated with University of Georgia and other places

Publications (5)

Article
Very late factor 1 (VLF-1) of Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV) activates the transcription of two genes, polyhedrin (polh) and p10, during the final, occlusion-specific phase of infection. Using transient expression assays responsive to VLF-1, we identified linker scan mutations in the polh and p10 promoters wh...
Article
Recombinant baculoviruses which can be linearized at unique sites with restriction endonucleases can greatly facilitate the construction of other recombinants including baculovirus expression vectors and site-specific mutants. We designed a strategy to introduce unique restriction endonuclease sites at virtually any location in a baculovirus genome...
Article
vlf-1 is a baculovirus gene that regulates very late gene expression (J. R. McLachlin and L. K. Miller, J. Virol., 68, 7746-7756, 1994) and also plays a crucial role in the replication of the budded form of Autographa californica nuclear polyhedrosis virus (AcMNPV) (S. Yang and L. K. Miller, "Expression and mutational analysis of the baculovirus ve...
Article
We have found that a temperature-sensitive mutant of the baculovirus AcMNPV, tsB97, is defective in PKIP, the product of ORF24 which was previously found to interact with and stimulate the activity of a virus-encoded protein kinase, PK-1. The mutant lacks the ability to form plaques and occlusion bodies at the nonpermissive temperature. The mutant...
Article
We have examined the expression and function of a gene, vlf-1, of Autographa californica nuclear polyhedrosis virus that is known to encode a regulator of very late gene transcription. Western blot analysis revealed that vlf-1 is expressed during the late phase of infection, primarily from 15 to 24 h postinfection. VLF-1 localized in the cell nucle...

Citations

... The basal structure may consist of VLF-1 [37][38][39], BV/ODV-C42 [40], P78/83 [40], BV/ODV-EC27 [41], and other proteins. VLF-1 is a structural protein that is detected in two types of virions (BV and ODV) [42] and localizes to the ends of nucleocapsid [37]. P78/83 is a Wiskott-Aldrich syndrome protein (WASP)-like protein that activates Arp2/3 to drive the polymerization of G-actin into F-actin. ...
... PKIP is a protein that interacts with PK1 to stimulate the activity of PK1. A temperature-sensitive mutation in the pkip gene (amino acid A46 was mutated to T46) caused AcMNPV to lose its ability to form plaques and polyhedra at a non-permissive temperature of 33 °C upon the delayed synthesis of polyhedrin 50 . In the present study, PK1 and PKIP were identified by mass spectrometry (Table 2). ...
... Moreover, lef-4, lef-8, lef-9, and p47 form a minimal complex with late polymerase activity [43]. Additionally, a conserved gene, vlf-1 might, regulates very late gene transcription and may be involved in DNA processing [44][45][46]. These genes had high shared identities with AcMNPV, at 84-98%, suggesting that a similar mechanism for late gene transcription might occur in the Baculoviridae group. ...
... For instance, P47, LEF8, and LEF9 were the subunits of viral RNA polymerases for polh and p10 transcription [35]. VLF-1 activated the transcription of polh and p10 by specifically binding to the promoter burst sequence in vitro [36]. To some extent, the above results indicated that the bands we selected were reliable. ...